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The map label for this gene is ispB [H]

Identifier: 21674031

GI number: 21674031

Start: 1132450

End: 1133424

Strand: Reverse

Name: ispB [H]

Synonym: CT1206

Alternate gene names: 21674031

Gene position: 1133424-1132450 (Counterclockwise)

Preceding gene: 21674034

Following gene: 21674030

Centisome position: 52.6

GC content: 55.38

Gene sequence:

>975_bases
GTGGATATTAATGTGGTAACATTTTCTGTTACGGAAGAGCTGAAACAGTTCCAGGAGCGCTACAAAACGGTGCTTCATTC
AAGCAACAGCCTGGTTGACAAGGTCACGCGCTATGTGCTCCGGCAGCAGGGCAAGCAGATCAGGCCGACGCTGGTGATTC
TTGCGGCCAAGGTGTGCGGCGGCGTCCACGACGTCACCTATCGCGGCGCGATCATGGTGGAGCTGCTGCATTCGGCCACG
CTGATCCACGACGATGTGGTGGACGGTGCCGAAATGCGCCGGGGGATTCCCTCGATCAACGCTCTGTGGAAAAACAAGAT
ATCGGTGCTGATCGGTGACTATCTACTCTCCAAGGGTCTGCTCTACTCCCTCGAAAACAAGGATTACCGGTCGCTGCATT
TGGTCTCGGAGGCGGTTCGCCGCATGAGCGAGGGGGAGATTCTCCAGATCCAGAAAACTCGCAGCCTTGACATCACCGAG
GAGGATTATCTGAGTGTCATTGCCGACAAAACAGGCTCGCTCATCGCCACCTCGTGCGCCATCGGCGCGGCCAGCTCCAC
CGACTCCGAAGACGAGATCGCCAGCCTGAAGAGCTATGGCGAATTTCTCGGCCTGGCCTTCCAGATCAGGGATGACCTGC
TGGACTACACCGGGGATTCCAAAAAAACCGGCAAGCAGCTCGGCATCGATATCAAGGATCGCAAGATTACTCTTCCGCTG
ATCTACGCTCTTCGTCAATCGGACAAATCGGAGCAGAACAAGATCAAGTCGATTCTGAAAAGCTCCCGGAAGCGTTCGGT
CAGGAGCGGCGAAGTAATTGACTTTGTCACCCGGAAGGGGGGGCTTGATTATGCCGCCGAAGTTGCGGAAGGCTTTGCGG
ACAAGGCGCTCGAATCGATTGCCCATTTTCCCGAAAGTGACGCCAAGCGTTCACTCCAGCTTCTTGTCGATTTTGTCATG
AAACGGCAGCATTGA

Upstream 100 bases:

>100_bases
TTTGCTCAATCATGGAAATATCGATAACTTCTCACGGTTTTGAACGTAAGCGCAATCGCGCCGGGCATTTGACGGTGCAC
CAAGCACAAAGGATCAGGTT

Downstream 100 bases:

>100_bases
ACTCAACCAACGCCAATCCAGTATTTTCAGACCGGTCAGCCAAGATGAAAAAAGTACTGATATTTCTTCTGTTCCTTCTG
ATTATCGCCATTGTCGCCGT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MDINVVTFSVTEELKQFQERYKTVLHSSNSLVDKVTRYVLRQQGKQIRPTLVILAAKVCGGVHDVTYRGAIMVELLHSAT
LIHDDVVDGAEMRRGIPSINALWKNKISVLIGDYLLSKGLLYSLENKDYRSLHLVSEAVRRMSEGEILQIQKTRSLDITE
EDYLSVIADKTGSLIATSCAIGAASSTDSEDEIASLKSYGEFLGLAFQIRDDLLDYTGDSKKTGKQLGIDIKDRKITLPL
IYALRQSDKSEQNKIKSILKSSRKRSVRSGEVIDFVTRKGGLDYAAEVAEGFADKALESIAHFPESDAKRSLQLLVDFVM
KRQH

Sequences:

>Translated_324_residues
MDINVVTFSVTEELKQFQERYKTVLHSSNSLVDKVTRYVLRQQGKQIRPTLVILAAKVCGGVHDVTYRGAIMVELLHSAT
LIHDDVVDGAEMRRGIPSINALWKNKISVLIGDYLLSKGLLYSLENKDYRSLHLVSEAVRRMSEGEILQIQKTRSLDITE
EDYLSVIADKTGSLIATSCAIGAASSTDSEDEIASLKSYGEFLGLAFQIRDDLLDYTGDSKKTGKQLGIDIKDRKITLPL
IYALRQSDKSEQNKIKSILKSSRKRSVRSGEVIDFVTRKGGLDYAAEVAEGFADKALESIAHFPESDAKRSLQLLVDFVM
KRQH
>Mature_324_residues
MDINVVTFSVTEELKQFQERYKTVLHSSNSLVDKVTRYVLRQQGKQIRPTLVILAAKVCGGVHDVTYRGAIMVELLHSAT
LIHDDVVDGAEMRRGIPSINALWKNKISVLIGDYLLSKGLLYSLENKDYRSLHLVSEAVRRMSEGEILQIQKTRSLDITE
EDYLSVIADKTGSLIATSCAIGAASSTDSEDEIASLKSYGEFLGLAFQIRDDLLDYTGDSKKTGKQLGIDIKDRKITLPL
IYALRQSDKSEQNKIKSILKSSRKRSVRSGEVIDFVTRKGGLDYAAEVAEGFADKALESIAHFPESDAKRSLQLLVDFVM
KRQH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=29.5031055900621, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4758430, Length=267, Percent_Identity=24.3445692883895, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI83700220, Length=267, Percent_Identity=24.3445692883895, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1789578, Length=300, Percent_Identity=34.3333333333333, Blast_Score=188, Evalue=3e-49,
Organism=Escherichia coli, GI1786623, Length=287, Percent_Identity=28.2229965156794, Blast_Score=87, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=324, Percent_Identity=29.0123456790123, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6319475, Length=311, Percent_Identity=30.8681672025724, Blast_Score=124, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=254, Percent_Identity=25.9842519685039, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24651612, Length=328, Percent_Identity=29.2682926829268, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI281365769, Length=272, Percent_Identity=22.4264705882353, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24660002, Length=272, Percent_Identity=22.4264705882353, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 36158; Mature: 36158

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDINVVTFSVTEELKQFQERYKTVLHSSNSLVDKVTRYVLRQQGKQIRPTLVILAAKVCG
CCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GVHDVTYRGAIMVELLHSATLIHDDVVDGAEMRRGIPSINALWKNKISVLIGDYLLSKGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
LYSLENKDYRSLHLVSEAVRRMSEGEILQIQKTRSLDITEEDYLSVIADKTGSLIATSCA
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEHHHH
IGAASSTDSEDEIASLKSYGEFLGLAFQIRDDLLDYTGDSKKTGKQLGIDIKDRKITLPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEECCCEEHHHH
IYALRQSDKSEQNKIKSILKSSRKRSVRSGEVIDFVTRKGGLDYAAEVAEGFADKALESI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AHFPESDAKRSLQLLVDFVMKRQH
HHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDINVVTFSVTEELKQFQERYKTVLHSSNSLVDKVTRYVLRQQGKQIRPTLVILAAKVCG
CCCEEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
GVHDVTYRGAIMVELLHSATLIHDDVVDGAEMRRGIPSINALWKNKISVLIGDYLLSKGL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
LYSLENKDYRSLHLVSEAVRRMSEGEILQIQKTRSLDITEEDYLSVIADKTGSLIATSCA
EEEECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCEEEHHHH
IGAASSTDSEDEIASLKSYGEFLGLAFQIRDDLLDYTGDSKKTGKQLGIDIKDRKITLPL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCEECCCEEHHHH
IYALRQSDKSEQNKIKSILKSSRKRSVRSGEVIDFVTRKGGLDYAAEVAEGFADKALESI
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AHFPESDAKRSLQLLVDFVMKRQH
HHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]