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The map label for this gene is hupB [H]

Identifier: 21673001

GI number: 21673001

Start: 167577

End: 167852

Strand: Direct

Name: hupB [H]

Synonym: CT0160

Alternate gene names: 21673001

Gene position: 167577-167852 (Clockwise)

Preceding gene: 21672997

Following gene: 21673002

Centisome position: 7.78

GC content: 54.35

Gene sequence:

>276_bases
ATGTCGAAAGCCGAGTTAGCAGAAAAGATTGCAGAGCAGACCGGTTTGACCAAGGCTGATGCTGAAAGAGCGGTCAATGC
ATTCATCAATGTGGTGACCTCGACCCTGAAAAGCGGTGATGATGTGACCCTGGTGGGCTTTGGTACTTTTACCACCGGCG
ACCGGGCAGAGCGCCAGGGCCGCAATCCCCAGACAGGCAAGACCATTACCATTGCCGCCAAGAAGGTTGTCAAGTTCAAG
CCTGGCAAAGCGCTGAAAGAAGAGGTCGGTGGCTGA

Upstream 100 bases:

>100_bases
AATTGGATGTTTGTTGGGCAGTCTTTAAATTGAATTATTGTAGTAATTGGAAATAGGCAACCTGATTTTCCTCAAACATT
TAAACGGTAAACTACTTATT

Downstream 100 bases:

>100_bases
GCCATTACCGGAGCTTACAAGCTCATCGAACGAGTTTTCAAGGTCCATCATTGGCATGAGCAGTGATGGACTTTTTTTTA
AGGAGCAAGCATACTATGGC

Product: DNA-binding protein HU-beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 90

Protein sequence:

>91_residues
MSKAELAEKIAEQTGLTKADAERAVNAFINVVTSTLKSGDDVTLVGFGTFTTGDRAERQGRNPQTGKTITIAAKKVVKFK
PGKALKEEVGG

Sequences:

>Translated_91_residues
MSKAELAEKIAEQTGLTKADAERAVNAFINVVTSTLKSGDDVTLVGFGTFTTGDRAERQGRNPQTGKTITIAAKKVVKFK
PGKALKEEVGG
>Mature_90_residues
SKAELAEKIAEQTGLTKADAERAVNAFINVVTSTLKSGDDVTLVGFGTFTTGDRAERQGRNPQTGKTITIAAKKVVKFKP
GKALKEEVGG

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=59.5505617977528, Blast_Score=101, Evalue=7e-24,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=52.8089887640449, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=42.6966292134831, Blast_Score=84, Evalue=3e-18,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=39.7727272727273, Blast_Score=71, Evalue=1e-14,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9595; Mature: 9464

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAELAEKIAEQTGLTKADAERAVNAFINVVTSTLKSGDDVTLVGFGTFTTGDRAERQG
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCC
RNPQTGKTITIAAKKVVKFKPGKALKEEVGG
CCCCCCCEEEEEEHHHHHCCCCCHHHHHCCC
>Mature Secondary Structure 
SKAELAEKIAEQTGLTKADAERAVNAFINVVTSTLKSGDDVTLVGFGTFTTGDRAERQG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHCC
RNPQTGKTITIAAKKVVKFKPGKALKEEVGG
CCCCCCCEEEEEEHHHHHCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10722605; 10761919 [H]