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The map label for this gene is menF [H]

Identifier: 21674652

GI number: 21674652

Start: 1739770

End: 1741236

Strand: Direct

Name: menF [H]

Synonym: CT1838

Alternate gene names: 21674652

Gene position: 1739770-1741236 (Clockwise)

Preceding gene: 21674651

Following gene: 21674653

Centisome position: 80.73

GC content: 59.1

Gene sequence:

>1467_bases
TTGAGCGGCACCTGCAAAACGATGTGCCTGACAAGGCCGGAAAATGAACCTCGTAATCCCATGCAGGCGGACGACAGTAT
CCCTATGATGCAAAAGGCGCTTCAACTCCTCGAAGCATCGGTAAAAGCCGCGATGCGGCAACGAGACGGCTCACCTGCCG
TTAGCGGGCAGCCGATGCTGGAGCGCTTCTCGGCGCCGCTCGGCGAGGTTGACGCCACGCGGTGGCTGTCGGCGCAGAGG
CTTTTCCCGAGGCTGTTCTGGATGAACCGCGAGAAAAGCGAGTGGATTGCCGGTATCGGAGAAGCTGACCGTATCGAGAT
CACCGAAAGCGGCCCCAACGACCGCAGCTTCCTCGTGCTCGAAGAGGCCATGACGCGGAAAAATCCGCACGCCAGATACA
TCGGCGGTTTCTGCTTCAACAATCTCCAGAAGCAGAACAAACTCTGGAGCGCTTTCAGCCCCGGCCTTTTCATATTGCCG
CTTGTCAGCATCGAATACCGCGACGGCCAGACACTGATGACCTGCTCGCTGTGGCTGGAGCCAGGCAACGACCGTCAGAA
AGGGCTCGAACAGCTCTTGGCAGCCTTGTCGGCAGTCTCCGCCGGAGACGCGCCAGAGACTGCCGGGATTCCGGAAATGA
CGCAGGTCTCCTATTGCCCCGACAAGGCGCAATGGATCGAGAACTGCGAGACGATTCTGAGAAATTTCGATGAGGGAAAA
CTCGACAAGGTGATCCTCGCCCGGCAGACTGAACTTTCTTTCGCAGGCAAGGTTCCTGCCATCCGTTTCCTGCTCGACTA
TCCATTTCCCGAGAATACCGCGTACCGTTTCTACTTCGAGCCAGTCGAGGGACACGCCTTCATCAGCTTCACGCCTGAAC
GGCTCTACCGCCGCGACGGCGACATGCTCGAAACCGAAGCGCTCGCAGGCACGGTCACCAAAGAGGCGCTCAAGGCGGAC
GACAGCATTGCTTCCGAGTTGCTGCTGAACTCCGAAAAGGACATCCGTGAGCACCGCTTCGTCAAGGACACGATTTATCG
CGAGTTGCAGCCGGTATGCAGCGACATCGACATGCAGGAGAAGGTCGGCGTGCTCCAGCTCAACCGCCTCGCCCATCTCC
TGGCCAAATGCAAGGCCAGGCTTCTTCCGGAGTTCAGCAACGACAGCACCGTGCTGCGTCAGCTTCATCCAACTCCGGCG
GTCGGCGGCGTGCCGAGGGAAAAGGCAATGTCGCTGATTCTGTCGATCGAGCCGTTCTGCCGTGGCTGGTACGCCGCGCC
AGTGGGCTGGCTCAACCGCGACGCAGCGGAGTTTGCGGTCGGCATCCGCTCGGCACTCGTCAATGATGACCGCGTCTATC
TCTACTCCGGTGCAGGACTGGTTCGTGGATCGAATCCGGAGTCAGAGTGGGAAGAGGTCGATCAGAAAATCGGGGACATT
CTTGCCATAACGCAGCAGACCTCATGA

Upstream 100 bases:

>100_bases
TACTCGATGGCGGTCTGCTACATTAAAATGGGAAAAATCGATGAAGCCCGAAAAATCCATAAATGGCTTCAGACCAAAAA
CAATTATCTTGCCGTCTCCC

Downstream 100 bases:

>100_bases
ACAGCAAGCAGATCACCACGCTCTGGTGCGCGGTCATCGTCGAGGAACTGATCCGGCAGGAGGCCGGATTTTTCTGTATC
TCCCCCGGCTCACGCTCGAC

Product: menaquinone-specific isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MSGTCKTMCLTRPENEPRNPMQADDSIPMMQKALQLLEASVKAAMRQRDGSPAVSGQPMLERFSAPLGEVDATRWLSAQR
LFPRLFWMNREKSEWIAGIGEADRIEITESGPNDRSFLVLEEAMTRKNPHARYIGGFCFNNLQKQNKLWSAFSPGLFILP
LVSIEYRDGQTLMTCSLWLEPGNDRQKGLEQLLAALSAVSAGDAPETAGIPEMTQVSYCPDKAQWIENCETILRNFDEGK
LDKVILARQTELSFAGKVPAIRFLLDYPFPENTAYRFYFEPVEGHAFISFTPERLYRRDGDMLETEALAGTVTKEALKAD
DSIASELLLNSEKDIREHRFVKDTIYRELQPVCSDIDMQEKVGVLQLNRLAHLLAKCKARLLPEFSNDSTVLRQLHPTPA
VGGVPREKAMSLILSIEPFCRGWYAAPVGWLNRDAAEFAVGIRSALVNDDRVYLYSGAGLVRGSNPESEWEEVDQKIGDI
LAITQQTS

Sequences:

>Translated_488_residues
MSGTCKTMCLTRPENEPRNPMQADDSIPMMQKALQLLEASVKAAMRQRDGSPAVSGQPMLERFSAPLGEVDATRWLSAQR
LFPRLFWMNREKSEWIAGIGEADRIEITESGPNDRSFLVLEEAMTRKNPHARYIGGFCFNNLQKQNKLWSAFSPGLFILP
LVSIEYRDGQTLMTCSLWLEPGNDRQKGLEQLLAALSAVSAGDAPETAGIPEMTQVSYCPDKAQWIENCETILRNFDEGK
LDKVILARQTELSFAGKVPAIRFLLDYPFPENTAYRFYFEPVEGHAFISFTPERLYRRDGDMLETEALAGTVTKEALKAD
DSIASELLLNSEKDIREHRFVKDTIYRELQPVCSDIDMQEKVGVLQLNRLAHLLAKCKARLLPEFSNDSTVLRQLHPTPA
VGGVPREKAMSLILSIEPFCRGWYAAPVGWLNRDAAEFAVGIRSALVNDDRVYLYSGAGLVRGSNPESEWEEVDQKIGDI
LAITQQTS
>Mature_487_residues
SGTCKTMCLTRPENEPRNPMQADDSIPMMQKALQLLEASVKAAMRQRDGSPAVSGQPMLERFSAPLGEVDATRWLSAQRL
FPRLFWMNREKSEWIAGIGEADRIEITESGPNDRSFLVLEEAMTRKNPHARYIGGFCFNNLQKQNKLWSAFSPGLFILPL
VSIEYRDGQTLMTCSLWLEPGNDRQKGLEQLLAALSAVSAGDAPETAGIPEMTQVSYCPDKAQWIENCETILRNFDEGKL
DKVILARQTELSFAGKVPAIRFLLDYPFPENTAYRFYFEPVEGHAFISFTPERLYRRDGDMLETEALAGTVTKEALKADD
SIASELLLNSEKDIREHRFVKDTIYRELQPVCSDIDMQEKVGVLQLNRLAHLLAKCKARLLPEFSNDSTVLRQLHPTPAV
GGVPREKAMSLILSIEPFCRGWYAAPVGWLNRDAAEFAVGIRSALVNDDRVYLYSGAGLVRGSNPESEWEEVDQKIGDIL
AITQQTS

Specific function: Menaquinone biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI87082077, Length=424, Percent_Identity=33.0188679245283, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1786809, Length=246, Percent_Identity=29.6747967479675, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788114, Length=266, Percent_Identity=25.187969924812, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=208, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 54790; Mature: 54659

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTCKTMCLTRPENEPRNPMQADDSIPMMQKALQLLEASVKAAMRQRDGSPAVSGQPML
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ERFSAPLGEVDATRWLSAQRLFPRLFWMNREKSEWIAGIGEADRIEITESGPNDRSFLVL
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEE
EEAMTRKNPHARYIGGFCFNNLQKQNKLWSAFSPGLFILPLVSIEYRDGQTLMTCSLWLE
EHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEEEEEEEC
PGNDRQKGLEQLLAALSAVSAGDAPETAGIPEMTQVSYCPDKAQWIENCETILRNFDEGK
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
LDKVILARQTELSFAGKVPAIRFLLDYPFPENTAYRFYFEPVEGHAFISFTPERLYRRDG
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCEEEEECHHHHHHCCC
DMLETEALAGTVTKEALKADDSIASELLLNSEKDIREHRFVKDTIYRELQPVCSDIDMQE
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KVGVLQLNRLAHLLAKCKARLLPEFSNDSTVLRQLHPTPAVGGVPREKAMSLILSIEPFC
HCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHH
RGWYAAPVGWLNRDAAEFAVGIRSALVNDDRVYLYSGAGLVRGSNPESEWEEVDQKIGDI
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHE
LAITQQTS
EEEECCCC
>Mature Secondary Structure 
SGTCKTMCLTRPENEPRNPMQADDSIPMMQKALQLLEASVKAAMRQRDGSPAVSGQPML
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ERFSAPLGEVDATRWLSAQRLFPRLFWMNREKSEWIAGIGEADRIEITESGPNDRSFLVL
HHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCCEEEEE
EEAMTRKNPHARYIGGFCFNNLQKQNKLWSAFSPGLFILPLVSIEYRDGQTLMTCSLWLE
EHHHHCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCEEEEEEEEEC
PGNDRQKGLEQLLAALSAVSAGDAPETAGIPEMTQVSYCPDKAQWIENCETILRNFDEGK
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
LDKVILARQTELSFAGKVPAIRFLLDYPFPENTAYRFYFEPVEGHAFISFTPERLYRRDG
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCEEEEEEEECCCCEEEEECHHHHHHCCC
DMLETEALAGTVTKEALKADDSIASELLLNSEKDIREHRFVKDTIYRELQPVCSDIDMQE
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KVGVLQLNRLAHLLAKCKARLLPEFSNDSTVLRQLHPTPAVGGVPREKAMSLILSIEPFC
HCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHH
RGWYAAPVGWLNRDAAEFAVGIRSALVNDDRVYLYSGAGLVRGSNPESEWEEVDQKIGDI
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHE
LAITQQTS
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]