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The map label for this gene is rfbA [H]

Identifier: 21673144

GI number: 21673144

Start: 319380

End: 320261

Strand: Direct

Name: rfbA [H]

Synonym: CT0305

Alternate gene names: 21673144

Gene position: 319380-320261 (Clockwise)

Preceding gene: 21673142

Following gene: 21673145

Centisome position: 14.82

GC content: 54.65

Gene sequence:

>882_bases
ATGAAGGGTATCATTCTTGCCGGAGGGTCAGGCACCCGTCTGTATCCTGTGACCAAGGGCGTATCGAAGCAGTTGCTTCC
GGTGTACGACAAGCCGATGATCTACTATCCGCTCACCACCCTGATGCTTGCCGGTATCAGGGATATTCTCGTCATTACCA
CTCCCGATGATCAGTCTTCGTTCGTCAAGCTGCTCGGTGATGGAAGCGACTGGGGCATCAACCTTTCCTATACGGTTCAA
CCCTCGCCCGACGGACTGGCGCAGGCTTTCATTCTCGGTCGCGATTTTATTGGCGATGACGATGTCTGTCTCGTGCTTGG
CGACAACATCTTTTTTGGTTATGGTTTCAGCGGGATGCTCGAAGAGGCGGTTCATGTGGTCGAGAGACGGAGAAAAGCGG
TGGTTTTCGGCTACTATGTCAGTGATCCTGAGCGTTACGGTGTAGTCGAGTTCGATTCGGACGGGCAGGTGTTCTCCATA
GTTGAGAAGCCGGAAAAACCGAAATCGAACTACGCGGTCGTGGGGCTCTATTTCTATCCGAACGATGTGATTGATATTGC
CGCCAGCGTCAATCCGTCGTCGAGAGGGGAACTCGAGATCACCTCGGTGAACCAGACGTACCTCGATCGCGGCGATCTGG
TTTGTTCCATCATGGGGCGTGGATTTGCCTGGCTCGATACCGGCACGCACGAATCATTCCAGGAGGCAGGCAACTTCATT
GAAACCGTCGAGAAGCGGCAGGGGCTCAAGGTAGCCTGCCCAGAAGAGATTGCCTGGCGGAACGGCTGGATCGGCGATGC
CGACATCGAACGTCTCGCCTCGCCGCTCCTCAAGAACCAGTATGGGCAGTATCTGCTCAATCTTCTGGAACGGAGAATCT
GA

Upstream 100 bases:

>100_bases
ATTTTAAAAAAATGTATATATAGTAGATTTAAAGGCTAAGTCGCTTGAGAAGCTGCGTCGTGGAAACATGACCATCGTTG
ACCGAAGACCAACAACCACC

Downstream 100 bases:

>100_bases
ACATCAATCCATCAAGTAGTACTGTAACCTATGCAGATCATCCGGACCTCAATTCCTGACGTTCTTCTGTTCGAACCAGA
GGTGTTCGGCGATGAACGCG

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKGIILAGGSGTRLYPVTKGVSKQLLPVYDKPMIYYPLTTLMLAGIRDILVITTPDDQSSFVKLLGDGSDWGINLSYTVQ
PSPDGLAQAFILGRDFIGDDDVCLVLGDNIFFGYGFSGMLEEAVHVVERRRKAVVFGYYVSDPERYGVVEFDSDGQVFSI
VEKPEKPKSNYAVVGLYFYPNDVIDIAASVNPSSRGELEITSVNQTYLDRGDLVCSIMGRGFAWLDTGTHESFQEAGNFI
ETVEKRQGLKVACPEEIAWRNGWIGDADIERLASPLLKNQYGQYLLNLLERRI

Sequences:

>Translated_293_residues
MKGIILAGGSGTRLYPVTKGVSKQLLPVYDKPMIYYPLTTLMLAGIRDILVITTPDDQSSFVKLLGDGSDWGINLSYTVQ
PSPDGLAQAFILGRDFIGDDDVCLVLGDNIFFGYGFSGMLEEAVHVVERRRKAVVFGYYVSDPERYGVVEFDSDGQVFSI
VEKPEKPKSNYAVVGLYFYPNDVIDIAASVNPSSRGELEITSVNQTYLDRGDLVCSIMGRGFAWLDTGTHESFQEAGNFI
ETVEKRQGLKVACPEEIAWRNGWIGDADIERLASPLLKNQYGQYLLNLLERRI
>Mature_293_residues
MKGIILAGGSGTRLYPVTKGVSKQLLPVYDKPMIYYPLTTLMLAGIRDILVITTPDDQSSFVKLLGDGSDWGINLSYTVQ
PSPDGLAQAFILGRDFIGDDDVCLVLGDNIFFGYGFSGMLEEAVHVVERRRKAVVFGYYVSDPERYGVVEFDSDGQVFSI
VEKPEKPKSNYAVVGLYFYPNDVIDIAASVNPSSRGELEITSVNQTYLDRGDLVCSIMGRGFAWLDTGTHESFQEAGNFI
ETVEKRQGLKVACPEEIAWRNGWIGDADIERLASPLLKNQYGQYLLNLLERRI

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=277, Percent_Identity=25.2707581227437, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI11761619, Length=277, Percent_Identity=25.2707581227437, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=65.8536585365854, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1790224, Length=292, Percent_Identity=65.4109589041096, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI133931050, Length=238, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320148, Length=278, Percent_Identity=28.4172661870504, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355443, Length=234, Percent_Identity=26.4957264957265, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24644084, Length=234, Percent_Identity=26.4957264957265, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32521; Mature: 32521

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGSGTRLYPVTKGVSKQLLPVYDKPMIYYPLTTLMLAGIRDILVITTPDDQSS
CCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCH
FVKLLGDGSDWGINLSYTVQPSPDGLAQAFILGRDFIGDDDVCLVLGDNIFFGYGFSGML
HHEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCHHHH
EEAVHVVERRRKAVVFGYYVSDPERYGVVEFDSDGQVFSIVEKPEKPKSNYAVVGLYFYP
HHHHHHHHHCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCEEEEEEEECC
NDVIDIAASVNPSSRGELEITSVNQTYLDRGDLVCSIMGRGFAWLDTGTHESFQEAGNFI
CCEEEEEEECCCCCCCCEEEEECCHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHH
ETVEKRQGLKVACPEEIAWRNGWIGDADIERLASPLLKNQYGQYLLNLLERRI
HHHHHHCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKGIILAGGSGTRLYPVTKGVSKQLLPVYDKPMIYYPLTTLMLAGIRDILVITTPDDQSS
CCCEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHCCCEEEEEECCCCCCH
FVKLLGDGSDWGINLSYTVQPSPDGLAQAFILGRDFIGDDDVCLVLGDNIFFGYGFSGML
HHEEEECCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEECCEEEECCCHHHH
EEAVHVVERRRKAVVFGYYVSDPERYGVVEFDSDGQVFSIVEKPEKPKSNYAVVGLYFYP
HHHHHHHHHCCCEEEEEEEECCCCCCCEEEECCCCCEEEEECCCCCCCCCEEEEEEEECC
NDVIDIAASVNPSSRGELEITSVNQTYLDRGDLVCSIMGRGFAWLDTGTHESFQEAGNFI
CCEEEEEEECCCCCCCCEEEEECCHHHHCCCCEEEEECCCCEEEEECCCCHHHHHHHHHH
ETVEKRQGLKVACPEEIAWRNGWIGDADIERLASPLLKNQYGQYLLNLLERRI
HHHHHHCCCEEECCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]