Click here to switch to the map view.

The map label for this gene is xlyB [H]

Identifier: 218231528

GI number: 218231528

Start: 3522255

End: 3523241

Strand: Reverse

Name: xlyB [H]

Synonym: BCB4264_A3617

Alternate gene names: 218231528

Gene position: 3523241-3522255 (Counterclockwise)

Preceding gene: 218232638

Following gene: 218231056

Centisome position: 65.02

GC content: 39.11

Gene sequence:

>987_bases
ATGGCCATTCCAGTTATACAAAATCTTGTGGCTGCAAGTAAATATCCAATCAAAGCTCCTTATTCTATGAACGCAGAGTA
TATTACAGTGCATAATACGTATAATGATGCTCCTGCAAAAAATGAAATAAATTACATGATTGGAAATAACAATGAGGTTT
CATTTCATTTTGCAGTAGATGATATTGAAGTTATACAGGGTATTCCAGTTAATCGTAATGCTTGGCATTGTGGAGATGGC
GGTGGGAATGGAAACCGAAAATCTATCGGTGTTGAAATCTGTTACTCGTTGTCTGGTGGTCCAAGATACGAAAAAGCTGA
AAGTTTAGCAATTAAATTCGTCGCTCAACTGTTACGAGAACGCGGATGGGGAATTGATCGTGTGCGTACTCATAAGAGTT
GGACAGAGATTGGTGTTCGAAACGGTAATTCTAGAGCAGTTAAGAATTGCCCACACCGTATCTTAGATGAAGGGCGCTGG
AATAGTTTCTTAGCAGCAGTACAAGCTGAACTTACTGGTACGGGTGCAACTATAGCACCACCAGTAACTCAACCCCCATC
TTCAGATGGTATTGGTGTTGTTGAGATTCTTGTTCCAGAACTTAATGTAAGGGAATTTGCTAGCTTTGATTCACGAGTTG
TTAAGACAGTGAAAAAAGGTGAAATGTATCAAACTTGGGGACTATCTAATGGTCTATACAATGTTGGAGGAAACCAGTGG
GTGTCAGCAGGTCCTGCTTATGTTAAGTTTACTCCTGCAGGAAGTTCTAATGGAAATCCAGAAAATTTAGCTGGAAAACG
TGATCCAATTGGGAAAATTACAACTACTGCAAATCTAAACGTTCGTACACAATCTTCTACAGATGGGGATATTATTCGTA
CTATCCTTAATGGAGAAACGTGGAATATTTACGATATTAGTGATGGATGGGCGAGAGTGCATGATGGTTGGGTTTCTCTA
TCTTATGCAAATCTAACAAGATATTAA

Upstream 100 bases:

>100_bases
TTAGGAGCGCTCAATAAAGTAGATTAAGTTTAACCTTGAACTAAACATAGGAAGTGGTTAATTTAGTCGTGCATTTTAAT
CTATACGGAGGTGTACGACT

Downstream 100 bases:

>100_bases
TTTCCAAAAAAGTAGGCACATGTTGTGTCTGCTTTTTTATTTAGATAATTTAAAAGTTATTATTGACTTTATTTGATTAT
AGGGTTTTATTAATAATCCA

Product: N-acetylmuramoyl-L-alanine amidase XlyB

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MAIPVIQNLVAASKYPIKAPYSMNAEYITVHNTYNDAPAKNEINYMIGNNNEVSFHFAVDDIEVIQGIPVNRNAWHCGDG
GGNGNRKSIGVEICYSLSGGPRYEKAESLAIKFVAQLLRERGWGIDRVRTHKSWTEIGVRNGNSRAVKNCPHRILDEGRW
NSFLAAVQAELTGTGATIAPPVTQPPSSDGIGVVEILVPELNVREFASFDSRVVKTVKKGEMYQTWGLSNGLYNVGGNQW
VSAGPAYVKFTPAGSSNGNPENLAGKRDPIGKITTTANLNVRTQSSTDGDIIRTILNGETWNIYDISDGWARVHDGWVSL
SYANLTRY

Sequences:

>Translated_328_residues
MAIPVIQNLVAASKYPIKAPYSMNAEYITVHNTYNDAPAKNEINYMIGNNNEVSFHFAVDDIEVIQGIPVNRNAWHCGDG
GGNGNRKSIGVEICYSLSGGPRYEKAESLAIKFVAQLLRERGWGIDRVRTHKSWTEIGVRNGNSRAVKNCPHRILDEGRW
NSFLAAVQAELTGTGATIAPPVTQPPSSDGIGVVEILVPELNVREFASFDSRVVKTVKKGEMYQTWGLSNGLYNVGGNQW
VSAGPAYVKFTPAGSSNGNPENLAGKRDPIGKITTTANLNVRTQSSTDGDIIRTILNGETWNIYDISDGWARVHDGWVSL
SYANLTRY
>Mature_327_residues
AIPVIQNLVAASKYPIKAPYSMNAEYITVHNTYNDAPAKNEINYMIGNNNEVSFHFAVDDIEVIQGIPVNRNAWHCGDGG
GNGNRKSIGVEICYSLSGGPRYEKAESLAIKFVAQLLRERGWGIDRVRTHKSWTEIGVRNGNSRAVKNCPHRILDEGRWN
SFLAAVQAELTGTGATIAPPVTQPPSSDGIGVVEILVPELNVREFASFDSRVVKTVKKGEMYQTWGLSNGLYNVGGNQWV
SAGPAYVKFTPAGSSNGNPENLAGKRDPIGKITTTANLNVRTQSSTDGDIIRTILNGETWNIYDISDGWARVHDGWVSLS
YANLTRY

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 LysM repeat [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01510 Amidase_2; PF01476 LysM; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 35887; Mature: 35755

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPVIQNLVAASKYPIKAPYSMNAEYITVHNTYNDAPAKNEINYMIGNNNEVSFHFAVD
CCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEEEEC
DIEVIQGIPVNRNAWHCGDGGGNGNRKSIGVEICYSLSGGPRYEKAESLAIKFVAQLLRE
CCHHHCCCCCCCCCEECCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RGWGIDRVRTHKSWTEIGVRNGNSRAVKNCPHRILDEGRWNSFLAAVQAELTGTGATIAP
HCCCCCHHHHCCCHHHHEECCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCEECC
PVTQPPSSDGIGVVEILVPELNVREFASFDSRVVKTVKKGEMYQTWGLSNGLYNVGGNQW
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCE
VSAGPAYVKFTPAGSSNGNPENLAGKRDPIGKITTTANLNVRTQSSTDGDIIRTILNGET
EECCCCEEEEECCCCCCCCHHHCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCE
WNIYDISDGWARVHDGWVSLSYANLTRY
EEEEECCCCCEEEECCEEEEEECCCCCC
>Mature Secondary Structure 
AIPVIQNLVAASKYPIKAPYSMNAEYITVHNTYNDAPAKNEINYMIGNNNEVSFHFAVD
CCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEEEEC
DIEVIQGIPVNRNAWHCGDGGGNGNRKSIGVEICYSLSGGPRYEKAESLAIKFVAQLLRE
CCHHHCCCCCCCCCEECCCCCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RGWGIDRVRTHKSWTEIGVRNGNSRAVKNCPHRILDEGRWNSFLAAVQAELTGTGATIAP
HCCCCCHHHHCCCHHHHEECCCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCCEECC
PVTQPPSSDGIGVVEILVPELNVREFASFDSRVVKTVKKGEMYQTWGLSNGLYNVGGNQW
CCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCE
VSAGPAYVKFTPAGSSNGNPENLAGKRDPIGKITTTANLNVRTQSSTDGDIIRTILNGET
EECCCCEEEEECCCCCCCCHHHCCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHCCCE
WNIYDISDGWARVHDGWVSLSYANLTRY
EEEEECCCCCEEEECCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]