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The map label for this gene is aspA [H]

Identifier: 218231023

GI number: 218231023

Start: 633261

End: 634694

Strand: Direct

Name: aspA [H]

Synonym: BCB4264_A0647

Alternate gene names: 218231023

Gene position: 633261-634694 (Clockwise)

Preceding gene: 218233762

Following gene: 218231923

Centisome position: 11.69

GC content: 37.38

Gene sequence:

>1434_bases
ATGATAGCAACGAAGGATATACGTATAGAAAAAGATTTTTTAGGTGAAAAAGAAGTACCAAGTGTAGCTTATTATGGTGT
ACAAACATTACGTGCCGTAGAAAACTTCCCAATTACAGGATATCGCATTCATCCATCACTCATTACAGCAATGGCAATTG
TGAAAAAAGCGGCGGCGCTTGCGAATATAGATACTGGTTACTTAGCTAAAGACATTGGACATGAAATTGCAGAAGCAGCG
CAAGAAATTGTTGATGGAAAGTTTCATGATCAATTTATCGTGGACCCTATTCAAGGTGGAGCCGGAACTTCTATTAATAT
GAATACAAATGAAGTAATTGCGAATAGAGCGTTAGAACGTATGGGATATGAAAAAGGGGCGTATGCGAAAATTAGCCCAA
ACACTCATGTGAACATGGCCCAATCAACGAACGATGCGTTTCCAACAGGGATTCATATTGCAACTCTTATGATGTTAGAA
AAGCTTCTTATTACAATGGAAGAACTCCATTCTGCTTTTCGTAAAAAAGCAAAAGAGTTTGATCATGTTATTAAAATGGG
ACGCACACATTTACAAGATGCAGTTCCAATTCGCCTTGGGCAAGAATTTGAAGCGTATAGCCGAGTGCTTGCGCGTGATA
TAAAAAGAATTAAACAGTCTCGTCAGCATTTATATGAAGTGAATATGGGGGCGACAGCTGTTGGTACAGGATTGAATGCA
AATCCTATGTATATTGAACAAGTGGTAAAACATTTAAGAACGTTTAGTGGATTCCCACTTGTTGGTGCAGAGCATTTAGT
TGATGCAACGCAAAATACAGATGCATACACAGAAGTATCTGCAGCACTTAAAGTATGTATGATGAACATGTCTAAAATTG
CAAACGATCTTCGTATTATGGCGTCTGGACCACGTGTTGGGTTAGCTGAAATTCAATTGCCAGCACGTCAGCCAGGTTCA
TCGATTATGCCAGGAAAAGTAAATCCAGTTATGGCAGAAGTGATTAACCAAGTCGCATTCCAAGTAATCGGAAACGATCA
TACAATTTGCTTAGCATCAGAAGCAGGACAATTAGAGTTAAACGTAATGGAGCCTGTGCTTGTATTTAATTTAATTCAAT
CTATTAGTATTATGAATAATGGATTCCGTGTATTCCGTGAATATTGTATTGAAGGAATTACGGCAAATGAAGAATTGCTG
AAGCAATATGTTGAGAAAAGTGTTGGAATTATTACAGCAGTTAATCCTCATATTGGTTATGAAGTAGCATCTCGTATTGC
ACGTGAAGCAATTGAAACAGGGAAATCTGTTAGGGAGTTATGTTTAGAACATGGTGTACTGACAGAAGAGGAATTGGATA
TTATTTTAGATCCATTCGAAATGACTCACCCTGAAATTGCTGGAGCTTCTTTATTAAAAAATAAAAAAATGTAA

Upstream 100 bases:

>100_bases
TGAAGGTGGATACTGAAAAATTCTTCTGAATGATACTCGTGTTTTTAGAGGGCGGATTTTTTGGATAAGGATTTAGAATG
AAAAAGTATAGGTGATAAAA

Downstream 100 bases:

>100_bases
TGTGAAAAACACCGATCCACTTGGATCGGTGTTTTATTTTTAAAAGATATTAAATACAGCGTTTAATTGAAGTAAGCTAA
TAACAGCTAAGAAGATTAAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MIATKDIRIEKDFLGEKEVPSVAYYGVQTLRAVENFPITGYRIHPSLITAMAIVKKAAALANIDTGYLAKDIGHEIAEAA
QEIVDGKFHDQFIVDPIQGGAGTSINMNTNEVIANRALERMGYEKGAYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLE
KLLITMEELHSAFRKKAKEFDHVIKMGRTHLQDAVPIRLGQEFEAYSRVLARDIKRIKQSRQHLYEVNMGATAVGTGLNA
NPMYIEQVVKHLRTFSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGPRVGLAEIQLPARQPGS
SIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLIQSISIMNNGFRVFREYCIEGITANEELL
KQYVEKSVGIITAVNPHIGYEVASRIAREAIETGKSVRELCLEHGVLTEEELDIILDPFEMTHPEIAGASLLKNKKM

Sequences:

>Translated_477_residues
MIATKDIRIEKDFLGEKEVPSVAYYGVQTLRAVENFPITGYRIHPSLITAMAIVKKAAALANIDTGYLAKDIGHEIAEAA
QEIVDGKFHDQFIVDPIQGGAGTSINMNTNEVIANRALERMGYEKGAYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLE
KLLITMEELHSAFRKKAKEFDHVIKMGRTHLQDAVPIRLGQEFEAYSRVLARDIKRIKQSRQHLYEVNMGATAVGTGLNA
NPMYIEQVVKHLRTFSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGPRVGLAEIQLPARQPGS
SIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLIQSISIMNNGFRVFREYCIEGITANEELL
KQYVEKSVGIITAVNPHIGYEVASRIAREAIETGKSVRELCLEHGVLTEEELDIILDPFEMTHPEIAGASLLKNKKM
>Mature_477_residues
MIATKDIRIEKDFLGEKEVPSVAYYGVQTLRAVENFPITGYRIHPSLITAMAIVKKAAALANIDTGYLAKDIGHEIAEAA
QEIVDGKFHDQFIVDPIQGGAGTSINMNTNEVIANRALERMGYEKGAYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLE
KLLITMEELHSAFRKKAKEFDHVIKMGRTHLQDAVPIRLGQEFEAYSRVLARDIKRIKQSRQHLYEVNMGATAVGTGLNA
NPMYIEQVVKHLRTFSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIMASGPRVGLAEIQLPARQPGS
SIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLIQSISIMNNGFRVFREYCIEGITANEELL
KQYVEKSVGIITAVNPHIGYEVASRIAREAIETGKSVRELCLEHGVLTEEELDIILDPFEMTHPEIAGASLLKNKKM

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=468, Percent_Identity=42.7350427350427, Blast_Score=379, Evalue=1e-105,
Organism=Escherichia coli, GI87082375, Length=464, Percent_Identity=52.3706896551724, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1787896, Length=463, Percent_Identity=40.8207343412527, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1790398, Length=208, Percent_Identity=27.4038461538462, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=43.5064935064935, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565146, Length=328, Percent_Identity=41.4634146341463, Blast_Score=276, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6324993, Length=463, Percent_Identity=42.548596112311, Blast_Score=379, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640177, Length=468, Percent_Identity=41.2393162393162, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640179, Length=468, Percent_Identity=41.2393162393162, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI78710009, Length=464, Percent_Identity=42.0258620689655, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24662684, Length=471, Percent_Identity=40.7643312101911, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24583245, Length=465, Percent_Identity=36.1290322580645, Blast_Score=317, Evalue=9e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52613; Mature: 52613

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATKDIRIEKDFLGEKEVPSVAYYGVQTLRAVENFPITGYRIHPSLITAMAIVKKAAAL
CCCCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHH
ANIDTGYLAKDIGHEIAEAAQEIVDGKFHDQFIVDPIQGGAGTSINMNTNEVIANRALER
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEECCCHHHHHHHHHHHH
MGYEKGAYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLEKLLITMEELHSAFRKKAKEF
CCCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHVIKMGRTHLQDAVPIRLGQEFEAYSRVLARDIKRIKQSRQHLYEVNMGATAVGTGLNA
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
NPMYIEQVVKHLRTFSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIM
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
ASGPRVGLAEIQLPARQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLEL
ECCCCCCHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVLVFNLIQSISIMNNGFRVFREYCIEGITANEELLKQYVEKSVGIITAVNPHIGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCCH
EVASRIAREAIETGKSVRELCLEHGVLTEEELDIILDPFEMTHPEIAGASLLKNKKM
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIATKDIRIEKDFLGEKEVPSVAYYGVQTLRAVENFPITGYRIHPSLITAMAIVKKAAAL
CCCCCCCEECHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHHHH
ANIDTGYLAKDIGHEIAEAAQEIVDGKFHDQFIVDPIQGGAGTSINMNTNEVIANRALER
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEECCCHHHHHHHHHHHH
MGYEKGAYAKISPNTHVNMAQSTNDAFPTGIHIATLMMLEKLLITMEELHSAFRKKAKEF
CCCCCCCEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DHVIKMGRTHLQDAVPIRLGQEFEAYSRVLARDIKRIKQSRQHLYEVNMGATAVGTGLNA
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
NPMYIEQVVKHLRTFSGFPLVGAEHLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRIM
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
ASGPRVGLAEIQLPARQPGSSIMPGKVNPVMAEVINQVAFQVIGNDHTICLASEAGQLEL
ECCCCCCHHEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
NVMEPVLVFNLIQSISIMNNGFRVFREYCIEGITANEELLKQYVEKSVGIITAVNPHIGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEEECCCCCCH
EVASRIAREAIETGKSVRELCLEHGVLTEEELDIILDPFEMTHPEIAGASLLKNKKM
HHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]