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The map label for this gene is fabI [H]

Identifier: 21673189

GI number: 21673189

Start: 365181

End: 366062

Strand: Direct

Name: fabI [H]

Synonym: CT0350

Alternate gene names: 21673189

Gene position: 365181-366062 (Clockwise)

Preceding gene: 21673188

Following gene: 21673190

Centisome position: 16.95

GC content: 53.74

Gene sequence:

>882_bases
ATGCCCGAGAAAGCGCACTATGGTCTTTTGAAAGGGAAAAAAGGAATTGTTTTTGGCCCGCTCGATGAAAGCAGCATCGG
TTGGCAGATTGCACTTCATGCTTACCGGGAAGGCGCACAGGTCGCCCTTTCCAATGTCGCGACAGCCATTCGCTTCGGCA
AGCTGCAGGAGCTGTCCGAGCTTTGCGGCAATGCACCGATTCTGATCTGCGATGCGTCCAAGAACGAAGAGGTAGATAAT
ACTTTCAGGGAACTCAAGGAGACCATGGGTTCTGTCGATTTTATTGTGCATTCGATCGGCATGTCCCAGAATATTCGCAA
ACAGGTGCCCTACGAGGAACTGAACTACGAGTGGTTCATGCGGACGCTCGATGTTTCCGGCATTTCGTTCCACCGGCTCG
TGGCCTATGCGCTGAAGAACGAGGCGCTCAATGACGGCGCAAGCATTGTCGCGCTTTCATACATCGCGTCGCAGCGCAAC
TACTGGACCTATTCGGACATGGGCGACGCTAAGTCGCTGCTCGAATCGATTGCACGCAGCTTCGGCCCGAGACTGGCACC
CCGTGGCATCCGGATCAACACGATTTCACAGAGCCCGACCTACACGAAAGCCGGCAGCGGTATTCCCGGTTTCGAGAAGA
TGTATGATTACAGTGAACTGATGTCGCCGCTCGGTAACGCGAGTGCTGAAGAGTGCGCCGAGTACACCATGACGATCCTG
AGCGATCTGACGCGCAAGGTGACCATGCAGAACCTTTTCCATGATGGCGGTTACAGCTCGATGGGTGCAACCATTCCTAT
GATCAAGCTTGCTCATGAGGCTCTTCATGACAAGGAGCTTGCCGAAAGAGTTGGACTCGAAGGCCGTCATTCATCAAGGT
AA

Upstream 100 bases:

>100_bases
CTGTTTAACAATTCACCGGAAACTTGTATCTTGACAGCTTTGAACGATCATCAAAGGGGTATCCGCTACCCTTATTTTCT
GAATAGAATCAGATTGTATC

Downstream 100 bases:

>100_bases
GCATTGATGGAGCGCCGGCTTCCGCTGGCCTCCCGTTTTCCTTCATCACTTCCATACCCATTCGTCTTTGGCCTTGCTGA
CCAGGGCTAGAAGGTAGGTA

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPEKAHYGLLKGKKGIVFGPLDESSIGWQIALHAYREGAQVALSNVATAIRFGKLQELSELCGNAPILICDASKNEEVDN
TFRELKETMGSVDFIVHSIGMSQNIRKQVPYEELNYEWFMRTLDVSGISFHRLVAYALKNEALNDGASIVALSYIASQRN
YWTYSDMGDAKSLLESIARSFGPRLAPRGIRINTISQSPTYTKAGSGIPGFEKMYDYSELMSPLGNASAEECAEYTMTIL
SDLTRKVTMQNLFHDGGYSSMGATIPMIKLAHEALHDKELAERVGLEGRHSSR

Sequences:

>Translated_293_residues
MPEKAHYGLLKGKKGIVFGPLDESSIGWQIALHAYREGAQVALSNVATAIRFGKLQELSELCGNAPILICDASKNEEVDN
TFRELKETMGSVDFIVHSIGMSQNIRKQVPYEELNYEWFMRTLDVSGISFHRLVAYALKNEALNDGASIVALSYIASQRN
YWTYSDMGDAKSLLESIARSFGPRLAPRGIRINTISQSPTYTKAGSGIPGFEKMYDYSELMSPLGNASAEECAEYTMTIL
SDLTRKVTMQNLFHDGGYSSMGATIPMIKLAHEALHDKELAERVGLEGRHSSR
>Mature_292_residues
PEKAHYGLLKGKKGIVFGPLDESSIGWQIALHAYREGAQVALSNVATAIRFGKLQELSELCGNAPILICDASKNEEVDNT
FRELKETMGSVDFIVHSIGMSQNIRKQVPYEELNYEWFMRTLDVSGISFHRLVAYALKNEALNDGASIVALSYIASQRNY
WTYSDMGDAKSLLESIARSFGPRLAPRGIRINTISQSPTYTKAGSGIPGFEKMYDYSELMSPLGNASAEECAEYTMTILS
DLTRKVTMQNLFHDGGYSSMGATIPMIKLAHEALHDKELAERVGLEGRHSSR

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=254, Percent_Identity=31.496062992126, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 32445; Mature: 32314

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKAHYGLLKGKKGIVFGPLDESSIGWQIALHAYREGAQVALSNVATAIRFGKLQELSE
CCCCCCCCHHCCCCCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LCGNAPILICDASKNEEVDNTFRELKETMGSVDFIVHSIGMSQNIRKQVPYEELNYEWFM
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCHHHHH
RTLDVSGISFHRLVAYALKNEALNDGASIVALSYIASQRNYWTYSDMGDAKSLLESIARS
HHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FGPRLAPRGIRINTISQSPTYTKAGSGIPGFEKMYDYSELMSPLGNASAEECAEYTMTIL
HCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SDLTRKVTMQNLFHDGGYSSMGATIPMIKLAHEALHDKELAERVGLEGRHSSR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PEKAHYGLLKGKKGIVFGPLDESSIGWQIALHAYREGAQVALSNVATAIRFGKLQELSE
CCCCCCCHHCCCCCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LCGNAPILICDASKNEEVDNTFRELKETMGSVDFIVHSIGMSQNIRKQVPYEELNYEWFM
HHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCHHHHH
RTLDVSGISFHRLVAYALKNEALNDGASIVALSYIASQRNYWTYSDMGDAKSLLESIARS
HHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
FGPRLAPRGIRINTISQSPTYTKAGSGIPGFEKMYDYSELMSPLGNASAEECAEYTMTIL
HCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SDLTRKVTMQNLFHDGGYSSMGATIPMIKLAHEALHDKELAERVGLEGRHSSR
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]