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The map label for this gene is rlmB [H]

Identifier: 21674078

GI number: 21674078

Start: 1179613

End: 1180122

Strand: Reverse

Name: rlmB [H]

Synonym: CT1255

Alternate gene names: 21674078

Gene position: 1180122-1179613 (Counterclockwise)

Preceding gene: 21674079

Following gene: 21674077

Centisome position: 54.76

GC content: 60.2

Gene sequence:

>510_bases
ATGGGGCGGCTGACGCCCGAGGCCTATGCTGATTCAGCGCGGCATCCGGTGACGCTCATGCTGTACAATATCCGCAGCAT
GTGGAACGTCGGCGCCATGTTCCGGACAGCCGACGCGGCGGGCATCGATGAAATTGTCATCACTGGTTACACGGCCACGC
CGCCCCGCAAGGAGATCGACAAGACCGCGCTTGGCGCGCAGGAGACAGTGCCCTGGCGACACTTTGCCGATCCGGTCGAG
GCCATCACGGTGCTGAAAAGGGAGAGCAAAAAGATTTTCGGACTCGAAATCGCCGAGAACAGCCGAAGCTACAGTTCGCT
GACGGCGGGCGATTTTCCGCTGGCGCTGATCGTTGGCAACGAGGTTGAGGGGATCGGCGATGCGCTGCTCAGCCACTGTG
ATGGCGTGATCGAGATTCCGCAATATGGCGTGAAGCATTCACTGAACGTGGCGGTGGCTGCCGGAGTGGCGCTTTTCGAG
TGCGTTCGGGTGTTCAGGAAGAATGGTTGA

Upstream 100 bases:

>100_bases
CTCCGCTTCAATGTTGGTCTCAGGGAGGAGAACGACCGCCTGTTGGAACTGCTGAAAAAGCTCTGAGATGTCGGGTTTCA
GGCAGTTGACGGGAGACGAG

Downstream 100 bases:

>100_bases
ACTTTTATGGCCGTTACGGGCTTCTTCTTTTTATTTGTGGTATATTCAGGACGGATTTTAGTATTTCTGATCTTTTTCCC
GGTTGTTCCCTCCATTACTG

Product: SpoU rRNA methylase family protein

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MGRLTPEAYADSARHPVTLMLYNIRSMWNVGAMFRTADAAGIDEIVITGYTATPPRKEIDKTALGAQETVPWRHFADPVE
AITVLKRESKKIFGLEIAENSRSYSSLTAGDFPLALIVGNEVEGIGDALLSHCDGVIEIPQYGVKHSLNVAVAAGVALFE
CVRVFRKNG

Sequences:

>Translated_169_residues
MGRLTPEAYADSARHPVTLMLYNIRSMWNVGAMFRTADAAGIDEIVITGYTATPPRKEIDKTALGAQETVPWRHFADPVE
AITVLKRESKKIFGLEIAENSRSYSSLTAGDFPLALIVGNEVEGIGDALLSHCDGVIEIPQYGVKHSLNVAVAAGVALFE
CVRVFRKNG
>Mature_168_residues
GRLTPEAYADSARHPVTLMLYNIRSMWNVGAMFRTADAAGIDEIVITGYTATPPRKEIDKTALGAQETVPWRHFADPVEA
ITVLKRESKKIFGLEIAENSRSYSSLTAGDFPLALIVGNEVEGIGDALLSHCDGVIEIPQYGVKHSLNVAVAAGVALFEC
VRVFRKNG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=152, Percent_Identity=34.8684210526316, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 18347; Mature: 18216

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRLTPEAYADSARHPVTLMLYNIRSMWNVGAMFRTADAAGIDEIVITGYTATPPRKEID
CCCCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHH
KTALGAQETVPWRHFADPVEAITVLKRESKKIFGLEIAENSRSYSSLTAGDFPLALIVGN
HHHCCCCCCCCHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECC
EVEGIGDALLSHCDGVIEIPQYGVKHSLNVAVAAGVALFECVRVFRKNG
CCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GRLTPEAYADSARHPVTLMLYNIRSMWNVGAMFRTADAAGIDEIVITGYTATPPRKEID
CCCCCHHHHCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHH
KTALGAQETVPWRHFADPVEAITVLKRESKKIFGLEIAENSRSYSSLTAGDFPLALIVGN
HHHCCCCCCCCHHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEEECC
EVEGIGDALLSHCDGVIEIPQYGVKHSLNVAVAAGVALFECVRVFRKNG
CCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA