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The map label for this gene is mrdA [H]

Identifier: 21673401

GI number: 21673401

Start: 573696

End: 575591

Strand: Reverse

Name: mrdA [H]

Synonym: CT0566

Alternate gene names: 21673401

Gene position: 575591-573696 (Counterclockwise)

Preceding gene: 21673402

Following gene: 21673400

Centisome position: 26.71

GC content: 59.02

Gene sequence:

>1896_bases
ATGGACGATTTTTTGCGCGGAATCAAGCTGACCATACTCCTCATCGCAGCCGTCTTCCTGTTTTTCACAGCTCGTCTCGC
CTGGCTCCAGATTGTCCAGCATGACGACATCAGTTCGAGATCGGGGAGCATCCGCAGAATCTGGGAGCAGGCGCCGAGGG
GCCATTTCATCGACCGGAATGGCATAACCGTCCTCGAAAACCAGGCACTCTACACCCTCAAGATCATCCCCAACGAGCTG
CGAGCATCGAGCATTCCGTACCTGGCCTACCTGCTTGAAATTCCGGTAGATGAGCTGAACGAGAAGGTCGCCGAAGCGAA
AGATTACAGTCCGTTTGCCGTTTCGACCATCTACCGCGACCTGAACGAGTTCGTCGTGGCACGCATCAGCGAGAACCTCT
GGCGATTGCCCGGCGTGATCATCGAGATCGAGAACAAGCGAAAATACAGCGATTTGTTCCGGGGAACCCACCTGTTCGGT
TACCTGCGCAATGTCAGCAAGGAGCAGCTCGACACGCTGGCTGAAAAGGGGTACACGCCGGACGACAAGATCGGTTTTTC
CGGTCTCGAACGCATCTACGAGGAGGAGCTTCGGGGCGAGAAGGGCGTGCGCTACGAGCTGGTCAATCCGCTCGGAATGC
TCATGGGAAAATACAATGAGGGCAAGAACGACATCCCGTCTGTCAAAGGCAACGATCTCTATCTCACCATCGACGCCCAC
CTGCAACGGCTTGCCGAAAACCTGCTCCGGGCGACCGGTCACCCCGGCGCGGTGGTGGCCATCGACCCCTCAGACGGCGG
CGTACTGGCGCTGTGCAGCGAACCCGATTTCGACCTCGACATCCTCAACGGCAAAACCAGAAAAAAAGAGTGGGCTGAAA
TCGCCCTTTCACCTGAAAAGCCCCTTTTCAACCGCGCCATCCAGGCCGTCTATCCGCCGGGTTCCACCTACAAACTGGTG
CTGGCCATCGCCGGACTTGAAGAGGGGGTCATCAAACCCGAAGACACCATCATCTCCACCGGCAGCTGGAACTACGGCGG
ACGCATCTTCCACGACCACGGCGGGCGCGGCCACGGCATCGTCAATATGAAAAAGGCGATCATCGAGTCCTGTAACATCT
ACTTCTACCAGCTCATGCTCAAGGTGGGCCTCGACACGTGGGACAAATACGGCAAAATGTTCGGATTCGGCCAGCGCGAG
GGCATCGACCTGCCGGGCGAACGGCGCGGCCTGTTGCCGACAACTGAGTACTACAACCGCCGCTACGGCGAGGGGCGCTG
GACAAAAGGCTATCTGGTAAGCCTTGGCATTGGCCAGGGTGAACTCGGCGTCACTCCCCTGCAACTGGCCAACTACGCAG
CGACCATCGCCAACAACGGCACCTGGCACCAACCGCACATTGTCAGGGGCTACCGCGACACGCGCACCGGCATCTATGTG
CCGATCGATCACGCCTCGCGCACCCTGCCAATCTCGAAAGAGACCTTCGGAATCATCAAGGAGGCGATGCAGGGCGTGGT
TCAGCAAGGAACCGGCACGCTGGCGCAGGTGCCGGGCGTGACGGTCGCCGGCAAAACCGGCACCGCGCAGAACCCGCACG
GCAAGGATCACGCCTGGTTCATCTGCTTCGCGCCGGTCGATCACCCGAAAATCGCCATCGCGGTGCTGGTCGAAAACGCC
GGTTTCGGCGGCAGCATCTCCGCGCCGATCGCCCGAGAGATGATAAACTACTACCTCGTCGAAAAGAACAAGCCGAAAAC
CCAGGGCGCCGACAGCACCGCTGTCGCCAAAATCAAAAAGCTGAACGATTCGCTAAACACGAAACGCCCCGCCGCGAAAA
CAGCAATCGACTCTACCAGCACTGCGCCGCAGAGCGATCTGGAGGGTGGAGACTGA

Upstream 100 bases:

>100_bases
CAGGGTACCGGAGACGGTCATCCTCGGCACCCTCATGAGCATGATGCTGGCCGTGCTCGTCTATCACTTCGCCCTGAAGA
AACTGCTGAAGGATTGAGCT

Downstream 100 bases:

>100_bases
TGAACTTCAAAAATGAACCAGCAGCCATTGCGGGCTTTCTCGAAGACACGAGCAACCTGAAAAACGGCTGGACGCCGGGC
GTCTTTTTCCCGGAAACGCC

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MDDFLRGIKLTILLIAAVFLFFTARLAWLQIVQHDDISSRSGSIRRIWEQAPRGHFIDRNGITVLENQALYTLKIIPNEL
RASSIPYLAYLLEIPVDELNEKVAEAKDYSPFAVSTIYRDLNEFVVARISENLWRLPGVIIEIENKRKYSDLFRGTHLFG
YLRNVSKEQLDTLAEKGYTPDDKIGFSGLERIYEEELRGEKGVRYELVNPLGMLMGKYNEGKNDIPSVKGNDLYLTIDAH
LQRLAENLLRATGHPGAVVAIDPSDGGVLALCSEPDFDLDILNGKTRKKEWAEIALSPEKPLFNRAIQAVYPPGSTYKLV
LAIAGLEEGVIKPEDTIISTGSWNYGGRIFHDHGGRGHGIVNMKKAIIESCNIYFYQLMLKVGLDTWDKYGKMFGFGQRE
GIDLPGERRGLLPTTEYYNRRYGEGRWTKGYLVSLGIGQGELGVTPLQLANYAATIANNGTWHQPHIVRGYRDTRTGIYV
PIDHASRTLPISKETFGIIKEAMQGVVQQGTGTLAQVPGVTVAGKTGTAQNPHGKDHAWFICFAPVDHPKIAIAVLVENA
GFGGSISAPIAREMINYYLVEKNKPKTQGADSTAVAKIKKLNDSLNTKRPAAKTAIDSTSTAPQSDLEGGD

Sequences:

>Translated_631_residues
MDDFLRGIKLTILLIAAVFLFFTARLAWLQIVQHDDISSRSGSIRRIWEQAPRGHFIDRNGITVLENQALYTLKIIPNEL
RASSIPYLAYLLEIPVDELNEKVAEAKDYSPFAVSTIYRDLNEFVVARISENLWRLPGVIIEIENKRKYSDLFRGTHLFG
YLRNVSKEQLDTLAEKGYTPDDKIGFSGLERIYEEELRGEKGVRYELVNPLGMLMGKYNEGKNDIPSVKGNDLYLTIDAH
LQRLAENLLRATGHPGAVVAIDPSDGGVLALCSEPDFDLDILNGKTRKKEWAEIALSPEKPLFNRAIQAVYPPGSTYKLV
LAIAGLEEGVIKPEDTIISTGSWNYGGRIFHDHGGRGHGIVNMKKAIIESCNIYFYQLMLKVGLDTWDKYGKMFGFGQRE
GIDLPGERRGLLPTTEYYNRRYGEGRWTKGYLVSLGIGQGELGVTPLQLANYAATIANNGTWHQPHIVRGYRDTRTGIYV
PIDHASRTLPISKETFGIIKEAMQGVVQQGTGTLAQVPGVTVAGKTGTAQNPHGKDHAWFICFAPVDHPKIAIAVLVENA
GFGGSISAPIAREMINYYLVEKNKPKTQGADSTAVAKIKKLNDSLNTKRPAAKTAIDSTSTAPQSDLEGGD
>Mature_631_residues
MDDFLRGIKLTILLIAAVFLFFTARLAWLQIVQHDDISSRSGSIRRIWEQAPRGHFIDRNGITVLENQALYTLKIIPNEL
RASSIPYLAYLLEIPVDELNEKVAEAKDYSPFAVSTIYRDLNEFVVARISENLWRLPGVIIEIENKRKYSDLFRGTHLFG
YLRNVSKEQLDTLAEKGYTPDDKIGFSGLERIYEEELRGEKGVRYELVNPLGMLMGKYNEGKNDIPSVKGNDLYLTIDAH
LQRLAENLLRATGHPGAVVAIDPSDGGVLALCSEPDFDLDILNGKTRKKEWAEIALSPEKPLFNRAIQAVYPPGSTYKLV
LAIAGLEEGVIKPEDTIISTGSWNYGGRIFHDHGGRGHGIVNMKKAIIESCNIYFYQLMLKVGLDTWDKYGKMFGFGQRE
GIDLPGERRGLLPTTEYYNRRYGEGRWTKGYLVSLGIGQGELGVTPLQLANYAATIANNGTWHQPHIVRGYRDTRTGIYV
PIDHASRTLPISKETFGIIKEAMQGVVQQGTGTLAQVPGVTVAGKTGTAQNPHGKDHAWFICFAPVDHPKIAIAVLVENA
GFGGSISAPIAREMINYYLVEKNKPKTQGADSTAVAKIKKLNDSLNTKRPAAKTAIDSTSTAPQSDLEGGD

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=616, Percent_Identity=31.0064935064935, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI1786272, Length=592, Percent_Identity=25.8445945945946, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69907; Mature: 69907

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDFLRGIKLTILLIAAVFLFFTARLAWLQIVQHDDISSRSGSIRRIWEQAPRGHFIDRN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEECCC
GITVLENQALYTLKIIPNELRASSIPYLAYLLEIPVDELNEKVAEAKDYSPFAVSTIYRD
CCEEEECCEEEEEEECCCHHCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
LNEFVVARISENLWRLPGVIIEIENKRKYSDLFRGTHLFGYLRNVSKEQLDTLAEKGYTP
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DDKIGFSGLERIYEEELRGEKGVRYELVNPLGMLMGKYNEGKNDIPSVKGNDLYLTIDAH
CCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHH
LQRLAENLLRATGHPGAVVAIDPSDGGVLALCSEPDFDLDILNGKTRKKEWAEIALSPEK
HHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCEEECCCCCCCCHHHHEECCCCC
PLFNRAIQAVYPPGSTYKLVLAIAGLEEGVIKPEDTIISTGSWNYGGRIFHDHGGRGHGI
CHHHHHHHEECCCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCC
VNMKKAIIESCNIYFYQLMLKVGLDTWDKYGKMFGFGQREGIDLPGERRGLLPTTEYYNR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHC
RYGEGRWTKGYLVSLGIGQGELGVTPLQLANYAATIANNGTWHQPHIVRGYRDTRTGIYV
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEE
PIDHASRTLPISKETFGIIKEAMQGVVQQGTGTLAQVPGVTVAGKTGTAQNPHGKDHAWF
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCCCCCCCEEE
ICFAPVDHPKIAIAVLVENAGFGGSISAPIAREMINYYLVEKNKPKTQGADSTAVAKIKK
EEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHH
LNDSLNTKRPAAKTAIDSTSTAPQSDLEGGD
HHHHHCCCCCCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDDFLRGIKLTILLIAAVFLFFTARLAWLQIVQHDDISSRSGSIRRIWEQAPRGHFIDRN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCEECCC
GITVLENQALYTLKIIPNELRASSIPYLAYLLEIPVDELNEKVAEAKDYSPFAVSTIYRD
CCEEEECCEEEEEEECCCHHCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHH
LNEFVVARISENLWRLPGVIIEIENKRKYSDLFRGTHLFGYLRNVSKEQLDTLAEKGYTP
HHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DDKIGFSGLERIYEEELRGEKGVRYELVNPLGMLMGKYNEGKNDIPSVKGNDLYLTIDAH
CCCCCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHH
LQRLAENLLRATGHPGAVVAIDPSDGGVLALCSEPDFDLDILNGKTRKKEWAEIALSPEK
HHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCEEECCCCCCCCHHHHEECCCCC
PLFNRAIQAVYPPGSTYKLVLAIAGLEEGVIKPEDTIISTGSWNYGGRIFHDHGGRGHGI
CHHHHHHHEECCCCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCCCC
VNMKKAIIESCNIYFYQLMLKVGLDTWDKYGKMFGFGQREGIDLPGERRGLLPTTEYYNR
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHC
RYGEGRWTKGYLVSLGIGQGELGVTPLQLANYAATIANNGTWHQPHIVRGYRDTRTGIYV
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEE
PIDHASRTLPISKETFGIIKEAMQGVVQQGTGTLAQVPGVTVAGKTGTAQNPHGKDHAWF
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCEEEECCCCCCCCCCCCCCEEE
ICFAPVDHPKIAIAVLVENAGFGGSISAPIAREMINYYLVEKNKPKTQGADSTAVAKIKK
EEECCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHH
LNDSLNTKRPAAKTAIDSTSTAPQSDLEGGD
HHHHHCCCCCCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]