Click here to switch to the map view.

The map label for this gene is cysD [H]

Identifier: 21673439

GI number: 21673439

Start: 606400

End: 607689

Strand: Direct

Name: cysD [H]

Synonym: CT0604

Alternate gene names: 21673439

Gene position: 606400-607689 (Clockwise)

Preceding gene: 21673436

Following gene: 21673440

Centisome position: 28.14

GC content: 60.16

Gene sequence:

>1290_bases
ATGAGTGAGGATAACACCTTCCGGTTCGAGACCTTGCAGGTTCACGCCGGGCAGGAGCCTGATCCGGTGACCGGATCGCG
CGCCGTGCCCATTTACCAGACCACCTCCTACGTGTTCGAGAACGCCGAGCACGGCGCTGACCTGTTCGCGCTTCGCAAGG
CGGGCAATATCTACACGCGCCTGATGAACCCGACCACCGACGTGCTCGAAAAGCGCATGGCGGCGCTCGAAGGGGGCAAG
GCGGCCCTCGGCGTGGCGAGCGGCCACTCGGCGCAGTTCATCGCTATTGCCACCATCTGCCAGGCTGGAGACAACATTGT
GTCATCGAGCTATCTCTACGGCGGCACCTACAACCAGTTCAAGGTCGCCTTCAAGCGCCTCGGCATCGAGGTGAGGTTCG
TGGATGGCAACGATCAGGAGGCGTTCCGCAAGGCTATCGACGAGAACACGAAAGCGCTCTACATGGAGTCGATCGGCAAT
CCGGCGTTCCATGTGCCCGATTTCGACGCTATCGCGAAGATTGCCCGTGAGAACGGCATTCCGCTGATCGTCGATAACAC
CTTTGGCTGCGCGGGCTATCTCTGCCGTCCCATTGATCACGGCGCGTCGATCGTGGTCGAGTCGGCCACCAAGTGGATCG
GCGGGCACGGCACCTCGATGGGCGGCATCATCGTCGATGCCGGAACGTTCGACTGGGGCAACGGCAAGTTTCCGCTCTTT
ACCGAGCCATCGGAAGGCTATCACGGCCTGAAATTCTACGAAGCGGTCGGCGAGCTGGCCTTTATCATCCGGGCGCGGGT
CGAGGGACTGCGGGATTTCGGCCCGGCGATCAGCCCGTTCAACTCCTTCATGCTGTTGCAGGGACTTGAAACGCTCTCGC
TTCGCGTGCAGCGCCACCTCGACAACACGCTTGAACTGGCCCGCTGGCTCGAAAGGCACGATGCGGTTGCGTGGGTGAAC
TATCCAGGCCTCGAAAGCCATCCGACACACGCCCTGGCAAAAAAATATCTCACGCATGGCTTCGGCTGCGTGCTGACTTT
CGGCGTGAAGGGTGGTTATGAAAACGCGGTGAAGTTCATCGACAGCGTGAAGCTGGCGAGCCACCTGGCCAACGTGGGTG
ATGCAAAAACGCTCGTCATTCATCCGGCATCGACGACGCACCAGCAGCTCAGCGCCGAGGAACAGGTATCGGCGGGCGTC
ACCGCCGATATGGTGCGCGTGTCGGTTGGTATCGAGCATATCGATGACATCAAGGCTGATTTCAGCCAGGCTTTCGAGAA
TTTAGCATGA

Upstream 100 bases:

>100_bases
ATTCCTGCTGATTTCATACCTCACTTGATGCTTCCCGCACATACCTCCTGACCCCGACCGCGCACTACGGATCGAGCGCT
TCAACCTTGTACCATTTGCC

Downstream 100 bases:

>100_bases
CCCGGATGGACCATAGATCGATCATTTCCGACACTACACAATATTTTGAATCGAACGAGCCGTTACAGCTCGAACTGGGA
GGGGAGCTGCCCGGAGTGAG

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSEDNTFRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKAGNIYTRLMNPTTDVLEKRMAALEGGK
AALGVASGHSAQFIAIATICQAGDNIVSSSYLYGGTYNQFKVAFKRLGIEVRFVDGNDQEAFRKAIDENTKALYMESIGN
PAFHVPDFDAIAKIARENGIPLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
TEPSEGYHGLKFYEAVGELAFIIRARVEGLRDFGPAISPFNSFMLLQGLETLSLRVQRHLDNTLELARWLERHDAVAWVN
YPGLESHPTHALAKKYLTHGFGCVLTFGVKGGYENAVKFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQVSAGV
TADMVRVSVGIEHIDDIKADFSQAFENLA

Sequences:

>Translated_429_residues
MSEDNTFRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKAGNIYTRLMNPTTDVLEKRMAALEGGK
AALGVASGHSAQFIAIATICQAGDNIVSSSYLYGGTYNQFKVAFKRLGIEVRFVDGNDQEAFRKAIDENTKALYMESIGN
PAFHVPDFDAIAKIARENGIPLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
TEPSEGYHGLKFYEAVGELAFIIRARVEGLRDFGPAISPFNSFMLLQGLETLSLRVQRHLDNTLELARWLERHDAVAWVN
YPGLESHPTHALAKKYLTHGFGCVLTFGVKGGYENAVKFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQVSAGV
TADMVRVSVGIEHIDDIKADFSQAFENLA
>Mature_428_residues
SEDNTFRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKAGNIYTRLMNPTTDVLEKRMAALEGGKA
ALGVASGHSAQFIAIATICQAGDNIVSSSYLYGGTYNQFKVAFKRLGIEVRFVDGNDQEAFRKAIDENTKALYMESIGNP
AFHVPDFDAIAKIARENGIPLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLFT
EPSEGYHGLKFYEAVGELAFIIRARVEGLRDFGPAISPFNSFMLLQGLETLSLRVQRHLDNTLELARWLERHDAVAWVNY
PGLESHPTHALAKKYLTHGFGCVLTFGVKGGYENAVKFIDSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQVSAGVT
ADMVRVSVGIEHIDDIKADFSQAFENLA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=406, Percent_Identity=32.0197044334975, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI299473759, Length=406, Percent_Identity=28.8177339901478, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI25453487, Length=405, Percent_Identity=28.1481481481481, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790375, Length=423, Percent_Identity=29.3144208037825, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=24.2424242424242, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17538055, Length=423, Percent_Identity=34.2789598108747, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=423, Percent_Identity=34.0425531914894, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI71980708, Length=431, Percent_Identity=30.8584686774942, Blast_Score=197, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=59.7701149425287, Blast_Score=487, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6319307, Length=436, Percent_Identity=31.4220183486239, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=27.4157303370787, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=329, Percent_Identity=27.0516717325228, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=32.9411764705882, Blast_Score=203, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46677; Mature: 46546

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDNTFRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKAGNIYTR
CCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEEHHCCCEEEE
LMNPTTDVLEKRMAALEGGKAALGVASGHSAQFIAIATICQAGDNIVSSSYLYGGTYNQF
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHCCCCHHCCCEEECCCCHHH
KVAFKRLGIEVRFVDGNDQEAFRKAIDENTKALYMESIGNPAFHVPDFDAIAKIARENGI
HHHHHHHCEEEEEECCCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCHHHHHHHHHHCCC
PLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
EEEEECCCCCHHHHCCCCCCCCEEEEECHHHHHCCCCCCCCCEEEECCEEECCCCCCCEE
TEPSEGYHGLKFYEAVGELAFIIRARVEGLRDFGPAISPFNSFMLLQGLETLSLRVQRHL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DNTLELARWLERHDAVAWVNYPGLESHPTHALAKKYLTHGFGCVLTFGVKGGYENAVKFI
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
DSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQVSAGVTADMVRVSVGIEHIDDIKAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
FSQAFENLA
HHHHHHHCC
>Mature Secondary Structure 
SEDNTFRFETLQVHAGQEPDPVTGSRAVPIYQTTSYVFENAEHGADLFALRKAGNIYTR
CCCCCEEEEEEEEECCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCEEEEHHCCCEEEE
LMNPTTDVLEKRMAALEGGKAALGVASGHSAQFIAIATICQAGDNIVSSSYLYGGTYNQF
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHHHCCCCHHCCCEEECCCCHHH
KVAFKRLGIEVRFVDGNDQEAFRKAIDENTKALYMESIGNPAFHVPDFDAIAKIARENGI
HHHHHHHCEEEEEECCCCHHHHHHHHCCCCCEEEEHHCCCCCCCCCCHHHHHHHHHHCCC
PLIVDNTFGCAGYLCRPIDHGASIVVESATKWIGGHGTSMGGIIVDAGTFDWGNGKFPLF
EEEEECCCCCHHHHCCCCCCCCEEEEECHHHHHCCCCCCCCCEEEECCEEECCCCCCCEE
TEPSEGYHGLKFYEAVGELAFIIRARVEGLRDFGPAISPFNSFMLLQGLETLSLRVQRHL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DNTLELARWLERHDAVAWVNYPGLESHPTHALAKKYLTHGFGCVLTFGVKGGYENAVKFI
HHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHH
DSVKLASHLANVGDAKTLVIHPASTTHQQLSAEEQVSAGVTADMVRVSVGIEHIDDIKAD
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHCHHHHHHCCCHHHHHHHHHCHHHHHHHHHH
FSQAFENLA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]