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The map label for this gene is nadB [H]

Identifier: 21673396

GI number: 21673396

Start: 568039

End: 569634

Strand: Reverse

Name: nadB [H]

Synonym: CT0561

Alternate gene names: 21673396

Gene position: 569634-568039 (Counterclockwise)

Preceding gene: 21673397

Following gene: 21673391

Centisome position: 26.43

GC content: 60.03

Gene sequence:

>1596_bases
ATGACACAGGAAGTGAAAACCGACGTGCTGGTTATCGGCAGCGGCATCGGAGGGTTGTATTTCGCCATCAATATGGCCGA
CCACGCCACGGTCACGATCATCACCAAAAAAGAGAGCTCCACCTCCAATACCAACTGGGCACAGGGCGGCATCGCGGCGG
CCATCGCGGGTGACGACACCCCGGAGCTGCACATCGCTGACACGCTCGATGCGGGCGCGGGACTGTGCAACGAGGCAATA
GTGAGCATCCTGGTGCACGAAGGCCCAGCGCACATCCGCCGACTCATCGAGCTTGGCGTGGAGTTCACGACCAACCCCGA
CCATACGCTCAACCTCGGCAAGGAAGGTGGCCATTCGCGCAACCGCATCGTGCACGCCAAAGATTTGACGGGCCGCGAGG
TGGAGAGGGCGCTCCTCGCGCGGGCCAACGCGCACCCCAACATCACGCTGCTGGAGCACCACTACGCGCTCGAACTCATC
ACCGAGCACCACCTCGGCATCAAGACCAACGACATCACCTGCTACGGCGCGTACGTACTCGACACGCTCAACCACAAGCC
AAAAAAGATTCTGGCGAAGGTAACGATGGTCGCCTCGGGCGGGCTGGGCCACGTCTATCTGCATACGACCAATCCCGAGA
TCGCCACGGGCGACGGCATCGCAATGGCCTACCGCGCCGGAGCCGAGATCGCCAACATGGAGTTCATCCAGTTCCACCCG
ACCTCACTCTTCCACCCGAAAGCGAAATCGTTCCTGATCTCGGAGGCGGTGCGCGGCTTCGGCGGCATTTTGCGCAACAA
GGAGGGCGAGGCGTTCATGCACCGCTACGACCGGCGCGAAAATCTCGCCCCGCGCGACATCGTGGCGCGAGCGATCGACT
CGGAGATGAAAAAGAACGGCGACGAGTGCGTCTTTCTCGACGTGACGCACATCAAGGCGGAGAAGGTCAGGGAGCACTTC
CCGCACATTTACGAGACCTGCCTCGGTTTCGGCATCGACATGACAAAGGAGATGATTCCAGTGGTTCCAGCGGCGCACTA
CTCATGCGGCGGCATCAGAACGGACAGCTGGGGGCGCAGCACCATCAACCACCTCTATGCCTGCGGCGAGACAAGCTGCA
CTGGCGTACACGGCGCAAACCGGCTGGCGAGCAACTCGTTGCTCGAAGCGCTGGTTTTCGCCTGGCGCTCCAGCGAAGAT
ATCCGGGCGGAACTGAAATCGATTCATTTCAAGCACGAATTCCCCGACTGGGACGACTCCGGCACAACCAGCCCGGAGGA
GTGGATCCTGGTGGCGCACAACAAAAAAGAGGCGCAGGTGATCATGAACGACTACGTCGGCATCGTGAGGAGCGACCTGC
GCCTCGACCGCGCCCGACGGCGCATCGATTTCCTGAAGGAGGAGACCGAGGCTTACTACAAAAAAACGAAGATCACGCCA
CAGATCATCGAACTGCGCAATATCATCAAGGTTGCAAGCCTCATCATTCAGGGAGCAATCAAGCGCCGCGAATCACGGGG
CCTGCATTACACAACAGATTTCCCGCAGAAGGACGACAAGCATTATCTGGCGGACACCGTGCTCCGGTCGTTTTGA

Upstream 100 bases:

>100_bases
ACTGTGTATATTACGCCTCGCCGTTTTTGTTTCGGGAAAACAGCGGGCTGTCTCTGGGCTACTCTACCCGCAACTCATCA
AACACGCCACACAGACTTAT

Downstream 100 bases:

>100_bases
GAAGTGGGATTTTCGGGACTTTTGGGAAAAAAGATCAGGGAATGTTTCCCAAAGCTCCACAGGCATCACCGGCCCATGCA
AACGCTCCCCTACCGCACCT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTQEVKTDVLVIGSGIGGLYFAINMADHATVTIITKKESSTSNTNWAQGGIAAAIAGDDTPELHIADTLDAGAGLCNEAI
VSILVHEGPAHIRRLIELGVEFTTNPDHTLNLGKEGGHSRNRIVHAKDLTGREVERALLARANAHPNITLLEHHYALELI
TEHHLGIKTNDITCYGAYVLDTLNHKPKKILAKVTMVASGGLGHVYLHTTNPEIATGDGIAMAYRAGAEIANMEFIQFHP
TSLFHPKAKSFLISEAVRGFGGILRNKEGEAFMHRYDRRENLAPRDIVARAIDSEMKKNGDECVFLDVTHIKAEKVREHF
PHIYETCLGFGIDMTKEMIPVVPAAHYSCGGIRTDSWGRSTINHLYACGETSCTGVHGANRLASNSLLEALVFAWRSSED
IRAELKSIHFKHEFPDWDDSGTTSPEEWILVAHNKKEAQVIMNDYVGIVRSDLRLDRARRRIDFLKEETEAYYKKTKITP
QIIELRNIIKVASLIIQGAIKRRESRGLHYTTDFPQKDDKHYLADTVLRSF

Sequences:

>Translated_531_residues
MTQEVKTDVLVIGSGIGGLYFAINMADHATVTIITKKESSTSNTNWAQGGIAAAIAGDDTPELHIADTLDAGAGLCNEAI
VSILVHEGPAHIRRLIELGVEFTTNPDHTLNLGKEGGHSRNRIVHAKDLTGREVERALLARANAHPNITLLEHHYALELI
TEHHLGIKTNDITCYGAYVLDTLNHKPKKILAKVTMVASGGLGHVYLHTTNPEIATGDGIAMAYRAGAEIANMEFIQFHP
TSLFHPKAKSFLISEAVRGFGGILRNKEGEAFMHRYDRRENLAPRDIVARAIDSEMKKNGDECVFLDVTHIKAEKVREHF
PHIYETCLGFGIDMTKEMIPVVPAAHYSCGGIRTDSWGRSTINHLYACGETSCTGVHGANRLASNSLLEALVFAWRSSED
IRAELKSIHFKHEFPDWDDSGTTSPEEWILVAHNKKEAQVIMNDYVGIVRSDLRLDRARRRIDFLKEETEAYYKKTKITP
QIIELRNIIKVASLIIQGAIKRRESRGLHYTTDFPQKDDKHYLADTVLRSF
>Mature_530_residues
TQEVKTDVLVIGSGIGGLYFAINMADHATVTIITKKESSTSNTNWAQGGIAAAIAGDDTPELHIADTLDAGAGLCNEAIV
SILVHEGPAHIRRLIELGVEFTTNPDHTLNLGKEGGHSRNRIVHAKDLTGREVERALLARANAHPNITLLEHHYALELIT
EHHLGIKTNDITCYGAYVLDTLNHKPKKILAKVTMVASGGLGHVYLHTTNPEIATGDGIAMAYRAGAEIANMEFIQFHPT
SLFHPKAKSFLISEAVRGFGGILRNKEGEAFMHRYDRRENLAPRDIVARAIDSEMKKNGDECVFLDVTHIKAEKVREHFP
HIYETCLGFGIDMTKEMIPVVPAAHYSCGGIRTDSWGRSTINHLYACGETSCTGVHGANRLASNSLLEALVFAWRSSEDI
RAELKSIHFKHEFPDWDDSGTTSPEEWILVAHNKKEAQVIMNDYVGIVRSDLRLDRARRRIDFLKEETEAYYKKTKITPQ
IIELRNIIKVASLIIQGAIKRRESRGLHYTTDFPQKDDKHYLADTVLRSF

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=535, Percent_Identity=31.214953271028, Blast_Score=212, Evalue=6e-55,
Organism=Escherichia coli, GI1788928, Length=517, Percent_Identity=47.1953578336557, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1790597, Length=543, Percent_Identity=31.4917127071823, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1786942, Length=546, Percent_Identity=29.4871794871795, Blast_Score=198, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17550100, Length=541, Percent_Identity=30.6839186691312, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=531, Percent_Identity=30.6967984934087, Blast_Score=202, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI71986328, Length=472, Percent_Identity=26.9067796610169, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322701, Length=536, Percent_Identity=30.5970149253731, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322416, Length=538, Percent_Identity=30.8550185873606, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6320788, Length=480, Percent_Identity=27.5, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=344, Percent_Identity=27.3255813953488, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24663005, Length=566, Percent_Identity=31.8021201413428, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI17137288, Length=556, Percent_Identity=30.5755395683453, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24655642, Length=556, Percent_Identity=30.5755395683453, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24655647, Length=556, Percent_Identity=30.5755395683453, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59105; Mature: 58974

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEVKTDVLVIGSGIGGLYFAINMADHATVTIITKKESSTSNTNWAQGGIAAAIAGDDT
CCCCCCCCEEEEECCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCC
PELHIADTLDAGAGLCNEAIVSILVHEGPAHIRRLIELGVEFTTNPDHTLNLGKEGGHSR
CCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCEEECCCCCCCCC
NRIVHAKDLTGREVERALLARANAHPNITLLEHHYALELITEHHLGIKTNDITCYGAYVL
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEEECCEEEEHHHHH
DTLNHKPKKILAKVTMVASGGLGHVYLHTTNPEIATGDGIAMAYRAGAEIANMEFIQFHP
HHCCCCHHHHHHHHHHEECCCCCEEEEEECCCCEECCCCEEEEECCCCCCCCEEEEEECC
TSLFHPKAKSFLISEAVRGFGGILRNKEGEAFMHRYDRRENLAPRDIVARAIDSEMKKNG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
DECVFLDVTHIKAEKVREHFPHIYETCLGFGIDMTKEMIPVVPAAHYSCGGIRTDSWGRS
CCEEEEEEHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEECCCCCHH
TINHLYACGETSCTGVHGANRLASNSLLEALVFAWRSSEDIRAELKSIHFKHEFPDWDDS
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCC
GTTSPEEWILVAHNKKEAQVIMNDYVGIVRSDLRLDRARRRIDFLKEETEAYYKKTKITP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QIIELRNIIKVASLIIQGAIKRRESRGLHYTTDFPQKDDKHYLADTVLRSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TQEVKTDVLVIGSGIGGLYFAINMADHATVTIITKKESSTSNTNWAQGGIAAAIAGDDT
CCCCCCCEEEEECCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCCC
PELHIADTLDAGAGLCNEAIVSILVHEGPAHIRRLIELGVEFTTNPDHTLNLGKEGGHSR
CCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCEEECCCCCCCCC
NRIVHAKDLTGREVERALLARANAHPNITLLEHHYALELITEHHLGIKTNDITCYGAYVL
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCEEECCEEEEHHHHH
DTLNHKPKKILAKVTMVASGGLGHVYLHTTNPEIATGDGIAMAYRAGAEIANMEFIQFHP
HHCCCCHHHHHHHHHHEECCCCCEEEEEECCCCEECCCCEEEEECCCCCCCCEEEEEECC
TSLFHPKAKSFLISEAVRGFGGILRNKEGEAFMHRYDRRENLAPRDIVARAIDSEMKKNG
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
DECVFLDVTHIKAEKVREHFPHIYETCLGFGIDMTKEMIPVVPAAHYSCGGIRTDSWGRS
CCEEEEEEHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCEECCCCCHH
TINHLYACGETSCTGVHGANRLASNSLLEALVFAWRSSEDIRAELKSIHFKHEFPDWDDS
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCC
GTTSPEEWILVAHNKKEAQVIMNDYVGIVRSDLRLDRARRRIDFLKEETEAYYKKTKITP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
QIIELRNIIKVASLIIQGAIKRRESRGLHYTTDFPQKDDKHYLADTVLRSF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]