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The map label for this gene is ogt [H]

Identifier: 21674112

GI number: 21674112

Start: 1207354

End: 1207851

Strand: Reverse

Name: ogt [H]

Synonym: CT1289

Alternate gene names: 21674112

Gene position: 1207851-1207354 (Counterclockwise)

Preceding gene: 21674113

Following gene: 21674111

Centisome position: 56.05

GC content: 60.04

Gene sequence:

>498_bases
ATGCAGCAAGAGTCCATACTGACCAATTACAGTTCGTTTTCCTGCCGGCGTATGCCTGTGGGGCTGATCGGAATCAGGGC
GCATGGAGGGCGGGTTACTGATCTATTATTCATGCACGTAGAGCCGTCAACGGCTCTTAGCGCTCCGGGCTCGCGGTTGA
TCGATGAGGCTTTTCAGCAGCTCGAAGCGTGGTTTTCGGGACGGTTGCGGGAGTTTGCATTGCCATTGGTCGAGCCTCGA
TCCGCATTCGAGCGGCGAGTTCGGGAGGCGATGCTTGCCATTCCGTATGGGCAGACGGCTTCGTATGGCGAACTGGCGGC
AGCGATTGGCTCGCCGGGTGCTGCGCGGGCGGTAGGCGCGGCGTGCGGACGCAATCCGCTGCCGGTTATCGTGCCATGTC
ACCGGGTACTTGGAGCGGGAGGCCGGATTGGTGGGTATCGAGGCGGTACGGAAATGAAACGGTGGCTGCTCGATTTCGAA
CGCAGGAATTCCGGGTAA

Upstream 100 bases:

>100_bases
CCGGCAGCTACATCCCGGTTTCGGGCGGGTTGTTGATGCTGTCAGAGGGATGGAGGAGGATGAAAAGAATTTATGCAATG
CCAATTAACGGACACCACAA

Downstream 100 bases:

>100_bases
GTAACATCTTGCCGGTGACTTCGCGGGCTGGAATCCTCTTCGTTTTTTTCACAAATGTTAAATCCTCTTTTGATTTTTTC
GTTATATAGGTTGGCTCTCA

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MQQESILTNYSSFSCRRMPVGLIGIRAHGGRVTDLLFMHVEPSTALSAPGSRLIDEAFQQLEAWFSGRLREFALPLVEPR
SAFERRVREAMLAIPYGQTASYGELAAAIGSPGAARAVGAACGRNPLPVIVPCHRVLGAGGRIGGYRGGTEMKRWLLDFE
RRNSG

Sequences:

>Translated_165_residues
MQQESILTNYSSFSCRRMPVGLIGIRAHGGRVTDLLFMHVEPSTALSAPGSRLIDEAFQQLEAWFSGRLREFALPLVEPR
SAFERRVREAMLAIPYGQTASYGELAAAIGSPGAARAVGAACGRNPLPVIVPCHRVLGAGGRIGGYRGGTEMKRWLLDFE
RRNSG
>Mature_165_residues
MQQESILTNYSSFSCRRMPVGLIGIRAHGGRVTDLLFMHVEPSTALSAPGSRLIDEAFQQLEAWFSGRLREFALPLVEPR
SAFERRVREAMLAIPYGQTASYGELAAAIGSPGAARAVGAACGRNPLPVIVPCHRVLGAGGRIGGYRGGTEMKRWLLDFE
RRNSG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=116, Percent_Identity=39.6551724137931, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=46.6019417475728, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI1788542, Length=119, Percent_Identity=40.3361344537815, Blast_Score=90, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=51.25, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=51.25, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=41.6666666666667, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17916; Mature: 17916

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQESILTNYSSFSCRRMPVGLIGIRAHGGRVTDLLFMHVEPSTALSAPGSRLIDEAFQQ
CCCCHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHH
LEAWFSGRLREFALPLVEPRSAFERRVREAMLAIPYGQTASYGELAAAIGSPGAARAVGA
HHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHH
ACGRNPLPVIVPCHRVLGAGGRIGGYRGGTEMKRWLLDFERRNSG
HHCCCCCCEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQQESILTNYSSFSCRRMPVGLIGIRAHGGRVTDLLFMHVEPSTALSAPGSRLIDEAFQQ
CCCCHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHH
LEAWFSGRLREFALPLVEPRSAFERRVREAMLAIPYGQTASYGELAAAIGSPGAARAVGA
HHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHH
ACGRNPLPVIVPCHRVLGAGGRIGGYRGGTEMKRWLLDFERRNSG
HHCCCCCCEEEECHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]