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The map label for this gene is yugJ [H]

Identifier: 218231360

GI number: 218231360

Start: 3730632

End: 3731795

Strand: Reverse

Name: yugJ [H]

Synonym: BCB4264_A3819

Alternate gene names: 218231360

Gene position: 3731795-3730632 (Counterclockwise)

Preceding gene: 218234980

Following gene: 218231971

Centisome position: 68.86

GC content: 35.48

Gene sequence:

>1164_bases
ATGCAAAATTTTGTATTTCGTAATCCAACGAAACTTATTTTCGGTAAAGGTCAATTAGAACAGTTAAAAACTGAAATTCC
ACAGTTCGGTAAAAAAGTTCTTCTCGTATACGGGGGAGGCAGCATTAAAAGAAACGGTATTTATGATAATATAATTTCTA
TTTTAAAGGATATTAATGTAGAAGTATTTGAATTAACAGGTGTTGAACCGAATCCACGTGTATCAACTGTAAAGAAAGGG
ATTCAAATTTGTAAAGAGAATGGTGTTGAATTTATTTTAGCAGTTGGTGGAGGAAGTGTAATCGACTGTACGAAGGCAAT
TGCAGCTGGTAGTAAGTACGATGGAGATGTATGGGATATTGTAACGAAAAAAACATTTGCTAATGAGGCATTACCATTTG
GTACTGTACTTACTCTTGCAGCAACAGGATCTGAAATGAATGCAGGCTCGGTAATTACAAACTGGGAAACGAATGAAAAG
TATGGATGGGGTAGCCCGGTTACTTTCCCGCAGTTTTCGATTTTAGATCCAGTTCATACTACTTCTGTACCGAAAGATCA
AACAATTTACGGTATGGTAGATATTATGTCGCACGTATTAGAACAATATTTCCATCATGGAACGAATACAGAACTACAAG
ATCGTTATTGTGAAGCTGTTTTAAAAACAGTAATTGAGACAGCTCCTAAGCTTTTAAGTGATTTAGAAAATTATGAGCAT
AGAGAAACTATTTTATATTGTGGTACTATGGCGTTAAACGGCATTTTAGCAATGGGAGTAAAAGGAGATTGGGCGACTCA
TAATATTGAGCATGCAGTTTCTGCTGTTCATGATATACCACACGGGGGTGGCCTTGCGATTTTATTCCCGAACTGGATGA
AGCATGTTGTAGAGGAAAATGTAAGTCGTTTTAAACAGTTTGCTATTCGAGTATTCAATGTAGAAACAGATGGAAAGACT
GATCGAGAAATTGCGTTAGAAGGAATTCAGGCGTTACGTCAATTTTGGACTTCAATTGAGGCGCCAGTAACATTATCTGA
TTACGCTATTGGAGAAAATGAAATTGATTTAATGGCGGATAAAGCGATGGCGTATGGTGAATTTGGTAACTTTAAAAAAT
TAAATAAAGATGATGTTTTAAGTATATATAAAGCTTCTTTATAA

Upstream 100 bases:

>100_bases
GGCTGGGATTTTTTTATTAAGCAAATATTTTTGTGAAATGAAAAAAATATGGTATACAAAAGTATAGTTGCTTTTCATAT
AGAAGGGATGGTAAAGAAAT

Downstream 100 bases:

>100_bases
GTGAATTTAGAAAAGAAACCTATTTGATTGTTTGATCGAATAGGTTTCTTTTTGCTGTTTATAAACGTTTTTGGATGTTT
TTGATTATTTTTGATTGTTT

Product: alcohol dehydrogenase, iron-containing

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MQNFVFRNPTKLIFGKGQLEQLKTEIPQFGKKVLLVYGGGSIKRNGIYDNIISILKDINVEVFELTGVEPNPRVSTVKKG
IQICKENGVEFILAVGGGSVIDCTKAIAAGSKYDGDVWDIVTKKTFANEALPFGTVLTLAATGSEMNAGSVITNWETNEK
YGWGSPVTFPQFSILDPVHTTSVPKDQTIYGMVDIMSHVLEQYFHHGTNTELQDRYCEAVLKTVIETAPKLLSDLENYEH
RETILYCGTMALNGILAMGVKGDWATHNIEHAVSAVHDIPHGGGLAILFPNWMKHVVEENVSRFKQFAIRVFNVETDGKT
DREIALEGIQALRQFWTSIEAPVTLSDYAIGENEIDLMADKAMAYGEFGNFKKLNKDDVLSIYKASL

Sequences:

>Translated_387_residues
MQNFVFRNPTKLIFGKGQLEQLKTEIPQFGKKVLLVYGGGSIKRNGIYDNIISILKDINVEVFELTGVEPNPRVSTVKKG
IQICKENGVEFILAVGGGSVIDCTKAIAAGSKYDGDVWDIVTKKTFANEALPFGTVLTLAATGSEMNAGSVITNWETNEK
YGWGSPVTFPQFSILDPVHTTSVPKDQTIYGMVDIMSHVLEQYFHHGTNTELQDRYCEAVLKTVIETAPKLLSDLENYEH
RETILYCGTMALNGILAMGVKGDWATHNIEHAVSAVHDIPHGGGLAILFPNWMKHVVEENVSRFKQFAIRVFNVETDGKT
DREIALEGIQALRQFWTSIEAPVTLSDYAIGENEIDLMADKAMAYGEFGNFKKLNKDDVLSIYKASL
>Mature_387_residues
MQNFVFRNPTKLIFGKGQLEQLKTEIPQFGKKVLLVYGGGSIKRNGIYDNIISILKDINVEVFELTGVEPNPRVSTVKKG
IQICKENGVEFILAVGGGSVIDCTKAIAAGSKYDGDVWDIVTKKTFANEALPFGTVLTLAATGSEMNAGSVITNWETNEK
YGWGSPVTFPQFSILDPVHTTSVPKDQTIYGMVDIMSHVLEQYFHHGTNTELQDRYCEAVLKTVIETAPKLLSDLENYEH
RETILYCGTMALNGILAMGVKGDWATHNIEHAVSAVHDIPHGGGLAILFPNWMKHVVEENVSRFKQFAIRVFNVETDGKT
DREIALEGIQALRQFWTSIEAPVTLSDYAIGENEIDLMADKAMAYGEFGNFKKLNKDDVLSIYKASL

Specific function: Unknown

COG id: COG1979

COG function: function code C; Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1789386, Length=391, Percent_Identity=38.618925831202, Blast_Score=275, Evalue=4e-75,
Organism=Escherichia coli, GI48994951, Length=392, Percent_Identity=25, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789163, Length=386, Percent_Identity=22.7979274611399, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787493, Length=316, Percent_Identity=25, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082107, Length=319, Percent_Identity=24.1379310344828, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=202, Percent_Identity=31.1881188118812, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321181, Length=387, Percent_Identity=24.8062015503876, Blast_Score=105, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 42847; Mature: 42847

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00913 ADH_IRON_1 ; PS00060 ADH_IRON_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNFVFRNPTKLIFGKGQLEQLKTEIPQFGKKVLLVYGGGSIKRNGIYDNIISILKDINV
CCCCCCCCCCEEEECCCHHHHHHHHHHHCCCEEEEEECCCCEECCCHHHHHHHHHHHCCE
EVFELTGVEPNPRVSTVKKGIQICKENGVEFILAVGGGSVIDCTKAIAAGSKYDGDVWDI
EEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
VTKKTFANEALPFGTVLTLAATGSEMNAGSVITNWETNEKYGWGSPVTFPQFSILDPVHT
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
TSVPKDQTIYGMVDIMSHVLEQYFHHGTNTELQDRYCEAVLKTVIETAPKLLSDLENYEH
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RETILYCGTMALNGILAMGVKGDWATHNIEHAVSAVHDIPHGGGLAILFPNWMKHVVEEN
HHHEEEEHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
VSRFKQFAIRVFNVETDGKTDREIALEGIQALRQFWTSIEAPVTLSDYAIGENEIDLMAD
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHH
KAMAYGEFGNFKKLNKDDVLSIYKASL
HHHHHCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQNFVFRNPTKLIFGKGQLEQLKTEIPQFGKKVLLVYGGGSIKRNGIYDNIISILKDINV
CCCCCCCCCCEEEECCCHHHHHHHHHHHCCCEEEEEECCCCEECCCHHHHHHHHHHHCCE
EVFELTGVEPNPRVSTVKKGIQICKENGVEFILAVGGGSVIDCTKAIAAGSKYDGDVWDI
EEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCCCCHHHH
VTKKTFANEALPFGTVLTLAATGSEMNAGSVITNWETNEKYGWGSPVTFPQFSILDPVHT
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
TSVPKDQTIYGMVDIMSHVLEQYFHHGTNTELQDRYCEAVLKTVIETAPKLLSDLENYEH
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
RETILYCGTMALNGILAMGVKGDWATHNIEHAVSAVHDIPHGGGLAILFPNWMKHVVEEN
HHHEEEEHHHHHCCHHEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHH
VSRFKQFAIRVFNVETDGKTDREIALEGIQALRQFWTSIEAPVTLSDYAIGENEIDLMAD
HHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHH
KAMAYGEFGNFKKLNKDDVLSIYKASL
HHHHHCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]