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The map label for this gene is btuB [H]

Identifier: 21673763

GI number: 21673763

Start: 882927

End: 884954

Strand: Direct

Name: btuB [H]

Synonym: CT0935

Alternate gene names: 21673763

Gene position: 882927-884954 (Clockwise)

Preceding gene: 21673762

Following gene: 21673764

Centisome position: 40.97

GC content: 55.72

Gene sequence:

>2028_bases
GTGTTCTGGTGCCGGTTCAGTCCTTTTCCTCTATCCCTGTCTGTTGATGATTCAGTTAATTCAACACAGGACAACACCAT
GAACAGAAGAACGCTCGCCATCGTCATGGCGGCAATGCTCGCCAGCCCCACGCTCCACGCCGCCGAGACCGGTTCGGACT
ATCTCGGTAATGAAATCGTCGTCACGTCGAGCCGTGTGCCGCAGCCGAAAAAAGAGCTGACCTCGAACGTTACGATCATC
ACCAAAGAGGAGATTAACGAGTCGTCGGCCAACGATCTCAGTGAACTTCTCGCGGAGAAAAATCTTGGACAGATTCAAAA
GTATCCTGGAACCTTGACCTCCGTTGGCATCCGCGGTTTCAGGACTGAATCCCACGGCAATGATCTCATGGGCAAGGTGC
TTGTACTGCTCGACGGCCGCCGCGCCGGTACCGGAAACCTCGCCAAGATCATGACAGCCAACGTCGAGCGCATCGAAATC
ATTAGGGGGCCTGCCGCCGTGCAGTACGGTTCTGCGGCGATTGGCGGCGTGATCAACGTGATTACGGCCAAAGGTTCGGG
CGCGCCGTCGGCGTCGATCGAGCAGAAAATCGGCAGCAACGATTTCTCCATGACCGAGGCGACCATTCAGGGAAAATCCG
GACGCTTCGACTATTCAGGCAGCATCTCGAAATCCGATTCTGGGAGCTACAAGACGGCCAAAGGCGAGACCTACCAGAAC
ACCGGCTACCACGACCAGAAGATGGCGAGCCTGAATGTTGGTTACGAGATTGCCGACGGCCAGCGGATCGGCATGATCGT
TCACTCCTTCGATGTGGACAAGGCCGGCTCTCCGGGCTATTTCAGCTTGCCCGATCTCAATGCCTATACGGTTCAGAACA
ACCACTCCGTCGATTTCAGGTACGATGGTACGACCTCCAGCCGTACTCTTTCATGGATGGCTCGCTACTTCACCGGAAAG
GACGAGTATCGCTATGTCGATCCGAACTGGGGTGAAAGCACCAACAATGTTGACCAGCAAGGGGCGCAGGCACAGGTTTC
CTGGCAGCCGGGTGCCCTCAGGCTTACAGCCGGATTTGACTGGTTGAAATACGAGCTCGATTCCACTTCGGCGCCCACAT
GGGCCTCTTATGAAAATCCGGCCGGTTTTCTTCTCGGCAAATATGGCCTGTTCGACGAGCGCCTGATTCTTACCGCAGGC
GTGCGTTACGATGATTATCATGTTGACATGAAGGAAGGCGAGGGCACGTCGAAGAGCAGGGACAACTTCGCGCATCAGGC
CGGAGTGGCGTGGCAGGCAACCGATTTCCTCAAGCTCAGAGGTTCGTATGCCGAAGGCTTCAGGATGCCGTCGGCGCGTG
AGCTTGCCGCCCATATCACTAGCTGGGGGACGACCTATATCGGCAATCCCGATCTCAAACCGGAATCGAGCGAGACGTAT
GAAGTCGGCATGGACCTTACCTGGAAAGGGCTCAATGGCTCGCTGACCTGGTTCAGCACCGACTACAAGGATATGATTCA
GACCAAAGCGATTGCCCCAACGACCTACACCTATATCAATGTTGGCAGCTCGACGGTATCCGGAATCGAAGCTGAGCTTT
CGAAGGTGTTTGCACTCGAGGGTTCTTCGTGGACGCTTGAACCATATGCCGGGTACACCTACCTCTTGCGGTATCGTGAC
AACCAGACCGGTGACGACCTGCTCTACACGCCGCAGTGGAACGCCAGCACCGGACTGAGAGTGCATGATGGGTGCGGGTT
CAACGGCGCGTTCAACCTTGCCTACACAGGCAAGACTCTCGTTCAGAACTGGGAGACCGGCTTTGGCGAGGTGGTGCCGA
AAGGCGGATTTACGGTGGTTGATCTTTCCGTATCGAAGAAGTTTCCGTTCGGAGGCAAGGGCGTGAAAGGGCCGGGAATC
ACGGTCAGGGCGGAGATTAACAACCTTTTTGACCGCGAGTACCAGTATGTCAAGGGCTATCCGATGCCTGGCCGCACCTT
TGTTTTTGGTCTGAAGGCGGACATTTGA

Upstream 100 bases:

>100_bases
CCGGTTTATCCAAAGACCAACAGCATCGTTTTCAGAATCCGTGCTCTCTGGCGGTATTTTCCGTTGAGCGCGGCTTTCGT
CAGTCCGTTCGATCCATGCT

Downstream 100 bases:

>100_bases
TCCATGCTGTCATCAACAGAAACCGGTAAATCATTGCCGGTCATGAAACAGTTGATGTTTGTGCTGCTGCTCCTGCTTTT
CGCGGGAGCGGCATCCGGGT

Product: TonB-dependent receptor-related protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MFWCRFSPFPLSLSVDDSVNSTQDNTMNRRTLAIVMAAMLASPTLHAAETGSDYLGNEIVVTSSRVPQPKKELTSNVTII
TKEEINESSANDLSELLAEKNLGQIQKYPGTLTSVGIRGFRTESHGNDLMGKVLVLLDGRRAGTGNLAKIMTANVERIEI
IRGPAAVQYGSAAIGGVINVITAKGSGAPSASIEQKIGSNDFSMTEATIQGKSGRFDYSGSISKSDSGSYKTAKGETYQN
TGYHDQKMASLNVGYEIADGQRIGMIVHSFDVDKAGSPGYFSLPDLNAYTVQNNHSVDFRYDGTTSSRTLSWMARYFTGK
DEYRYVDPNWGESTNNVDQQGAQAQVSWQPGALRLTAGFDWLKYELDSTSAPTWASYENPAGFLLGKYGLFDERLILTAG
VRYDDYHVDMKEGEGTSKSRDNFAHQAGVAWQATDFLKLRGSYAEGFRMPSARELAAHITSWGTTYIGNPDLKPESSETY
EVGMDLTWKGLNGSLTWFSTDYKDMIQTKAIAPTTYTYINVGSSTVSGIEAELSKVFALEGSSWTLEPYAGYTYLLRYRD
NQTGDDLLYTPQWNASTGLRVHDGCGFNGAFNLAYTGKTLVQNWETGFGEVVPKGGFTVVDLSVSKKFPFGGKGVKGPGI
TVRAEINNLFDREYQYVKGYPMPGRTFVFGLKADI

Sequences:

>Translated_675_residues
MFWCRFSPFPLSLSVDDSVNSTQDNTMNRRTLAIVMAAMLASPTLHAAETGSDYLGNEIVVTSSRVPQPKKELTSNVTII
TKEEINESSANDLSELLAEKNLGQIQKYPGTLTSVGIRGFRTESHGNDLMGKVLVLLDGRRAGTGNLAKIMTANVERIEI
IRGPAAVQYGSAAIGGVINVITAKGSGAPSASIEQKIGSNDFSMTEATIQGKSGRFDYSGSISKSDSGSYKTAKGETYQN
TGYHDQKMASLNVGYEIADGQRIGMIVHSFDVDKAGSPGYFSLPDLNAYTVQNNHSVDFRYDGTTSSRTLSWMARYFTGK
DEYRYVDPNWGESTNNVDQQGAQAQVSWQPGALRLTAGFDWLKYELDSTSAPTWASYENPAGFLLGKYGLFDERLILTAG
VRYDDYHVDMKEGEGTSKSRDNFAHQAGVAWQATDFLKLRGSYAEGFRMPSARELAAHITSWGTTYIGNPDLKPESSETY
EVGMDLTWKGLNGSLTWFSTDYKDMIQTKAIAPTTYTYINVGSSTVSGIEAELSKVFALEGSSWTLEPYAGYTYLLRYRD
NQTGDDLLYTPQWNASTGLRVHDGCGFNGAFNLAYTGKTLVQNWETGFGEVVPKGGFTVVDLSVSKKFPFGGKGVKGPGI
TVRAEINNLFDREYQYVKGYPMPGRTFVFGLKADI
>Mature_675_residues
MFWCRFSPFPLSLSVDDSVNSTQDNTMNRRTLAIVMAAMLASPTLHAAETGSDYLGNEIVVTSSRVPQPKKELTSNVTII
TKEEINESSANDLSELLAEKNLGQIQKYPGTLTSVGIRGFRTESHGNDLMGKVLVLLDGRRAGTGNLAKIMTANVERIEI
IRGPAAVQYGSAAIGGVINVITAKGSGAPSASIEQKIGSNDFSMTEATIQGKSGRFDYSGSISKSDSGSYKTAKGETYQN
TGYHDQKMASLNVGYEIADGQRIGMIVHSFDVDKAGSPGYFSLPDLNAYTVQNNHSVDFRYDGTTSSRTLSWMARYFTGK
DEYRYVDPNWGESTNNVDQQGAQAQVSWQPGALRLTAGFDWLKYELDSTSAPTWASYENPAGFLLGKYGLFDERLILTAG
VRYDDYHVDMKEGEGTSKSRDNFAHQAGVAWQATDFLKLRGSYAEGFRMPSARELAAHITSWGTTYIGNPDLKPESSETY
EVGMDLTWKGLNGSLTWFSTDYKDMIQTKAIAPTTYTYINVGSSTVSGIEAELSKVFALEGSSWTLEPYAGYTYLLRYRD
NQTGDDLLYTPQWNASTGLRVHDGCGFNGAFNLAYTGKTLVQNWETGFGEVVPKGGFTVVDLSVSKKFPFGGKGVKGPGI
TVRAEINNLFDREYQYVKGYPMPGRTFVFGLKADI

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=681, Percent_Identity=25.5506607929515, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788478, Length=575, Percent_Identity=26.7826086956522, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1787723, Length=585, Percent_Identity=23.9316239316239, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786798, Length=192, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 73910; Mature: 73910

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWCRFSPFPLSLSVDDSVNSTQDNTMNRRTLAIVMAAMLASPTLHAAETGSDYLGNEIV
CEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEE
VTSSRVPQPKKELTSNVTIITKEEINESSANDLSELLAEKNLGQIQKYPGTLTSVGIRGF
EECCCCCCCHHHHHCCEEEEEHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHHCCEEE
RTESHGNDLMGKVLVLLDGRRAGTGNLAKIMTANVERIEIIRGPAAVQYGSAAIGGVINV
EECCCCCHHCEEEEEEECCCCCCCCCEEEEEECCCEEEEEEECCCCEECCCHHHCCEEEE
ITAKGSGAPSASIEQKIGSNDFSMTEATIQGKSGRFDYSGSISKSDSGSYKTAKGETYQN
EEECCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCC
TGYHDQKMASLNVGYEIADGQRIGMIVHSFDVDKAGSPGYFSLPDLNAYTVQNNHSVDFR
CCCCCCEEEEEECCEEECCCCEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEE
YDGTTSSRTLSWMARYFTGKDEYRYVDPNWGESTNNVDQQGAQAQVSWQPGALRLTAGFD
ECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCEEEEEECCCEEEEEECCC
WLKYELDSTSAPTWASYENPAGFLLGKYGLFDERLILTAGVRYDDYHVDMKEGEGTSKSR
EEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCEECCEEEEECCCCCCCCCH
DNFAHQAGVAWQATDFLKLRGSYAEGFRMPSARELAAHITSWGTTYIGNPDLKPESSETY
HHHHHHCCCEEEECCEEEECCCHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCEE
EVGMDLTWKGLNGSLTWFSTDYKDMIQTKAIAPTTYTYINVGSSTVSGIEAELSKVFALE
EECCEEEEECCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHEEEEEEC
GSSWTLEPYAGYTYLLRYRDNQTGDDLLYTPQWNASTGLRVHDGCGFNGAFNLAYTGKTL
CCCEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCEEECCCCCCCEEEEEECCHHH
VQNWETGFGEVVPKGGFTVVDLSVSKKFPFGGKGVKGPGITVRAEINNLFDREYQYVKGY
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCC
PMPGRTFVFGLKADI
CCCCCEEEEEEEECC
>Mature Secondary Structure
MFWCRFSPFPLSLSVDDSVNSTQDNTMNRRTLAIVMAAMLASPTLHAAETGSDYLGNEIV
CEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECCCCCCCCCCEEE
VTSSRVPQPKKELTSNVTIITKEEINESSANDLSELLAEKNLGQIQKYPGTLTSVGIRGF
EECCCCCCCHHHHHCCEEEEEHHHCCCCCHHHHHHHHHHCCCCHHHHCCCCHHHHCCEEE
RTESHGNDLMGKVLVLLDGRRAGTGNLAKIMTANVERIEIIRGPAAVQYGSAAIGGVINV
EECCCCCHHCEEEEEEECCCCCCCCCEEEEEECCCEEEEEEECCCCEECCCHHHCCEEEE
ITAKGSGAPSASIEQKIGSNDFSMTEATIQGKSGRFDYSGSISKSDSGSYKTAKGETYQN
EEECCCCCCCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCC
TGYHDQKMASLNVGYEIADGQRIGMIVHSFDVDKAGSPGYFSLPDLNAYTVQNNHSVDFR
CCCCCCEEEEEECCEEECCCCEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEE
YDGTTSSRTLSWMARYFTGKDEYRYVDPNWGESTNNVDQQGAQAQVSWQPGALRLTAGFD
ECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHCCCEEEEEECCCEEEEEECCC
WLKYELDSTSAPTWASYENPAGFLLGKYGLFDERLILTAGVRYDDYHVDMKEGEGTSKSR
EEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCEEEEEECCEECCEEEEECCCCCCCCCH
DNFAHQAGVAWQATDFLKLRGSYAEGFRMPSARELAAHITSWGTTYIGNPDLKPESSETY
HHHHHHCCCEEEECCEEEECCCHHHHCCCCCHHHHHHHHHHCCCEECCCCCCCCCCCCEE
EVGMDLTWKGLNGSLTWFSTDYKDMIQTKAIAPTTYTYINVGSSTVSGIEAELSKVFALE
EECCEEEEECCCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHEEEEEEC
GSSWTLEPYAGYTYLLRYRDNQTGDDLLYTPQWNASTGLRVHDGCGFNGAFNLAYTGKTL
CCCEEECCCCCEEEEEEEECCCCCCCEEECCCCCCCCCCEEECCCCCCCEEEEEECCHHH
VQNWETGFGEVVPKGGFTVVDLSVSKKFPFGGKGVKGPGITVRAEINNLFDREYQYVKGY
HHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCC
PMPGRTFVFGLKADI
CCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA