Click here to switch to the map view.

The map label for this gene is pckA

Identifier: 21675041

GI number: 21675041

Start: 2095005

End: 2096870

Strand: Reverse

Name: pckA

Synonym: CT2232

Alternate gene names: 21675041

Gene position: 2096870-2095005 (Counterclockwise)

Preceding gene: 21675042

Following gene: 21675040

Centisome position: 97.3

GC content: 62.22

Gene sequence:

>1866_bases
ATGGAACCGATCCCGATCAATGCTCCGGACTCCGTCAGAAACCTGAAACTTCTCCAGTGGGTCAGGGAGACCGCCGAACT
GTGCCAGCCCGATTCGGTCTGCTGGTGTGACGGGTCCGTCGAGGAGTACGACCGCCTGTGCAACGAAATGGTCGCAAGCG
GCACCTTCATCAAGCTCTCCGAACAGAAGCGTCCCAACAGCTACCTCTGCCGTTCCGATCCGAGCGACGTGGCCAGGGTG
GAAGATCGCACCTTCATTTGCAGCATCCGCCGCCAGGACGCCGGACCGACCAACAACTGGGTCGCGCCAAAAGAGATGAA
GGCGACCTTGAACAAGCTCCTTGCCGGGTGCATGAAGGGGCGCACCATGTATATCATTCCCTTTAGCATGGGGCCGCTCG
GCTCGCACATCGCGCACATCGGCGTCGAGATCACCGACTCGCCCTACGTTGTCACCAACATGCGCATCATGACCCGCATG
GGCCGCGCGGTGCTCGACCTGCTCGACGAGGAGGCCGAGTTCGTGCCGTGCCTGCACTCGGTCGGCGCGCCGCTCGAACC
CGGTCAGCAGGATGTGCCGTGGCCCTGCAACGACACCAAGTACATCGTCCACTTCCCGGAGGAGCGCTCCATCGTCTCCT
ACGGCAGCGGCTACGGCGGCAACGCACTGCTCGGCAAGAAGTGCTTCGCCCTGCGCATTGCCTCCTCGATGGCGCGCGAC
GAGGGGTGGCTCGCCGAGCACATGCTCATTCTCGGCGTCGAGTCGCCGGAGGGCGAAAAAGATTACGTCGCGGCGGCCTT
CCCGAGCGCCTGCGGCAAAACCAACTTCGCCATGATGATTCCGCCGGGCGAAATGGAGGGGTGGAAAATCACCACCGTTG
GCGACGACATCGCCTGGATCAAGCAGGGCAAGGATGGTCGCCTCTACGCCATCAACCCCGAGTACGGCTTTTTCGGCGTC
GCGCCGGGCACCTCGGAGAAGAGCAACCCCAACGCGATGGCCACCCTTCACGCCAACTGCATCTTCACCAACGTGGCGCT
GACGCCCGATGGCGACGTCTGGTGGGAGGGGATGACCGACACGCCACCGGACTTCCTCATCGATTGGCAGGGCAAGCCCT
GGGTACCGGGGTGCGAACGTCCTGCCGCCCATCCGAACGCCCGCTTCACGGCTCCGGCCCATCAGTGCCCGGTTATCGAC
GAGAACTGGGAAAACCCGGACGGCGTGCCGATTTCAGCCTTCATTTTCGGTGGACGGCGTGGCGATACCATTCCGCTGGT
CTACCAGTCGGCCAACTGGTACTACGGCGTCTATCTTGCCGCCACGATGGGTTCGGAGAAGACCGCTGCCGCCGCAGGCA
AGATCGGCGACGTGCGCCGCGATCCTTTCGCCATGCTGCCTTTCTGTGGCTACCACATGGGTGACTACTTCAACCACTGG
CTGCACGTTGGCCGCACGCTTACCGATCCGCCGAGAATTTTCGGCGTCAACTGGTTCCGCAAGGATGAAAACGGCAAGTT
CCTTTGGCCCGGATTCGGCGAGAACATGCGCGTGCTGAAGTGGATTATTGGTCGGGTGCATGGCCGCGCCGCTGCGGTGG
AAAGCCCGCTCGGCTGGATGCCGAGGTATGAATCGCTCGACTGGCGGGGTCTGGATGGATTCACCAGAGACAAATTTTCA
ACGCTCATGTCGGTGGATCGCGAAGCGTGGAAGCAGGAGCTGTTCTCGCACGAAGAGCTGCTTGAAAAGCTTTACGACAG
GCTCCCGAAAGAGTTCACGCACATCCGCGAGCTGATGCTTTCGACGCTCTGGAGGTCTCCCGAGCACTGGGAGCTGGCCC
CCGAGCGCTATACGGCCGAGCACTGA

Upstream 100 bases:

>100_bases
CCGATGCCTCGGGGGAATTTCCTGCCGGGGCGTGCTTTTTTAACGCTATTTCTCTGCATCAAACATTTTAAACGATCCCC
AAGCGATCTCCAAGCTCATT

Downstream 100 bases:

>100_bases
GGATTGGCGCAGTTTCACAGACACTCCGAAAGCGGGCAGCGATGCCCGCTTTTTTTATTGGATTTCGACCCTATTTATCG
TCAGGTGTTGCACAATTGTT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MEPIPINAPDSVRNLKLLQWVRETAELCQPDSVCWCDGSVEEYDRLCNEMVASGTFIKLSEQKRPNSYLCRSDPSDVARV
EDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLLAGCMKGRTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRAVLDLLDEEAEFVPCLHSVGAPLEPGQQDVPWPCNDTKYIVHFPEERSIVSYGSGYGGNALLGKKCFALRIASSMARD
EGWLAEHMLILGVESPEGEKDYVAAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWIKQGKDGRLYAINPEYGFFGV
APGTSEKSNPNAMATLHANCIFTNVALTPDGDVWWEGMTDTPPDFLIDWQGKPWVPGCERPAAHPNARFTAPAHQCPVID
ENWENPDGVPISAFIFGGRRGDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAAAVESPLGWMPRYESLDWRGLDGFTRDKFS
TLMSVDREAWKQELFSHEELLEKLYDRLPKEFTHIRELMLSTLWRSPEHWELAPERYTAEH

Sequences:

>Translated_621_residues
MEPIPINAPDSVRNLKLLQWVRETAELCQPDSVCWCDGSVEEYDRLCNEMVASGTFIKLSEQKRPNSYLCRSDPSDVARV
EDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLLAGCMKGRTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRAVLDLLDEEAEFVPCLHSVGAPLEPGQQDVPWPCNDTKYIVHFPEERSIVSYGSGYGGNALLGKKCFALRIASSMARD
EGWLAEHMLILGVESPEGEKDYVAAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWIKQGKDGRLYAINPEYGFFGV
APGTSEKSNPNAMATLHANCIFTNVALTPDGDVWWEGMTDTPPDFLIDWQGKPWVPGCERPAAHPNARFTAPAHQCPVID
ENWENPDGVPISAFIFGGRRGDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAAAVESPLGWMPRYESLDWRGLDGFTRDKFS
TLMSVDREAWKQELFSHEELLEKLYDRLPKEFTHIRELMLSTLWRSPEHWELAPERYTAEH
>Mature_621_residues
MEPIPINAPDSVRNLKLLQWVRETAELCQPDSVCWCDGSVEEYDRLCNEMVASGTFIKLSEQKRPNSYLCRSDPSDVARV
EDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLLAGCMKGRTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRM
GRAVLDLLDEEAEFVPCLHSVGAPLEPGQQDVPWPCNDTKYIVHFPEERSIVSYGSGYGGNALLGKKCFALRIASSMARD
EGWLAEHMLILGVESPEGEKDYVAAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWIKQGKDGRLYAINPEYGFFGV
APGTSEKSNPNAMATLHANCIFTNVALTPDGDVWWEGMTDTPPDFLIDWQGKPWVPGCERPAAHPNARFTAPAHQCPVID
ENWENPDGVPISAFIFGGRRGDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAAAVESPLGWMPRYESLDWRGLDGFTRDKFS
TLMSVDREAWKQELFSHEELLEKLYDRLPKEFTHIRELMLSTLWRSPEHWELAPERYTAEH

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI66346721, Length=587, Percent_Identity=52.9812606473594, Blast_Score=600, Evalue=1e-172,
Organism=Homo sapiens, GI187281517, Length=588, Percent_Identity=51.530612244898, Blast_Score=593, Evalue=1e-169,
Organism=Homo sapiens, GI66346723, Length=371, Percent_Identity=54.7169811320755, Blast_Score=393, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71989645, Length=592, Percent_Identity=49.6621621621622, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI71989658, Length=592, Percent_Identity=49.6621621621622, Blast_Score=564, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17555492, Length=593, Percent_Identity=49.0725126475548, Blast_Score=557, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71989653, Length=579, Percent_Identity=49.9136442141624, Blast_Score=553, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI32564411, Length=537, Percent_Identity=51.7690875232775, Blast_Score=537, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI32564407, Length=501, Percent_Identity=51.6966067864271, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71989649, Length=494, Percent_Identity=51.0121457489879, Blast_Score=490, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI32564409, Length=346, Percent_Identity=49.7109826589595, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17540956, Length=613, Percent_Identity=24.1435562805873, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24655085, Length=585, Percent_Identity=53.1623931623932, Blast_Score=603, Evalue=1e-172,
Organism=Drosophila melanogaster, GI24655082, Length=579, Percent_Identity=52.5043177892919, Blast_Score=583, Evalue=1e-167,
Organism=Drosophila melanogaster, GI161077226, Length=548, Percent_Identity=53.1021897810219, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24655088, Length=474, Percent_Identity=56.1181434599156, Blast_Score=528, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_CHLTE (Q8KAD1)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_663106.1
- ProteinModelPortal:   Q8KAD1
- SMR:   Q8KAD1
- GeneID:   1007328
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2232
- NMPDR:   fig|194439.1.peg.2200
- TIGR:   CT2232
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BioCyc:   CTEP194439:CT_2232-MONOMER
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 69933; Mature: 69933

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 271-271 BINDING 79-79 BINDING 220-220 BINDING 227-227 BINDING 269-269 BINDING 388-388 BINDING 419-419

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIPINAPDSVRNLKLLQWVRETAELCQPDSVCWCDGSVEEYDRLCNEMVASGTFIKLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEE
EQKRPNSYLCRSDPSDVARVEDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLLAGCMKG
CCCCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRMGRAVLDLLDEEAEFVPCLHS
CEEEEEEECCCCCHHHHEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAPLEPGQQDVPWPCNDTKYIVHFPEERSIVSYGSGYGGNALLGKKCFALRIASSMARD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
EGWLAEHMLILGVESPEGEKDYVAAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWI
CCHHEEEEEEEEECCCCCCCCEEEEECCHHCCCCCEEEEECCCCCCCEEEEEECCCCHHH
KQGKDGRLYAINPEYGFFGVAPGTSEKSNPNAMATLHANCIFTNVALTPDGDVWWEGMTD
CCCCCCCEEEECCCCCEEECCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCEEECCCCC
TPPDFLIDWQGKPWVPGCERPAAHPNARFTAPAHQCPVIDENWENPDGVPISAFIFGGRR
CCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
GDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
CCEEEEEEECCCEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHH
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAAAVESPLGWM
HHHCCCCCCCCCEEECEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCC
PRYESLDWRGLDGFTRDKFSTLMSVDREAWKQELFSHEELLEKLYDRLPKEFTHIRELML
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
STLWRSPEHWELAPERYTAEH
HHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEPIPINAPDSVRNLKLLQWVRETAELCQPDSVCWCDGSVEEYDRLCNEMVASGTFIKLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEE
EQKRPNSYLCRSDPSDVARVEDRTFICSIRRQDAGPTNNWVAPKEMKATLNKLLAGCMKG
CCCCCCCCCCCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RTMYIIPFSMGPLGSHIAHIGVEITDSPYVVTNMRIMTRMGRAVLDLLDEEAEFVPCLHS
CEEEEEEECCCCCHHHHEEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAPLEPGQQDVPWPCNDTKYIVHFPEERSIVSYGSGYGGNALLGKKCFALRIASSMARD
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHC
EGWLAEHMLILGVESPEGEKDYVAAAFPSACGKTNFAMMIPPGEMEGWKITTVGDDIAWI
CCHHEEEEEEEEECCCCCCCCEEEEECCHHCCCCCEEEEECCCCCCCEEEEEECCCCHHH
KQGKDGRLYAINPEYGFFGVAPGTSEKSNPNAMATLHANCIFTNVALTPDGDVWWEGMTD
CCCCCCCEEEECCCCCEEECCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCCEEECCCCC
TPPDFLIDWQGKPWVPGCERPAAHPNARFTAPAHQCPVIDENWENPDGVPISAFIFGGRR
CCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
GDTIPLVYQSANWYYGVYLAATMGSEKTAAAAGKIGDVRRDPFAMLPFCGYHMGDYFNHW
CCEEEEEEECCCEEEEEEEEEECCCCHHHHHHCCCCCCCCCCCEECCHHHHHHHHHHHHH
LHVGRTLTDPPRIFGVNWFRKDENGKFLWPGFGENMRVLKWIIGRVHGRAAAVESPLGWM
HHHCCCCCCCCCEEECEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCC
PRYESLDWRGLDGFTRDKFSTLMSVDREAWKQELFSHEELLEKLYDRLPKEFTHIRELML
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
STLWRSPEHWELAPERYTAEH
HHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901