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The map label for this gene is proB

Identifier: 21674278

GI number: 21674278

Start: 1366972

End: 1368057

Strand: Direct

Name: proB

Synonym: CT1457

Alternate gene names: 21674278

Gene position: 1366972-1368057 (Clockwise)

Preceding gene: 21674277

Following gene: 21674279

Centisome position: 63.43

GC content: 59.85

Gene sequence:

>1086_bases
ATGAGTCACCAACATCCGGTTTACAGGCGAATTGTCGTCAAGGTTGGAACCAACGTCATTACGGGGCGCAACGGCAAGCT
CGATCCAGCGATTCTCGACAGCCTGACCTCGCAGATCGCCGCACTCATGCAGGACGGCGTTGAAGTCATTCTCGTCACCT
CCGGCGCGGTCAGCGCCGGGCGCGGCATCGTGTCGCTCAGCGGTAATCTGACGCCGGTCGAGACGCGCCAGGTGCTGGCC
GCCACCGGGCAGATCCGGCTCATCAACGCCTACAATGACCGTTTCAAAAAACACGGCATAACCGGTGCTCAACTGCTCGT
CACCAAGGGCGATTTCCGCGACCGCCAGCACTACCTCAACATGCGCACCTGCTTCGAAGCGCTGCTTCAGCAGAAGATCG
TGCCGATCGTCAACGAGAACGACGCCGTGGCAATCACCGAGCTGATGTTCACCGACAACGACGAACTCTCCGGCCTCATC
GCATCGATGTTGCAGGTGGACGCGAACATCATTCTCTCGAACGTGGATGGCCTGTTCGATACACAGAGCGAAGGGAACCC
GGTGATCGAGGAGATCGATCCCGGCACGAAGAACTTCAGCCAGTACATCCGCCCCGGAAAATCGGAATTCGGGCGTGGCG
GCATGCTCACCAAGTGCAACATCGCGCACAAACTCTCGCGCCTCGGCATCACTGTGCATATCGCCAACGGCACCACTCCC
GGCATTCTACAGACCATCGCGCACGGCGGAAAAGCCGGCACGAAGTTCATCGCCCAGAAACCAAAGCAGAGCCGCAAGCG
CTGGGTCGCGCTCAGTGAAGGGCTTGAGAAGGGAGCCGTCATCATCAATCAGGGCGCCATCGACGCCCTGACCTCCGGCC
AGAGAGCCACAAGTCTTTTGCCTGTCGGCATCACCGGCGTCGAAGGCTCGTTCCAGCGCGGCGACATCATCCGCATCTGC
TCGACAGATGGCAAGGTCATCGGTTACGGCATGGCCTCCTGCACCGCAGAAAAAGCCCGCTCAGCGATGGGCCAGAAAGG
CCTCAAACCGGTCATCCACTACGATCACCTGTATCTGGTGCCGTAG

Upstream 100 bases:

>100_bases
AAGGATTGAACGCGCTCAAGATAATCTCCCTGATCCTGATCATCGCGGGCATTGCCGGATTGCATTTCAGTCAGGAACAC
ATGAAGTAAGGCGAACAGGC

Downstream 100 bases:

>100_bases
GAACCCTGCGTGCCTGCCCTTCCTCCGCCTCCGCAATATCCCCCAACCCGTTGCTCGCGCAGTAGAGCTCAGCGTTATCT
TGCAGCGTTCAGGTTTTGCT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSHQHPVYRRIVVKVGTNVITGRNGKLDPAILDSLTSQIAALMQDGVEVILVTSGAVSAGRGIVSLSGNLTPVETRQVLA
ATGQIRLINAYNDRFKKHGITGAQLLVTKGDFRDRQHYLNMRTCFEALLQQKIVPIVNENDAVAITELMFTDNDELSGLI
ASMLQVDANIILSNVDGLFDTQSEGNPVIEEIDPGTKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTTP
GILQTIAHGGKAGTKFIAQKPKQSRKRWVALSEGLEKGAVIINQGAIDALTSGQRATSLLPVGITGVEGSFQRGDIIRIC
STDGKVIGYGMASCTAEKARSAMGQKGLKPVIHYDHLYLVP

Sequences:

>Translated_361_residues
MSHQHPVYRRIVVKVGTNVITGRNGKLDPAILDSLTSQIAALMQDGVEVILVTSGAVSAGRGIVSLSGNLTPVETRQVLA
ATGQIRLINAYNDRFKKHGITGAQLLVTKGDFRDRQHYLNMRTCFEALLQQKIVPIVNENDAVAITELMFTDNDELSGLI
ASMLQVDANIILSNVDGLFDTQSEGNPVIEEIDPGTKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTTP
GILQTIAHGGKAGTKFIAQKPKQSRKRWVALSEGLEKGAVIINQGAIDALTSGQRATSLLPVGITGVEGSFQRGDIIRIC
STDGKVIGYGMASCTAEKARSAMGQKGLKPVIHYDHLYLVP
>Mature_360_residues
SHQHPVYRRIVVKVGTNVITGRNGKLDPAILDSLTSQIAALMQDGVEVILVTSGAVSAGRGIVSLSGNLTPVETRQVLAA
TGQIRLINAYNDRFKKHGITGAQLLVTKGDFRDRQHYLNMRTCFEALLQQKIVPIVNENDAVAITELMFTDNDELSGLIA
SMLQVDANIILSNVDGLFDTQSEGNPVIEEIDPGTKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTTPG
ILQTIAHGGKAGTKFIAQKPKQSRKRWVALSEGLEKGAVIINQGAIDALTSGQRATSLLPVGITGVEGSFQRGDIIRICS
TDGKVIGYGMASCTAEKARSAMGQKGLKPVIHYDHLYLVP

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=291, Percent_Identity=35.0515463917526, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI62912457, Length=289, Percent_Identity=35.2941176470588, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786437, Length=352, Percent_Identity=36.3636363636364, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17569969, Length=317, Percent_Identity=31.5457413249211, Blast_Score=120, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17569967, Length=317, Percent_Identity=31.5457413249211, Blast_Score=120, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=355, Percent_Identity=29.2957746478873, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=354, Percent_Identity=29.0960451977401, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21357643, Length=342, Percent_Identity=29.8245614035088, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_CHLTE (Q8KCG4)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662343.1
- ProteinModelPortal:   Q8KCG4
- GeneID:   1006004
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1457
- NMPDR:   fig|194439.1.peg.1437
- TIGR:   CT1457
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   CTEP194439:CT_1457-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 38779; Mature: 38648

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQHPVYRRIVVKVGTNVITGRNGKLDPAILDSLTSQIAALMQDGVEVILVTSGAVSAG
CCCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
RGIVSLSGNLTPVETRQVLAATGQIRLINAYNDRFKKHGITGAQLLVTKGDFRDRQHYLN
CEEEEECCCCCCHHHHHHHHHCCCEEEEECHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
MRTCFEALLQQKIVPIVNENDAVAITELMFTDNDELSGLIASMLQVDANIILSNVDGLFD
HHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCC
TQSEGNPVIEEIDPGTKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTTP
CCCCCCCCHHHCCCCHHHHHHHHCCCCHHCCCCCCEEEHHHHHHHHHCCEEEEECCCCCH
GILQTIAHGGKAGTKFIAQKPKQSRKRWVALSEGLEKGAVIINQGAIDALTSGQRATSLL
HHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCHHEEE
PVGITGVEGSFQRGDIIRICSTDGKVIGYGMASCTAEKARSAMGQKGLKPVIHYDHLYLV
EEEEECCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEECEEEEC
P
C
>Mature Secondary Structure 
SHQHPVYRRIVVKVGTNVITGRNGKLDPAILDSLTSQIAALMQDGVEVILVTSGAVSAG
CCCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
RGIVSLSGNLTPVETRQVLAATGQIRLINAYNDRFKKHGITGAQLLVTKGDFRDRQHYLN
CEEEEECCCCCCHHHHHHHHHCCCEEEEECHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
MRTCFEALLQQKIVPIVNENDAVAITELMFTDNDELSGLIASMLQVDANIILSNVDGLFD
HHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCC
TQSEGNPVIEEIDPGTKNFSQYIRPGKSEFGRGGMLTKCNIAHKLSRLGITVHIANGTTP
CCCCCCCCHHHCCCCHHHHHHHHCCCCHHCCCCCCEEEHHHHHHHHHCCEEEEECCCCCH
GILQTIAHGGKAGTKFIAQKPKQSRKRWVALSEGLEKGAVIINQGAIDALTSGQRATSLL
HHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCHHHHCCCCCHHEEE
PVGITGVEGSFQRGDIIRICSTDGKVIGYGMASCTAEKARSAMGQKGLKPVIHYDHLYLV
EEEEECCCCCCCCCCEEEEECCCCCEEECCHHHHHHHHHHHHHHHCCCCCEEEECEEEEC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901