Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 218231101

GI number: 218231101

Start: 3861360

End: 3862094

Strand: Reverse

Name: trmD [H]

Synonym: BCB4264_A3940

Alternate gene names: 218231101

Gene position: 3862094-3861360 (Counterclockwise)

Preceding gene: 218235147

Following gene: 218231872

Centisome position: 71.27

GC content: 40.82

Gene sequence:

>735_bases
ATGAAAATTGATATTTTAACGTTGTTCCCAGACATGTTTACCGGTGTATTTGGATCTTCTATATTAAAGAAGGCGCAAGA
AAAAGAAGCGGTAAACCTTCGTGTTGTTAACTTTCGTGATTATACAACAAGTAAGCATAATAGCGTAGACGATTATCCTT
ACGGCGGAGGAGCTGGTATGGTCCTTACTCCTCAGCCTATTTTTGATGCTGTAGAAGATTTGACGAAAGAGACAGAGCGT
AAGCCTAGAGTTGTCTTAATGTGTCCGCAGGGCGAAAGATTTACGCAGAAGAAAGCGGAAGAGCTTGCAGGGGAAGAACA
TTTGATCTTCGTATGTGGGCATTATGAAGGGTATGACGAACGAATTCGTGAGCATCTCGTAACGGATGAGATTTCTATTG
GTGACTATGTATTAACGGGCGGAGAATTAGCCTCTATGGTTATTACTGATAGTGTTGTACGTCTCTTGCCAGGTGTGCTA
GGAAATCATGCTTCACAAGTAGAGGATTCGTTTAGTACAGGTTTATTAGAGCATCCTCATTATACACGTCCAGCTGATTT
TCGTGGTATGAAGGTACCGGATGTATTAATGTCAGGGAATCATAAAAACATTGATGAATGGCGACATAAGGAATCTTTGC
GTCGAACGTATACGCGTAGACCTGATTTGTTAGATGAGAGAGAGTTATCTAAACAAGAGAAAAAATGGCTTGAGGAAATA
AAGCGAGAGCAGTAA

Upstream 100 bases:

>100_bases
AGGGTCAAGATCTCTTAATTCCTTATATCGATGACGTTGTACTTCAAGTTAATATTGAAAATAAATTAGTAACCATTCAT
GTAATGGAAGGGCTGCTATA

Downstream 100 bases:

>100_bases
TAACTTCATATATTTGCTATTGCAACAGGCCTAGTAATATGCTATTATAACATTTGTGCTACTGAAATCAGTAGCCGTAT
TAACGATGTTCCGCTGTAAT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVNLRVVNFRDYTTSKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETER
KPRVVLMCPQGERFTQKKAEELAGEEHLIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVL
GNHASQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLDERELSKQEKKWLEEI
KREQ

Sequences:

>Translated_244_residues
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVNLRVVNFRDYTTSKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETER
KPRVVLMCPQGERFTQKKAEELAGEEHLIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVL
GNHASQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLDERELSKQEKKWLEEI
KREQ
>Mature_244_residues
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVNLRVVNFRDYTTSKHNSVDDYPYGGGAGMVLTPQPIFDAVEDLTKETER
KPRVVLMCPQGERFTQKKAEELAGEEHLIFVCGHYEGYDERIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVL
GNHASQVEDSFSTGLLEHPHYTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLDERELSKQEKKWLEEI
KREQ

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=243, Percent_Identity=45.679012345679, Blast_Score=227, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27966; Mature: 27966

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVNLRVVNFRDYTTSKHNSVDDYPYGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCE
VLTPQPIFDAVEDLTKETERKPRVVLMCPQGERFTQKKAEELAGEEHLIFVCGHYEGYDE
EECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHH
RIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVLGNHASQVEDSFSTGLLEHPH
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
YTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLDERELSKQEKKWLEEI
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KREQ
HCCC
>Mature Secondary Structure
MKIDILTLFPDMFTGVFGSSILKKAQEKEAVNLRVVNFRDYTTSKHNSVDDYPYGGGAGM
CEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCE
VLTPQPIFDAVEDLTKETERKPRVVLMCPQGERFTQKKAEELAGEEHLIFVCGHYEGYDE
EECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEECCCCHHH
RIREHLVTDEISIGDYVLTGGELASMVITDSVVRLLPGVLGNHASQVEDSFSTGLLEHPH
HHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCC
YTRPADFRGMKVPDVLMSGNHKNIDEWRHKESLRRTYTRRPDLLDERELSKQEKKWLEEI
CCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
KREQ
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA