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The map label for this gene is tyrS

Identifier: 21673398

GI number: 21673398

Start: 570484

End: 571704

Strand: Reverse

Name: tyrS

Synonym: CT0563

Alternate gene names: 21673398

Gene position: 571704-570484 (Counterclockwise)

Preceding gene: 21673399

Following gene: 21673397

Centisome position: 26.53

GC content: 58.48

Gene sequence:

>1221_bases
ATGATATTTCCATCCGTCAAGGAACAGCTCGATATCATCGTCAACAACACCGTCGAGGTCATCAGCACCGACGAACTCGA
ACGCAAACTCACCAAATCTCTCAAAAACGGCACGCCGCTGAAAATCAAGCTCGGTGCTGACCCGTCGCGCCCCGACCTGC
ACCTCGGCCACTCGGTGGTGCTCCGCAAGCTGCGTGAATTTCAGGATTTGGGACACGAAGCGATCCTCATCATCGGCGAC
TTCACAGCCATGATCGGCGACCCGTCAGGCAAGAGCAAGACCCGCCCGCAGCTCACCGCCGAAGAGGCCCGCGAGAACGG
CAAAAGCTACTTCGAGCAGGCGTCGAAAATCCTCGATCCGGAAAAGACCACCATCTGCTACAACGCCGACTGGCTGAGCC
AGATGCACTTTGCCGACGTCATCCGCTTGTCGAGCCACTACACGGTGGCGCGGATGCTGGAGCGCGATGATTTCGAAAAG
CGCTACCGCTCGCAGACCCCGATCTCGATCCACGAATTTCTCTATCCGCTCGCACAGGGCATGGACTCGGTGCACCTCAA
AAATGATGTGGAGCTTGGCGGCACCGACCAGAAGTTCAACCTGCTCGTTGGGCGCGACCTCCAGCGCGAGTACGGCATCG
AGCCGCAGGTGTGCATCACCATGCCGCTGCTGGTGGGCACCGACGGCAGCGAGAAGATGTCCAAGTCACTCGGCAACGCC
ATCTGCTTCAACGACACGCCGGAGGATATGTACGGTCGAACGCTCTCAATTCCCGACTCCCTGATCGAGACCTACTGGAA
CCTGCTGGTACCGCACCACAGCGGCAACGACAAGCCTATCGCCGAACGAATCGCCGCCGATCCACGCGAAACCAAGCGAG
AACTCGCCCGCGAACTGGTCGCGCAATATTATTCGGCGGAGGAGGCGGCCAAGGCACAGGAGCACTTTGACCGGGTGATC
GTCAACAAACAGGCGCCCACAGACCTGCCGACCGTGGAGTTCGAGGAGGCTACGATGCCGGTGGTCGAGCTGCTCATGGC
GCTTGCGGCATTCCCGTCCAAAAACGAAGCACGGCGCATGATTCAGCAGGGCGCGGTGCAGGCTGGCAACGAGAAAATTT
CTGATATCAATGCCGTTATCGAGCTGACCGAAAACCCCGTCATCATCCGCGCAGGCAAACGAAAATTTTTCAAGGTGGCG
CGCGCAAAAAAATCGTTTTGA

Upstream 100 bases:

>100_bases
AAGCTCTACGACAAGCGCGAGACAATCAAGAATCGAGACGCCAAGCGTCAGCTGCAGCAACTCAGGAAACAGTATTAACC
AGACGACATTTCAATTTGCC

Downstream 100 bases:

>100_bases
ATGCGTTTGCTTTTTCCTATAATTGCACCAACTGACGCCTTTCTCTAACCTCTATCCGGAGCTACCACCTTGTCATTCGT
CCCAACAAAAGTATTCTTCA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MIFPSVKEQLDIIVNNTVEVISTDELERKLTKSLKNGTPLKIKLGADPSRPDLHLGHSVVLRKLREFQDLGHEAILIIGD
FTAMIGDPSGKSKTRPQLTAEEARENGKSYFEQASKILDPEKTTICYNADWLSQMHFADVIRLSSHYTVARMLERDDFEK
RYRSQTPISIHEFLYPLAQGMDSVHLKNDVELGGTDQKFNLLVGRDLQREYGIEPQVCITMPLLVGTDGSEKMSKSLGNA
ICFNDTPEDMYGRTLSIPDSLIETYWNLLVPHHSGNDKPIAERIAADPRETKRELARELVAQYYSAEEAAKAQEHFDRVI
VNKQAPTDLPTVEFEEATMPVVELLMALAAFPSKNEARRMIQQGAVQAGNEKISDINAVIELTENPVIIRAGKRKFFKVA
RAKKSF

Sequences:

>Translated_406_residues
MIFPSVKEQLDIIVNNTVEVISTDELERKLTKSLKNGTPLKIKLGADPSRPDLHLGHSVVLRKLREFQDLGHEAILIIGD
FTAMIGDPSGKSKTRPQLTAEEARENGKSYFEQASKILDPEKTTICYNADWLSQMHFADVIRLSSHYTVARMLERDDFEK
RYRSQTPISIHEFLYPLAQGMDSVHLKNDVELGGTDQKFNLLVGRDLQREYGIEPQVCITMPLLVGTDGSEKMSKSLGNA
ICFNDTPEDMYGRTLSIPDSLIETYWNLLVPHHSGNDKPIAERIAADPRETKRELARELVAQYYSAEEAAKAQEHFDRVI
VNKQAPTDLPTVEFEEATMPVVELLMALAAFPSKNEARRMIQQGAVQAGNEKISDINAVIELTENPVIIRAGKRKFFKVA
RAKKSF
>Mature_406_residues
MIFPSVKEQLDIIVNNTVEVISTDELERKLTKSLKNGTPLKIKLGADPSRPDLHLGHSVVLRKLREFQDLGHEAILIIGD
FTAMIGDPSGKSKTRPQLTAEEARENGKSYFEQASKILDPEKTTICYNADWLSQMHFADVIRLSSHYTVARMLERDDFEK
RYRSQTPISIHEFLYPLAQGMDSVHLKNDVELGGTDQKFNLLVGRDLQREYGIEPQVCITMPLLVGTDGSEKMSKSLGNA
ICFNDTPEDMYGRTLSIPDSLIETYWNLLVPHHSGNDKPIAERIAADPRETKRELARELVAQYYSAEEAAKAQEHFDRVI
VNKQAPTDLPTVEFEEATMPVVELLMALAAFPSKNEARRMIQQGAVQAGNEKISDINAVIELTENPVIIRAGKRKFFKVA
RAKKSF

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=404, Percent_Identity=25.4950495049505, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787925, Length=405, Percent_Identity=26.1728395061728, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25145802, Length=222, Percent_Identity=30.1801801801802, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325160, Length=424, Percent_Identity=26.6509433962264, Blast_Score=114, Evalue=4e-26,
Organism=Drosophila melanogaster, GI20130383, Length=409, Percent_Identity=27.1393643031785, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_CHLTE (Q8KEW9)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661463.1
- ProteinModelPortal:   Q8KEW9
- SMR:   Q8KEW9
- GeneID:   1007613
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0563
- NMPDR:   fig|194439.1.peg.557
- TIGR:   CT0563
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   CTEP194439:CT_0563-MONOMER
- BRENDA:   6.1.1.1
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45725; Mature: 45725

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFPSVKEQLDIIVNNTVEVISTDELERKLTKSLKNGTPLKIKLGADPSRPDLHLGHSVV
CCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHH
LRKLREFQDLGHEAILIIGDFTAMIGDPSGKSKTRPQLTAEEARENGKSYFEQASKILDP
HHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EKTTICYNADWLSQMHFADVIRLSSHYTVARMLERDDFEKRYRSQTPISIHEFLYPLAQG
CCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
MDSVHLKNDVELGGTDQKFNLLVGRDLQREYGIEPQVCITMPLLVGTDGSEKMSKSLGNA
CCCEECCCCCCCCCCCCEEEEEECCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHCCE
ICFNDTPEDMYGRTLSIPDSLIETYWNLLVPHHSGNDKPIAERIAADPRETKRELARELV
EECCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
AQYYSAEEAAKAQEHFDRVIVNKQAPTDLPTVEFEEATMPVVELLMALAAFPSKNEARRM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
IQQGAVQAGNEKISDINAVIELTENPVIIRAGKRKFFKVARAKKSF
HHHHHHHCCCHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIFPSVKEQLDIIVNNTVEVISTDELERKLTKSLKNGTPLKIKLGADPSRPDLHLGHSVV
CCCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHH
LRKLREFQDLGHEAILIIGDFTAMIGDPSGKSKTRPQLTAEEARENGKSYFEQASKILDP
HHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
EKTTICYNADWLSQMHFADVIRLSSHYTVARMLERDDFEKRYRSQTPISIHEFLYPLAQG
CCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
MDSVHLKNDVELGGTDQKFNLLVGRDLQREYGIEPQVCITMPLLVGTDGSEKMSKSLGNA
CCCEECCCCCCCCCCCCEEEEEECCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHCCE
ICFNDTPEDMYGRTLSIPDSLIETYWNLLVPHHSGNDKPIAERIAADPRETKRELARELV
EECCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHH
AQYYSAEEAAKAQEHFDRVIVNKQAPTDLPTVEFEEATMPVVELLMALAAFPSKNEARRM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
IQQGAVQAGNEKISDINAVIELTENPVIIRAGKRKFFKVARAKKSF
HHHHHHHCCCHHHHHHHHHEEECCCCEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901