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The map label for this gene is rsuA [H]

Identifier: 21673314

GI number: 21673314

Start: 493940

End: 494683

Strand: Direct

Name: rsuA [H]

Synonym: CT0479

Alternate gene names: 21673314

Gene position: 493940-494683 (Clockwise)

Preceding gene: 21673313

Following gene: 21673315

Centisome position: 22.92

GC content: 59.68

Gene sequence:

>744_bases
ATGAAGAAACAGGTGCAGGAAGAGAAGGTCAGGATCAATAAATATCTCGCCATGTGCGGCGTGGCATCGCGCAGGGCCGC
CGACCAGCTTGTGCTCGAAGGCAAGGTGAGCGTCAACGGCCATATCGCCGACGAACCGGGGTTCAAGGTCGATCCTCGCA
ATGACGAGGTGATCGTCGATGGGCGACTCATGGCAACACCCGAGGCCAGAAAGGTTTACATTCTGTTCAACAAGCCCCGG
AACGTCATTACCACCAACCACGACGAGCGCGATCGCCAGCAGATTCTCGATTTCATCGATGTTCCCGAGCGGGTATTCCC
GGTCGGACGCCTCGACCGGAAGAGCACCGGTTTGCTGCTGCTGACTAACGACGGCACACTGGCCCACCGCCTGATGCACC
CGTCGTCACAGGTGCAGAAAGAGTACCTCGCTGCGCTCGACGCGAAGTTTCCGCCTGCGATGCTCCAGAAGCTGACCGGT
GGCATGAGGCTCAAGGACACCGGCGAGAAGGTGAGTCCCTGCAGAGCCAAAATCCTCGATGATGGCATGAGCGTGCTCGT
GAGCATTCACGAGGGCAAGAACCACCAGGTGCGGCGCATGTTCAGCACGCTCGGCTTCGAGGTCAAGCGCCTCGATCGCG
TGGCCTACGCGGGCCTGACCCTCGGAGAACTCCGGCGCGGCGAGTGGCGCTTTCTCTCTCGCAATGAGGTGGAAAAACTC
TATCGTTTGTGTGGCGGTCACTGA

Upstream 100 bases:

>100_bases
AGATTCTGCAGGAGCACGAGGAGCAGCAATACCTGGCTGCTGACGGCGATTTGCCGGTGGCCGCCGATGAAGATGAAAAA
CCACGAATGGAGAGGATTGA

Downstream 100 bases:

>100_bases
TTATATTTAACAAGTCCCGTTCGACGTAACGGGCTTATCGCTTTTTTTCTCTCCAATTTTCATCGACCCCTTGCGATGAA
ACCGACATACACTACCTGGC

Product: ribosomal small subunit pseudouridine synthase A

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKKQVQEEKVRINKYLAMCGVASRRAADQLVLEGKVSVNGHIADEPGFKVDPRNDEVIVDGRLMATPEARKVYILFNKPR
NVITTNHDERDRQQILDFIDVPERVFPVGRLDRKSTGLLLLTNDGTLAHRLMHPSSQVQKEYLAALDAKFPPAMLQKLTG
GMRLKDTGEKVSPCRAKILDDGMSVLVSIHEGKNHQVRRMFSTLGFEVKRLDRVAYAGLTLGELRRGEWRFLSRNEVEKL
YRLCGGH

Sequences:

>Translated_247_residues
MKKQVQEEKVRINKYLAMCGVASRRAADQLVLEGKVSVNGHIADEPGFKVDPRNDEVIVDGRLMATPEARKVYILFNKPR
NVITTNHDERDRQQILDFIDVPERVFPVGRLDRKSTGLLLLTNDGTLAHRLMHPSSQVQKEYLAALDAKFPPAMLQKLTG
GMRLKDTGEKVSPCRAKILDDGMSVLVSIHEGKNHQVRRMFSTLGFEVKRLDRVAYAGLTLGELRRGEWRFLSRNEVEKL
YRLCGGH
>Mature_247_residues
MKKQVQEEKVRINKYLAMCGVASRRAADQLVLEGKVSVNGHIADEPGFKVDPRNDEVIVDGRLMATPEARKVYILFNKPR
NVITTNHDERDRQQILDFIDVPERVFPVGRLDRKSTGLLLLTNDGTLAHRLMHPSSQVQKEYLAALDAKFPPAMLQKLTG
GMRLKDTGEKVSPCRAKILDDGMSVLVSIHEGKNHQVRRMFSTLGFEVKRLDRVAYAGLTLGELRRGEWRFLSRNEVEKL
YRLCGGH

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=34.3096234309623, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.7692307692308, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787524, Length=240, Percent_Identity=33.3333333333333, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=34.8571428571429, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 28031; Mature: 28031

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQVQEEKVRINKYLAMCGVASRRAADQLVLEGKVSVNGHIADEPGFKVDPRNDEVIVD
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEEECEEEECCEECCCCCCEECCCCCCEEEC
GRLMATPEARKVYILFNKPRNVITTNHDERDRQQILDFIDVPERVFPVGRLDRKSTGLLL
CEEEECCCCCEEEEEEECCCCCEECCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEE
LTNDGTLAHRLMHPSSQVQKEYLAALDAKFPPAMLQKLTGGMRLKDTGEKVSPCRAKILD
EECCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHH
DGMSVLVSIHEGKNHQVRRMFSTLGFEVKRLDRVAYAGLTLGELRRGEWRFLSRNEVEKL
CCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHCCCCEEECCHHHHHHH
YRLCGGH
HHHHCCC
>Mature Secondary Structure
MKKQVQEEKVRINKYLAMCGVASRRAADQLVLEGKVSVNGHIADEPGFKVDPRNDEVIVD
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHEEEECEEEECCEECCCCCCEECCCCCCEEEC
GRLMATPEARKVYILFNKPRNVITTNHDERDRQQILDFIDVPERVFPVGRLDRKSTGLLL
CEEEECCCCCEEEEEEECCCCCEECCCCHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEE
LTNDGTLAHRLMHPSSQVQKEYLAALDAKFPPAMLQKLTGGMRLKDTGEKVSPCRAKILD
EECCCHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEECCCCCCCCHHHHHHHH
DGMSVLVSIHEGKNHQVRRMFSTLGFEVKRLDRVAYAGLTLGELRRGEWRFLSRNEVEKL
CCCEEEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHCCCCEEECCHHHHHHH
YRLCGGH
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]