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The map label for this gene is def

Identifier: 21674275

GI number: 21674275

Start: 1365277

End: 1365840

Strand: Reverse

Name: def

Synonym: CT1454

Alternate gene names: 21674275

Gene position: 1365840-1365277 (Counterclockwise)

Preceding gene: 21674281

Following gene: 21674274

Centisome position: 63.38

GC content: 57.98

Gene sequence:

>564_bases
ATGATTTTACCGATCAATACCTATAGCGACCCGGTACTCGCTATGAAAGCCAAGCCCCTGAAAGGGGTCGATTCAGCAAT
CGAGGAGTTGATTGCCGAGATGTTCGATACTATGTACAAGGCACCGGGCATCGGACTTGCTGCGCCGCAAGTGGGTCACT
CGCTACGCCTGGTAGTGGTGGATATTTCCACCATCAAGGAGTATGCCGATTTCAAGCCGATGGTGGTTATCAATCCCCGC
ATCGTTGCCGTTCGCGGGCGCAGCCTGATGGAGGAGGGGTGCCTCAGCGTGCCGGGCATTGCGGGTAACGTCGTCCGGCC
GTCGGCCATCACCCTGCACTACCGTGACGAGAAGTTTGAGGAGCACACCGCCGATTTTCACAGCATGATGGCCCGCGTGC
TTCAGCACGAGATCGACCATCTCGACGGCACGCTTTTCGTGGACCGCATGGACAAGCGTGATCGCCGCAAAATCCAGAAG
GAGCTTGACGCCATCGCCGAAGGACGGGTGAAGGCCGATTATCCGCTGGCCCGTGACGTTAACCGTGTCGAAGCGGAGGC
CTGA

Upstream 100 bases:

>100_bases
GCAACTATTTTGGAGTTAACAGGGTTATCTTCAGGAAAAGCTCTACCCTTTTGCTTTTGCAAGAGTTAACGGGCTATCCA
CAGTTAACGAGCAGTACGCA

Downstream 100 bases:

>100_bases
CCGGTGGGGTTGAGAGTTGTTTTCATGGGAACACCGGAGTTTGCCGTTCCTTCGCTGCGCCGTATCGCGGCCATGAAGCC
GCAGTTCGAGACGGTGCTGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MILPINTYSDPVLAMKAKPLKGVDSAIEELIAEMFDTMYKAPGIGLAAPQVGHSLRLVVVDISTIKEYADFKPMVVINPR
IVAVRGRSLMEEGCLSVPGIAGNVVRPSAITLHYRDEKFEEHTADFHSMMARVLQHEIDHLDGTLFVDRMDKRDRRKIQK
ELDAIAEGRVKADYPLARDVNRVEAEA

Sequences:

>Translated_187_residues
MILPINTYSDPVLAMKAKPLKGVDSAIEELIAEMFDTMYKAPGIGLAAPQVGHSLRLVVVDISTIKEYADFKPMVVINPR
IVAVRGRSLMEEGCLSVPGIAGNVVRPSAITLHYRDEKFEEHTADFHSMMARVLQHEIDHLDGTLFVDRMDKRDRRKIQK
ELDAIAEGRVKADYPLARDVNRVEAEA
>Mature_187_residues
MILPINTYSDPVLAMKAKPLKGVDSAIEELIAEMFDTMYKAPGIGLAAPQVGHSLRLVVVDISTIKEYADFKPMVVINPR
IVAVRGRSLMEEGCLSVPGIAGNVVRPSAITLHYRDEKFEEHTADFHSMMARVLQHEIDHLDGTLFVDRMDKRDRRKIQK
ELDAIAEGRVKADYPLARDVNRVEAEA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=165, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789682, Length=171, Percent_Identity=30.9941520467836, Blast_Score=95, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24645728, Length=178, Percent_Identity=32.0224719101124, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24645726, Length=190, Percent_Identity=27.3684210526316, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_CHLTE (Q8KCG7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662340.1
- ProteinModelPortal:   Q8KCG7
- SMR:   Q8KCG7
- GeneID:   1006001
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1454
- NMPDR:   fig|194439.1.peg.1434
- TIGR:   CT1454
- HOGENOM:   HBG665227
- OMA:   IIMHEND
- ProtClustDB:   PRK14598
- BioCyc:   CTEP194439:CT_1454-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 20910; Mature: 20910

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPINTYSDPVLAMKAKPLKGVDSAIEELIAEMFDTMYKAPGIGLAAPQVGHSLRLVVV
CEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEE
DISTIKEYADFKPMVVINPRIVAVRGRSLMEEGCLSVPGIAGNVVRPSAITLHYRDEKFE
EHHHHHHHHCCCCEEEECCEEEEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHH
EHTADFHSMMARVLQHEIDHLDGTLFVDRMDKRDRRKIQKELDAIAEGRVKADYPLARDV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHCH
NRVEAEA
HHHHCCC
>Mature Secondary Structure
MILPINTYSDPVLAMKAKPLKGVDSAIEELIAEMFDTMYKAPGIGLAAPQVGHSLRLVVV
CEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEE
DISTIKEYADFKPMVVINPRIVAVRGRSLMEEGCLSVPGIAGNVVRPSAITLHYRDEKFE
EHHHHHHHHCCCCEEEECCEEEEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCHHHH
EHTADFHSMMARVLQHEIDHLDGTLFVDRMDKRDRRKIQKELDAIAEGRVKADYPLARDV
HHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHCH
NRVEAEA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901