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The map label for this gene is appA [H]

Identifier: 21674241

GI number: 21674241

Start: 1330501

End: 1332234

Strand: Direct

Name: appA [H]

Synonym: CT1420

Alternate gene names: 21674241

Gene position: 1330501-1332234 (Clockwise)

Preceding gene: 21674240

Following gene: 21674242

Centisome position: 61.74

GC content: 58.3

Gene sequence:

>1734_bases
TTGACGGCGGGCCAACCCATCAATAGAATTTCAGACCTGACCGCCATGCACAAATCATTGCTGAGCATCGCCGCTCTTTT
GCTAGTGACGCTGTTCGCGGGCTGTGGCCCGAAATCGCAGAAATCCCGGCCCGACACCATCGTGGTTGCCGTCTCAGCCG
ATTTCGACCACCTCAACCCGCTGCTCATCCAGATGTCGCTCGCCCGCGAGGTGTGTACGATGATCTATCCCCAGCTTGTC
AAGCCGTCGTTCGACGAAAAGAGCGGCGCGATCAGCTACCAGCCGAACGCCGCCGAGCGCTGGGAGTTCTCACCGGACGG
GCGAAACGTGACGTTCCACCTGAACAGCAAGGCGGTCTGGGAGGATGGCAAGCCGCTCACCTCGAAGGACTTCAGTTTTT
CCTACCGCCTGTATGCCGACCCAAACGTGGCAAGCAGTCGTCAGGATTACCTGTACGACCTGCTGCTCAAGCCTGATGGC
TCGGTCGATTTCGACAAAGCAGTCGAGACTCCGGATGACAAGACGCTGGTGCTGCACTTTAACAAGCCGATGGCCGAAAA
CATCGTGCTCGACCACTTCAACGACCTGATGCCAGTCGCCGAGCACCTCTTCCGAGACATCAAGCCGCAACAGATCCGCC
AACAGGCGGCCACATTGCCAATCATGGGTGCAGGCCCGTTCAAAGTAAAGGAGTGGCAGCGGCAATCGAAGCTAGTGCTC
GAATCGAATCCAACCTCGGTGCTGCCACGTCCGGCAGCCTCGCCGGCGCTCACCTTCATGGTGGTGCCGGAGTACACAAC
GCGGCTGGCGATGCTCAAATCGGGCCAGATCGACGCGCTCGTATCGGCAGGCGGCATCAACCCGAAAGACGCCGCGGAGC
TGGCCAAAAGCAACCCGGAAATTGCGATTATCCCGGTGGCCGACCGCTACTTTGATAGCGTCGTCTGGCTGAACATCGAC
GGCGAAGCGTGGCGCAACGGCAAGAAGATCAAGCCGAACCGCTTTTTCGGTGACCGCCGCGTGCGGCAAGCGATGACCTA
TGCCATCGACCGGCAGGCGATCATCGACGGCTTCATGGGGCCAAAGCATGCTACCATCGTCAACACCACGCTCTCGCCCG
CCTACACCTCGATTATTGACACATCGCTGCCCGCGTACGCCTACAATCCCGACAAGGCGCTCGCCCTTCTCAAAGAGGCT
GGCTGGACGCCGGGGCCGGACGGCATTTTGCAGAAAAACGGCCAGAAGTTCTCGTTCGAACTCGCCGCGCCCACCGGCAA
CCCGAGGCGCAACTACGCAGCAACGATCATCCAGCAGAACCTCCGCAAAATCGGCATCGACTGCCGCCTGCGCTTCGACG
AGTCTCTCATGTTCAACAAAAACCAGAACGAGTACCGCTACGACGCCGCCTTGTCGGGACTGGCGGCTGAAACGCTCCCC
TTCCAGCTCGTCATCTGGGGATCGGACTTCGAGAAAAAACCCTTCAACTCCTCAGCCTTCCAGAACGCCGAACTCGACCG
CGTCGTCGCCAGACTCACCGGCCCGCTTCCGCAAACCGAACAGGCAAAACTCTGGAAAGAGTACCAGCAAATTCTCGCCA
ACGAACAGCCGCGAACCTTTTTGTATTATTACGATGAACTTGAGGGATTCAACAAGCGAGTGGAGAATGTCAATTTGAGC
CTGCTGGCGACGCTGGGAAATATGTATGAGTGGAAGGTTGGGAAAAAGAAATGA

Upstream 100 bases:

>100_bases
GGGCACTCGGCGAGCAGATCGGCGTCATGTACGGCGAGGGGTTCCGCCTGCCGACGGCACTCGGCATCGATCACTTCACG
AGCGTTTTTCCGCCCGGCGT

Downstream 100 bases:

>100_bases
CTTTCGAGCGACCACAGGGTTACCCCGCGAGCTTCCCCATCTTGGTTTTGAGCTCTTCGCAGAATATCGTGGGTAAATGG
TGAATATGGGTCAATCGACT

Product: peptide ABC transporter, periplasmic peptide-binding protein, putative

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MTAGQPINRISDLTAMHKSLLSIAALLLVTLFAGCGPKSQKSRPDTIVVAVSADFDHLNPLLIQMSLAREVCTMIYPQLV
KPSFDEKSGAISYQPNAAERWEFSPDGRNVTFHLNSKAVWEDGKPLTSKDFSFSYRLYADPNVASSRQDYLYDLLLKPDG
SVDFDKAVETPDDKTLVLHFNKPMAENIVLDHFNDLMPVAEHLFRDIKPQQIRQQAATLPIMGAGPFKVKEWQRQSKLVL
ESNPTSVLPRPAASPALTFMVVPEYTTRLAMLKSGQIDALVSAGGINPKDAAELAKSNPEIAIIPVADRYFDSVVWLNID
GEAWRNGKKIKPNRFFGDRRVRQAMTYAIDRQAIIDGFMGPKHATIVNTTLSPAYTSIIDTSLPAYAYNPDKALALLKEA
GWTPGPDGILQKNGQKFSFELAAPTGNPRRNYAATIIQQNLRKIGIDCRLRFDESLMFNKNQNEYRYDAALSGLAAETLP
FQLVIWGSDFEKKPFNSSAFQNAELDRVVARLTGPLPQTEQAKLWKEYQQILANEQPRTFLYYYDELEGFNKRVENVNLS
LLATLGNMYEWKVGKKK

Sequences:

>Translated_577_residues
MTAGQPINRISDLTAMHKSLLSIAALLLVTLFAGCGPKSQKSRPDTIVVAVSADFDHLNPLLIQMSLAREVCTMIYPQLV
KPSFDEKSGAISYQPNAAERWEFSPDGRNVTFHLNSKAVWEDGKPLTSKDFSFSYRLYADPNVASSRQDYLYDLLLKPDG
SVDFDKAVETPDDKTLVLHFNKPMAENIVLDHFNDLMPVAEHLFRDIKPQQIRQQAATLPIMGAGPFKVKEWQRQSKLVL
ESNPTSVLPRPAASPALTFMVVPEYTTRLAMLKSGQIDALVSAGGINPKDAAELAKSNPEIAIIPVADRYFDSVVWLNID
GEAWRNGKKIKPNRFFGDRRVRQAMTYAIDRQAIIDGFMGPKHATIVNTTLSPAYTSIIDTSLPAYAYNPDKALALLKEA
GWTPGPDGILQKNGQKFSFELAAPTGNPRRNYAATIIQQNLRKIGIDCRLRFDESLMFNKNQNEYRYDAALSGLAAETLP
FQLVIWGSDFEKKPFNSSAFQNAELDRVVARLTGPLPQTEQAKLWKEYQQILANEQPRTFLYYYDELEGFNKRVENVNLS
LLATLGNMYEWKVGKKK
>Mature_576_residues
TAGQPINRISDLTAMHKSLLSIAALLLVTLFAGCGPKSQKSRPDTIVVAVSADFDHLNPLLIQMSLAREVCTMIYPQLVK
PSFDEKSGAISYQPNAAERWEFSPDGRNVTFHLNSKAVWEDGKPLTSKDFSFSYRLYADPNVASSRQDYLYDLLLKPDGS
VDFDKAVETPDDKTLVLHFNKPMAENIVLDHFNDLMPVAEHLFRDIKPQQIRQQAATLPIMGAGPFKVKEWQRQSKLVLE
SNPTSVLPRPAASPALTFMVVPEYTTRLAMLKSGQIDALVSAGGINPKDAAELAKSNPEIAIIPVADRYFDSVVWLNIDG
EAWRNGKKIKPNRFFGDRRVRQAMTYAIDRQAIIDGFMGPKHATIVNTTLSPAYTSIIDTSLPAYAYNPDKALALLKEAG
WTPGPDGILQKNGQKFSFELAAPTGNPRRNYAATIIQQNLRKIGIDCRLRFDESLMFNKNQNEYRYDAALSGLAAETLPF
QLVIWGSDFEKKPFNSSAFQNAELDRVVARLTGPLPQTEQAKLWKEYQQILANEQPRTFLYYYDELEGFNKRVENVNLSL
LATLGNMYEWKVGKKK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789887, Length=487, Percent_Identity=26.0780287474333, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789966, Length=528, Percent_Identity=22.5378787878788, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787495, Length=464, Percent_Identity=22.6293103448276, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787762, Length=549, Percent_Identity=22.040072859745, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789397, Length=515, Percent_Identity=22.9126213592233, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64708; Mature: 64576

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGQPINRISDLTAMHKSLLSIAALLLVTLFAGCGPKSQKSRPDTIVVAVSADFDHLNP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCH
LLIQMSLAREVCTMIYPQLVKPSFDEKSGAISYQPNAAERWEFSPDGRNVTFHLNSKAVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCEEE
EDGKPLTSKDFSFSYRLYADPNVASSRQDYLYDLLLKPDGSVDFDKAVETPDDKTLVLHF
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCCCCHHHHCCCCCCCEEEEEE
NKPMAENIVLDHFNDLMPVAEHLFRDIKPQQIRQQAATLPIMGAGPFKVKEWQRQSKLVL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCHHHHHHHHCCEEE
ESNPTSVLPRPAASPALTFMVVPEYTTRLAMLKSGQIDALVSAGGINPKDAAELAKSNPE
ECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCC
IAIIPVADRYFDSVVWLNIDGEAWRNGKKIKPNRFFGDRRVRQAMTYAIDRQAIIDGFMG
EEEEECCHHHHCEEEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PKHATIVNTTLSPAYTSIIDTSLPAYAYNPDKALALLKEAGWTPGPDGILQKNGQKFSFE
CCCEEEEECCCCHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEEEEE
LAAPTGNPRRNYAATIIQQNLRKIGIDCRLRFDESLMFNKNQNEYRYDAALSGLAAETLP
EECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCEEHHHHHHCCHHCCCC
FQLVIWGSDFEKKPFNSSAFQNAELDRVVARLTGPLPQTEQAKLWKEYQQILANEQPRTF
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
LYYYDELEGFNKRVENVNLSLLATLGNMYEWKVGKKK
EEEEHHHHHHHHHHHCCCEEEEEHHCCCEEECCCCCC
>Mature Secondary Structure 
TAGQPINRISDLTAMHKSLLSIAALLLVTLFAGCGPKSQKSRPDTIVVAVSADFDHLNP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCH
LLIQMSLAREVCTMIYPQLVKPSFDEKSGAISYQPNAAERWEFSPDGRNVTFHLNSKAVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCEEE
EDGKPLTSKDFSFSYRLYADPNVASSRQDYLYDLLLKPDGSVDFDKAVETPDDKTLVLHF
CCCCCCCCCCCCEEEEEEECCCCCCCCHHHEEEEEECCCCCCCHHHHCCCCCCCEEEEEE
NKPMAENIVLDHFNDLMPVAEHLFRDIKPQQIRQQAATLPIMGAGPFKVKEWQRQSKLVL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCHHHHHHHHCCEEE
ESNPTSVLPRPAASPALTFMVVPEYTTRLAMLKSGQIDALVSAGGINPKDAAELAKSNPE
ECCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCC
IAIIPVADRYFDSVVWLNIDGEAWRNGKKIKPNRFFGDRRVRQAMTYAIDRQAIIDGFMG
EEEEECCHHHHCEEEEEEECCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PKHATIVNTTLSPAYTSIIDTSLPAYAYNPDKALALLKEAGWTPGPDGILQKNGQKFSFE
CCCEEEEECCCCHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEEEEE
LAAPTGNPRRNYAATIIQQNLRKIGIDCRLRFDESLMFNKNQNEYRYDAALSGLAAETLP
EECCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCEEHHHHHHCCHHCCCC
FQLVIWGSDFEKKPFNSSAFQNAELDRVVARLTGPLPQTEQAKLWKEYQQILANEQPRTF
EEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEE
LYYYDELEGFNKRVENVNLSLLATLGNMYEWKVGKKK
EEEEHHHHHHHHHHHCCCEEEEEHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]