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The map label for this gene is nirB [H]

Identifier: 218231277

GI number: 218231277

Start: 2054466

End: 2056871

Strand: Reverse

Name: nirB [H]

Synonym: BCB4264_A2152

Alternate gene names: 218231277

Gene position: 2056871-2054466 (Counterclockwise)

Preceding gene: 218234750

Following gene: 218230850

Centisome position: 37.96

GC content: 38.36

Gene sequence:

>2406_bases
ATGAAAAAACGTTTAGTTATGATCGGAAATGGTATGGCTGGCATTCGCTGTATGGAAGAAATCTTAAAACATGATAGTGA
TTCATATGAGATTACTATTTTTGGAGATGAACCGCATCCAAACTACAACCGTATTATGCTCTCTCATGTTTTACAAGGCA
AAACAAATATGCAAGATATCATTATGAATGAATACAGTTGGTATGAAGAAAACGAAATAACTTTGTATACGAATGAAAGA
GTTCAAAGTATTAACCGAGAAGAAAAAATTATTATTACAGAAAAGAAGCGTACTCTTACATACGACAAGCTCATTATCGC
AACAGGTTCTAGTGCTTTTATTTTACCTGTTGAAGGTTCCGCACTTTCTGGTGTAACAGGATTTCGAACTATTGAAGATA
CACAATTTATGATAGATACCGCTAAAGAAAAGAAAAAGGCCGTTGTTATTGGTGGCGGTTTACTCGGTTTAGAAGCAGCA
CGAGGTCTAATTGATTTAGGAATGGACGTACATGTTGTTCATTTAATGCCTAGCTTAATGGAGCAACAACTAGATACGAA
AGCAGCTGCTCTTCTGCGTGAAGATTTAGAAGCACAAGGCATGAAATTTCTAATGGAAAAGAAAACTGTAAAAATTCTTG
GTACAGACCATGTTGAGGGCATTCAATTTCAAGACGGTGAAGTTGTAGATTGTGATTTAATCGTAATGGCTGTCGGAATA
CGCCCAAATACGCAAATAGCAAAAGATGCTGGTCTAATTGTAAATCGCGGTATTGTAGTCAATGACTATATGCTAACAAA
TGATGAGTCCATTTATGCAGTTGGAGAATGTGCAGAGCATGACGGTATCGCATATGGACTTGTTGCTCCTCTTTATGAAC
AAGGTGCGATATTAGCAAAACATATAACAAATTTACAAACTGATGGATATTCGGGCAGTATCGTCGGTACACAATTAAAA
GTTGCTGGTTGTGATTTATTCTCTGCTGGCCAAATTTATGAAGATGATCAAACGAAAGCAATATCCATCTTTAATGAATG
TAAACGTTCCTATAAAAAAGTATTAATTCGTGATAATAAAGTCGTCGGTATCGTTTTATACGGTGATACAGCTGACGGTA
CACGCCTCTTTAGCATGTTAAAGAAAGAAGAAGATATACAAGAATATACAGCCGCTTCTCTTCTTCACAAAGCTGGTGAA
GAATGTGAACTTGACGTTGCTACTATGAGTGCGAATGACACAATTTGCGGATGTAATGGTGTTACGAAAGGAACAATCGT
TCACGCCATTTTAGAACAAGAGTTAACGACTTTTGAAGAAGTTAAAGGCTGTACGAAAGCCGCAGGTTCTTGTGGTAAAT
GTCGTCCACTTGTGGAACAAGTTTTATCTCATACACTTGGAGATGCTTTTGATGCCTCAGCGCAATCTGCCGGTATGTGT
GGATGTACACCTTTATCCCGTGATGAAGTCGTAGCGGCCATTCACGAGAAAGGCTTAAAATCTCCAAAAGAAGTACGAAA
TGTTCTTGGTTTTGCACATGAAGACGGCTGTTCGAAATGCCGCCCTGCTTTAAACTACTATTTACGTATGGCGATTCCAG
AAGAATATGAAGATGATAAATCGTCCCGTTTCGTTAATGAAAGAATGAATGGTAACATCCAGCACGATGGTACATTCTCT
GTTATTCCACGTATGTACGGAGGCGTTACAACAGCTGATGATTTAATGAAAATTGCTGAAGTTGCGAAGAAGTATGATGT
TCCACTTGTGAAAATTACCGGTGCAAGCCGAATTGGCTTATACGGTGTCAAAAAACAAGATTTACCTAACGTATGGGCTG
ACTTAAATATGACTTCTGGATATGCGTATTCAAAGTCTCTTCGTAATGTAAAATCATGTGTTGGCTCTCGTTTTTGCCGT
TTCGGTACGAAAGATTCATTAGGACTTGGTATGTTACTTGAACAATCATTAGAAATGGTAGATACACCTCATAAAATGAA
AATGGGTGTAACCGGTTGTCCGCGTAACTGTGCGGAAGTACTGACGAAAGATCTTGGCGTTGTTTGTGTCGAAAATGGAT
ATCAACTTTATATTGGCGGAAATGGTGGTACAGAAGTACGTGAAGCTGATTTTGTAATGATTGTCCCTACTGAAGATGAT
GTCCTTCGCATCGCTGCAGCTTACATGCAATATTATCGTGAAACTGGTATTTACGGAGAGCGTACCGCCTACTGGACAGA
ACGTTTAGGCTTCGATCATATAAAAGAAATACTACAAGATGCAAGTATGGTTACTACGTTAAATGAACGTTTCCAAACAG
CTCGTAGAACATATACGGAAGCATGGGGACAAGCACTAGAAACGAAATCATTAAAAGCGATGTATGAAGTAGAAACTGTG
AAATAA

Upstream 100 bases:

>100_bases
TATAAAAATGTGATAAATATCACATCTCCCCCTTATTTGATTTTCTATAATAAAAATATAATCCTTTGTTCAACATTTCA
CATAAAAAGGGGAAACGAAA

Downstream 100 bases:

>100_bases
GGAGCTGTGATCTAAATGATACAAACGAAAGAAAAAATAAAAGTTATGCGTGCGGAAGATCTTGCTATTCAAATCGGTAA
AGAAGTGCAAATGAAAGGTA

Product: nitrite reductase [NAD(P)H], large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKKRLVMIGNGMAGIRCMEEILKHDSDSYEITIFGDEPHPNYNRIMLSHVLQGKTNMQDIIMNEYSWYEENEITLYTNER
VQSINREEKIIITEKKRTLTYDKLIIATGSSAFILPVEGSALSGVTGFRTIEDTQFMIDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAAALLREDLEAQGMKFLMEKKTVKILGTDHVEGIQFQDGEVVDCDLIVMAVGI
RPNTQIAKDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHITNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTAASLLHKAGE
ECELDVATMSANDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFAHEDGCSKCRPALNYYLRMAIPEEYEDDKSSRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDLGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLRIAAAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDASMVTTLNERFQTARRTYTEAWGQALETKSLKAMYEVETV
K

Sequences:

>Translated_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDSDSYEITIFGDEPHPNYNRIMLSHVLQGKTNMQDIIMNEYSWYEENEITLYTNER
VQSINREEKIIITEKKRTLTYDKLIIATGSSAFILPVEGSALSGVTGFRTIEDTQFMIDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAAALLREDLEAQGMKFLMEKKTVKILGTDHVEGIQFQDGEVVDCDLIVMAVGI
RPNTQIAKDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHITNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTAASLLHKAGE
ECELDVATMSANDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFAHEDGCSKCRPALNYYLRMAIPEEYEDDKSSRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDLGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLRIAAAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDASMVTTLNERFQTARRTYTEAWGQALETKSLKAMYEVETV
K
>Mature_801_residues
MKKRLVMIGNGMAGIRCMEEILKHDSDSYEITIFGDEPHPNYNRIMLSHVLQGKTNMQDIIMNEYSWYEENEITLYTNER
VQSINREEKIIITEKKRTLTYDKLIIATGSSAFILPVEGSALSGVTGFRTIEDTQFMIDTAKEKKKAVVIGGGLLGLEAA
RGLIDLGMDVHVVHLMPSLMEQQLDTKAAALLREDLEAQGMKFLMEKKTVKILGTDHVEGIQFQDGEVVDCDLIVMAVGI
RPNTQIAKDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHITNLQTDGYSGSIVGTQLK
VAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNKVVGIVLYGDTADGTRLFSMLKKEEDIQEYTAASLLHKAGE
ECELDVATMSANDTICGCNGVTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFAHEDGCSKCRPALNYYLRMAIPEEYEDDKSSRFVNERMNGNIQHDGTFS
VIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCR
FGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDLGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
VLRIAAAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDASMVTTLNERFQTARRTYTEAWGQALETKSLKAMYEVETV
K

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=276, Percent_Identity=26.4492753623188, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI21389617, Length=276, Percent_Identity=26.4492753623188, Blast_Score=97, Evalue=7e-20,
Organism=Homo sapiens, GI65787454, Length=276, Percent_Identity=26.4492753623188, Blast_Score=97, Evalue=7e-20,
Organism=Homo sapiens, GI22202629, Length=266, Percent_Identity=27.0676691729323, Blast_Score=67, Evalue=9e-11,
Organism=Escherichia coli, GI1789765, Length=814, Percent_Identity=35.1351351351351, Blast_Score=441, Evalue=1e-124,
Organism=Escherichia coli, GI1789065, Length=309, Percent_Identity=28.1553398058252, Blast_Score=134, Evalue=3e-32,
Organism=Escherichia coli, GI1788892, Length=280, Percent_Identity=24.6428571428571, Blast_Score=108, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=405, Percent_Identity=23.4567901234568, Blast_Score=103, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32564386, Length=372, Percent_Identity=24.1935483870968, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281359715, Length=281, Percent_Identity=23.1316725978648, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281359713, Length=281, Percent_Identity=23.1316725978648, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24639250, Length=281, Percent_Identity=23.1316725978648, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18543267, Length=281, Percent_Identity=23.1316725978648, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24639252, Length=281, Percent_Identity=23.1316725978648, Blast_Score=92, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24639257, Length=281, Percent_Identity=23.1316725978648, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585130, Length=347, Percent_Identity=22.1902017291066, Blast_Score=90, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88542; Mature: 88542

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDSDSYEITIFGDEPHPNYNRIMLSHVLQGKTNMQDI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
IMNEYSWYEENEITLYTNERVQSINREEKIIITEKKRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
ALSGVTGFRTIEDTQFMIDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECCCHHHHCCHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAAALLREDLEAQGMKFLMEKKTVKILGTDHVEGIQFQDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIAKDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCCHHHH
HITNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTAASLLHKAGEECELDVATMSANDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFAHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCCCCH
SSRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDLGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCH
VLRIAAAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDASMVTTLNERFQTARRTYTE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MKKRLVMIGNGMAGIRCMEEILKHDSDSYEITIFGDEPHPNYNRIMLSHVLQGKTNMQDI
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHH
IMNEYSWYEENEITLYTNERVQSINREEKIIITEKKRTLTYDKLIIATGSSAFILPVEGS
HHCCCCCCCCCCEEEEECHHHHHCCCCCEEEEEECCCEEEEEEEEEEECCCEEEEEECCC
ALSGVTGFRTIEDTQFMIDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLM
CCCCCCCCEEECCCHHHHCCHHCCCCEEEEECCHHHHHHHCHHHHCCCCEEHHHHHHHHH
EQQLDTKAAALLREDLEAQGMKFLMEKKTVKILGTDHVEGIQFQDGEVVDCDLIVMAVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCCEEEEEEEEEEECC
RPNTQIAKDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAK
CCCCCHHHCCCEEEECCEEEEEEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCCHHHH
HITNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKVLIRDNK
HHHHCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCHHHHHHHHHHHHHHHHEEEECCC
VVGIVLYGDTADGTRLFSMLKKEEDIQEYTAASLLHKAGEECELDVATMSANDTICGCNG
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEECCCC
VTKGTIVHAILEQELTTFEEVKGCTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSAGMC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHCCCCCCCCCC
GCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFAHEDGCSKCRPALNYYLRMAIPEEYEDDK
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHEEECCCCCCCCCH
SSRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCCCEECE
YGVKKQDLPNVWADLNMTSGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMV
ECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
DTPHKMKMGVTGCPRNCAEVLTKDLGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDD
CCCCHHCCCCCCCCHHHHHHHHHCCCEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCH
VLRIAAAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDASMVTTLNERFQTARRTYTE
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWGQALETKSLKAMYEVETVK
HHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]