Click here to switch to the map view.

The map label for this gene is gcvT

Identifier: 21674602

GI number: 21674602

Start: 1694721

End: 1695818

Strand: Reverse

Name: gcvT

Synonym: CT1788

Alternate gene names: 21674602

Gene position: 1695818-1694721 (Counterclockwise)

Preceding gene: 21674603

Following gene: 21674601

Centisome position: 78.69

GC content: 59.74

Gene sequence:

>1098_bases
ATGAAAAAGACTGCGCTCTCAGCCTGGCATGAAGCTGCCGGTGCGAAAATGATCGATTTCGGCGGATTTCTGATGCCGGT
GCAGTACACCGGGATTATCGCCGAGCACAAGGCCGTCCGCGAAGCGGCGGGGCTTTTCGATGTGTCTCACATGGGTAATT
TCTACGTCAGGGGAGCGCGGGCGCTGGAGTTCCTTCAGTACATGACCACCAACGATCTCGCAAAAATCGTGGACGGGCAA
GCGCAGTACACGCTGATGCTCTATCCCGATGGCGGCATCGTCGATGACCTTATCATCTACCGGGTGAGCGCCGACACCTT
TTTCCTCATCGTCAACGCGAGCAACTGCGAAAAGGATTTCGACTGGCTTTCGAGCCATATCGGCCAGTTCGAGGGGGTGG
CGCTCGAAAACCACACCAGCGAGCTTTCGCTCATCGCCCTGCAGGGGCCGAAGTCGTTCGATATTCTGGCGCGGGTTTTC
CCTGGCGCGGGCATCGACAAGCTCGGTTCGTTCCACTTCATAAAGCTGCCGTTCGAGGGAGCGGAGATCATGGTGGCCCG
CACCGGCTACACCGGCGAGGCGGGCGTCGAAATCTGCCTTCCGAACGAAAGGGCGGTTGCGCTCTGGTCGGCGCTGATGG
AGGCAGGAAAGAGTGACGGCATCCAGCCGATCGGTCTCGGCGCGCGCGATACGTTGCGGCTGGAGATGGGCTATTCGCTC
TACGGCCACGAGATCGAGAGGGATGTCAATCCGCTCGAAGCACGGCTCAAATGGGTGGTGAAGCTGAACAAACCAAACTT
CATCGGCAAGCAGGCGTGCGAGCAGGTTGAGATCAATCCACGCAAGAGCGTGGTTGGCTTCAGCCTCGAAGGCCGCGCTA
TTCCGCGCCAGCACTTCAAGGTCTATAACTCCGACAAGCAGGAGATTGGTGAGGTGTGCAGCGGCACGGTGTCGCCGACC
CTTCAGGAGCCGATTGGCACGGCGAGCCTTCTGCTCGACTACGCGCAGCCCGGTACGCCGATCTTCGTTGAAATTCGCGG
AACCATGCAACCCGGTGCGGTCAGGCGCCTGCCGTTCGTGCACGCTGACCGTCCATGA

Upstream 100 bases:

>100_bases
AAAACCAGGTGTCGCAAAGAGTTGAAAAAGGCTTTGAACTTTTCCGCAAATTGTTTTTTTACAAGGCATTCCAACATAGC
TATCCAAATAAAGGAGATCA

Downstream 100 bases:

>100_bases
ACTGATCACAAGCCGGTCTCCCCCTTGAAAAACAAGATCAACATAAAGGCTTTCTGCACGGATAGTCTCGTCACTGAAGT
TGCTGGTATCGCCTTGATGC

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MKKTALSAWHEAAGAKMIDFGGFLMPVQYTGIIAEHKAVREAAGLFDVSHMGNFYVRGARALEFLQYMTTNDLAKIVDGQ
AQYTLMLYPDGGIVDDLIIYRVSADTFFLIVNASNCEKDFDWLSSHIGQFEGVALENHTSELSLIALQGPKSFDILARVF
PGAGIDKLGSFHFIKLPFEGAEIMVARTGYTGEAGVEICLPNERAVALWSALMEAGKSDGIQPIGLGARDTLRLEMGYSL
YGHEIERDVNPLEARLKWVVKLNKPNFIGKQACEQVEINPRKSVVGFSLEGRAIPRQHFKVYNSDKQEIGEVCSGTVSPT
LQEPIGTASLLLDYAQPGTPIFVEIRGTMQPGAVRRLPFVHADRP

Sequences:

>Translated_365_residues
MKKTALSAWHEAAGAKMIDFGGFLMPVQYTGIIAEHKAVREAAGLFDVSHMGNFYVRGARALEFLQYMTTNDLAKIVDGQ
AQYTLMLYPDGGIVDDLIIYRVSADTFFLIVNASNCEKDFDWLSSHIGQFEGVALENHTSELSLIALQGPKSFDILARVF
PGAGIDKLGSFHFIKLPFEGAEIMVARTGYTGEAGVEICLPNERAVALWSALMEAGKSDGIQPIGLGARDTLRLEMGYSL
YGHEIERDVNPLEARLKWVVKLNKPNFIGKQACEQVEINPRKSVVGFSLEGRAIPRQHFKVYNSDKQEIGEVCSGTVSPT
LQEPIGTASLLLDYAQPGTPIFVEIRGTMQPGAVRRLPFVHADRP
>Mature_365_residues
MKKTALSAWHEAAGAKMIDFGGFLMPVQYTGIIAEHKAVREAAGLFDVSHMGNFYVRGARALEFLQYMTTNDLAKIVDGQ
AQYTLMLYPDGGIVDDLIIYRVSADTFFLIVNASNCEKDFDWLSSHIGQFEGVALENHTSELSLIALQGPKSFDILARVF
PGAGIDKLGSFHFIKLPFEGAEIMVARTGYTGEAGVEICLPNERAVALWSALMEAGKSDGIQPIGLGARDTLRLEMGYSL
YGHEIERDVNPLEARLKWVVKLNKPNFIGKQACEQVEINPRKSVVGFSLEGRAIPRQHFKVYNSDKQEIGEVCSGTVSPT
LQEPIGTASLLLDYAQPGTPIFVEIRGTMQPGAVRRLPFVHADRP

Specific function: The glycine cleavage system catalyzes the degradation of glycine

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family

Homologues:

Organism=Homo sapiens, GI44662838, Length=378, Percent_Identity=32.5396825396825, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI257796258, Length=363, Percent_Identity=32.2314049586777, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI257796256, Length=325, Percent_Identity=31.0769230769231, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI257796254, Length=374, Percent_Identity=29.6791443850267, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI24797151, Length=332, Percent_Identity=28.9156626506024, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI197927446, Length=307, Percent_Identity=27.6872964169381, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI21361378, Length=307, Percent_Identity=27.6872964169381, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI194306651, Length=262, Percent_Identity=30.1526717557252, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1789272, Length=368, Percent_Identity=39.1304347826087, Blast_Score=241, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17560118, Length=375, Percent_Identity=32.8, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71994045, Length=315, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71994052, Length=315, Percent_Identity=26.6666666666667, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32563613, Length=274, Percent_Identity=27.007299270073, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320222, Length=382, Percent_Identity=35.0785340314136, Blast_Score=204, Evalue=2e-53,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=32.3607427055703, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28571104, Length=257, Percent_Identity=29.9610894941634, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130091, Length=262, Percent_Identity=25.1908396946565, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): GCST_CHLTE (Q8KBJ9)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662667.1
- ProteinModelPortal:   Q8KBJ9
- SMR:   Q8KBJ9
- GeneID:   1008275
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1788
- NMPDR:   fig|194439.1.peg.1761
- TIGR:   CT1788
- HOGENOM:   HBG299834
- OMA:   KALYGGM
- ProtClustDB:   PRK00389
- BioCyc:   CTEP194439:CT_1788-MONOMER
- BRENDA:   2.1.2.10
- GO:   GO:0005737
- HAMAP:   MF_00259
- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903
- PIRSF:   PIRSF006487
- TIGRFAMs:   TIGR00528

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C

EC number: =2.1.2.10

Molecular weight: Translated: 40140; Mature: 40140

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTALSAWHEAAGAKMIDFGGFLMPVQYTGIIAEHKAVREAAGLFDVSHMGNFYVRGAR
CCCHHHHHHHHHCCCEEEECCCEEEEEEEECCHHHHHHHHHHHCCEEHHHCCCEEEEHHH
ALEFLQYMTTNDLAKIVDGQAQYTLMLYPDGGIVDDLIIYRVSADTFFLIVNASNCEKDF
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCEEEEEEECCCCCHHH
DWLSSHIGQFEGVALENHTSELSLIALQGPKSFDILARVFPGAGIDKLGSFHFIKLPFEG
HHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCHHHCCCEEEEEECCCC
AEIMVARTGYTGEAGVEICLPNERAVALWSALMEAGKSDGIQPIGLGARDTLRLEMGYSL
CEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCC
YGHEIERDVNPLEARLKWVVKLNKPNFIGKQACEQVEINPRKSVVGFSLEGRAIPRQHFK
CCHHHHCCCCHHHHEEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHE
VYNSDKQEIGEVCSGTVSPTLQEPIGTASLLLDYAQPGTPIFVEIRGTMQPGAVRRLPFV
EECCCHHHHHHHHCCCCCCHHHCCCCHHHHHEECCCCCCEEEEEEECCCCCCCEEECCEE
HADRP
ECCCC
>Mature Secondary Structure
MKKTALSAWHEAAGAKMIDFGGFLMPVQYTGIIAEHKAVREAAGLFDVSHMGNFYVRGAR
CCCHHHHHHHHHCCCEEEECCCEEEEEEEECCHHHHHHHHHHHCCEEHHHCCCEEEEHHH
ALEFLQYMTTNDLAKIVDGQAQYTLMLYPDGGIVDDLIIYRVSADTFFLIVNASNCEKDF
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEEECCEEEEEEECCCCCHHH
DWLSSHIGQFEGVALENHTSELSLIALQGPKSFDILARVFPGAGIDKLGSFHFIKLPFEG
HHHHHHCCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHCCCCCHHHCCCEEEEEECCCC
AEIMVARTGYTGEAGVEICLPNERAVALWSALMEAGKSDGIQPIGLGARDTLRLEMGYSL
CEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCC
YGHEIERDVNPLEARLKWVVKLNKPNFIGKQACEQVEINPRKSVVGFSLEGRAIPRQHFK
CCHHHHCCCCHHHHEEEEEEEECCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHE
VYNSDKQEIGEVCSGTVSPTLQEPIGTASLLLDYAQPGTPIFVEIRGTMQPGAVRRLPFV
EECCCHHHHHHHHCCCCCCHHHCCCCHHHHHEECCCCCCEEEEEEECCCCCCCEEECCEE
HADRP
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901