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The map label for this gene is mexA [H]

Identifier: 21674111

GI number: 21674111

Start: 1206022

End: 1207212

Strand: Reverse

Name: mexA [H]

Synonym: CT1288

Alternate gene names: 21674111

Gene position: 1207212-1206022 (Counterclockwise)

Preceding gene: 21674112

Following gene: 21674110

Centisome position: 56.02

GC content: 62.05

Gene sequence:

>1191_bases
ATGAACACGATTCCCGCTCTTCGCAACCGCTTCTCCCTGCTGGCGCTTGGCGCGCTTCTCGGTTCATTTCTGCTCCTGCC
GGGTTGCGGCAAGAAGCAGGACGGCCCGAAGCAGATGCCTCCTGCCCTGCCTGCGATGAAAGTCGAGCTTTCTTCCGCCT
CTGTCACCACCGACTACTCCGTCAGGCTCGAAGGTCTGGCCGACGCGGAGATCAGGCCGCAGGTGGATGGAATGTTGCAA
GCGATTCTGGTCAAAGAGGGCGATTTGGTCAAAAAGGGGCAGCCGCTTTTCAAGATCGACGATTCGGTCTATCGTGAGCA
GTACAACACGGCGCTGGCCGCGCAACGCGCCGCCGAGGCGCAGGCGGCAGTGGCAAAGGTGAACGCCGAGAAGCTGGTGC
CGCTGGTCGAGAACAAGGTGGTTGCACCGGTGCAGCTCACCACAGCCAAAGCACAGGAGCAGGCGGCAAGAGCGCTGGCG
GCGCAGGCGGCGGCTTCGGCACGGTCGGCGGCGATCAATCTCGACTATACGGTGATCAAGGCCCCGGTGGCCGGCTATAT
CGGTCGCATTCCCTACCGGCAGGGCAATCTGCTCACCAAAAATCAGGCCGCCGCGCTGACGACACTTTCGGACGTGAACC
GCATGTATGCGGTCTTCAGCATCAGTGAGGCCGGTTTCGCCGACTTCAAGAAACTCTATCCCGGCGCGACGCTCCAGCAG
AAAATCGACGCCGTGCCACCGGTCAAACTGACACTCTCCGATGGAACCGTCTATCAGCATGACGGCAAGCTCGAATCGAT
CAGCGGCGACTTCGACGCCTCGACCGGCTCGATCCGCTTGCGCGTGTCGTTTCCCAATCCGGAAGGCTTTCTGCGTAGTG
GTAACTCCGGCACGGTGAGCCTTAGCTCCAACTACAATCATGTTATCCTCGTGCCGCAGTCGGCCACGGTCGAGTTGCAG
GACAAGGTGATGGTGACGCTGCTGAAGCCGGGCAACAAGGTGATGAAGCAGGTGATTTCGGTCGCGGGGCGGAGCGGCCC
GAACTACATCGTCAGCGAGGGGCTTAAGCCGGGCGACGTGATCGTGACCGCGGGTATCGAGCGGTTGCAGGACGGCATGG
TCATCAAGCCGCTCTCCGCAGCTGCGCCGGGCGCACCCGCACCGGCGGCGCAGACTCCGAACGCACGCTAA

Upstream 100 bases:

>100_bases
GTTTTTTTCACAAATGTTAAATCCTCTTTTGATTTTTTCGTTATATAGGTTGGCTCTCAACCTTTCTTTTATTCGGTTTT
TTTAGTCATACTGATACTCC

Downstream 100 bases:

>100_bases
ACCGCAGGGCATATGTTTGAACGTTTCATCTCCCGCCCTGTGCTGGCGACCGTCATTTCGGTCATCCTCGTGATTCTCGG
TCTGGTCAGCATGAGGCAGC

Product: multidrug resistance protein AcrA/AcrE family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MNTIPALRNRFSLLALGALLGSFLLLPGCGKKQDGPKQMPPALPAMKVELSSASVTTDYSVRLEGLADAEIRPQVDGMLQ
AILVKEGDLVKKGQPLFKIDDSVYREQYNTALAAQRAAEAQAAVAKVNAEKLVPLVENKVVAPVQLTTAKAQEQAARALA
AQAAASARSAAINLDYTVIKAPVAGYIGRIPYRQGNLLTKNQAAALTTLSDVNRMYAVFSISEAGFADFKKLYPGATLQQ
KIDAVPPVKLTLSDGTVYQHDGKLESISGDFDASTGSIRLRVSFPNPEGFLRSGNSGTVSLSSNYNHVILVPQSATVELQ
DKVMVTLLKPGNKVMKQVISVAGRSGPNYIVSEGLKPGDVIVTAGIERLQDGMVIKPLSAAAPGAPAPAAQTPNAR

Sequences:

>Translated_396_residues
MNTIPALRNRFSLLALGALLGSFLLLPGCGKKQDGPKQMPPALPAMKVELSSASVTTDYSVRLEGLADAEIRPQVDGMLQ
AILVKEGDLVKKGQPLFKIDDSVYREQYNTALAAQRAAEAQAAVAKVNAEKLVPLVENKVVAPVQLTTAKAQEQAARALA
AQAAASARSAAINLDYTVIKAPVAGYIGRIPYRQGNLLTKNQAAALTTLSDVNRMYAVFSISEAGFADFKKLYPGATLQQ
KIDAVPPVKLTLSDGTVYQHDGKLESISGDFDASTGSIRLRVSFPNPEGFLRSGNSGTVSLSSNYNHVILVPQSATVELQ
DKVMVTLLKPGNKVMKQVISVAGRSGPNYIVSEGLKPGDVIVTAGIERLQDGMVIKPLSAAAPGAPAPAAQTPNAR
>Mature_396_residues
MNTIPALRNRFSLLALGALLGSFLLLPGCGKKQDGPKQMPPALPAMKVELSSASVTTDYSVRLEGLADAEIRPQVDGMLQ
AILVKEGDLVKKGQPLFKIDDSVYREQYNTALAAQRAAEAQAAVAKVNAEKLVPLVENKVVAPVQLTTAKAQEQAARALA
AQAAASARSAAINLDYTVIKAPVAGYIGRIPYRQGNLLTKNQAAALTTLSDVNRMYAVFSISEAGFADFKKLYPGATLQQ
KIDAVPPVKLTLSDGTVYQHDGKLESISGDFDASTGSIRLRVSFPNPEGFLRSGNSGTVSLSSNYNHVILVPQSATVELQ
DKVMVTLLKPGNKVMKQVISVAGRSGPNYIVSEGLKPGDVIVTAGIERLQDGMVIKPLSAAAPGAPAPAAQTPNAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=374, Percent_Identity=31.8181818181818, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1789929, Length=376, Percent_Identity=28.1914893617021, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI1789665, Length=370, Percent_Identity=29.7297297297297, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI87082036, Length=332, Percent_Identity=25.3012048192771, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081796, Length=321, Percent_Identity=28.6604361370716, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41785; Mature: 41785

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIPALRNRFSLLALGALLGSFLLLPGCGKKQDGPKQMPPALPAMKVELSSASVTTDYS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECEE
VRLEGLADAEIRPQVDGMLQAILVKEGDLVKKGQPLFKIDDSVYREQYNTALAAQRAAEA
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHH
QAAVAKVNAEKLVPLVENKVVAPVQLTTAKAQEQAARALAAQAAASARSAAINLDYTVIK
HHHHHHCCHHHHHHHHCCCEECEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEE
APVAGYIGRIPYRQGNLLTKNQAAALTTLSDVNRMYAVFSISEAGFADFKKLYPGATLQQ
CCHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCCCCHHHH
KIDAVPPVKLTLSDGTVYQHDGKLESISGDFDASTGSIRLRVSFPNPEGFLRSGNSGTVS
HHCCCCCEEEEECCCEEEECCCCEEECCCCCCCCCCEEEEEEECCCCCHHEECCCCCEEE
LSSNYNHVILVPQSATVELQDKVMVTLLKPGNKVMKQVISVAGRSGPNYIVSEGLKPGDV
EECCCCEEEEECCCCCEEECCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCE
IVTAGIERLQDGMVIKPLSAAAPGAPAPAAQTPNAR
EEECCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTIPALRNRFSLLALGALLGSFLLLPGCGKKQDGPKQMPPALPAMKVELSSASVTTDYS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEECEE
VRLEGLADAEIRPQVDGMLQAILVKEGDLVKKGQPLFKIDDSVYREQYNTALAAQRAAEA
EEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHH
QAAVAKVNAEKLVPLVENKVVAPVQLTTAKAQEQAARALAAQAAASARSAAINLDYTVIK
HHHHHHCCHHHHHHHHCCCEECEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEE
APVAGYIGRIPYRQGNLLTKNQAAALTTLSDVNRMYAVFSISEAGFADFKKLYPGATLQQ
CCHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHHCCCCHHHH
KIDAVPPVKLTLSDGTVYQHDGKLESISGDFDASTGSIRLRVSFPNPEGFLRSGNSGTVS
HHCCCCCEEEEECCCEEEECCCCEEECCCCCCCCCCEEEEEEECCCCCHHEECCCCCEEE
LSSNYNHVILVPQSATVELQDKVMVTLLKPGNKVMKQVISVAGRSGPNYIVSEGLKPGDV
EECCCCEEEEECCCCCEEECCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCE
IVTAGIERLQDGMVIKPLSAAAPGAPAPAAQTPNAR
EEECCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]