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The map label for this gene is hemK [H]

Identifier: 21674307

GI number: 21674307

Start: 1392405

End: 1393289

Strand: Reverse

Name: hemK [H]

Synonym: CT1487

Alternate gene names: 21674307

Gene position: 1393289-1392405 (Counterclockwise)

Preceding gene: 21674310

Following gene: 21674306

Centisome position: 64.66

GC content: 61.36

Gene sequence:

>885_bases
ATGCCCGAAGAGAAAGTGTGGAGCGTTGTCGAGCTGCTCAAGACGACCATCGCGTTTTTTGCCGAGAAGAAGATCGACGA
ACCTCGTCTTTCAGCGGAGTTGCTTTTGGGGCATGTTTTGGGATTGCAGCGGCTTCAGCTTTATCTCGATCACGAGCGGC
CGTTGACGCTGAAAGAGCTTGAGGCGTTCCGGGCGGCTTGCCGGGAGCGCTTGCAGGGCAGGCCGGTGCAGTACATTGCG
GGAGAGGCGTTTTTTTACGGCTACCAGTTTTTCGTCGATGAGCGGGTGCTGATTCCTCGTCCGGAGACCGAACTGGTGCT
CGAACATGCGATGGAGCGGCTGGCCGCAAGTGGCCTCGATTCTGCCGATTCGCCGTCAATTCTCGACGTCGGCACCGGCA
GCGGTTGCATCGCCATCACGCTTGCCCTGCGCCTGCCTGGCGCTCGTGTTACCGCCGCCGATGTCTCCGCCGATGCGCTC
GATGTCGCCCGCCGCAATGCCGATGCGCACGGTGTGAGCGAACGCATCCGCTTCGTCGAAGCCGATGCGCTCAGCGCTTC
GTTTGCTGACGCCGTTGGCGGGCCGTTCGATCTGCTCGTCTCCAATCCGCCCTACATTCCAGAAGCGGAGTGGGCAACCC
TGCAGGAGGAGGTGAGGCGGTACGAACCGCGCCTGGCGTTGGTTGCACCTACCGGTTTCGAGTATTACCAGAGCATCGCC
GTCGCCGCGCCGTCGCTGCTTCGCAAGGGTGGCGTGCTCTGCTTCGAGCTTCATGCCGACGGCGCGGCTGAGGTCAGAAA
CCTGCTCGGTTCCAGCTTCGCCGACGTTCAGGTCATGCAGGATTACAACAAGCTCGATCGCGGGCTTTCGTGCATGGCAC
AGTAA

Upstream 100 bases:

>100_bases
AGAGCATTGAGCCGTAGTCATTTCTATTGGTTTTCTTTCATTGCCTCGCCCCTCCAAAATTCGTAAATATCCCCCAATTC
CATAACCCAAACCACAAGTC

Downstream 100 bases:

>100_bases
GCCCTTGCGGTTTATTCAAAGTTAATTTTCTGTATATTGTTTGCCGTTGCGGGCGATGGGCTTCTCAAGGCAACTATCAG
GGAGCTTTCGCTGTTAATGC

Product: HemK protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MPEEKVWSVVELLKTTIAFFAEKKIDEPRLSAELLLGHVLGLQRLQLYLDHERPLTLKELEAFRAACRERLQGRPVQYIA
GEAFFYGYQFFVDERVLIPRPETELVLEHAMERLAASGLDSADSPSILDVGTGSGCIAITLALRLPGARVTAADVSADAL
DVARRNADAHGVSERIRFVEADALSASFADAVGGPFDLLVSNPPYIPEAEWATLQEEVRRYEPRLALVAPTGFEYYQSIA
VAAPSLLRKGGVLCFELHADGAAEVRNLLGSSFADVQVMQDYNKLDRGLSCMAQ

Sequences:

>Translated_294_residues
MPEEKVWSVVELLKTTIAFFAEKKIDEPRLSAELLLGHVLGLQRLQLYLDHERPLTLKELEAFRAACRERLQGRPVQYIA
GEAFFYGYQFFVDERVLIPRPETELVLEHAMERLAASGLDSADSPSILDVGTGSGCIAITLALRLPGARVTAADVSADAL
DVARRNADAHGVSERIRFVEADALSASFADAVGGPFDLLVSNPPYIPEAEWATLQEEVRRYEPRLALVAPTGFEYYQSIA
VAAPSLLRKGGVLCFELHADGAAEVRNLLGSSFADVQVMQDYNKLDRGLSCMAQ
>Mature_293_residues
PEEKVWSVVELLKTTIAFFAEKKIDEPRLSAELLLGHVLGLQRLQLYLDHERPLTLKELEAFRAACRERLQGRPVQYIAG
EAFFYGYQFFVDERVLIPRPETELVLEHAMERLAASGLDSADSPSILDVGTGSGCIAITLALRLPGARVTAADVSADALD
VARRNADAHGVSERIRFVEADALSASFADAVGGPFDLLVSNPPYIPEAEWATLQEEVRRYEPRLALVAPTGFEYYQSIAV
AAPSLLRKGGVLCFELHADGAAEVRNLLGSSFADVQVMQDYNKLDRGLSCMAQ

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=256, Percent_Identity=36.328125, Blast_Score=131, Evalue=8e-31,
Organism=Escherichia coli, GI1787463, Length=265, Percent_Identity=34.3396226415094, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082085, Length=185, Percent_Identity=39.4594594594595, Blast_Score=112, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324266, Length=171, Percent_Identity=33.9181286549708, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24582226, Length=264, Percent_Identity=28.7878787878788, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32308; Mature: 32177

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEEKVWSVVELLKTTIAFFAEKKIDEPRLSAELLLGHVLGLQRLQLYLDHERPLTLKEL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EAFRAACRERLQGRPVQYIAGEAFFYGYQFFVDERVLIPRPETELVLEHAMERLAASGLD
HHHHHHHHHHHCCCCCHHHCCHHHHHHHHEEECCEEECCCCHHHHHHHHHHHHHHHCCCC
SADSPSILDVGTGSGCIAITLALRLPGARVTAADVSADALDVARRNADAHGVSERIRFVE
CCCCCCEEEECCCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHH
ADALSASFADAVGGPFDLLVSNPPYIPEAEWATLQEEVRRYEPRLALVAPTGFEYYQSIA
HHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
VAAPSLLRKGGVLCFELHADGAAEVRNLLGSSFADVQVMQDYNKLDRGLSCMAQ
HHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEEKVWSVVELLKTTIAFFAEKKIDEPRLSAELLLGHVLGLQRLQLYLDHERPLTLKEL
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
EAFRAACRERLQGRPVQYIAGEAFFYGYQFFVDERVLIPRPETELVLEHAMERLAASGLD
HHHHHHHHHHHCCCCCHHHCCHHHHHHHHEEECCEEECCCCHHHHHHHHHHHHHHHCCCC
SADSPSILDVGTGSGCIAITLALRLPGARVTAADVSADALDVARRNADAHGVSERIRFVE
CCCCCCEEEECCCCCCEEEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHH
ADALSASFADAVGGPFDLLVSNPPYIPEAEWATLQEEVRRYEPRLALVAPTGFEYYQSIA
HHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHH
VAAPSLLRKGGVLCFELHADGAAEVRNLLGSSFADVQVMQDYNKLDRGLSCMAQ
HHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]