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The map label for this gene is menD [H]

Identifier: 21674653

GI number: 21674653

Start: 1741176

End: 1742981

Strand: Direct

Name: menD [H]

Synonym: CT1839

Alternate gene names: 21674653

Gene position: 1741176-1742981 (Clockwise)

Preceding gene: 21674652

Following gene: 21674657

Centisome position: 80.8

GC content: 62.68

Gene sequence:

>1806_bases
GTGGGAAGAGGTCGATCAGAAAATCGGGGACATTCTTGCCATAACGCAGCAGACCTCATGAACAGCAAGCAGATCACCAC
GCTCTGGTGCGCGGTCATCGTCGAGGAACTGATCCGGCAGGAGGCCGGATTTTTCTGTATCTCCCCCGGCTCACGCTCGA
CGCCGCTGACCCTTGCCGTAGCCTCGAATCCGAAAGCGCGATTCAGGATGTTCCCCGACGAGCGCTCGGCGGGATTCTAC
GCGCTTGGGTACGCAAGAGCGACCGGAATGCCCGCCGTGCTCGTCTGCACCTCCGGCACCGCTGTGGCCAACTACTTCCC
CGCCGTCGTCGAAGCATCGGCGGACGCGCAGCCAATGCTCGTGCTCTCTGCTGACCGCCCCTTCGAGTTGCTCGAATGCG
GCGCGAACCAGGCCATCCGCCAGCAGAATATTTTCGGAAGCTACACACGCTGGAGCTTCGAGCTGCCCGAACCCGGCATT
GCGACGCCGCTCGCCTCGCTGCTTTCGACGGTGGATCACGCCGTCAGAAAAAGCCTCAGCCTCCCCGCAGGCCCGGTGCA
TCTGAACCTGCCGTTCCGCGAACCGCTCGAACCCGAAGCGCCCGATCCCGGTCATCCGTGGGCCGCGCCGCTCGAAACGT
GGCAGGCCTCCGGCGAACCGTGGAGCCGCTTCGCGCGTCCGCTGCACGAACCAAGCGCAGAGAGTATCGTGACGCTGCGG
GAGTTACTTGCACAGGCTGAACGGCCGCTGTTCGTGGCCGGAAGCATGTCGAACGCGGCGGATGGCGAGGCAGTAGCGGC
GCTAGCCGAATCGCTCGGTGTGCCGCTCTTCGCTGATCTCACCTCCGGCATCCGGCTTTCATCGGACTGCACGCCCTGGC
AGCTCGCCTTCCAGAATGAAGCCTTCGTCGAGCGCTTTCAGCCCGATGTAGTAATCCATTTCGGTGGCCATGTCATCGGC
AAGCAGCCCGCCATGGCATTGCGGAAACAGCCGCCCCTGCATTACGTGGTTGTCCGTGAGCATCCCGGACGCTTCGACCC
CGACCACAACGTCACGCTGACTCTCGAAGCTTCGCCCGCAGCCGTCGCTTCGGCGCTCGAAGGGTGCCGAGAGCCGGTGC
CGGGCATCAGATGCCGCGACGCATTTTCAGCAGCCTCCGGTATAATCGATAAAATGGCCTGCGTGCCGGAGCTTGCAGTC
AGCGAAATCTCCGCGCCGCGCATCGTTTCATCGCTCGCCGGAGACGGGCACGCGCTCTTCGTCGCAAACAGTATGCCCGC
GCGAGACATGGATCTGTACGCCGCGCCAGTAGCGCAGAAACCATTGCAGGTGGCGCTCAACCGGGGGGTCAGCGGCATCG
ACGGCATCATCTCGACCGCCGCCGGATTCTCGGCGGGCCTCGGCAAACCGACGACGCTGCTCATCGGCGACATCTCCTTC
CTGCACGACCTGAACGCCCTCTGCCTGCTCAACCACCCGTGGAACCCGCTCATCGTCATCGTGCTAAACAACCACGGCGG
CAGCATTTTCTCCTTCCTGCCCATCGCTTCGCAGACCGACCGGCTCGACGAGTGCTTTGCCACACCGCAGAACTTCAGCA
TCGAATCCGCCGCCCGCACCTTCGGCATCGACTACGCCTGCCCGGAGACAAACGGCGATTTCACACAGCTCTACGCAGAA
GCGCTCACAACAAAAAAAAGCCTCATCATCGAAATCAGAAGCGACCGGGAGAAAAACCTCCTTCTGCACCGCTCGCTCAA
GGCGCGACTCGACCCGGTTTTTGAAAAAGCTGACTGCTCCCGGTAA

Upstream 100 bases:

>100_bases
CGGAGTTTGCGGTCGGCATCCGCTCGGCACTCGTCAATGATGACCGCGTCTATCTCTACTCCGGTGCAGGACTGGTTCGT
GGATCGAATCCGGAGTCAGA

Downstream 100 bases:

>100_bases
AACGGTCATTCAGAGCATATCAGCGAAAAACACTTCAGCGTAGCGAAACCAGGCGACGGATCGCCCTCAAAGCCCATGCG
GGCGAGGTAGGACGGGAGAT

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MGRGRSENRGHSCHNAADLMNSKQITTLWCAVIVEELIRQEAGFFCISPGSRSTPLTLAVASNPKARFRMFPDERSAGFY
ALGYARATGMPAVLVCTSGTAVANYFPAVVEASADAQPMLVLSADRPFELLECGANQAIRQQNIFGSYTRWSFELPEPGI
ATPLASLLSTVDHAVRKSLSLPAGPVHLNLPFREPLEPEAPDPGHPWAAPLETWQASGEPWSRFARPLHEPSAESIVTLR
ELLAQAERPLFVAGSMSNAADGEAVAALAESLGVPLFADLTSGIRLSSDCTPWQLAFQNEAFVERFQPDVVIHFGGHVIG
KQPAMALRKQPPLHYVVVREHPGRFDPDHNVTLTLEASPAAVASALEGCREPVPGIRCRDAFSAASGIIDKMACVPELAV
SEISAPRIVSSLAGDGHALFVANSMPARDMDLYAAPVAQKPLQVALNRGVSGIDGIISTAAGFSAGLGKPTTLLIGDISF
LHDLNALCLLNHPWNPLIVIVLNNHGGSIFSFLPIASQTDRLDECFATPQNFSIESAARTFGIDYACPETNGDFTQLYAE
ALTTKKSLIIEIRSDREKNLLLHRSLKARLDPVFEKADCSR

Sequences:

>Translated_601_residues
MGRGRSENRGHSCHNAADLMNSKQITTLWCAVIVEELIRQEAGFFCISPGSRSTPLTLAVASNPKARFRMFPDERSAGFY
ALGYARATGMPAVLVCTSGTAVANYFPAVVEASADAQPMLVLSADRPFELLECGANQAIRQQNIFGSYTRWSFELPEPGI
ATPLASLLSTVDHAVRKSLSLPAGPVHLNLPFREPLEPEAPDPGHPWAAPLETWQASGEPWSRFARPLHEPSAESIVTLR
ELLAQAERPLFVAGSMSNAADGEAVAALAESLGVPLFADLTSGIRLSSDCTPWQLAFQNEAFVERFQPDVVIHFGGHVIG
KQPAMALRKQPPLHYVVVREHPGRFDPDHNVTLTLEASPAAVASALEGCREPVPGIRCRDAFSAASGIIDKMACVPELAV
SEISAPRIVSSLAGDGHALFVANSMPARDMDLYAAPVAQKPLQVALNRGVSGIDGIISTAAGFSAGLGKPTTLLIGDISF
LHDLNALCLLNHPWNPLIVIVLNNHGGSIFSFLPIASQTDRLDECFATPQNFSIESAARTFGIDYACPETNGDFTQLYAE
ALTTKKSLIIEIRSDREKNLLLHRSLKARLDPVFEKADCSR
>Mature_600_residues
GRGRSENRGHSCHNAADLMNSKQITTLWCAVIVEELIRQEAGFFCISPGSRSTPLTLAVASNPKARFRMFPDERSAGFYA
LGYARATGMPAVLVCTSGTAVANYFPAVVEASADAQPMLVLSADRPFELLECGANQAIRQQNIFGSYTRWSFELPEPGIA
TPLASLLSTVDHAVRKSLSLPAGPVHLNLPFREPLEPEAPDPGHPWAAPLETWQASGEPWSRFARPLHEPSAESIVTLRE
LLAQAERPLFVAGSMSNAADGEAVAALAESLGVPLFADLTSGIRLSSDCTPWQLAFQNEAFVERFQPDVVIHFGGHVIGK
QPAMALRKQPPLHYVVVREHPGRFDPDHNVTLTLEASPAAVASALEGCREPVPGIRCRDAFSAASGIIDKMACVPELAVS
EISAPRIVSSLAGDGHALFVANSMPARDMDLYAAPVAQKPLQVALNRGVSGIDGIISTAAGFSAGLGKPTTLLIGDISFL
HDLNALCLLNHPWNPLIVIVLNNHGGSIFSFLPIASQTDRLDECFATPQNFSIESAARTFGIDYACPETNGDFTQLYAEA
LTTKKSLIIEIRSDREKNLLLHRSLKARLDPVFEKADCSR

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=561, Percent_Identity=38.3244206773619, Blast_Score=312, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6323755, Length=239, Percent_Identity=26.7782426778243, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 64740; Mature: 64609

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRGRSENRGHSCHNAADLMNSKQITTLWCAVIVEELIRQEAGFFCISPGSRSTPLTLAV
CCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEE
ASNPKARFRMFPDERSAGFYALGYARATGMPAVLVCTSGTAVANYFPAVVEASADAQPML
ECCCCCEEEECCCCCCCCEEEEEEHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEE
VLSADRPFELLECGANQAIRQQNIFGSYTRWSFELPEPGIATPLASLLSTVDHAVRKSLS
EEECCCCHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
LPAGPVHLNLPFREPLEPEAPDPGHPWAAPLETWQASGEPWSRFARPLHEPSAESIVTLR
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHH
ELLAQAERPLFVAGSMSNAADGEAVAALAESLGVPLFADLTSGIRLSSDCTPWQLAFQNE
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEHHCCCCEECCCCCCEEEEECCH
AFVERFQPDVVIHFGGHVIGKQPAMALRKQPPLHYVVVREHPGRFDPDHNVTLTLEASPA
HHHHHCCCCEEEEECCEEECCCCHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCHH
AVASALEGCREPVPGIRCRDAFSAASGIIDKMACVPELAVSEISAPRIVSSLAGDGHALF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEE
VANSMPARDMDLYAAPVAQKPLQVALNRGVSGIDGIISTAAGFSAGLGKPTTLLIGDISF
EECCCCCCCCCCEECCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECHHH
LHDLNALCLLNHPWNPLIVIVLNNHGGSIFSFLPIASQTDRLDECFATPQNFSIESAART
HHCCCCEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHH
FGIDYACPETNGDFTQLYAEALTTKKSLIIEIRSDREKNLLLHRSLKARLDPVFEKADCS
HCCEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
R
C
>Mature Secondary Structure 
GRGRSENRGHSCHNAADLMNSKQITTLWCAVIVEELIRQEAGFFCISPGSRSTPLTLAV
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEE
ASNPKARFRMFPDERSAGFYALGYARATGMPAVLVCTSGTAVANYFPAVVEASADAQPML
ECCCCCEEEECCCCCCCCEEEEEEHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCEEE
VLSADRPFELLECGANQAIRQQNIFGSYTRWSFELPEPGIATPLASLLSTVDHAVRKSLS
EEECCCCHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
LPAGPVHLNLPFREPLEPEAPDPGHPWAAPLETWQASGEPWSRFARPLHEPSAESIVTLR
CCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCCCHHHHHHHH
ELLAQAERPLFVAGSMSNAADGEAVAALAESLGVPLFADLTSGIRLSSDCTPWQLAFQNE
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEHHCCCCEECCCCCCEEEEECCH
AFVERFQPDVVIHFGGHVIGKQPAMALRKQPPLHYVVVREHPGRFDPDHNVTLTLEASPA
HHHHHCCCCEEEEECCEEECCCCHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCHH
AVASALEGCREPVPGIRCRDAFSAASGIIDKMACVPELAVSEISAPRIVSSLAGDGHALF
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEE
VANSMPARDMDLYAAPVAQKPLQVALNRGVSGIDGIISTAAGFSAGLGKPTTLLIGDISF
EECCCCCCCCCCEECCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECHHH
LHDLNALCLLNHPWNPLIVIVLNNHGGSIFSFLPIASQTDRLDECFATPQNFSIESAART
HHCCCCEEEECCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHH
FGIDYACPETNGDFTQLYAEALTTKKSLIIEIRSDREKNLLLHRSLKARLDPVFEKADCS
HCCEEECCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]