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The map label for this gene is hemC

Identifier: 21674248

GI number: 21674248

Start: 1339848

End: 1340786

Strand: Direct

Name: hemC

Synonym: CT1427

Alternate gene names: 21674248

Gene position: 1339848-1340786 (Clockwise)

Preceding gene: 21674247

Following gene: 21674249

Centisome position: 62.18

GC content: 55.59

Gene sequence:

>939_bases
TTGAAAAAAGAGCTTATCATCGGCACTCGGTCCAGCCCTCTTGCCCTGTGGCAGGCTGAATTCACCAAAGCAGAACTGTC
CAGACACTTTCCTGAACTGAATATCACGCTCAAACTGGTCAAAACGACCGGTGATGTGCTGCTCGATTCCCCTCTTTCCA
AGATCGGAGACATGGGCCTGTTCACCAAAGACATTGAAAAGCACCTGCTTGCAAAAGAGATCGACCTGGCAGTGCATAGC
CTGAAGGACGTTCCAACCGGCACGCCTGAGGGTCTGGTCATCTCCTCGTTCACCAAGCGCGAAGATACCCGCGACGTTAT
TATCTCAAAATCCGGCAAGGGCCTGAAAGACCTTCCGCCGAATGCACGCATGGCCACCAGCTCCCTGCGCAGAATGTCGC
AGCTTCTGAGCATGAGGCCTGACTTGCAGATTCTCGACATCAGGGGCAACCTCAACACCCGCTTCAAGAAGTTCGACGAC
GGCGAATTCGACGCCATGATGCTGGCCTATGCTGGCGTGTATCGCCTTGAGTTCAGCGACCGCATCTCGGAAATCCTGCC
GCACGACGTGATGCTTCCGGCAGTCGGTCAGGGTGCACTCGGCATCGAGACCCGCACGGATGACGCTGAAACCATAGAGA
TCGTCCGCGTCATGAACGACAGCAACACTGAAATCTGCTGCCGTGCAGAGCGTGCGCTTCTCAGGCACCTCCAGGGTGGC
TGCCAGATTCCGATCGGCTGTTATGGCTCATATATCAGCGGCACCTTGAAGCTTCTAGCTTTTGTTGGCTCTGTAGACGG
TAAAACCGCCCTGCGCAATGAGCTGACCAAGCCGGTAAACACCCCGGAAGAGGCCGAGGCTGTCGGTATTGAGCTGGCTG
AAGTTCTGCTTTCGATGGGCGCGGAGAAAATTCTCGCCGACATCCGCAAAACCCGCTGA

Upstream 100 bases:

>100_bases
CCGCCGACAATATCCCCAGCAAGGTCAACCTCGTCAGGAACATCTTCGATCTTGAAGAACCAAACCAGTCACTCCAATAA
AATCAATCAGTCATCGAATT

Downstream 100 bases:

>100_bases
TGAAAACCGTTCTCGTTACACGCCCGAAGCATCAGGCCGAGCCTTTTGTGAGGGAACTTGCGCAGTACGGCCTGGACTCG
GTTGTCTTTCCAACGATCGA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MKKELIIGTRSSPLALWQAEFTKAELSRHFPELNITLKLVKTTGDVLLDSPLSKIGDMGLFTKDIEKHLLAKEIDLAVHS
LKDVPTGTPEGLVISSFTKREDTRDVIISKSGKGLKDLPPNARMATSSLRRMSQLLSMRPDLQILDIRGNLNTRFKKFDD
GEFDAMMLAYAGVYRLEFSDRISEILPHDVMLPAVGQGALGIETRTDDAETIEIVRVMNDSNTEICCRAERALLRHLQGG
CQIPIGCYGSYISGTLKLLAFVGSVDGKTALRNELTKPVNTPEEAEAVGIELAEVLLSMGAEKILADIRKTR

Sequences:

>Translated_312_residues
MKKELIIGTRSSPLALWQAEFTKAELSRHFPELNITLKLVKTTGDVLLDSPLSKIGDMGLFTKDIEKHLLAKEIDLAVHS
LKDVPTGTPEGLVISSFTKREDTRDVIISKSGKGLKDLPPNARMATSSLRRMSQLLSMRPDLQILDIRGNLNTRFKKFDD
GEFDAMMLAYAGVYRLEFSDRISEILPHDVMLPAVGQGALGIETRTDDAETIEIVRVMNDSNTEICCRAERALLRHLQGG
CQIPIGCYGSYISGTLKLLAFVGSVDGKTALRNELTKPVNTPEEAEAVGIELAEVLLSMGAEKILADIRKTR
>Mature_312_residues
MKKELIIGTRSSPLALWQAEFTKAELSRHFPELNITLKLVKTTGDVLLDSPLSKIGDMGLFTKDIEKHLLAKEIDLAVHS
LKDVPTGTPEGLVISSFTKREDTRDVIISKSGKGLKDLPPNARMATSSLRRMSQLLSMRPDLQILDIRGNLNTRFKKFDD
GEFDAMMLAYAGVYRLEFSDRISEILPHDVMLPAVGQGALGIETRTDDAETIEIVRVMNDSNTEICCRAERALLRHLQGG
CQIPIGCYGSYISGTLKLLAFVGSVDGKTALRNELTKPVNTPEEAEAVGIELAEVLLSMGAEKILADIRKTR

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=334, Percent_Identity=39.2215568862275, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI66933009, Length=334, Percent_Identity=39.2215568862275, Blast_Score=229, Evalue=2e-60,
Organism=Escherichia coli, GI48994974, Length=304, Percent_Identity=47.6973684210526, Blast_Score=275, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319996, Length=320, Percent_Identity=39.375, Blast_Score=208, Evalue=9e-55,
Organism=Drosophila melanogaster, GI20130425, Length=293, Percent_Identity=37.542662116041, Blast_Score=190, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_CHLTE (Q8KCJ4)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662313.1
- ProteinModelPortal:   Q8KCJ4
- SMR:   Q8KCJ4
- GeneID:   1005971
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1427
- NMPDR:   fig|194439.1.peg.1407
- TIGR:   CT1427
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   CTEP194439:CT_1427-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34394; Mature: 34394

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKELIIGTRSSPLALWQAEFTKAELSRHFPELNITLKLVKTTGDVLLDSPLSKIGDMGL
CCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHCCHHHHHHCCCC
FTKDIEKHLLAKEIDLAVHSLKDVPTGTPEGLVISSFTKREDTRDVIISKSGKGLKDLPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHCCCCCCCEEEECCCCCCCCCCC
NARMATSSLRRMSQLLSMRPDLQILDIRGNLNTRFKKFDDGEFDAMMLAYAGVYRLEFSD
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHEEEHHH
RISEILPHDVMLPAVGQGALGIETRTDDAETIEIVRVMNDSNTEICCRAERALLRHLQGG
HHHHHCCCCEECCCCCCCCEECCCCCCCHHHEEEEEEECCCCCCEEHHHHHHHHHHHCCC
CQIPIGCYGSYISGTLKLLAFVGSVDGKTALRNELTKPVNTPEEAEAVGIELAEVLLSMG
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
AEKILADIRKTR
HHHHHHHHHHCC
>Mature Secondary Structure
MKKELIIGTRSSPLALWQAEFTKAELSRHFPELNITLKLVKTTGDVLLDSPLSKIGDMGL
CCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHCCHHHHHHCCCC
FTKDIEKHLLAKEIDLAVHSLKDVPTGTPEGLVISSFTKREDTRDVIISKSGKGLKDLPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHCCCCCCCEEEECCCCCCCCCCC
NARMATSSLRRMSQLLSMRPDLQILDIRGNLNTRFKKFDDGEFDAMMLAYAGVYRLEFSD
CCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHEEEHHH
RISEILPHDVMLPAVGQGALGIETRTDDAETIEIVRVMNDSNTEICCRAERALLRHLQGG
HHHHHCCCCEECCCCCCCCEECCCCCCCHHHEEEEEEECCCCCCEEHHHHHHHHHHHCCC
CQIPIGCYGSYISGTLKLLAFVGSVDGKTALRNELTKPVNTPEEAEAVGIELAEVLLSMG
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
AEKILADIRKTR
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901