Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 21674967

GI number: 21674967

Start: 2045675

End: 2047138

Strand: Direct

Name: cysS

Synonym: CT2158

Alternate gene names: 21674967

Gene position: 2045675-2047138 (Clockwise)

Preceding gene: 21674965

Following gene: 21674969

Centisome position: 94.93

GC content: 59.63

Gene sequence:

>1464_bases
ATGTCCTCTCTGCATATCTACAACTCGCTTACCCGCACAAAGGAACCCTTTAAACCCATCCATCCCGGTCTGGCGACTAT
CTACGTTTGCGGCCCGACCGTGTACGGCCACGCTCACCTCGGCCACGCCAAAAGCTACGTATCATTCGACGTGGTGGTGC
GCTGGCTGCGTCATGTCGGCGAAGAGCAGGGCTACAAGGTTCGGTACGTGCAGAACATCACCGACGTAGGCCACCTGACC
GACGACGCCGACGAAGGGGAGGACAAAATCCAGAAGCAGGCTCGGCAGGAGCGCATCGAACCGATGGAGGTGGCGCAGTA
CTACACCCGCAGCTTCTACGAGGATATGGACCGGCTTGGCGTCGAGCGCCCGAACATCGCGCCGACCGCGACCGGGCATA
TCCCGGAGCAGATCGCGCTGGTCGAGCGGCTCATCGAAAGCGGCCACGCCTACGAATCCAACGGCAACGTGTATTTTGAC
GTCAACTCGTTCGAGGGGTACGGCAAGCTTTCGGGCCGTACCGACCAGGAGGCGTTGCAATCGGGCGGGCGCGTCGCAGA
GCGAAGCGACAAGCGTAACCCTTCGGACTTCGCACTCTGGAAAAAGGCCGAGCCGGGCCACATCATGAAATGGCAGTCGC
CCTGGGGCGAGGGCTATCCAGGCTGGCATCTGGAGTGTTCGGCGATGGCGATGAAGTACCTCGGCGACACCATCGACATC
CACGGTGGCGGCATGGAGAACAAGTTCCCGCACCACGACTGCGAGATCGCGCAGTCCGAAGCGGCCACCGGCAAGCCCTT
CGTGCGCTACTGGATGCACAACAACATGGTGACGGTCGATGGCGTCAAGATGGGCAAGTCGCTCAAGAACTTCGTGAACC
TCAAGGAGCTGTTCGGGAAATTCGACCCGCTGGTCATCCGCTTCTTCATTTTGCAATCGCACTACCGCTCCCCGCTCGAC
TTCTCCGAAGCGGCCATCAGGGCATCGCAGTCAGGTTTCGAAAAGTTGCAGGAAACGTACAAGCGATTGGTAGAGTCGGC
GGAGGGCAAGGGCCAGCTCGACGTGGCAACGTTCGAGCAGAAAATCACCGACGCGCTCAACGACGACTTCAACACACCGG
TCGCCATTGCCGTGCTTTTCGAGTTCATCAAAGCGCTCAACGGCGCGCTCGACAAAGACGGGCTTGACGCCGCATCAAAA
TCTGGCGCTCAGAACCTTTTCGATAGCTACGCCGGAAAAGTCCTCGGCATTCTGAAAAGCCGCGACGAACTGCTGGCCGG
AGAGAGCGGCGAAAGCGCGCAAACCCTCAACGACGTCATGGCTGTGCTGCTCGAACTCCGCAAGGAGGCTCGCGCCAGCA
AGGATTTCGCCACCAGCGACAAAATCCGCGACCTGCTCATGGAGCGCGGCATCGAAATCAAGGACACCAGGGAAGGCGCA
ACGTGGTCAAAGAAAAAAGCCTGA

Upstream 100 bases:

>100_bases
GTGGCCGCTGAAAAGCCTTGCCTTACCGAGCCAAATGTTTAACTTTTAAAACATTTTCCGGTCATAAGCCGGGATTCGGC
AACTCTTCATCGCTATCCGG

Downstream 100 bases:

>100_bases
AAAAAAGAAAGCCGGAGGTTGTGCTCCGGCTTTCTTTTTTTCTCTGACCACCCCACATCATCCTACAGGAACCCCTCCAA
CCCCGGCTCCCGCCTCATCA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSSLHIYNSLTRTKEPFKPIHPGLATIYVCGPTVYGHAHLGHAKSYVSFDVVVRWLRHVGEEQGYKVRYVQNITDVGHLT
DDADEGEDKIQKQARQERIEPMEVAQYYTRSFYEDMDRLGVERPNIAPTATGHIPEQIALVERLIESGHAYESNGNVYFD
VNSFEGYGKLSGRTDQEALQSGGRVAERSDKRNPSDFALWKKAEPGHIMKWQSPWGEGYPGWHLECSAMAMKYLGDTIDI
HGGGMENKFPHHDCEIAQSEAATGKPFVRYWMHNNMVTVDGVKMGKSLKNFVNLKELFGKFDPLVIRFFILQSHYRSPLD
FSEAAIRASQSGFEKLQETYKRLVESAEGKGQLDVATFEQKITDALNDDFNTPVAIAVLFEFIKALNGALDKDGLDAASK
SGAQNLFDSYAGKVLGILKSRDELLAGESGESAQTLNDVMAVLLELRKEARASKDFATSDKIRDLLMERGIEIKDTREGA
TWSKKKA

Sequences:

>Translated_487_residues
MSSLHIYNSLTRTKEPFKPIHPGLATIYVCGPTVYGHAHLGHAKSYVSFDVVVRWLRHVGEEQGYKVRYVQNITDVGHLT
DDADEGEDKIQKQARQERIEPMEVAQYYTRSFYEDMDRLGVERPNIAPTATGHIPEQIALVERLIESGHAYESNGNVYFD
VNSFEGYGKLSGRTDQEALQSGGRVAERSDKRNPSDFALWKKAEPGHIMKWQSPWGEGYPGWHLECSAMAMKYLGDTIDI
HGGGMENKFPHHDCEIAQSEAATGKPFVRYWMHNNMVTVDGVKMGKSLKNFVNLKELFGKFDPLVIRFFILQSHYRSPLD
FSEAAIRASQSGFEKLQETYKRLVESAEGKGQLDVATFEQKITDALNDDFNTPVAIAVLFEFIKALNGALDKDGLDAASK
SGAQNLFDSYAGKVLGILKSRDELLAGESGESAQTLNDVMAVLLELRKEARASKDFATSDKIRDLLMERGIEIKDTREGA
TWSKKKA
>Mature_486_residues
SSLHIYNSLTRTKEPFKPIHPGLATIYVCGPTVYGHAHLGHAKSYVSFDVVVRWLRHVGEEQGYKVRYVQNITDVGHLTD
DADEGEDKIQKQARQERIEPMEVAQYYTRSFYEDMDRLGVERPNIAPTATGHIPEQIALVERLIESGHAYESNGNVYFDV
NSFEGYGKLSGRTDQEALQSGGRVAERSDKRNPSDFALWKKAEPGHIMKWQSPWGEGYPGWHLECSAMAMKYLGDTIDIH
GGGMENKFPHHDCEIAQSEAATGKPFVRYWMHNNMVTVDGVKMGKSLKNFVNLKELFGKFDPLVIRFFILQSHYRSPLDF
SEAAIRASQSGFEKLQETYKRLVESAEGKGQLDVATFEQKITDALNDDFNTPVAIAVLFEFIKALNGALDKDGLDAASKS
GAQNLFDSYAGKVLGILKSRDELLAGESGESAQTLNDVMAVLLELRKEARASKDFATSDKIRDLLMERGIEIKDTREGAT
WSKKKA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=512, Percent_Identity=36.9140625, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI21269877, Length=421, Percent_Identity=32.3040380047506, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI303304987, Length=421, Percent_Identity=32.3040380047506, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI62240992, Length=421, Percent_Identity=32.3040380047506, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI10835051, Length=421, Percent_Identity=32.3040380047506, Blast_Score=196, Evalue=5e-50,
Organism=Escherichia coli, GI1786737, Length=486, Percent_Identity=41.7695473251029, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17509795, Length=263, Percent_Identity=36.8821292775665, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17509797, Length=263, Percent_Identity=36.8821292775665, Blast_Score=174, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6324082, Length=413, Percent_Identity=32.6876513317191, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI19922218, Length=519, Percent_Identity=32.1772639691715, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19922364, Length=315, Percent_Identity=35.8730158730159, Blast_Score=192, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_CHLTE (Q8KAK2)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_663032.1
- ProteinModelPortal:   Q8KAK2
- GeneID:   1007401
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2158
- NMPDR:   fig|194439.1.peg.2126
- TIGR:   CT2158
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   CTEP194439:CT_2158-MONOMER
- BRENDA:   6.1.1.16
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54601; Mature: 54470

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 286-286

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLHIYNSLTRTKEPFKPIHPGLATIYVCGPTVYGHAHLGHAKSYVSFDVVVRWLRHVG
CCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCEECCCHHCHHHHHHHHHHHHHHHHHHC
EEQGYKVRYVQNITDVGHLTDDADEGEDKIQKQARQERIEPMEVAQYYTRSFYEDMDRLG
CCCCCEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
VERPNIAPTATGHIPEQIALVERLIESGHAYESNGNVYFDVNSFEGYGKLSGRTDQEALQ
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHH
SGGRVAERSDKRNPSDFALWKKAEPGHIMKWQSPWGEGYPGWHLECSAMAMKYLGDTIDI
CCCCCHHHCCCCCCCHHHEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEE
HGGGMENKFPHHDCEIAQSEAATGKPFVRYWMHNNMVTVDGVKMGKSLKNFVNLKELFGK
CCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCC
FDPLVIRFFILQSHYRSPLDFSEAAIRASQSGFEKLQETYKRLVESAEGKGQLDVATFEQ
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
KITDALNDDFNTPVAIAVLFEFIKALNGALDKDGLDAASKSGAQNLFDSYAGKVLGILKS
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDELLAGESGESAQTLNDVMAVLLELRKEARASKDFATSDKIRDLLMERGIEIKDTREGA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCCC
TWSKKKA
CCCCCCC
>Mature Secondary Structure 
SSLHIYNSLTRTKEPFKPIHPGLATIYVCGPTVYGHAHLGHAKSYVSFDVVVRWLRHVG
CCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCEECCCHHCHHHHHHHHHHHHHHHHHHC
EEQGYKVRYVQNITDVGHLTDDADEGEDKIQKQARQERIEPMEVAQYYTRSFYEDMDRLG
CCCCCEEEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
VERPNIAPTATGHIPEQIALVERLIESGHAYESNGNVYFDVNSFEGYGKLSGRTDQEALQ
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHH
SGGRVAERSDKRNPSDFALWKKAEPGHIMKWQSPWGEGYPGWHLECSAMAMKYLGDTIDI
CCCCCHHHCCCCCCCHHHEEECCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCEEEE
HGGGMENKFPHHDCEIAQSEAATGKPFVRYWMHNNMVTVDGVKMGKSLKNFVNLKELFGK
CCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHCC
FDPLVIRFFILQSHYRSPLDFSEAAIRASQSGFEKLQETYKRLVESAEGKGQLDVATFEQ
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
KITDALNDDFNTPVAIAVLFEFIKALNGALDKDGLDAASKSGAQNLFDSYAGKVLGILKS
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RDELLAGESGESAQTLNDVMAVLLELRKEARASKDFATSDKIRDLLMERGIEIKDTREGA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEECCCCC
TWSKKKA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901