Click here to switch to the map view.

The map label for this gene is fmt

Identifier: 21674274

GI number: 21674274

Start: 1364329

End: 1365273

Strand: Reverse

Name: fmt

Synonym: CT1453

Alternate gene names: 21674274

Gene position: 1365273-1364329 (Counterclockwise)

Preceding gene: 21674275

Following gene: 21674273

Centisome position: 63.36

GC content: 61.16

Gene sequence:

>945_bases
GTGGGGTTGAGAGTTGTTTTCATGGGAACACCGGAGTTTGCCGTTCCTTCGCTGCGCCGTATCGCGGCCATGAAGCCGCA
GTTCGAGACGGTGCTGGTCGTGACAGGCTGCGACAAGCCTCGCCGCAGCAAAAACTCGCCGCCCGAGCCGACGCCGGTCA
AGCAGGCGGCGCTCGAACTGGGGCTTCCGGTTCTGGAGGCCGATGATGTCAGCAGTCATGAGTTTGCCTTGCAAGTCGCC
GCTGCAAGGCCGGACGTGATCGTGGTGGCAGCGTTCCGCGTTCTGCCACCGGAAGTGCTCGAGCTGCCGCCGCTCGGCAC
CTTCAACCTGCACGGCTCGCTGCTTCCCGCCTATCGCGGCGCGGCGCCGGTTAACTGGGCGATCATCAATGGCGACGCCG
AAACCGGTGTGACCACCTTTTTTCTTCAGAAGTCAGTCGATACCGGCAACATCATCACGATGGATCGCACGCCGATTGGT
CCGGACGAGAACGCCTTTGAGCTGCTCAAGCGATTGTCGGAAATTGGTGCAGGTACGGTCGAGCGCACGCTCACGATGAT
CGCCGATGGCGCGGTGATGCCTGAAAAGCAGGATGAGCGGTTCGCCACGAAAGCGCCGAAGCTGAACCGCGAGAACACCC
GCATCGACTGGAACCAGCCGGTGCAACGCCTGCATGACTTCATCCGCGGTCTCGCGCTGAAACCTGCCGCATGGACAACT
TTCGGCGGAAAGAGTCTGAAAATCTACAAGGCGAAGGCCTGTGCCATTGAAACAGCGCCGGATGAACCGGGGACGCTCCG
CATCGCGGACGGGCGACTGCTCGTGGCCGGAACTGACGGCTGGATCGAACTGCTCAGCGTGCAGGCCGAGGGCAAAAAAG
CGATGGACGGTGAACTGTTCGCTCGTGGACTTCGCGCCAGAAAGGAGATGCTTCGCTTTTTATGA

Upstream 100 bases:

>100_bases
CAAAATCCAGAAGGAGCTTGACGCCATCGCCGAAGGACGGGTGAAGGCCGATTATCCGCTGGCCCGTGACGTTAACCGTG
TCGAAGCGGAGGCCTGACCG

Downstream 100 bases:

>100_bases
AGGCTGCCGGGCGGTACGTCACAATTTTCTTCTTATATTTTTTGATTTTATTATTCTACGCCCGCCGCTCTTGCGGCGAA
GCGGTTGTGTAACCACTATT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MGLRVVFMGTPEFAVPSLRRIAAMKPQFETVLVVTGCDKPRRSKNSPPEPTPVKQAALELGLPVLEADDVSSHEFALQVA
AARPDVIVVAAFRVLPPEVLELPPLGTFNLHGSLLPAYRGAAPVNWAIINGDAETGVTTFFLQKSVDTGNIITMDRTPIG
PDENAFELLKRLSEIGAGTVERTLTMIADGAVMPEKQDERFATKAPKLNRENTRIDWNQPVQRLHDFIRGLALKPAAWTT
FGGKSLKIYKAKACAIETAPDEPGTLRIADGRLLVAGTDGWIELLSVQAEGKKAMDGELFARGLRARKEMLRFL

Sequences:

>Translated_314_residues
MGLRVVFMGTPEFAVPSLRRIAAMKPQFETVLVVTGCDKPRRSKNSPPEPTPVKQAALELGLPVLEADDVSSHEFALQVA
AARPDVIVVAAFRVLPPEVLELPPLGTFNLHGSLLPAYRGAAPVNWAIINGDAETGVTTFFLQKSVDTGNIITMDRTPIG
PDENAFELLKRLSEIGAGTVERTLTMIADGAVMPEKQDERFATKAPKLNRENTRIDWNQPVQRLHDFIRGLALKPAAWTT
FGGKSLKIYKAKACAIETAPDEPGTLRIADGRLLVAGTDGWIELLSVQAEGKKAMDGELFARGLRARKEMLRFL
>Mature_313_residues
GLRVVFMGTPEFAVPSLRRIAAMKPQFETVLVVTGCDKPRRSKNSPPEPTPVKQAALELGLPVLEADDVSSHEFALQVAA
ARPDVIVVAAFRVLPPEVLELPPLGTFNLHGSLLPAYRGAAPVNWAIINGDAETGVTTFFLQKSVDTGNIITMDRTPIGP
DENAFELLKRLSEIGAGTVERTLTMIADGAVMPEKQDERFATKAPKLNRENTRIDWNQPVQRLHDFIRGLALKPAAWTTF
GGKSLKIYKAKACAIETAPDEPGTLRIADGRLLVAGTDGWIELLSVQAEGKKAMDGELFARGLRARKEMLRFL

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=316, Percent_Identity=31.9620253164557, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI238814322, Length=196, Percent_Identity=29.0816326530612, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI21614513, Length=222, Percent_Identity=27.4774774774775, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1789683, Length=310, Percent_Identity=39.0322580645161, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1788589, Length=254, Percent_Identity=33.8582677165354, Blast_Score=144, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI133930964, Length=237, Percent_Identity=28.6919831223629, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI45550868, Length=317, Percent_Identity=27.1293375394322, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571984, Length=235, Percent_Identity=28.5106382978723, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585660, Length=203, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24582400, Length=147, Percent_Identity=32.6530612244898, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_CHLTE (Q8KCG8)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662339.1
- ProteinModelPortal:   Q8KCG8
- SMR:   Q8KCG8
- GeneID:   1006000
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1453
- NMPDR:   fig|194439.1.peg.1433
- TIGR:   CT1453
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   CLSK637772
- BioCyc:   CTEP194439:CT_1453-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34244; Mature: 34113

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLRVVFMGTPEFAVPSLRRIAAMKPQFETVLVVTGCDKPRRSKNSPPEPTPVKQAALEL
CCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
GLPVLEADDVSSHEFALQVAAARPDVIVVAAFRVLPPEVLELPPLGTFNLHGSLLPAYRG
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEEHHCCHHHHCCCCCCEECCCCCCCHHHCC
AAPVNWAIINGDAETGVTTFFLQKSVDTGNIITMDRTPIGPDENAFELLKRLSEIGAGTV
CCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCHH
ERTLTMIADGAVMPEKQDERFATKAPKLNRENTRIDWNQPVQRLHDFIRGLALKPAAWTT
HHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCEEEE
FGGKSLKIYKAKACAIETAPDEPGTLRIADGRLLVAGTDGWIELLSVQAEGKKAMDGELF
CCCCEEEEEEEEEEEEECCCCCCCEEEEECCEEEEEECCCEEEEEEEECCCCCCCCHHHH
ARGLRARKEMLRFL
HHHHHHHHHHHHCC
>Mature Secondary Structure 
GLRVVFMGTPEFAVPSLRRIAAMKPQFETVLVVTGCDKPRRSKNSPPEPTPVKQAALEL
CEEEEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
GLPVLEADDVSSHEFALQVAAARPDVIVVAAFRVLPPEVLELPPLGTFNLHGSLLPAYRG
CCCEEECCCCCCCEEEEEEECCCCCEEEEEEEHHCCHHHHCCCCCCEECCCCCCCHHHCC
AAPVNWAIINGDAETGVTTFFLQKSVDTGNIITMDRTPIGPDENAFELLKRLSEIGAGTV
CCCCEEEEECCCCCCCEEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCHH
ERTLTMIADGAVMPEKQDERFATKAPKLNRENTRIDWNQPVQRLHDFIRGLALKPAAWTT
HHHHHHHCCCCCCCCCCCCHHHHCCCCCCCCCCEECHHHHHHHHHHHHHCCCCCCCEEEE
FGGKSLKIYKAKACAIETAPDEPGTLRIADGRLLVAGTDGWIELLSVQAEGKKAMDGELF
CCCCEEEEEEEEEEEEECCCCCCCEEEEECCEEEEEECCCEEEEEEEECCCCCCCCHHHH
ARGLRARKEMLRFL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901