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The map label for this gene is hydA [H]

Identifier: 21674706

GI number: 21674706

Start: 1793217

End: 1794491

Strand: Direct

Name: hydA [H]

Synonym: CT1894

Alternate gene names: 21674706

Gene position: 1793217-1794491 (Clockwise)

Preceding gene: 21674705

Following gene: 21674712

Centisome position: 83.21

GC content: 62.82

Gene sequence:

>1275_bases
ATGAAGGTTGACTTCAACATAGACATCCACCACCTGCCGAGGGTCGAAGGCCACGGCGATATTCGCATTTCAGTCAGGGA
CGGCAAGCTCGTTGACGCCAAATGGGCGGTGGTCGAAACGCCGCGCTTTTTCGAGGTGATGGTGAAGGGACTCTCCGCCG
AGCGGGTGCCGTTCCTCACCTCGCGCATCTGCGGCATCTGCTCGATCAGCCACTCGCTGGCCAGCATCCGCTCGCTCGAA
CGCGCCATGCAGATCACGCCGCCCGAAACGGCGCGAATCATCCGCCTGCTCGCCATGCACGGCGAGACGCTGCAAAGCCA
CGCGTTGCACCTCTTCTTCCTCGCCGCGCCGGACTTCATGGGCACGCCGAGCGTCGTGCCGCTCATGCAATCGCACCCGG
AGGTGGTCGAGGCGGGCCTCTTGCTCAAGGAGCTGGGCAACGAACTGAGCATCGCCACCACGGGCCGCGCAACGCATCCG
GTCAGCCTCGTGCTCGGCGGCGTCAGCAAAGCCCCCGCGAAGCAGCGTTTGGCGGAGATCAAACAGATGATCGCCGACCG
CAAGCCGATGCTCGACCGGGCCACCGACTTTTTCATGACGCTCCGCGTGCCGGAGTTCGTGCGTGAAACCGAGTTCATCT
CACTGCACGACGGCAAAAGCTACCCCTACATCGGCGGCAACCTTGTTTCGACCGACGGCGTGAAGCGCGACGAGAACGAC
TATCTGGCGATGACCAACGAGTACACGGTGGATTTTTCGACCTCGAAGTTCACAAGGCTCAGCCGCGAGTCCTCAGCCGC
CGGAGCGCTGGCGCGGTTCAACAACAACTACGCCCAGCTCCATCCGCGAGCCAAAGAGGCAGCGGCGGCGTTCGGCCTCG
AACCGGTCTGTCACAACCCGTTCATGAACAACATCGCCCAGCTCGTCGAGTGTCACCACCTCGTGGCCGACGCCGAAGAG
CTCATCGACCGCCTGCTCGACGACGACCTGCGCAACATCAAGGCCGACTACAAGCCGCGAGCCGGAGCCGCCACCGGAGC
CGTCGAAGCGCCGCGCGGCGTGCTCTACCACTACATGGAGACCGACGAGAGCGGCAAGGTGGTCAAAGGCGACTGCATCA
TCCCCACAACGCAGAACAACGCCAACATCCACTACGACCTCCACGCCCTCGCCGAGCAATCGCTGGCGCAAGGCATGGGC
GAAAAAGAGGTTGAAAAGCTCTGCGAAATGCTGGTGCGCTCCTACGACCCCTGCATTTCATGCTCGGTGCACTGA

Upstream 100 bases:

>100_bases
TACGACTCGTTCGTGCAGCTCGTTAAAGAGCACGGACTGAGCGAGAACGAAATGAACGAAAAGCTCGCATTTTACAACGG
TTTCGCAGAGTATCTGAGCC

Downstream 100 bases:

>100_bases
GCATCTTTAAGCCACGTTCTCAATGACAAAAGACCCGAAAAATCGGGGCTTTTGCTTTGGTATTTCATTCTCCTGCCGGG
CAGCAGGGGCTCTGGCAACT

Product: hydrogenase/sulfur reductase, alpha subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Methyl viologen-reducing hydrogenase subunit alpha; MVH subunit A [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MKVDFNIDIHHLPRVEGHGDIRISVRDGKLVDAKWAVVETPRFFEVMVKGLSAERVPFLTSRICGICSISHSLASIRSLE
RAMQITPPETARIIRLLAMHGETLQSHALHLFFLAAPDFMGTPSVVPLMQSHPEVVEAGLLLKELGNELSIATTGRATHP
VSLVLGGVSKAPAKQRLAEIKQMIADRKPMLDRATDFFMTLRVPEFVRETEFISLHDGKSYPYIGGNLVSTDGVKRDEND
YLAMTNEYTVDFSTSKFTRLSRESSAAGALARFNNNYAQLHPRAKEAAAAFGLEPVCHNPFMNNIAQLVECHHLVADAEE
LIDRLLDDDLRNIKADYKPRAGAATGAVEAPRGVLYHYMETDESGKVVKGDCIIPTTQNNANIHYDLHALAEQSLAQGMG
EKEVEKLCEMLVRSYDPCISCSVH

Sequences:

>Translated_424_residues
MKVDFNIDIHHLPRVEGHGDIRISVRDGKLVDAKWAVVETPRFFEVMVKGLSAERVPFLTSRICGICSISHSLASIRSLE
RAMQITPPETARIIRLLAMHGETLQSHALHLFFLAAPDFMGTPSVVPLMQSHPEVVEAGLLLKELGNELSIATTGRATHP
VSLVLGGVSKAPAKQRLAEIKQMIADRKPMLDRATDFFMTLRVPEFVRETEFISLHDGKSYPYIGGNLVSTDGVKRDEND
YLAMTNEYTVDFSTSKFTRLSRESSAAGALARFNNNYAQLHPRAKEAAAAFGLEPVCHNPFMNNIAQLVECHHLVADAEE
LIDRLLDDDLRNIKADYKPRAGAATGAVEAPRGVLYHYMETDESGKVVKGDCIIPTTQNNANIHYDLHALAEQSLAQGMG
EKEVEKLCEMLVRSYDPCISCSVH
>Mature_424_residues
MKVDFNIDIHHLPRVEGHGDIRISVRDGKLVDAKWAVVETPRFFEVMVKGLSAERVPFLTSRICGICSISHSLASIRSLE
RAMQITPPETARIIRLLAMHGETLQSHALHLFFLAAPDFMGTPSVVPLMQSHPEVVEAGLLLKELGNELSIATTGRATHP
VSLVLGGVSKAPAKQRLAEIKQMIADRKPMLDRATDFFMTLRVPEFVRETEFISLHDGKSYPYIGGNLVSTDGVKRDEND
YLAMTNEYTVDFSTSKFTRLSRESSAAGALARFNNNYAQLHPRAKEAAAAFGLEPVCHNPFMNNIAQLVECHHLVADAEE
LIDRLLDDDLRNIKADYKPRAGAATGAVEAPRGVLYHYMETDESGKVVKGDCIIPTTQNNANIHYDLHALAEQSLAQGMG
EKEVEKLCEMLVRSYDPCISCSVH

Specific function: Part of a complex that provides reducing equivalents for heterodisulfide reductase [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788832, Length=409, Percent_Identity=24.2053789731051, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789076, Length=411, Percent_Identity=24.0875912408759, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789368, Length=127, Percent_Identity=31.496062992126, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46929; Mature: 46929

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDFNIDIHHLPRVEGHGDIRISVRDGKLVDAKWAVVETPRFFEVMVKGLSAERVPFLT
CEEEEEEEHHCCCCCCCCCCEEEEEECCEEEECEEEEECCCHHHHHHHHCCCCCCCHHHH
SRICGICSISHSLASIRSLERAMQITPPETARIIRLLAMHGETLQSHALHLFFLAAPDFM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCEEEEEEECCCCC
GTPSVVPLMQSHPEVVEAGLLLKELGNELSIATTGRATHPVSLVLGGVSKAPAKQRLAEI
CCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEECCCCCCCHHHHHHHH
KQMIADRKPMLDRATDFFMTLRVPEFVRETEFISLHDGKSYPYIGGNLVSTDGVKRDEND
HHHHHCCCCHHHHHHHHHEEECCHHHHHCCCEEEEECCCCCCEECCEEEECCCCCCCCCC
YLAMTNEYTVDFSTSKFTRLSRESSAAGALARFNNNYAQLHPRAKEAAAAFGLEPVCHNP
EEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHCCCHHHCCH
FMNNIAQLVECHHLVADAEELIDRLLDDDLRNIKADYKPRAGAATGAVEAPRGVLYHYME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEE
TDESGKVVKGDCIIPTTQNNANIHYDLHALAEQSLAQGMGEKEVEKLCEMLVRSYDPCIS
CCCCCCEEECCEEEECCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEE
CSVH
ECCC
>Mature Secondary Structure
MKVDFNIDIHHLPRVEGHGDIRISVRDGKLVDAKWAVVETPRFFEVMVKGLSAERVPFLT
CEEEEEEEHHCCCCCCCCCCEEEEEECCEEEECEEEEECCCHHHHHHHHCCCCCCCHHHH
SRICGICSISHSLASIRSLERAMQITPPETARIIRLLAMHGETLQSHALHLFFLAAPDFM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCEEEEEEECCCCC
GTPSVVPLMQSHPEVVEAGLLLKELGNELSIATTGRATHPVSLVLGGVSKAPAKQRLAEI
CCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHEECCCCCCCHHHHHHHH
KQMIADRKPMLDRATDFFMTLRVPEFVRETEFISLHDGKSYPYIGGNLVSTDGVKRDEND
HHHHHCCCCHHHHHHHHHEEECCHHHHHCCCEEEEECCCCCCEECCEEEECCCCCCCCCC
YLAMTNEYTVDFSTSKFTRLSRESSAAGALARFNNNYAQLHPRAKEAAAAFGLEPVCHNP
EEEEECEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHEECCCHHHHHHHHCCCHHHCCH
FMNNIAQLVECHHLVADAEELIDRLLDDDLRNIKADYKPRAGAATGAVEAPRGVLYHYME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEE
TDESGKVVKGDCIIPTTQNNANIHYDLHALAEQSLAQGMGEKEVEKLCEMLVRSYDPCIS
CCCCCCEEECCEEEECCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEE
CSVH
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA