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The map label for this gene is amt-1 [C]

Identifier: 21673333

GI number: 21673333

Start: 511031

End: 512581

Strand: Reverse

Name: amt-1 [C]

Synonym: CT0498

Alternate gene names: 21673333

Gene position: 512581-511031 (Counterclockwise)

Preceding gene: 21673334

Following gene: 21673332

Centisome position: 23.79

GC content: 60.28

Gene sequence:

>1551_bases
ATGGTACAAAAACTGATTTTCATATTGACCGGAGTCCTCCTGATGCTGGGCATTGCGATGGGGGGTGATCTCCATGCCTA
CACGCAGCCCGATCCGTCGGGAGCCAAGGTCGGCGTGACCGCCGACATCTCGGCGGCAAATCCGGGAAAGCCAACCGCCG
CAGAAATCACCGACCAGATCGGCAAGAACAAGGTCTCGATCAACATGGTGTGGGTGCTCATCACCGGCTTCCTCGTCATG
TTCATGCAGGCCGGATTCGCGATGGTAGAGGCGGGCCTGACCAGGGCCAAGAACGCCGCGCACACCATGTCGATGAACAT
GATGATCTATCCAATCGGTATGCTCGGCTTTTTCGTGAGCGGTTTCGCCATCATGTTCGGCGGCCTCGGAACGCTCGGAA
CGCTGGGCGGTTATGAGGGACTCAATCAGGAGTTCGCCATCAACCTGTTCGGGCACACCATCGGCCTGTTCGGCATGAAA
GGTTTCTTTCTGAACAATGTGTATGATGTCGGCGTGTTCGCGCTCTTCCTCTTCCAGATGGTCTTCATGGACACCACCGC
CACCATCCCGACCGGCTCGATGGCTGAGCGCTGGAAGTTCTCGGCCTTCGCGATCTACGGCATTTTCGTTGGCACCATCA
TCTATCCGCTTTACGGCAACTGGGTTTGGGGCGGCGGCTGGCTGGCCACGCTCGGCTCGAATTTCGGCCTTGGTCACGGC
CACGTTGACTTTGCAGGCTCCTCGGTGGTGCACATGACTGGCGGCGTGCTGGCTCTGGTCGGCGCGATCATCATCGGCCC
CCGCATCGGCAAGTACAACAAGGACGGCTCGCCCAACGCGATTCCCGGCCACAACATTCCGATGGCCATCGTCGGCGCGT
TCATTCTCGCTTTCGGCTGGTTCGGCTTCAACCCTGGCTCGACGCTCGCGGGCACTGACCTGCGCATCAGCGTGGTGGCG
GTCAACACCATGATCGCCTCGGCGACCGGCGCGGCGGCCTCGATGCTCTACATGTGGCTTTTCAAAACGAAAAAGCCCGA
CCCGACCATGATGATCAACGGCTTGCTCGCCGGTCTGGTGGCGATCACCGCTCCATCGGCTTTCGTCAACGTGCAGGCGG
CGGCGCTCATCGGCCTGATCTCCGGCGTGCTGGTGATCGAAGCGGCCTTCTTCATCGAGAAGGTGCTCAAGATTGACGAC
CCGGTCGGCGCGGTCGCCGTGCACGGCGTGAACGGCGCGTGGGGCTGTCTGGCGCTCGGTCTGTTCGCTGACGGCACCTA
CGGCGACGGATGGAACGGCGTTGCCGGTCCGGTCACCGGCCTGTTCTACGGCAACCCCGGACAGTTCTATGCGGAAGTGA
TCGGCGTGCTCGCCAACATCATCTACGTCGGAGCTATCGGCTGGGTAGTCTTCAAGCTGATCGACGTCACCATCGGCAAC
CGCGTCAACCCGGATGATGAACTCGACGGTCTCGACATTCCTGAAATGGGTGTCGAGGGCTACTGCGGCATCAGGCTCGA
CAAAAATTCCGAGACTCCGCTTTCGAAATAA

Upstream 100 bases:

>100_bases
GGATCCGAACGGGAGAGAGCGGCGACGAATCGCTGTACAACAAGGACTAGGATTAAAACACAAAAAAATTATCACCAATT
CATGTAAGTCAACAACCACC

Downstream 100 bases:

>100_bases
TCCTTCCATACAACATGTTCATCACGACGCCGCCTTTTACCGGGCGGCGTCTTTGTTTCAGCCGCTCGTTTCAAACCAGA
AATCACCGTATTGGCATCAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MVQKLIFILTGVLLMLGIAMGGDLHAYTQPDPSGAKVGVTADISAANPGKPTAAEITDQIGKNKVSINMVWVLITGFLVM
FMQAGFAMVEAGLTRAKNAAHTMSMNMMIYPIGMLGFFVSGFAIMFGGLGTLGTLGGYEGLNQEFAINLFGHTIGLFGMK
GFFLNNVYDVGVFALFLFQMVFMDTTATIPTGSMAERWKFSAFAIYGIFVGTIIYPLYGNWVWGGGWLATLGSNFGLGHG
HVDFAGSSVVHMTGGVLALVGAIIIGPRIGKYNKDGSPNAIPGHNIPMAIVGAFILAFGWFGFNPGSTLAGTDLRISVVA
VNTMIASATGAAASMLYMWLFKTKKPDPTMMINGLLAGLVAITAPSAFVNVQAAALIGLISGVLVIEAAFFIEKVLKIDD
PVGAVAVHGVNGAWGCLALGLFADGTYGDGWNGVAGPVTGLFYGNPGQFYAEVIGVLANIIYVGAIGWVVFKLIDVTIGN
RVNPDDELDGLDIPEMGVEGYCGIRLDKNSETPLSK

Sequences:

>Translated_516_residues
MVQKLIFILTGVLLMLGIAMGGDLHAYTQPDPSGAKVGVTADISAANPGKPTAAEITDQIGKNKVSINMVWVLITGFLVM
FMQAGFAMVEAGLTRAKNAAHTMSMNMMIYPIGMLGFFVSGFAIMFGGLGTLGTLGGYEGLNQEFAINLFGHTIGLFGMK
GFFLNNVYDVGVFALFLFQMVFMDTTATIPTGSMAERWKFSAFAIYGIFVGTIIYPLYGNWVWGGGWLATLGSNFGLGHG
HVDFAGSSVVHMTGGVLALVGAIIIGPRIGKYNKDGSPNAIPGHNIPMAIVGAFILAFGWFGFNPGSTLAGTDLRISVVA
VNTMIASATGAAASMLYMWLFKTKKPDPTMMINGLLAGLVAITAPSAFVNVQAAALIGLISGVLVIEAAFFIEKVLKIDD
PVGAVAVHGVNGAWGCLALGLFADGTYGDGWNGVAGPVTGLFYGNPGQFYAEVIGVLANIIYVGAIGWVVFKLIDVTIGN
RVNPDDELDGLDIPEMGVEGYCGIRLDKNSETPLSK
>Mature_516_residues
MVQKLIFILTGVLLMLGIAMGGDLHAYTQPDPSGAKVGVTADISAANPGKPTAAEITDQIGKNKVSINMVWVLITGFLVM
FMQAGFAMVEAGLTRAKNAAHTMSMNMMIYPIGMLGFFVSGFAIMFGGLGTLGTLGGYEGLNQEFAINLFGHTIGLFGMK
GFFLNNVYDVGVFALFLFQMVFMDTTATIPTGSMAERWKFSAFAIYGIFVGTIIYPLYGNWVWGGGWLATLGSNFGLGHG
HVDFAGSSVVHMTGGVLALVGAIIIGPRIGKYNKDGSPNAIPGHNIPMAIVGAFILAFGWFGFNPGSTLAGTDLRISVVA
VNTMIASATGAAASMLYMWLFKTKKPDPTMMINGLLAGLVAITAPSAFVNVQAAALIGLISGVLVIEAAFFIEKVLKIDD
PVGAVAVHGVNGAWGCLALGLFADGTYGDGWNGVAGPVTGLFYGNPGQFYAEVIGVLANIIYVGAIGWVVFKLIDVTIGN
RVNPDDELDGLDIPEMGVEGYCGIRLDKNSETPLSK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=434, Percent_Identity=31.7972350230415, Blast_Score=174, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17550182, Length=449, Percent_Identity=31.8485523385301, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17550180, Length=465, Percent_Identity=30.752688172043, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI32564042, Length=448, Percent_Identity=29.6875, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI133931100, Length=449, Percent_Identity=29.6213808463252, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324187, Length=441, Percent_Identity=27.2108843537415, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325396, Length=444, Percent_Identity=26.5765765765766, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321559, Length=442, Percent_Identity=25.7918552036199, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078314, Length=441, Percent_Identity=33.7868480725624, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 54171; Mature: 54171

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQKLIFILTGVLLMLGIAMGGDLHAYTQPDPSGAKVGVTADISAANPGKPTAAEITDQI
CHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
GKNKVSINMVWVLITGFLVMFMQAGFAMVEAGLTRAKNAAHTMSMNMMIYPIGMLGFFVS
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECEEEEHHHHHHHHHH
GFAIMFGGLGTLGTLGGYEGLNQEFAINLFGHTIGLFGMKGFFLNNVYDVGVFALFLFQM
HHHHHHCCCHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFMDTTATIPTGSMAERWKFSAFAIYGIFVGTIIYPLYGNWVWGGGWLATLGSNFGLGHG
HHHCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCCC
HVDFAGSSVVHMTGGVLALVGAIIIGPRIGKYNKDGSPNAIPGHNIPMAIVGAFILAFGW
CEECCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FGFNPGSTLAGTDLRISVVAVNTMIASATGAAASMLYMWLFKTKKPDPTMMINGLLAGLV
CCCCCCCCCCCCCEEEEEEEEEHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AITAPSAFVNVQAAALIGLISGVLVIEAAFFIEKVLKIDDPVGAVAVHGVNGAWGCLALG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
LFADGTYGDGWNGVAGPVTGLFYGNPGQFYAEVIGVLANIIYVGAIGWVVFKLIDVTIGN
HHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVNPDDELDGLDIPEMGVEGYCGIRLDKNSETPLSK
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure
MVQKLIFILTGVLLMLGIAMGGDLHAYTQPDPSGAKVGVTADISAANPGKPTAAEITDQI
CHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
GKNKVSINMVWVLITGFLVMFMQAGFAMVEAGLTRAKNAAHTMSMNMMIYPIGMLGFFVS
CCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECEEEEHHHHHHHHHH
GFAIMFGGLGTLGTLGGYEGLNQEFAINLFGHTIGLFGMKGFFLNNVYDVGVFALFLFQM
HHHHHHCCCHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFMDTTATIPTGSMAERWKFSAFAIYGIFVGTIIYPLYGNWVWGGGWLATLGSNFGLGHG
HHHCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHCCCCCCCCC
HVDFAGSSVVHMTGGVLALVGAIIIGPRIGKYNKDGSPNAIPGHNIPMAIVGAFILAFGW
CEECCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FGFNPGSTLAGTDLRISVVAVNTMIASATGAAASMLYMWLFKTKKPDPTMMINGLLAGLV
CCCCCCCCCCCCCEEEEEEEEEHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AITAPSAFVNVQAAALIGLISGVLVIEAAFFIEKVLKIDDPVGAVAVHGVNGAWGCLALG
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
LFADGTYGDGWNGVAGPVTGLFYGNPGQFYAEVIGVLANIIYVGAIGWVVFKLIDVTIGN
HHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RVNPDDELDGLDIPEMGVEGYCGIRLDKNSETPLSK
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]