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The map label for this gene is lpd-1

Identifier: 21674121

GI number: 21674121

Start: 1219268

End: 1220677

Strand: Reverse

Name: lpd-1

Synonym: CT1298

Alternate gene names: 21674121

Gene position: 1220677-1219268 (Counterclockwise)

Preceding gene: 21674125

Following gene: 21674120

Centisome position: 56.65

GC content: 63.33

Gene sequence:

>1410_bases
ATGCAGCAGGCAGATACTCTCGCAGCGCAATTCGATGTCGCCGTCATCGGTTCCGGCCCCGGCGGTTACGAGGCGGCCAT
TCATGCCGCGCGTTACGGCCTGAAGACCTGCATTGTCGAAAAGGCGGTGCTTGGCGGTGTTTGCGTCAACTGGGGCTGCA
TTCCGACCAAAGCGCTGCTTCGGAGTGCAGAGGTGTTTGATCTGGCAAAGAATCCAGAGACTTTTGGCGTCAATGTCGGC
AACGTATCGTTCGATCTCGCGCAGGCGGTCAAGCGCAGCCGGAATGTGGCGCTGAAAAGCTCGAAGGGCGTGGCGTATCT
GCTCAAGAAGGCTGCTGTTGAGGTGTTGGCGGGCGAGGCGGTGCTGACCGGCGGCGCAGGCGTGATGGTCACCATGCCGG
ACGGTTCTGTGCGCATGCTCGGAGCGAAAAACATCATTGTTGCGACGGGATCGACGCCGCGCGTGATTCCGGGGCTGGAG
CCGGACGGCAAAAAGATCATCACCAGCCGCGAGGCGCTGATTCTGAAAGAGGTGCCGAAGTCGATGATCGTGGTCGGCGG
TGGCGCGATTGGCGTCGAGATGGCCTGGTTCTACGCCAAGGCGGGCTCGAAGGTGACGATTGTCGAGCTGATGCCGCGCA
TGCTACCCGCCGAAGAGGCGGAGGTTTCCGAGGCGCTGAAGCGCTCGTTCGAGAAGGCGGGCATCACGGTGCACTGTGGC
GCGAAGCTCGATAATGTTGCTGTCAGCGAGTCCGGCGTATCCGCCGAACTGGTCGTCGAAGGCTCGGCGCCGCAGACGCT
CAATGCGTCGTGCCTGCTTGTGGCTGTCGGCGTGACCGGCGCGATCGATGGGCTCGGCCTCGATGCAGTGGGCGTCGAAA
CGGAGCGCGGATTCATTCGTACCGACGGGCAGTGCCGTACCTCCGCGCCGGGTATCTATGCTATCGGCGACGTTCGAGGC
GGGATGCTGCTGGCGCACAAGGCGTCGGCGGAGGCGGCCATCGCGGTCGAAGCCATTGCCGGAAAATCGCCCGAGCCGCT
TTCGGAGCCGCTTATTCCGCGCTGTGTCTATGCCCAGCCGTCGGTGGCTTCGGTTGGCCTGACCGAGGAGGCCGCGGTCA
ACGCCGGGTATCAGGTCGCGGTAGGCCGCTCGCAGTTCGCCGCCTCGGGCAAAGCCAACGCCTATGGGCAGCTCGAAGGC
TTCGTCAAGCTGGTGTTTGACGCTGCAACCGGCAAGATGCTCGGCGGCCATCTCATCGGCCACGATGCGGTTGAGCTGAT
TGGCGAACTTGGACTGGCGTGCCGCTATGGCGTGACGGCGGGAGGCCTCGTGAATACGGTTCACGCCCATCCGACCCTGT
CGGAAACGGTCAGGGAGGCGGCGTTTGATGCGCTTCAAAGCATGGGGTAA

Upstream 100 bases:

>100_bases
TATGTGAAAGTTATTATAGTCAAAGAATCGTCTCGTGCAACCGTGAATGTCCATCGCTGCCGATTCAGGGGGTGATGCGC
TTCACACAACCAGTTACGTT

Downstream 100 bases:

>100_bases
AACCTGGTTTTTTGTGAGTTTTTTATCGTGTCGCATTCGTGAAAAGCGTTTGTTATCTCTATGCTAAAAGCATAAATTCA
AAGTTCATTATTTGATTTTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MQQADTLAAQFDVAVIGSGPGGYEAAIHAARYGLKTCIVEKAVLGGVCVNWGCIPTKALLRSAEVFDLAKNPETFGVNVG
NVSFDLAQAVKRSRNVALKSSKGVAYLLKKAAVEVLAGEAVLTGGAGVMVTMPDGSVRMLGAKNIIVATGSTPRVIPGLE
PDGKKIITSREALILKEVPKSMIVVGGGAIGVEMAWFYAKAGSKVTIVELMPRMLPAEEAEVSEALKRSFEKAGITVHCG
AKLDNVAVSESGVSAELVVEGSAPQTLNASCLLVAVGVTGAIDGLGLDAVGVETERGFIRTDGQCRTSAPGIYAIGDVRG
GMLLAHKASAEAAIAVEAIAGKSPEPLSEPLIPRCVYAQPSVASVGLTEEAAVNAGYQVAVGRSQFAASGKANAYGQLEG
FVKLVFDAATGKMLGGHLIGHDAVELIGELGLACRYGVTAGGLVNTVHAHPTLSETVREAAFDALQSMG

Sequences:

>Translated_469_residues
MQQADTLAAQFDVAVIGSGPGGYEAAIHAARYGLKTCIVEKAVLGGVCVNWGCIPTKALLRSAEVFDLAKNPETFGVNVG
NVSFDLAQAVKRSRNVALKSSKGVAYLLKKAAVEVLAGEAVLTGGAGVMVTMPDGSVRMLGAKNIIVATGSTPRVIPGLE
PDGKKIITSREALILKEVPKSMIVVGGGAIGVEMAWFYAKAGSKVTIVELMPRMLPAEEAEVSEALKRSFEKAGITVHCG
AKLDNVAVSESGVSAELVVEGSAPQTLNASCLLVAVGVTGAIDGLGLDAVGVETERGFIRTDGQCRTSAPGIYAIGDVRG
GMLLAHKASAEAAIAVEAIAGKSPEPLSEPLIPRCVYAQPSVASVGLTEEAAVNAGYQVAVGRSQFAASGKANAYGQLEG
FVKLVFDAATGKMLGGHLIGHDAVELIGELGLACRYGVTAGGLVNTVHAHPTLSETVREAAFDALQSMG
>Mature_469_residues
MQQADTLAAQFDVAVIGSGPGGYEAAIHAARYGLKTCIVEKAVLGGVCVNWGCIPTKALLRSAEVFDLAKNPETFGVNVG
NVSFDLAQAVKRSRNVALKSSKGVAYLLKKAAVEVLAGEAVLTGGAGVMVTMPDGSVRMLGAKNIIVATGSTPRVIPGLE
PDGKKIITSREALILKEVPKSMIVVGGGAIGVEMAWFYAKAGSKVTIVELMPRMLPAEEAEVSEALKRSFEKAGITVHCG
AKLDNVAVSESGVSAELVVEGSAPQTLNASCLLVAVGVTGAIDGLGLDAVGVETERGFIRTDGQCRTSAPGIYAIGDVRG
GMLLAHKASAEAAIAVEAIAGKSPEPLSEPLIPRCVYAQPSVASVGLTEEAAVNAGYQVAVGRSQFAASGKANAYGQLEG
FVKLVFDAATGKMLGGHLIGHDAVELIGELGLACRYGVTAGGLVNTVHAHPTLSETVREAAFDALQSMG

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=36.8763557483731, Blast_Score=273, Evalue=3e-73,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=29.5896328293737, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=26.8558951965066, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=23.5042735042735, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=23.5042735042735, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=23.5042735042735, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=23.5042735042735, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=23.5042735042735, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI291045266, Length=481, Percent_Identity=25.1559251559252, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI291045268, Length=473, Percent_Identity=24.3128964059197, Blast_Score=80, Evalue=6e-15,
Organism=Escherichia coli, GI1786307, Length=457, Percent_Identity=38.074398249453, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=29.8507462686567, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=27.7899343544858, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789915, Length=434, Percent_Identity=29.4930875576037, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788892, Length=185, Percent_Identity=29.7297297297297, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=38.695652173913, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71983429, Length=457, Percent_Identity=28.6652078774617, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=457, Percent_Identity=28.6652078774617, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=25.2631578947368, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=439, Percent_Identity=21.6400911161731, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=238, Percent_Identity=25.6302521008403, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=38.1856540084388, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=30.7368421052632, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=27.1367521367521, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.2625820568928, Blast_Score=289, Evalue=3e-78,
Organism=Drosophila melanogaster, GI17737741, Length=473, Percent_Identity=27.061310782241, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24640549, Length=471, Percent_Identity=26.7515923566879, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24640553, Length=473, Percent_Identity=26.6384778012685, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=26.7515923566879, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): DLDH_CHLTE (Q8KCW2)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662186.1
- ProteinModelPortal:   Q8KCW2
- SMR:   Q8KCW2
- GeneID:   1006658
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1298
- TIGR:   CT1298
- HOGENOM:   HBG515043
- OMA:   EMAWFYA
- ProtClustDB:   CLSK637692
- BioCyc:   CTEP194439:CT_1298-MONOMER
- BRENDA:   1.8.1.4
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327
- Gene3D:   G3DSA:3.30.390.30
- PANTHER:   PTHR22912:SF20
- PRINTS:   PR00368
- TIGRFAMs:   TIGR01350

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim; SSF55424 FAD/NAD-linked_reductase_dimer

EC number: =1.8.1.4

Molecular weight: Translated: 48008; Mature: 48008

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: ACT_SITE 450-450 BINDING 57-57 BINDING 120-120 BINDING 209-209 BINDING 317-317 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQADTLAAQFDVAVIGSGPGGYEAAIHAARYGLKTCIVEKAVLGGVCVNWGCIPTKALL
CCCHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHH
RSAEVFDLAKNPETFGVNVGNVSFDLAQAVKRSRNVALKSSKGVAYLLKKAAVEVLAGEA
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCE
VLTGGAGVMVTMPDGSVRMLGAKNIIVATGSTPRVIPGLEPDGKKIITSREALILKEVPK
EEECCCEEEEECCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHCCC
SMIVVGGGAIGVEMAWFYAKAGSKVTIVELMPRMLPAEEAEVSEALKRSFEKAGITVHCG
EEEEECCCCHHHEEEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEC
AKLDNVAVSESGVSAELVVEGSAPQTLNASCLLVAVGVTGAIDGLGLDAVGVETERGFIR
CCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECHHCCCCCCCCEECCCCCCCEEE
TDGQCRTSAPGIYAIGDVRGGMLLAHKASAEAAIAVEAIAGKSPEPLSEPLIPRCVYAQP
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCC
SVASVGLTEEAAVNAGYQVAVGRSQFAASGKANAYGQLEGFVKLVFDAATGKMLGGHLIG
CCCCCCCCHHHHCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHEEEHHCCCCHHCCEEEC
HDAVELIGELGLACRYGVTAGGLVNTVHAHPTLSETVREAAFDALQSMG
HHHHHHHHHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQADTLAAQFDVAVIGSGPGGYEAAIHAARYGLKTCIVEKAVLGGVCVNWGCIPTKALL
CCCHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHH
RSAEVFDLAKNPETFGVNVGNVSFDLAQAVKRSRNVALKSSKGVAYLLKKAAVEVLAGEA
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCE
VLTGGAGVMVTMPDGSVRMLGAKNIIVATGSTPRVIPGLEPDGKKIITSREALILKEVPK
EEECCCEEEEECCCCCEEEEECCEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHCCC
SMIVVGGGAIGVEMAWFYAKAGSKVTIVELMPRMLPAEEAEVSEALKRSFEKAGITVHCG
EEEEECCCCHHHEEEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEC
AKLDNVAVSESGVSAELVVEGSAPQTLNASCLLVAVGVTGAIDGLGLDAVGVETERGFIR
CCCCCEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECHHCCCCCCCCEECCCCCCCEEE
TDGQCRTSAPGIYAIGDVRGGMLLAHKASAEAAIAVEAIAGKSPEPLSEPLIPRCVYAQP
CCCCCCCCCCCEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCEEEECCC
SVASVGLTEEAAVNAGYQVAVGRSQFAASGKANAYGQLEGFVKLVFDAATGKMLGGHLIG
CCCCCCCCHHHHCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHEEEHHCCCCHHCCEEEC
HDAVELIGELGLACRYGVTAGGLVNTVHAHPTLSETVREAAFDALQSMG
HHHHHHHHHHCCEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901