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The map label for this gene is yafV [C]

Identifier: 21673113

GI number: 21673113

Start: 286190

End: 287005

Strand: Direct

Name: yafV [C]

Synonym: CT0274

Alternate gene names: 21673113

Gene position: 286190-287005 (Clockwise)

Preceding gene: 21673112

Following gene: 21673119

Centisome position: 13.28

GC content: 61.52

Gene sequence:

>816_bases
ATGATCCGTCTCGCCACCGTCCAGTTTACGCCACGTCTCGGGGAGCGCCAGGCGAATCTCGAAGCGATCCGGAGTTTGCT
CGATCCGGTCGAAGCGGACATTGTCGTGCTGCCGGAGCTGTGCTCCAGCGGCTACTTTTTCACGTCGCGTGAAGAGCTTG
CGCCGTTTGCCGAATCGCCGGGCGGCGTGGCGTGCAGTTTTTTCCAGGGCCTCGCCGACGCGAAGCGTGCGATCATCATC
GCCGGAATGCCCGAAACGGCGCAAGGTTGCTTCTACAATTCGGTGTTCGTGTTCCGGCCCGGCGTTGCTGACCCGCTCGT
TTACCGCAAGTCTCATCTTTTCTACAAGGAGCGCTTCGTGTTTGAGCCGGGCGACACCGGCTTTCCGGTGATCCGCGACG
AGCAGCTCGACATTTCAATCGGCATCATGCTTTGCTACGACTGGCGTTTTCCGGAGGTGTCGCGGGTGCTGGCCCTCGGC
GGGGCCGACCTCATTGCCTGCCCGTCCAACCTTGTCACCGATGCATGGCGCAAGGTGATGCCTGCTCGCGCTATCGAGAA
CAAGCTGTACGTCGCCGTGGCCAATCGGTGCGGTACTGAAACGAGGGGTGATGAGACGCTGCTGTTCAAGGGGTGTTCGG
CGGTGTACGATCCTTACGGAGAGACGGTCGCACTTGCCGACGCTGACAACGACCGGGTGCTGCTGGCGGAGATCGATCCA
CGATCATGCCGCGACAAGAGCTTCAACGAGTTCAACGATATTTTCGCCGACCGGCGGCCCGAGCTGTACGGCGCGATCTG
CTGTCCCCGCCGCTGA

Upstream 100 bases:

>100_bases
TCTATCTCGTGCTCGGCACGCTCATTTTCCGCAACGAGGCGTCGGCTGTCGTGAAGATGGTGCAGAGAAAATTGCGTCCG
GCGGGAAGTTCCGGCAGCCG

Downstream 100 bases:

>100_bases
AGTCCTCGATCTCCGCTTACCGTTTTTCAGCCATCCGGTTGATCCTGCTGACCAGTTCGGAGGGCTCCATGCTGTTTTTC
GTGGCGATGTCGCGAATCGA

Product: carbon-nitrogen hydrolase family protein

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MIRLATVQFTPRLGERQANLEAIRSLLDPVEADIVVLPELCSSGYFFTSREELAPFAESPGGVACSFFQGLADAKRAIII
AGMPETAQGCFYNSVFVFRPGVADPLVYRKSHLFYKERFVFEPGDTGFPVIRDEQLDISIGIMLCYDWRFPEVSRVLALG
GADLIACPSNLVTDAWRKVMPARAIENKLYVAVANRCGTETRGDETLLFKGCSAVYDPYGETVALADADNDRVLLAEIDP
RSCRDKSFNEFNDIFADRRPELYGAICCPRR

Sequences:

>Translated_271_residues
MIRLATVQFTPRLGERQANLEAIRSLLDPVEADIVVLPELCSSGYFFTSREELAPFAESPGGVACSFFQGLADAKRAIII
AGMPETAQGCFYNSVFVFRPGVADPLVYRKSHLFYKERFVFEPGDTGFPVIRDEQLDISIGIMLCYDWRFPEVSRVLALG
GADLIACPSNLVTDAWRKVMPARAIENKLYVAVANRCGTETRGDETLLFKGCSAVYDPYGETVALADADNDRVLLAEIDP
RSCRDKSFNEFNDIFADRRPELYGAICCPRR
>Mature_271_residues
MIRLATVQFTPRLGERQANLEAIRSLLDPVEADIVVLPELCSSGYFFTSREELAPFAESPGGVACSFFQGLADAKRAIII
AGMPETAQGCFYNSVFVFRPGVADPLVYRKSHLFYKERFVFEPGDTGFPVIRDEQLDISIGIMLCYDWRFPEVSRVLALG
GADLIACPSNLVTDAWRKVMPARAIENKLYVAVANRCGTETRGDETLLFKGCSAVYDPYGETVALADADNDRVLLAEIDP
RSCRDKSFNEFNDIFADRRPELYGAICCPRR

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=248, Percent_Identity=29.0322580645161, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI297632348, Length=134, Percent_Identity=37.3134328358209, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI5031947, Length=134, Percent_Identity=37.3134328358209, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI297632350, Length=134, Percent_Identity=37.3134328358209, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1786412, Length=236, Percent_Identity=30.0847457627119, Blast_Score=71, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323383, Length=287, Percent_Identity=27.1777003484321, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30179; Mature: 30179

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50263 CN_HYDROLASE ; PS00539 PYROKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLATVQFTPRLGERQANLEAIRSLLDPVEADIVVLPELCSSGYFFTSREELAPFAESP
CEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHCCCCEEECHHHHCCCCCCC
GGVACSFFQGLADAKRAIIIAGMPETAQGCFYNSVFVFRPGVADPLVYRKSHLFYKERFV
CCEEHHHHHHHHCCCEEEEEECCCCHHCCCEECEEEEECCCCCCCHHHHHHHHEEEEEEE
FEPGDTGFPVIRDEQLDISIGIMLCYDWRFPEVSRVLALGGADLIACPSNLVTDAWRKVM
ECCCCCCCCEEECCCCEEEEEEEEEEECCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHH
PARAIENKLYVAVANRCGTETRGDETLLFKGCSAVYDPYGETVALADADNDRVLLAEIDP
CHHHHCCEEEEEEECCCCCCCCCCCEEEEECCHHHHCCCCCEEEEEECCCCEEEEEECCC
RSCRDKSFNEFNDIFADRRPELYGAICCPRR
CCCCCCCHHHHHHHHHCCCCCEEEEECCCCC
>Mature Secondary Structure
MIRLATVQFTPRLGERQANLEAIRSLLDPVEADIVVLPELCSSGYFFTSREELAPFAESP
CEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEEHHHCCCCEEECHHHHCCCCCCC
GGVACSFFQGLADAKRAIIIAGMPETAQGCFYNSVFVFRPGVADPLVYRKSHLFYKERFV
CCEEHHHHHHHHCCCEEEEEECCCCHHCCCEECEEEEECCCCCCCHHHHHHHHEEEEEEE
FEPGDTGFPVIRDEQLDISIGIMLCYDWRFPEVSRVLALGGADLIACPSNLVTDAWRKVM
ECCCCCCCCEEECCCCEEEEEEEEEEECCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHH
PARAIENKLYVAVANRCGTETRGDETLLFKGCSAVYDPYGETVALADADNDRVLLAEIDP
CHHHHCCEEEEEEECCCCCCCCCCCEEEEECCHHHHCCCCCEEEEEECCCCEEEEEECCC
RSCRDKSFNEFNDIFADRRPELYGAICCPRR
CCCCCCCHHHHHHHHHCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8526497 [H]