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The map label for this gene is glnB [H]

Identifier: 21673334

GI number: 21673334

Start: 512632

End: 512985

Strand: Reverse

Name: glnB [H]

Synonym: CT0499

Alternate gene names: 21673334

Gene position: 512985-512632 (Counterclockwise)

Preceding gene: 21673335

Following gene: 21673333

Centisome position: 23.81

GC content: 54.52

Gene sequence:

>354_bases
ATGAAAAAAATAGAAGCCATCATTCGCACAAGCCAGTACGAAGCGGTCAAAAAAGCCCTGCATGAGATCGACATCGATTT
CTTCACCTGGTGGGACGTGACTGGGCAGGGCAACGACAAGAAGCACCGCACCGAGATCTACCGGGGCACCGTCAGGGACA
CCTCGTACATCTCGCGCCGCTACCTGTCGATTGTCGTTCGCGACGTCAATCTCCAGAAAACGGTCGATTGCCTGATCAAA
AACGCCCGGACGGGTGAGGTGGGAGACGGAAAAATTTTCGTTTCGGACATCGAGCAGTCCTACCGGATCCGAACGGGAGA
GAGCGGCGACGAATCGCTGTACAACAAGGACTAG

Upstream 100 bases:

>100_bases
GGAGGAGACCTATGAAGTACGATTGAACAACCTTGCCAACGCGGCTCGCTACCGAGCCATTTCGTTCTACTATCAAACAA
TTAACGCAGTCTTTAAACCC

Downstream 100 bases:

>100_bases
GATTAAAACACAAAAAAATTATCACCAATTCATGTAAGTCAACAACCACCATGGTACAAAAACTGATTTTCATATTGACC
GGAGTCCTCCTGATGCTGGG

Product: P-II family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MKKIEAIIRTSQYEAVKKALHEIDIDFFTWWDVTGQGNDKKHRTEIYRGTVRDTSYISRRYLSIVVRDVNLQKTVDCLIK
NARTGEVGDGKIFVSDIEQSYRIRTGESGDESLYNKD

Sequences:

>Translated_117_residues
MKKIEAIIRTSQYEAVKKALHEIDIDFFTWWDVTGQGNDKKHRTEIYRGTVRDTSYISRRYLSIVVRDVNLQKTVDCLIK
NARTGEVGDGKIFVSDIEQSYRIRTGESGDESLYNKD
>Mature_117_residues
MKKIEAIIRTSQYEAVKKALHEIDIDFFTWWDVTGQGNDKKHRTEIYRGTVRDTSYISRRYLSIVVRDVNLQKTVDCLIK
NARTGEVGDGKIFVSDIEQSYRIRTGESGDESLYNKD

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=113, Percent_Identity=42.4778761061947, Blast_Score=87, Evalue=4e-19,
Organism=Escherichia coli, GI1786655, Length=113, Percent_Identity=36.283185840708, Blast_Score=78, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 13594; Mature: 13594

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIEAIIRTSQYEAVKKALHEIDIDFFTWWDVTGQGNDKKHRTEIYRGTVRDTSYISRR
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHH
YLSIVVRDVNLQKTVDCLIKNARTGEVGDGKIFVSDIEQSYRIRTGESGDESLYNKD
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKKIEAIIRTSQYEAVKKALHEIDIDFFTWWDVTGQGNDKKHRTEIYRGTVRDTSYISRR
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHHH
YLSIVVRDVNLQKTVDCLIKNARTGEVGDGKIFVSDIEQSYRIRTGESGDESLYNKD
HHHHHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]