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The map label for this gene is kefB [H]

Identifier: 21673546

GI number: 21673546

Start: 697543

End: 699402

Strand: Direct

Name: kefB [H]

Synonym: CT0716

Alternate gene names: 21673546

Gene position: 697543-699402 (Clockwise)

Preceding gene: 21673545

Following gene: 21673547

Centisome position: 32.37

GC content: 57.15

Gene sequence:

>1860_bases
ATGATTCTTCTCTGTTTTCCCGGTTCTGAACAAGGTATTGGAATGCATCAGTTTGAATTTCTTGGACAACTGGTCCTGAT
CGGGGCGCTTGCCATTGTCAATATTCTCGTGTTCCAGAAGATCCGGATTCCTCCGGTGATCGGCCTGATCTTCACGGGTA
TCATGCTCGGGCCGACGGGGTTCCATGTTATCCGGAACAGCGGGCTGATTTCGACGCTTGCCGAAATGGGCGTCGTGCTG
CTGCTTTTTACCATCGGGCTGGAGTTTTCGGCCGATGATCTGAAAAAACTCCGGAAGATCGTCCTGTTCGGCGGAACGGC
GCAGATTCTCCTGACCGGTCTGGTGATCGCCATGTTTTCGTACTGGCTGATGGATGCAATCGGCAAGTCGGTTGGCAGCA
AGGAGGCGCTCGTGCTTGGCTTTTCGTTTTCGGTTAGCAGTACCGCGCTGTGCCTGAAAATTCTCTCTGACCGGGGCGAA
CTTGGTTTCGATCACGGCAAGATCGCCCTTGGCATCCTGATCTTTCAGGACATGGCCATTGTGCCGCTGATGTTCGGATT
CAGCTTTCTGACCCGCGGCAGCTCGATGCCGCTCGAATCATCGTTTGAGGAGATCGCGCTCTTGCTGCTCTTCGCCATCG
GCATGTTCGGCGGATTCCGGCTGCTGATGCCGAAAATCGTCCGGATCATCACCGAGCTGCACGCGGGCGAGGTGCTGGTG
CTGGGCGCACTGGTGCTCTGCTTTGGCGCGGCATGGCTCGCCTCGCTCATCGGCCTTTCCCTTGCGCTCGGCGCGTTTAT
GGCCGGAATGGTGATCGCCAGTACCGACGGCAGCCACCGCATCAGCCGCACCATCGATCCTTTCAGGGAGGCGATGACCA
GCATCTTTTTCGTCTCGGTCGGTCTGTTGCTCGACGTGAACATGATCGAGCTGCCGTGGCTCATCGCTATCGCCCTCGTG
GTGCTGGTGGTCAAAGGGTTGATCATGACCGGCATTTTGATGGCGCTTGGTTTTTCGTTGCGGGTCAGCCTCATGTCGGG
CATGGTGCTGGCACAGATTGGCGAGTTTTCCTTTGTGCTCGCCGGTACAGCCAAAGATGCCGGGCTGCTTGACCAGCACA
TGTTCCAGTCGATGCTGGCGGTCATTGTCGTTACCATGATCGTCACCCCGGCGCTGATCTCCGCCGCGCCGAAATTTGCT
GCCCAGGTCGCGCCAGCGCTCGGCTTTATGCCACTCGCATCCAAACCAGAGCCGAAACAGCCAGCGCGCGCCGCTGCCGG
GCCGATTGTCTGCCGGGGTGAAATTCACGCCGCCATTATCGGATTCGGCCTGATCGGCAGGAATATCGCCGCCGTGATGA
ATGCCACAAACCTGAACTATACGGTGCTTGATACCGACCGCAAGACTGTCAAGACGATGCGCCGCCAGGGCGAGCCGCTT
TTTTATGGTGACTGCACGGAGCGCAAGTCACTGTTGCGTATTGGCGTCGATCACTCCCGCGCCGTGGTGATCTGCATTCC
GGAAATCGACGCGGCCATTCAGTGCATCCGCCTCGTGCGGGAGATCAATCCCGGCGCGTTCATCATTGTGCGTTCCCGTT
CACTCGAATCGACGAACCGTTTTTACCGCGCCGGAGCCGATGCTGCCGTGACCGAGCTGTTCGAGACATCGATCCAGATG
TTTTCCGAACTGCTCAAACACTTCAGGGTCGAACCTGAAACGATTCTCGCTCAACAGGAAATTATCAGGCGCGAGGGCGG
CAACATTTTCCGTGAACTGGTAACTGAAACGGATGGTTCCGGCGACGATCCAGCTAAAAAATCTCAAAATGGCGCGGGCT
TCGTCACTTCTGAAACGTGA

Upstream 100 bases:

>100_bases
CAAAATATTTGAGGTGCATTTGCGCCTTTTCTCTTGGTATCTTACACTGAAGCAGACCTGACGCACCACCGCTTGTGCAT
GGCGCGCATACTGAGTTGCA

Downstream 100 bases:

>100_bases
TGGCAACATAGCGTGCAATCGTGATTTTTACATACATTGAACGGTAGTGTCCATCTGAACTTCTTTTGAAAAGTCATGGA
TAACGTGCCGGAACCATCGT

Product: glutathione-regulated potassium-efflux system protein KefC, putative

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MILLCFPGSEQGIGMHQFEFLGQLVLIGALAIVNILVFQKIRIPPVIGLIFTGIMLGPTGFHVIRNSGLISTLAEMGVVL
LLFTIGLEFSADDLKKLRKIVLFGGTAQILLTGLVIAMFSYWLMDAIGKSVGSKEALVLGFSFSVSSTALCLKILSDRGE
LGFDHGKIALGILIFQDMAIVPLMFGFSFLTRGSSMPLESSFEEIALLLLFAIGMFGGFRLLMPKIVRIITELHAGEVLV
LGALVLCFGAAWLASLIGLSLALGAFMAGMVIASTDGSHRISRTIDPFREAMTSIFFVSVGLLLDVNMIELPWLIAIALV
VLVVKGLIMTGILMALGFSLRVSLMSGMVLAQIGEFSFVLAGTAKDAGLLDQHMFQSMLAVIVVTMIVTPALISAAPKFA
AQVAPALGFMPLASKPEPKQPARAAAGPIVCRGEIHAAIIGFGLIGRNIAAVMNATNLNYTVLDTDRKTVKTMRRQGEPL
FYGDCTERKSLLRIGVDHSRAVVICIPEIDAAIQCIRLVREINPGAFIIVRSRSLESTNRFYRAGADAAVTELFETSIQM
FSELLKHFRVEPETILAQQEIIRREGGNIFRELVTETDGSGDDPAKKSQNGAGFVTSET

Sequences:

>Translated_619_residues
MILLCFPGSEQGIGMHQFEFLGQLVLIGALAIVNILVFQKIRIPPVIGLIFTGIMLGPTGFHVIRNSGLISTLAEMGVVL
LLFTIGLEFSADDLKKLRKIVLFGGTAQILLTGLVIAMFSYWLMDAIGKSVGSKEALVLGFSFSVSSTALCLKILSDRGE
LGFDHGKIALGILIFQDMAIVPLMFGFSFLTRGSSMPLESSFEEIALLLLFAIGMFGGFRLLMPKIVRIITELHAGEVLV
LGALVLCFGAAWLASLIGLSLALGAFMAGMVIASTDGSHRISRTIDPFREAMTSIFFVSVGLLLDVNMIELPWLIAIALV
VLVVKGLIMTGILMALGFSLRVSLMSGMVLAQIGEFSFVLAGTAKDAGLLDQHMFQSMLAVIVVTMIVTPALISAAPKFA
AQVAPALGFMPLASKPEPKQPARAAAGPIVCRGEIHAAIIGFGLIGRNIAAVMNATNLNYTVLDTDRKTVKTMRRQGEPL
FYGDCTERKSLLRIGVDHSRAVVICIPEIDAAIQCIRLVREINPGAFIIVRSRSLESTNRFYRAGADAAVTELFETSIQM
FSELLKHFRVEPETILAQQEIIRREGGNIFRELVTETDGSGDDPAKKSQNGAGFVTSET
>Mature_619_residues
MILLCFPGSEQGIGMHQFEFLGQLVLIGALAIVNILVFQKIRIPPVIGLIFTGIMLGPTGFHVIRNSGLISTLAEMGVVL
LLFTIGLEFSADDLKKLRKIVLFGGTAQILLTGLVIAMFSYWLMDAIGKSVGSKEALVLGFSFSVSSTALCLKILSDRGE
LGFDHGKIALGILIFQDMAIVPLMFGFSFLTRGSSMPLESSFEEIALLLLFAIGMFGGFRLLMPKIVRIITELHAGEVLV
LGALVLCFGAAWLASLIGLSLALGAFMAGMVIASTDGSHRISRTIDPFREAMTSIFFVSVGLLLDVNMIELPWLIAIALV
VLVVKGLIMTGILMALGFSLRVSLMSGMVLAQIGEFSFVLAGTAKDAGLLDQHMFQSMLAVIVVTMIVTPALISAAPKFA
AQVAPALGFMPLASKPEPKQPARAAAGPIVCRGEIHAAIIGFGLIGRNIAAVMNATNLNYTVLDTDRKTVKTMRRQGEPL
FYGDCTERKSLLRIGVDHSRAVVICIPEIDAAIQCIRLVREINPGAFIIVRSRSLESTNRFYRAGADAAVTELFETSIQM
FSELLKHFRVEPETILAQQEIIRREGGNIFRELVTETDGSGDDPAKKSQNGAGFVTSET

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=412, Percent_Identity=28.8834951456311, Blast_Score=116, Evalue=8e-26,
Organism=Escherichia coli, GI1789749, Length=579, Percent_Identity=27.2884283246978, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786232, Length=590, Percent_Identity=25.9322033898305, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1786685, Length=565, Percent_Identity=28.4955752212389, Blast_Score=175, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66660; Mature: 66660

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILLCFPGSEQGIGMHQFEFLGQLVLIGALAIVNILVFQKIRIPPVIGLIFTGIMLGPTG
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
FHVIRNSGLISTLAEMGVVLLLFTIGLEFSADDLKKLRKIVLFGGTAQILLTGLVIAMFS
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YWLMDAIGKSVGSKEALVLGFSFSVSSTALCLKILSDRGELGFDHGKIALGILIFQDMAI
HHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
VPLMFGFSFLTRGSSMPLESSFEEIALLLLFAIGMFGGFRLLMPKIVRIITELHAGEVLV
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
LGALVLCFGAAWLASLIGLSLALGAFMAGMVIASTDGSHRISRTIDPFREAMTSIFFVSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
GLLLDVNMIELPWLIAIALVVLVVKGLIMTGILMALGFSLRVSLMSGMVLAQIGEFSFVL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AGTAKDAGLLDQHMFQSMLAVIVVTMIVTPALISAAPKFAAQVAPALGFMPLASKPEPKQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCC
PARAAAGPIVCRGEIHAAIIGFGLIGRNIAAVMNATNLNYTVLDTDRKTVKTMRRQGEPL
CHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCE
FYGDCTERKSLLRIGVDHSRAVVICIPEIDAAIQCIRLVREINPGAFIIVRSRSLESTNR
EECCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
FYRAGADAAVTELFETSIQMFSELLKHFRVEPETILAQQEIIRREGGNIFRELVTETDGS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
GDDPAKKSQNGAGFVTSET
CCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MILLCFPGSEQGIGMHQFEFLGQLVLIGALAIVNILVFQKIRIPPVIGLIFTGIMLGPTG
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
FHVIRNSGLISTLAEMGVVLLLFTIGLEFSADDLKKLRKIVLFGGTAQILLTGLVIAMFS
HHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YWLMDAIGKSVGSKEALVLGFSFSVSSTALCLKILSDRGELGFDHGKIALGILIFQDMAI
HHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
VPLMFGFSFLTRGSSMPLESSFEEIALLLLFAIGMFGGFRLLMPKIVRIITELHAGEVLV
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHH
LGALVLCFGAAWLASLIGLSLALGAFMAGMVIASTDGSHRISRTIDPFREAMTSIFFVSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
GLLLDVNMIELPWLIAIALVVLVVKGLIMTGILMALGFSLRVSLMSGMVLAQIGEFSFVL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AGTAKDAGLLDQHMFQSMLAVIVVTMIVTPALISAAPKFAAQVAPALGFMPLASKPEPKQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCC
PARAAAGPIVCRGEIHAAIIGFGLIGRNIAAVMNATNLNYTVLDTDRKTVKTMRRQGEPL
CHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHCCCCE
FYGDCTERKSLLRIGVDHSRAVVICIPEIDAAIQCIRLVREINPGAFIIVRSRSLESTNR
EECCCHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
FYRAGADAAVTELFETSIQMFSELLKHFRVEPETILAQQEIIRREGGNIFRELVTETDGS
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
GDDPAKKSQNGAGFVTSET
CCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA