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The map label for this gene is gatA [H]

Identifier: 21673107

GI number: 21673107

Start: 280610

End: 282055

Strand: Direct

Name: gatA [H]

Synonym: CT0268

Alternate gene names: 21673107

Gene position: 280610-282055 (Clockwise)

Preceding gene: 21673104

Following gene: 21673108

Centisome position: 13.02

GC content: 59.96

Gene sequence:

>1446_bases
GTGCAGTTTCATGGTTACGAGGACCTCAGGTCCAGACTGCTCTCAGGTGAGCTAACCTGTGAGCAGGTGATTTCAGATTA
TTTACAGCGCATCGATTCCAGCCGCGACGACAATATTTTCACCGTCGTGTTTCATGACGAGGCGATGGCTCGCGCCCGCG
AGCTTGACAGCAAGCTTCAGCGCGGCGAAGCGCCCGGGGTGCTTTTCGGTATGCCGATTGCCATCAAGGACAACATTGCC
ATGAAGGGGGCGCCTCTGTCGTGTGCCTCGAAGATTCTCGCTGGTTACGAAAGCGTCTATGACGCTACGGTGATCAAGCG
GATGCAGGCCGAGGATGCCATTTTTGTTGGACGCACCAACATGGACGAGTTTGCGATGGGCAGTTCCAACGAGAACTCCG
CCATCGGCCCGGTACCCAACCCCTACGATAAAACCCGCGTGCCCGGTGGCAGCTCCGGCGGCTCGGCGGCAGCGGTGGCT
AACGATCTCGCCATGGTGGCGCTTGGCTCCGATACCGGCGGTTCGGTTCGCCAGCCTGCAGGTTTCTGCAACATTATTGG
TCTCAAGCCAACCTATGGCCGTATCTCGCGTTACGGCCTCGTCGCCTTCGCTTCGTCATTCGACCAGATCGGCCTGCTCG
CCGCCAACTGCGACGACGCAGCGCTCGTGCTTGGTGTTATTGCCGGAAAGGACGAGCATGATGCAACCTCGTCGCACCAC
GACGTGCCGGAGTACGATACCGCGATGGATCACGTCTCCGTCGATGGGCTGCGCATCGGCGTGCCGCGCGCATTTTTCCC
CGAGAGCCTCAATGCCGACGTGGCCGGAGTGGTCAAGGCAGGGCTGAAAAAGCTCGAAGAAGCGGGCGCGGAGCTGGTCG
AGATCGACCTGCCGGAGAGCGATTACGCCATCGCGGCCTATTACATTCTTGTCACCGCCGAAGCCTCCTCGAACCTCGCA
CGGTTCGACGGCGCACGCTACGGCTACCGTTCTCCCGATTCGCCCGACCTTTCGAGCATGTACGTGAACTCGCGCACCGA
GGGATTCGGCGCGGAGGTCAAGCGTCGCATCATGCTTGGCACCTACGTGCTTTCGGCGGGCTATTACGACACCTACTACA
AAAAAGCGCAGCAGGTACGCCGCGTCTTCCAGGACAAGTATCGCGAAGCGTTCGAAAAGGTCGATGTGATCTTCGGGCCG
ACCTCGCCCTTTCCACCCTTCGGCATCGGCGACAAGATGGACAATCCGCTCGAAATGTACCTGGCCGACGTCTTCACCGT
CCCGGCCAGCATTGTCGGGATGCCCGCCATCAGCGTGCCGGTCGGCTTTGACAGCCTTGGCCTGCCGGTTGGCGCCCACC
TGATCTGCAACTTTTTCGAGGAGGGCAAGATGCTTGGCATTGCACGCCATCTCCAGACCTTGTGTCAGACAGCTCCGTCC
AACTGA

Upstream 100 bases:

>100_bases
TGGACTCTTCCGGCCGGCAGCGCCCTCTATCCGCAGGAGTTGTTGTCCCGCCGCAACATGCAGATGAACGATCTTCAATT
TATAATCTAGCCGAATAAAC

Downstream 100 bases:

>100_bases
CGGTTTTTTTTCATCACCAAAACGAATATCGGCATATGAGCGTACTGGTCAATAAAGATACCCGCCTGGTCGTCCAGGGA
ATTACTGGCGGCGAGGGAAC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MQFHGYEDLRSRLLSGELTCEQVISDYLQRIDSSRDDNIFTVVFHDEAMARARELDSKLQRGEAPGVLFGMPIAIKDNIA
MKGAPLSCASKILAGYESVYDATVIKRMQAEDAIFVGRTNMDEFAMGSSNENSAIGPVPNPYDKTRVPGGSSGGSAAAVA
NDLAMVALGSDTGGSVRQPAGFCNIIGLKPTYGRISRYGLVAFASSFDQIGLLAANCDDAALVLGVIAGKDEHDATSSHH
DVPEYDTAMDHVSVDGLRIGVPRAFFPESLNADVAGVVKAGLKKLEEAGAELVEIDLPESDYAIAAYYILVTAEASSNLA
RFDGARYGYRSPDSPDLSSMYVNSRTEGFGAEVKRRIMLGTYVLSAGYYDTYYKKAQQVRRVFQDKYREAFEKVDVIFGP
TSPFPPFGIGDKMDNPLEMYLADVFTVPASIVGMPAISVPVGFDSLGLPVGAHLICNFFEEGKMLGIARHLQTLCQTAPS
N

Sequences:

>Translated_481_residues
MQFHGYEDLRSRLLSGELTCEQVISDYLQRIDSSRDDNIFTVVFHDEAMARARELDSKLQRGEAPGVLFGMPIAIKDNIA
MKGAPLSCASKILAGYESVYDATVIKRMQAEDAIFVGRTNMDEFAMGSSNENSAIGPVPNPYDKTRVPGGSSGGSAAAVA
NDLAMVALGSDTGGSVRQPAGFCNIIGLKPTYGRISRYGLVAFASSFDQIGLLAANCDDAALVLGVIAGKDEHDATSSHH
DVPEYDTAMDHVSVDGLRIGVPRAFFPESLNADVAGVVKAGLKKLEEAGAELVEIDLPESDYAIAAYYILVTAEASSNLA
RFDGARYGYRSPDSPDLSSMYVNSRTEGFGAEVKRRIMLGTYVLSAGYYDTYYKKAQQVRRVFQDKYREAFEKVDVIFGP
TSPFPPFGIGDKMDNPLEMYLADVFTVPASIVGMPAISVPVGFDSLGLPVGAHLICNFFEEGKMLGIARHLQTLCQTAPS
N
>Mature_481_residues
MQFHGYEDLRSRLLSGELTCEQVISDYLQRIDSSRDDNIFTVVFHDEAMARARELDSKLQRGEAPGVLFGMPIAIKDNIA
MKGAPLSCASKILAGYESVYDATVIKRMQAEDAIFVGRTNMDEFAMGSSNENSAIGPVPNPYDKTRVPGGSSGGSAAAVA
NDLAMVALGSDTGGSVRQPAGFCNIIGLKPTYGRISRYGLVAFASSFDQIGLLAANCDDAALVLGVIAGKDEHDATSSHH
DVPEYDTAMDHVSVDGLRIGVPRAFFPESLNADVAGVVKAGLKKLEEAGAELVEIDLPESDYAIAAYYILVTAEASSNLA
RFDGARYGYRSPDSPDLSSMYVNSRTEGFGAEVKRRIMLGTYVLSAGYYDTYYKKAQQVRRVFQDKYREAFEKVDVIFGP
TSPFPPFGIGDKMDNPLEMYLADVFTVPASIVGMPAISVPVGFDSLGLPVGAHLICNFFEEGKMLGIARHLQTLCQTAPS
N

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=492, Percent_Identity=33.739837398374, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI195972892, Length=477, Percent_Identity=24.9475890985325, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI166795287, Length=191, Percent_Identity=29.3193717277487, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=476, Percent_Identity=34.8739495798319, Blast_Score=264, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=489, Percent_Identity=25.3578732106339, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=310, Percent_Identity=28.0645161290323, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=196, Percent_Identity=30.1020408163265, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=321, Percent_Identity=25.5451713395639, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=298, Percent_Identity=27.5167785234899, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=298, Percent_Identity=27.5167785234899, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=197, Percent_Identity=27.9187817258883, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=419, Percent_Identity=34.8448687350835, Blast_Score=227, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319685, Length=448, Percent_Identity=29.4642857142857, Blast_Score=133, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24648113, Length=476, Percent_Identity=35.2941176470588, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=24.9496981891348, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=24.9496981891348, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=24.9496981891348, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=24.9496981891348, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21356731, Length=472, Percent_Identity=25.635593220339, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550774, Length=194, Percent_Identity=31.4432989690722, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648435, Length=194, Percent_Identity=31.4432989690722, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24648437, Length=194, Percent_Identity=31.4432989690722, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24644968, Length=496, Percent_Identity=23.3870967741935, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI161078093, Length=472, Percent_Identity=22.4576271186441, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648441, Length=124, Percent_Identity=37.0967741935484, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=124, Percent_Identity=37.0967741935484, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51858; Mature: 51858

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFHGYEDLRSRLLSGELTCEQVISDYLQRIDSSRDDNIFTVVFHDEAMARARELDSKLQ
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHH
RGEAPGVLFGMPIAIKDNIAMKGAPLSCASKILAGYESVYDATVIKRMQAEDAIFVGRTN
CCCCCCEEEECCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
MDEFAMGSSNENSAIGPVPNPYDKTRVPGGSSGGSAAAVANDLAMVALGSDTGGSVRQPA
CHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCC
GFCNIIGLKPTYGRISRYGLVAFASSFDQIGLLAANCDDAALVLGVIAGKDEHDATSSHH
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCC
DVPEYDTAMDHVSVDGLRIGVPRAFFPESLNADVAGVVKAGLKKLEEAGAELVEIDLPES
CCCCHHHHHHHEECCCEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
DYAIAAYYILVTAEASSNLARFDGARYGYRSPDSPDLSSMYVNSRTEGFGAEVKRRIMLG
CCEEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHH
TYVLSAGYYDTYYKKAQQVRRVFQDKYREAFEKVDVIFGPTSPFPPFGIGDKMDNPLEMY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHH
LADVFTVPASIVGMPAISVPVGFDSLGLPVGAHLICNFFEEGKMLGIARHLQTLCQTAPS
HHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
N
C
>Mature Secondary Structure
MQFHGYEDLRSRLLSGELTCEQVISDYLQRIDSSRDDNIFTVVFHDEAMARARELDSKLQ
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHH
RGEAPGVLFGMPIAIKDNIAMKGAPLSCASKILAGYESVYDATVIKRMQAEDAIFVGRTN
CCCCCCEEEECCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
MDEFAMGSSNENSAIGPVPNPYDKTRVPGGSSGGSAAAVANDLAMVALGSDTGGSVRQPA
CHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCC
GFCNIIGLKPTYGRISRYGLVAFASSFDQIGLLAANCDDAALVLGVIAGKDEHDATSSHH
CCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCC
DVPEYDTAMDHVSVDGLRIGVPRAFFPESLNADVAGVVKAGLKKLEEAGAELVEIDLPES
CCCCHHHHHHHEECCCEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
DYAIAAYYILVTAEASSNLARFDGARYGYRSPDSPDLSSMYVNSRTEGFGAEVKRRIMLG
CCEEEEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHEECCCCCCCCHHHHHHHHHH
TYVLSAGYYDTYYKKAQQVRRVFQDKYREAFEKVDVIFGPTSPFPPFGIGDKMDNPLEMY
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCHHHHH
LADVFTVPASIVGMPAISVPVGFDSLGLPVGAHLICNFFEEGKMLGIARHLQTLCQTAPS
HHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA