Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 21673158

GI number: 21673158

Start: 334852

End: 335451

Strand: Reverse

Name: purN [H]

Synonym: CT0319

Alternate gene names: 21673158

Gene position: 335451-334852 (Counterclockwise)

Preceding gene: 21673160

Following gene: 21673157

Centisome position: 15.57

GC content: 60.0

Gene sequence:

>600_bases
ATGATTGACAAGAAAAAACGGTTGGCGGTATTCTGCTCGGGAACGGGCTCAAACTTCAAGGCGCTGTTCCACGCGATCAT
CGAACGCGAGCTGCCTGCCGAGATCGTGATGTGCCTGTCGAATCGCTCGCAGTGCGGCGCGATCGATTTCGCAAAAGAGT
ATGGCATCGAGACACTGCACCTCTCGGAATCGCAGTTCGGCTCGCATGACGATTTCGCCCGCGCCATGCTGAGCGAACTC
CGGGATCGGCAGATCGACATGATCCTGCTCGCCGGGTATCTGCGCAAGATTCCGGACGCGGTGATTGCCGCCTACCCGGA
AAAAATCGTGAACATCCATCCGTCGCTGCTGCCGCAGTTCGGCGGCCATGGCATGTACGGAATGCGCGTGCACGAGGCGG
TGATCGCGTCGGGTGAAACCCGGAGCGGGGCGACGGTTCACTTCGTCAACGAGGAGTACGACAAGGGCCGGATCATCATG
CAGAATCATGTGCCGGTGCTGCCAGGCGACACGCCAAAAACACTCGCCGAGCGGGTGCTCCGGTGCGAGCATCGCCTCTA
CCCCGCCGCGCTCGAAAAACTGCTTGACAAGCAGCCATGA

Upstream 100 bases:

>100_bases
CTCTTAACTGAACCGTTCGCGGGGAACCTTTCAGCTTGAAAAAAGCAACGTTCGGGTGCATATTAGAGGGAATTCACGAA
ATCCTTTGTACGCGATCTGC

Downstream 100 bases:

>100_bases
GCACGGAAGCCCCCCTCAATATCAGCGAGATTTTTTACTCAATCCAGGGTGAGTCTTCGTTTGCAGGCTGGCCTTGCGCC
TTCGTGCGCCTCGCCGGATG

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIDKKKRLAVFCSGTGSNFKALFHAIIERELPAEIVMCLSNRSQCGAIDFAKEYGIETLHLSESQFGSHDDFARAMLSEL
RDRQIDMILLAGYLRKIPDAVIAAYPEKIVNIHPSLLPQFGGHGMYGMRVHEAVIASGETRSGATVHFVNEEYDKGRIIM
QNHVPVLPGDTPKTLAERVLRCEHRLYPAALEKLLDKQP

Sequences:

>Translated_199_residues
MIDKKKRLAVFCSGTGSNFKALFHAIIERELPAEIVMCLSNRSQCGAIDFAKEYGIETLHLSESQFGSHDDFARAMLSEL
RDRQIDMILLAGYLRKIPDAVIAAYPEKIVNIHPSLLPQFGGHGMYGMRVHEAVIASGETRSGATVHFVNEEYDKGRIIM
QNHVPVLPGDTPKTLAERVLRCEHRLYPAALEKLLDKQP
>Mature_199_residues
MIDKKKRLAVFCSGTGSNFKALFHAIIERELPAEIVMCLSNRSQCGAIDFAKEYGIETLHLSESQFGSHDDFARAMLSEL
RDRQIDMILLAGYLRKIPDAVIAAYPEKIVNIHPSLLPQFGGHGMYGMRVHEAVIASGETRSGATVHFVNEEYDKGRIIM
QNHVPVLPGDTPKTLAERVLRCEHRLYPAALEKLLDKQP

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=189, Percent_Identity=40.2116402116402, Blast_Score=141, Evalue=4e-34,
Organism=Homo sapiens, GI209869995, Length=189, Percent_Identity=40.2116402116402, Blast_Score=141, Evalue=4e-34,
Organism=Homo sapiens, GI209869993, Length=189, Percent_Identity=40.2116402116402, Blast_Score=141, Evalue=4e-34,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=31.5789473684211, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787483, Length=115, Percent_Identity=33.9130434782609, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17567511, Length=204, Percent_Identity=31.8627450980392, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582400, Length=195, Percent_Identity=35.8974358974359, Blast_Score=134, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22272; Mature: 22272

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDKKKRLAVFCSGTGSNFKALFHAIIERELPAEIVMCLSNRSQCGAIDFAKEYGIETLH
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHCCEEEE
LSESQFGSHDDFARAMLSELRDRQIDMILLAGYLRKIPDAVIAAYPEKIVNIHPSLLPQF
ECHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHC
GGHGMYGMRVHEAVIASGETRSGATVHFVNEEYDKGRIIMQNHVPVLPGDTPKTLAERVL
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHH
RCEHRLYPAALEKLLDKQP
HHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIDKKKRLAVFCSGTGSNFKALFHAIIERELPAEIVMCLSNRSQCGAIDFAKEYGIETLH
CCCCCCEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHCCEEEE
LSESQFGSHDDFARAMLSELRDRQIDMILLAGYLRKIPDAVIAAYPEKIVNIHPSLLPQF
ECHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHC
GGHGMYGMRVHEAVIASGETRSGATVHFVNEEYDKGRIIMQNHVPVLPGDTPKTLAERVL
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHH
RCEHRLYPAALEKLLDKQP
HHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]