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The map label for this gene is mesJ

Identifier: 21674432

GI number: 21674432

Start: 1520000

End: 1520995

Strand: Reverse

Name: mesJ

Synonym: CT1614

Alternate gene names: 21674432

Gene position: 1520995-1520000 (Counterclockwise)

Preceding gene: 21674435

Following gene: 21674426

Centisome position: 70.58

GC content: 57.03

Gene sequence:

>996_bases
GTGAACAAGACCGAGAAAAAATTTCTTGAAAACATGCATCGGCAGAAGCTTGTCGCGAACGGCGACGCGGTGCTGCTGGC
CGTTTCCGGCGGCCCGGACTCGATGGCGCTGCTGCATCTGTTTGCGTCGGTTGCGTCGGTGCTGCATTGCCGCCTCGGCG
TGGCGCACTGCAATTTCATGCTGCGTGGGGATGCGAGTGATGCCGACGAATCGTTCGTCCGCGATGCGTGCGCCGAGCTT
GGCATCGACTTTCATGTTCGGCGATTCGATACGGCGTCGGTCTCATCAGCCTGGAAAAAATCGATTGAAGAGACGGCCCG
CTTGCTGCGTTACGACTTTTTCGGCGAGCTTTGCCGCGAGGCCAGTTATACCCGGATCGCCACCGGGCACCACAGCGATG
ACAATGCCGAGACCGTGCTTTTCAATCTCTTTCGTGGCGCAGGGATTTCAGGTCTGCGGGGAATTCGGGTACGGCACGGT
GCAATCATCAGACCTCTTTTGCCCTTTACCCGACGGGAGATCGTAGCATATCTCGAAGAAAAACGGGTCGTCTGGAGAGA
TGATCACACCAACGAAGGAATCGAGTACGACCGTAATTTTATCCGCAATCGTGTGATTCCGGTGATCGAGGAGCGTTTTG
CGCACAAGCTCATGCCGTCGCTGCAACGCATTTCAGAACATGCGGGCGAACTCGAAGAGTTCATCGATCTCCACATCTCG
CGCCTGCTCGAAGCGCATCCAGGCCTCGACCTTGCAGGCGGCAAGCTGCACGTCGGTACGATGCGGCAACTATCGATGTT
CGAGCGAAAAGAGATTCTGAAGCGGGCCTTGAAGTTACAGGGGCTTTCGGTGGGCAGCAATGTCTTGAACAGAATTGCGG
GACTGCTCGACAATCAGGCGGGCCGGAGCGTTCCGGCTGGCGCGGGCGTCGAGGTGGTTCTGCATGACGGATTCCTGCGA
TTCCGGCAAACCGGTAATCCATCAGATCATCGGTAA

Upstream 100 bases:

>100_bases
TTGCAAAAACGGCCGAGCGTATATATTATGATGCTCTGCGATGGCCGGGAGTGCCGCATCGAGCGCCAATTCCACATCGA
GCAAATCGACAAACGATCCA

Downstream 100 bases:

>100_bases
CGTGCTCACTCCTTTTTGCCGCTTTTCTTGAAAATGGTCTCCGGCAGGTCAGCCGTGACGATGACCGATTCGAGGTTCTG
GCTCACCTTGAGGTGTTTGA

Product: MesJ protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNKTEKKFLENMHRQKLVANGDAVLLAVSGGPDSMALLHLFASVASVLHCRLGVAHCNFMLRGDASDADESFVRDACAEL
GIDFHVRRFDTASVSSAWKKSIEETARLLRYDFFGELCREASYTRIATGHHSDDNAETVLFNLFRGAGISGLRGIRVRHG
AIIRPLLPFTRREIVAYLEEKRVVWRDDHTNEGIEYDRNFIRNRVIPVIEERFAHKLMPSLQRISEHAGELEEFIDLHIS
RLLEAHPGLDLAGGKLHVGTMRQLSMFERKEILKRALKLQGLSVGSNVLNRIAGLLDNQAGRSVPAGAGVEVVLHDGFLR
FRQTGNPSDHR

Sequences:

>Translated_331_residues
MNKTEKKFLENMHRQKLVANGDAVLLAVSGGPDSMALLHLFASVASVLHCRLGVAHCNFMLRGDASDADESFVRDACAEL
GIDFHVRRFDTASVSSAWKKSIEETARLLRYDFFGELCREASYTRIATGHHSDDNAETVLFNLFRGAGISGLRGIRVRHG
AIIRPLLPFTRREIVAYLEEKRVVWRDDHTNEGIEYDRNFIRNRVIPVIEERFAHKLMPSLQRISEHAGELEEFIDLHIS
RLLEAHPGLDLAGGKLHVGTMRQLSMFERKEILKRALKLQGLSVGSNVLNRIAGLLDNQAGRSVPAGAGVEVVLHDGFLR
FRQTGNPSDHR
>Mature_331_residues
MNKTEKKFLENMHRQKLVANGDAVLLAVSGGPDSMALLHLFASVASVLHCRLGVAHCNFMLRGDASDADESFVRDACAEL
GIDFHVRRFDTASVSSAWKKSIEETARLLRYDFFGELCREASYTRIATGHHSDDNAETVLFNLFRGAGISGLRGIRVRHG
AIIRPLLPFTRREIVAYLEEKRVVWRDDHTNEGIEYDRNFIRNRVIPVIEERFAHKLMPSLQRISEHAGELEEFIDLHIS
RLLEAHPGLDLAGGKLHVGTMRQLSMFERKEILKRALKLQGLSVGSNVLNRIAGLLDNQAGRSVPAGAGVEVVLHDGFLR
FRQTGNPSDHR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=213, Percent_Identity=32.8638497652582, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_CHLTE (Q8KC15)

Other databases:

- EMBL:   AY005137
- EMBL:   AE006470
- RefSeq:   NP_662497.1
- ProteinModelPortal:   Q8KC15
- SMR:   Q8KC15
- GeneID:   1006109
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1614
- NMPDR:   fig|194439.1.peg.1591
- TIGR:   CT1614
- HOGENOM:   HBG405712
- OMA:   FNLFRGT
- ProtClustDB:   CLSK637855
- BioCyc:   CTEP194439:CT_1614-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 37136; Mature: 37136

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTEKKFLENMHRQKLVANGDAVLLAVSGGPDSMALLHLFASVASVLHCRLGVAHCNFM
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEE
LRGDASDADESFVRDACAELGIDFHVRRFDTASVSSAWKKSIEETARLLRYDFFGELCRE
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYTRIATGHHSDDNAETVLFNLFRGAGISGLRGIRVRHGAIIRPLLPFTRREIVAYLEE
CCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
KRVVWRDDHTNEGIEYDRNFIRNRVIPVIEERFAHKLMPSLQRISEHAGELEEFIDLHIS
CCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLEAHPGLDLAGGKLHVGTMRQLSMFERKEILKRALKLQGLSVGSNVLNRIAGLLDNQA
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GRSVPAGAGVEVVLHDGFLRFRQTGNPSDHR
CCCCCCCCCEEEEEECCHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKTEKKFLENMHRQKLVANGDAVLLAVSGGPDSMALLHLFASVASVLHCRLGVAHCNFM
CCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEE
LRGDASDADESFVRDACAELGIDFHVRRFDTASVSSAWKKSIEETARLLRYDFFGELCRE
EECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASYTRIATGHHSDDNAETVLFNLFRGAGISGLRGIRVRHGAIIRPLLPFTRREIVAYLEE
CCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
KRVVWRDDHTNEGIEYDRNFIRNRVIPVIEERFAHKLMPSLQRISEHAGELEEFIDLHIS
CCCEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLLEAHPGLDLAGGKLHVGTMRQLSMFERKEILKRALKLQGLSVGSNVLNRIAGLLDNQA
HHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GRSVPAGAGVEVVLHDGFLRFRQTGNPSDHR
CCCCCCCCCEEEEEECCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10976061; 12093901