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The map label for this gene is ribE [H]

Identifier: 21673586

GI number: 21673586

Start: 730917

End: 731576

Strand: Direct

Name: ribE [H]

Synonym: CT0756

Alternate gene names: 21673586

Gene position: 730917-731576 (Clockwise)

Preceding gene: 21673585

Following gene: 21673587

Centisome position: 33.92

GC content: 63.79

Gene sequence:

>660_bases
ATGTTCACTGGCATAGTTAAGGATGTTGGAGCCATCGCTGCTTCGGCGCGGCAGGGGAGCGGGATGCGGCTGAAGGTTCG
CTACACTTCTGAGGCGGAGTTCGGCGATCTCGCTATCGATGAGAGCGTGAGTATCAATGGAGCTTGCCAGACGGCGGTGG
CGGTCGGGCCGGGGTGGTTCGAGGTTGATACGGTGGCCGAGACGCTCAAGAAGACGACGCTTGGCTCGTTCCGTCCGGGT
ACGAAGGTCAATCTCGAACGGGCCGTGCGGCCGATGGATCGGCTTGGCGGGCACTTCGTGCTGGGCCACGTCGATGGCGT
CGGGCGGGTGTTGCGCATCGAGGAGGTTGGCGGCAGCCGGATGATTTCGGTGGCGTTTGACTCGCGCTTCGATGCGTGGA
TCGTCTCTGCCGGCTCCATCGCCATCGACGGCGTCAGCCTCACCGTGGCGTCGGTCGAGCCGGGCCAGTTTACGGTGGCG
ATCATTCCCTACACCTTTGGCCATACCACCATCACCGGTCTCGCCGCCGGCAGCGAGGTGAACCTTGAATTCGACATTCT
CGGCAAGTACGTCGCCCGGCAGCATACGGCGGCCGCCGCGCCGTCGCAGGAGCCGAGCCGCATTACCGAATCGTGGTTGA
GCGGCCAGGGGTTTGCATGA

Upstream 100 bases:

>100_bases
TGTTACTATTGTTGTCAGATAAATGGTATTGCGGCTGTCTTAAAACCTGGGAGCTCGGCGTTCCCAAGGGCATCACGAAA
GAAACGAATCAAACAAATAT

Downstream 100 bases:

>100_bases
TGCCGGACGAACACTCGCAATCCGATCTTTTCGGTTTCTCCGATCCGGGCCGCTCGTCCGCGAAAAGCAACCGCTTTCAG
CCGCTTGCCGAACGGGTGCG

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MFTGIVKDVGAIAASARQGSGMRLKVRYTSEAEFGDLAIDESVSINGACQTAVAVGPGWFEVDTVAETLKKTTLGSFRPG
TKVNLERAVRPMDRLGGHFVLGHVDGVGRVLRIEEVGGSRMISVAFDSRFDAWIVSAGSIAIDGVSLTVASVEPGQFTVA
IIPYTFGHTTITGLAAGSEVNLEFDILGKYVARQHTAAAAPSQEPSRITESWLSGQGFA

Sequences:

>Translated_219_residues
MFTGIVKDVGAIAASARQGSGMRLKVRYTSEAEFGDLAIDESVSINGACQTAVAVGPGWFEVDTVAETLKKTTLGSFRPG
TKVNLERAVRPMDRLGGHFVLGHVDGVGRVLRIEEVGGSRMISVAFDSRFDAWIVSAGSIAIDGVSLTVASVEPGQFTVA
IIPYTFGHTTITGLAAGSEVNLEFDILGKYVARQHTAAAAPSQEPSRITESWLSGQGFA
>Mature_219_residues
MFTGIVKDVGAIAASARQGSGMRLKVRYTSEAEFGDLAIDESVSINGACQTAVAVGPGWFEVDTVAETLKKTTLGSFRPG
TKVNLERAVRPMDRLGGHFVLGHVDGVGRVLRIEEVGGSRMISVAFDSRFDAWIVSAGSIAIDGVSLTVASVEPGQFTVA
IIPYTFGHTTITGLAAGSEVNLEFDILGKYVARQHTAAAAPSQEPSRITESWLSGQGFA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=186, Percent_Identity=34.9462365591398, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319733, Length=205, Percent_Identity=33.6585365853659, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23062; Mature: 23062

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVKDVGAIAASARQGSGMRLKVRYTSEAEFGDLAIDESVSINGACQTAVAVGPGWF
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCCEECCCCEEEEEEECCCCE
EVDTVAETLKKTTLGSFRPGTKVNLERAVRPMDRLGGHFVLGHVDGVGRVLRIEEVGGSR
EHHHHHHHHHHHHCCCCCCCCEECHHHHHCHHHHCCCEEEEEEECCCCEEEEEEECCCCE
MISVAFDSRFDAWIVSAGSIAIDGVSLTVASVEPGQFTVAIIPYTFGHTTITGLAAGSEV
EEEEEECCCCCEEEEECCCEEECCEEEEEEEECCCCEEEEEEEEECCCCEEEEEECCCEE
NLEFDILGKYVARQHTAAAAPSQEPSRITESWLSGQGFA
EEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFTGIVKDVGAIAASARQGSGMRLKVRYTSEAEFGDLAIDESVSINGACQTAVAVGPGWF
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEECCCEECCCCEEEEEEECCCCE
EVDTVAETLKKTTLGSFRPGTKVNLERAVRPMDRLGGHFVLGHVDGVGRVLRIEEVGGSR
EHHHHHHHHHHHHCCCCCCCCEECHHHHHCHHHHCCCEEEEEEECCCCEEEEEEECCCCE
MISVAFDSRFDAWIVSAGSIAIDGVSLTVASVEPGQFTVAIIPYTFGHTTITGLAAGSEV
EEEEEECCCCCEEEEECCCEEECCEEEEEEEECCCCEEEEEEEEECCCCEEEEEECCCEE
NLEFDILGKYVARQHTAAAAPSQEPSRITESWLSGQGFA
EEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2106516; 7934829; 9384377; 7473709 [H]