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The map label for this gene is miaA

Identifier: 21673801

GI number: 21673801

Start: 918956

End: 919945

Strand: Reverse

Name: miaA

Synonym: CT0973

Alternate gene names: 21673801

Gene position: 919945-918956 (Counterclockwise)

Preceding gene: 21673802

Following gene: 21673800

Centisome position: 42.69

GC content: 56.87

Gene sequence:

>990_bases
ATGAATACTAAACCGGTTCTCGTGATCCTCGGCCCGACCGCCTCTGGCAAAACGGAGCTGGCCTTCCGCATCGCCCGCCA
GACCGGCGGCGAAATCATCTCGGCGGACTCGCGCCAGATCTATCGCGGCATGGACATCGGCACCGCCAAACCGCCGCGCT
GGATGCTCGACGAGGTCAAACACCACTTCATCGACAAAAAAGAGATCGGCGAACCGTTCAGCGCAGGAGATTTTGCGGAG
CAGGCTGCGGAAAAAATCCGGGAACTCCATCAACGCGGCATCACGCCAGTCGTTGCGGGCGGTTCGACGCTCTACCTCGA
AGGCCTGCTCAAAGGCTTCGCCGAACTGCCACCAGCCGACCCGGAAATCCGGGCGCAGCTGACCCGCGAACTCGAACGCC
ACGGCGCGGAAGCGCTCTACCGCCGACTCGAAGCGCTTGATCCCGAACAGGCTAAAACGCTCGACCCAACCAAAACCCAG
CGCCTTATCCGAAGCCTCGAAATCATCGAAATTTCGGGAACGACAGTCACGGCCCTGCAAAGCAAAACTCCCGGCCCGCC
AACTGGAATCAACTTCACTGTCATCGGTCTCGACCTGCCCCGCGAACTGCTCTATGAGCGCATCAACCAGCGCACAAGCG
CAATGATACAAGCCGGACTTGAAGCCGAAGCCCGTTATCTTTTCGACAAGTTTCGTGACGAATGGCGCAGCAAAAACCTC
AACGCGCTCGCCACCGTCGGCTACCGCGAACTCTTCGAACACTTCGAGGAATTGCACGATCTCGACACCGCGGTTTCACT
CATCGCCCAACACACCAGAAACTACGCCAAACGTCAGCTGACATTTTTCCGCAACAGGCTGGATGTCGAGTGGGTGAAAG
CACCACTTGACGAGGCGGAAATCGAAGCACTTGTAGAGTTTTTTTCCACCAGACAAGACGATAGCGTTCCGCATTCTCCT
ATTGCTATTGCAAAAAAACAAAACGCTTAA

Upstream 100 bases:

>100_bases
TCGTCACAGGCATCGACGGCTGCTACTACAACGTGGTCGGCCTGCCAGTCTCGAAGGTCTATGCTGCCCTCAAACCCCTT
TTTCCCGCCGAAGGGTAACA

Downstream 100 bases:

>100_bases
CCACTCCCCATCAGCGAGCAACCCACCCTCACCCACCTCAAACATTTTACGCCGTTGAAAAAAATTGATTTGAATGAATC
GAATTTTGTACGATAATAGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNTKPVLVILGPTASGKTELAFRIARQTGGEIISADSRQIYRGMDIGTAKPPRWMLDEVKHHFIDKKEIGEPFSAGDFAE
QAAEKIRELHQRGITPVVAGGSTLYLEGLLKGFAELPPADPEIRAQLTRELERHGAEALYRRLEALDPEQAKTLDPTKTQ
RLIRSLEIIEISGTTVTALQSKTPGPPTGINFTVIGLDLPRELLYERINQRTSAMIQAGLEAEARYLFDKFRDEWRSKNL
NALATVGYRELFEHFEELHDLDTAVSLIAQHTRNYAKRQLTFFRNRLDVEWVKAPLDEAEIEALVEFFSTRQDDSVPHSP
IAIAKKQNA

Sequences:

>Translated_329_residues
MNTKPVLVILGPTASGKTELAFRIARQTGGEIISADSRQIYRGMDIGTAKPPRWMLDEVKHHFIDKKEIGEPFSAGDFAE
QAAEKIRELHQRGITPVVAGGSTLYLEGLLKGFAELPPADPEIRAQLTRELERHGAEALYRRLEALDPEQAKTLDPTKTQ
RLIRSLEIIEISGTTVTALQSKTPGPPTGINFTVIGLDLPRELLYERINQRTSAMIQAGLEAEARYLFDKFRDEWRSKNL
NALATVGYRELFEHFEELHDLDTAVSLIAQHTRNYAKRQLTFFRNRLDVEWVKAPLDEAEIEALVEFFSTRQDDSVPHSP
IAIAKKQNA
>Mature_329_residues
MNTKPVLVILGPTASGKTELAFRIARQTGGEIISADSRQIYRGMDIGTAKPPRWMLDEVKHHFIDKKEIGEPFSAGDFAE
QAAEKIRELHQRGITPVVAGGSTLYLEGLLKGFAELPPADPEIRAQLTRELERHGAEALYRRLEALDPEQAKTLDPTKTQ
RLIRSLEIIEISGTTVTALQSKTPGPPTGINFTVIGLDLPRELLYERINQRTSAMIQAGLEAEARYLFDKFRDEWRSKNL
NALATVGYRELFEHFEELHDLDTAVSLIAQHTRNYAKRQLTFFRNRLDVEWVKAPLDEAEIEALVEFFSTRQDDSVPHSP
IAIAKKQNA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=29.1666666666667, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=37.7162629757785, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=271, Percent_Identity=26.9372693726937, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=25.9259259259259, Blast_Score=109, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24649830, Length=278, Percent_Identity=28.4172661870504, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_CHLTE (Q8KDS2)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661866.1
- ProteinModelPortal:   Q8KDS2
- SMR:   Q8KDS2
- GeneID:   1007058
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0973
- NMPDR:   fig|194439.1.peg.960
- TIGR:   CT0973
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK637545
- BioCyc:   CTEP194439:CT_0973-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 37068; Mature: 37068

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKPVLVILGPTASGKTELAFRIARQTGGEIISADSRQIYRGMDIGTAKPPRWMLDEVK
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHH
HHFIDKKEIGEPFSAGDFAEQAAEKIRELHQRGITPVVAGGSTLYLEGLLKGFAELPPAD
HHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCC
PEIRAQLTRELERHGAEALYRRLEALDPEQAKTLDPTKTQRLIRSLEIIEISGTTVTALQ
HHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEEECCCEEEEEC
SKTPGPPTGINFTVIGLDLPRELLYERINQRTSAMIQAGLEAEARYLFDKFRDEWRSKNL
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
NALATVGYRELFEHFEELHDLDTAVSLIAQHTRNYAKRQLTFFRNRLDVEWVKAPLDEAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHH
IEALVEFFSTRQDDSVPHSPIAIAKKQNA
HHHHHHHHHCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MNTKPVLVILGPTASGKTELAFRIARQTGGEIISADSRQIYRGMDIGTAKPPRWMLDEVK
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEECCCHHHHHCCCCCCCCCCHHHHHHHH
HHFIDKKEIGEPFSAGDFAEQAAEKIRELHQRGITPVVAGGSTLYLEGLLKGFAELPPAD
HHHCCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHCCCCC
PEIRAQLTRELERHGAEALYRRLEALDPEQAKTLDPTKTQRLIRSLEIIEISGTTVTALQ
HHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHEEEECCCEEEEEC
SKTPGPPTGINFTVIGLDLPRELLYERINQRTSAMIQAGLEAEARYLFDKFRDEWRSKNL
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
NALATVGYRELFEHFEELHDLDTAVSLIAQHTRNYAKRQLTFFRNRLDVEWVKAPLDEAE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHH
IEALVEFFSTRQDDSVPHSPIAIAKKQNA
HHHHHHHHHCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901