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The map label for this gene is gidB

Identifier: 21674969

GI number: 21674969

Start: 2048509

End: 2049162

Strand: Direct

Name: gidB

Synonym: CT2160

Alternate gene names: 21674969

Gene position: 2048509-2049162 (Clockwise)

Preceding gene: 21674967

Following gene: 21675004

Centisome position: 95.06

GC content: 56.12

Gene sequence:

>654_bases
ATGCAGAACGACCTGCACCTACTCGAGGGACTCTGCCGTCAGCACGGCATCCCGGTTACCAAAAATGCTCTTACACTTCT
GGTGCGCTATGCCAGGCTTCTCGAAGCCTGGAACCTCAAGGTAAACCTCGTCAGCCGCAAGGAACATGCGCCGGTCATCG
TCAAGCATGTGTTTCACTCGCTGCTCATCGCCCGCATCCACGACTTCAAACCCGGCGAAACGGTGCTCGACCTCGGCACC
GGCGGCGGCTTGCCAGGCATTCCGCTGGCCATCCTTTTTCCTGAAACCTCATTCCTGCTGGTCGATTCCACCGGCAAGAA
GATCGCTGCCTGCAAAGCAATGATCAAGGAACTTGGCCTCGAAAACGTCATTGCCCTGCATTCAAGAGTCGAAGAACTGA
AAGGTGTGATCTTTGACACGGTGCTCAGCCGTCAGGTTGCACCGCTCGAAGAGTTATGCGCCTACAGCGCCAGGCTGCTC
AAACACGATGGGGTGCTCATCTGCCTCAAAGGCGGCAGCCTCAATGAAGAGATTGCCGAAGCGGTGCTGTCAAGGGAGAA
GCATCTGGGATTCCCCGCCTCAGTGGATCAGCTTCCGATAGGAGAGATCGATCCGATGTTCTCAGAAAAGCAGATAGTCA
TCGCCCGCTGGTGA

Upstream 100 bases:

>100_bases
GGCATTTCCCCGGGGCACAGAACATTTGCCATTGGCGTTAAAATGTCGTTTTTTCCCTGTTGTATGCTTTTTCATCACCA
TCGCAGCCGAACTATTCGTC

Downstream 100 bases:

>100_bases
TCGAGAAGATCGAGCGAACGATGACTACCGTGTTTTTTCTACAATCCGAGAAGATGTCGCCTTTATTCAGCGGCGTCACC
ACCCTCGTTCTCCTGAGATC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MQNDLHLLEGLCRQHGIPVTKNALTLLVRYARLLEAWNLKVNLVSRKEHAPVIVKHVFHSLLIARIHDFKPGETVLDLGT
GGGLPGIPLAILFPETSFLLVDSTGKKIAACKAMIKELGLENVIALHSRVEELKGVIFDTVLSRQVAPLEELCAYSARLL
KHDGVLICLKGGSLNEEIAEAVLSREKHLGFPASVDQLPIGEIDPMFSEKQIVIARW

Sequences:

>Translated_217_residues
MQNDLHLLEGLCRQHGIPVTKNALTLLVRYARLLEAWNLKVNLVSRKEHAPVIVKHVFHSLLIARIHDFKPGETVLDLGT
GGGLPGIPLAILFPETSFLLVDSTGKKIAACKAMIKELGLENVIALHSRVEELKGVIFDTVLSRQVAPLEELCAYSARLL
KHDGVLICLKGGSLNEEIAEAVLSREKHLGFPASVDQLPIGEIDPMFSEKQIVIARW
>Mature_217_residues
MQNDLHLLEGLCRQHGIPVTKNALTLLVRYARLLEAWNLKVNLVSRKEHAPVIVKHVFHSLLIARIHDFKPGETVLDLGT
GGGLPGIPLAILFPETSFLLVDSTGKKIAACKAMIKELGLENVIALHSRVEELKGVIFDTVLSRQVAPLEELCAYSARLL
KHDGVLICLKGGSLNEEIAEAVLSREKHLGFPASVDQLPIGEIDPMFSEKQIVIARW

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=176, Percent_Identity=36.9318181818182, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_CHLTE (Q8KAK0)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_663034.1
- ProteinModelPortal:   Q8KAK0
- SMR:   Q8KAK0
- GeneID:   1007399
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2160
- NMPDR:   fig|194439.1.peg.2128
- TIGR:   CT2160
- HOGENOM:   HBG308018
- OMA:   YELLVEW
- ProtClustDB:   PRK00107
- BioCyc:   CTEP194439:CT_2160-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 23899; Mature: 23899

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNDLHLLEGLCRQHGIPVTKNALTLLVRYARLLEAWNLKVNLVSRKEHAPVIVKHVFHS
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHH
LLIARIHDFKPGETVLDLGTGGGLPGIPLAILFPETSFLLVDSTGKKIAACKAMIKELGL
HHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCH
ENVIALHSRVEELKGVIFDTVLSRQVAPLEELCAYSARLLKHDGVLICLKGGSLNEEIAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
AVLSREKHLGFPASVDQLPIGEIDPMFSEKQIVIARW
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MQNDLHLLEGLCRQHGIPVTKNALTLLVRYARLLEAWNLKVNLVSRKEHAPVIVKHVFHS
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHH
LLIARIHDFKPGETVLDLGTGGGLPGIPLAILFPETSFLLVDSTGKKIAACKAMIKELGL
HHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCH
ENVIALHSRVEELKGVIFDTVLSRQVAPLEELCAYSARLLKHDGVLICLKGGSLNEEIAE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHH
AVLSREKHLGFPASVDQLPIGEIDPMFSEKQIVIARW
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901