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The map label for this gene is gcpE

Identifier: 21672988

GI number: 21672988

Start: 148807

End: 151047

Strand: Direct

Name: gcpE

Synonym: CT0147

Alternate gene names: 21672988

Gene position: 148807-151047 (Clockwise)

Preceding gene: 21672987

Following gene: 21672989

Centisome position: 6.91

GC content: 57.74

Gene sequence:

>2241_bases
ATGGTGAACTCAAAAGCATCGTCTTTCCGGTTTTCCTTTTTCATTCATAATTCGGCATTTATTATTCTCTTTCCCCCATC
TTTCTCCCGCCTTTTATTTATCCCGGCCATTGGCTACCTTCAGGATCATCTTCAATCACTTTCAACCAGCCGTCTTATGA
GCGAAGAGCGTCTTGTTTCAGGCAATATCATCGATTATCCGGCGCCAGTGTACAGTTATCGCCGGCGAGTCACCAGGGAA
GTCCCTTTCGGGACGATCTTCCTTGGCGGATATTTGCCGATCCGGGTGGAGTCCATGATTACCGCTCACACGATGGATAC
GGCGGCTTCGGTGGAGCAGTGTCGGCGTTTGTACGAGGCGGGGTGTGAAATCATCAGGTTGACCGTGCCGACCGAGAAAG
ATGCGGAGAATCTCAAAAACATCCGTGAGCAGCTCCGGCGGGATGGTATCGACACGCCGCTGGTGGCGGATATTCATTTT
TCAGCCAAGGCGGCGATGAAGGCGGTGGAGTTCGTCGAGAACATCCGTATCAATCCCGGCAACTACGCCACCGGCGCGAA
GTTTTCGAGCAAGGATTACACCGATGACGAGTATCGCGCTGAACTCGACAAGGTACGTGAGGAGTTCACGCCGCTGGTGC
GAAAGGCCAGGTCGCTTGGCGTCTCCATGCGCATTGGCACCAACCACGGTTCCCTCTCCGACCGCATCGTGAGCCGCTAC
GGCAATTCGCCGGAGGGCATGGTCGAGGCGGCGCTCGAATTCTCCAGAATCTGCGAGGACGAAGGCTATTACGACCAGCT
CTTTTCGATGAAGTCATCGAACGTCAGGGTGATGATCCAGGCCTACCGGCTGCTCGTCGCCCGTGCCGATGCGGAGCTGC
GCTACGCCTACCCGCTGCATCTCGGCGTGACCGAGGCGGGTGATGGCGACGAGGGGCGCATCAAGTCGGCGATGGGCATC
GGCGCTTTGCTCGAAGATGGTCTCGGTGACACTATTCGCGTGTCGCTCACCGAAGATCCGGTCAACGAGGTGCCGGTTGG
TTTCGCTATCGTCAAGAAGTACAACGACATGCTGCTGGTGCGGGGCGACCGGGCGCATCTTCCGGTCAAACACGTGATTG
AGCACGAGCGCAAGAGCGCCGGACATGTGCAGCTGCCGTTCGAGCCGTTCAGCTACTCGCGGCGTCCTTCCATCAGCATC
GATGGTGCGGGCATACCGGTCGGAGGCGATGCCTTGCCCGGGGTTGAAACTGCTGCCCATGCGCCCATCACTGATACGGA
ATCGCTCCGCGACGAGATTCTCGCGAGGCTCGATCCGGGCAAGCCGGAGGATGCGATCCGCTCGGAGCTGGTCAGTGTTG
GCGTCGGTAGTGCCGAAGATATTTCGTTGCTCAAGGCATTGCTCGATTCGCTGGGAAATTTGCGCGAAAAGATTGTCGTT
TCGACTGCCGATACCTCGATTGTTCCGGCGTTGTTGCCGCTCTGCGGGCGAGTGCGGCTCGATATCGTCGAAGGTGAAAC
GCTCGGCACCGGCTTGATTGAATCGCTCCACGACAGGAACGCCGCTATCGAGTTCTGCTTTATCCACGAAAAATCTTCGG
AGAATGTGCCTGCCGAGGTGCTGGTCCGTCTTGCTGCCAAGCTGAAAGCGAGAGGGTTGCAGCGGGTCATGCTCTCTATC
GTTTCCGATGCACCGCTCTATTCTACCAGAAAACTCGCGCTCGAGCTGAAAAAAGCCGGGCTCGACTATCCGATTGCCGT
GCGTTACCGTCGGCTCGACGGTGAGCGCTCCGGCGTGCTCATCCAGAGTGCCATTCAGGCGGGCACCTTGTTCTGCGACG
GTATCGGCGACCTCATTGCGCTTGAGACGAATATGCCAGCCAGCGAAGAGGTGAGCCTTTGCTTTAACATCCTTCAGGCG
GCCAGAATCAGGATGTCCAAAACCGAGTTCATCTCCTGCCCTGGATGCGGACGAACCTACTTCGAGCTTGAGAAGACCAC
GGCGCTCATCAAGCAGCGTGTCTCGCACCTCAAGGGGTTGAAGATCGGCATCATGGGCTGCATCGTCAACGGCCCCGGCG
AAATGGCCGACGCCGATTTCGGCTACGTTGGCTCCGGTAAGGGGCGCGTCAGCCTCTACGTCGGCAAAGAGTGTGTCGAG
GAGAACATCCCGGAGGCAGAGGCGCTTGAGCGGCTGATTGAGCTGATCCGGCAGAACGGCAAGTGGGTTGATCCTGTATG
A

Upstream 100 bases:

>100_bases
GGCTCGGGAACGCTTCAATTTCCACAAAAAAGGCGAGACGGTATTTATCATAAGGGAGAAGTGACCCCGAACGAGGCCTG
AAAGTGGACGTTGTGAACAG

Downstream 100 bases:

>100_bases
GCTTTTATACGCTGATTACGGGCGCGTCCACCGGTATCGGCCGGCGACTTGCTGAAGAGTTTGCCTCGATGGGCGACAAC
CTCGTGCTGGTTGCCCGCTC

Product: GcpE protein

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 746; Mature: 746

Protein sequence:

>746_residues
MVNSKASSFRFSFFIHNSAFIILFPPSFSRLLFIPAIGYLQDHLQSLSTSRLMSEERLVSGNIIDYPAPVYSYRRRVTRE
VPFGTIFLGGYLPIRVESMITAHTMDTAASVEQCRRLYEAGCEIIRLTVPTEKDAENLKNIREQLRRDGIDTPLVADIHF
SAKAAMKAVEFVENIRINPGNYATGAKFSSKDYTDDEYRAELDKVREEFTPLVRKARSLGVSMRIGTNHGSLSDRIVSRY
GNSPEGMVEAALEFSRICEDEGYYDQLFSMKSSNVRVMIQAYRLLVARADAELRYAYPLHLGVTEAGDGDEGRIKSAMGI
GALLEDGLGDTIRVSLTEDPVNEVPVGFAIVKKYNDMLLVRGDRAHLPVKHVIEHERKSAGHVQLPFEPFSYSRRPSISI
DGAGIPVGGDALPGVETAAHAPITDTESLRDEILARLDPGKPEDAIRSELVSVGVGSAEDISLLKALLDSLGNLREKIVV
STADTSIVPALLPLCGRVRLDIVEGETLGTGLIESLHDRNAAIEFCFIHEKSSENVPAEVLVRLAAKLKARGLQRVMLSI
VSDAPLYSTRKLALELKKAGLDYPIAVRYRRLDGERSGVLIQSAIQAGTLFCDGIGDLIALETNMPASEEVSLCFNILQA
ARIRMSKTEFISCPGCGRTYFELEKTTALIKQRVSHLKGLKIGIMGCIVNGPGEMADADFGYVGSGKGRVSLYVGKECVE
ENIPEAEALERLIELIRQNGKWVDPV

Sequences:

>Translated_746_residues
MVNSKASSFRFSFFIHNSAFIILFPPSFSRLLFIPAIGYLQDHLQSLSTSRLMSEERLVSGNIIDYPAPVYSYRRRVTRE
VPFGTIFLGGYLPIRVESMITAHTMDTAASVEQCRRLYEAGCEIIRLTVPTEKDAENLKNIREQLRRDGIDTPLVADIHF
SAKAAMKAVEFVENIRINPGNYATGAKFSSKDYTDDEYRAELDKVREEFTPLVRKARSLGVSMRIGTNHGSLSDRIVSRY
GNSPEGMVEAALEFSRICEDEGYYDQLFSMKSSNVRVMIQAYRLLVARADAELRYAYPLHLGVTEAGDGDEGRIKSAMGI
GALLEDGLGDTIRVSLTEDPVNEVPVGFAIVKKYNDMLLVRGDRAHLPVKHVIEHERKSAGHVQLPFEPFSYSRRPSISI
DGAGIPVGGDALPGVETAAHAPITDTESLRDEILARLDPGKPEDAIRSELVSVGVGSAEDISLLKALLDSLGNLREKIVV
STADTSIVPALLPLCGRVRLDIVEGETLGTGLIESLHDRNAAIEFCFIHEKSSENVPAEVLVRLAAKLKARGLQRVMLSI
VSDAPLYSTRKLALELKKAGLDYPIAVRYRRLDGERSGVLIQSAIQAGTLFCDGIGDLIALETNMPASEEVSLCFNILQA
ARIRMSKTEFISCPGCGRTYFELEKTTALIKQRVSHLKGLKIGIMGCIVNGPGEMADADFGYVGSGKGRVSLYVGKECVE
ENIPEAEALERLIELIRQNGKWVDPV
>Mature_746_residues
MVNSKASSFRFSFFIHNSAFIILFPPSFSRLLFIPAIGYLQDHLQSLSTSRLMSEERLVSGNIIDYPAPVYSYRRRVTRE
VPFGTIFLGGYLPIRVESMITAHTMDTAASVEQCRRLYEAGCEIIRLTVPTEKDAENLKNIREQLRRDGIDTPLVADIHF
SAKAAMKAVEFVENIRINPGNYATGAKFSSKDYTDDEYRAELDKVREEFTPLVRKARSLGVSMRIGTNHGSLSDRIVSRY
GNSPEGMVEAALEFSRICEDEGYYDQLFSMKSSNVRVMIQAYRLLVARADAELRYAYPLHLGVTEAGDGDEGRIKSAMGI
GALLEDGLGDTIRVSLTEDPVNEVPVGFAIVKKYNDMLLVRGDRAHLPVKHVIEHERKSAGHVQLPFEPFSYSRRPSISI
DGAGIPVGGDALPGVETAAHAPITDTESLRDEILARLDPGKPEDAIRSELVSVGVGSAEDISLLKALLDSLGNLREKIVV
STADTSIVPALLPLCGRVRLDIVEGETLGTGLIESLHDRNAAIEFCFIHEKSSENVPAEVLVRLAAKLKARGLQRVMLSI
VSDAPLYSTRKLALELKKAGLDYPIAVRYRRLDGERSGVLIQSAIQAGTLFCDGIGDLIALETNMPASEEVSLCFNILQA
ARIRMSKTEFISCPGCGRTYFELEKTTALIKQRVSHLKGLKIGIMGCIVNGPGEMADADFGYVGSGKGRVSLYVGKECVE
ENIPEAEALERLIELIRQNGKWVDPV

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=288, Percent_Identity=38.1944444444444, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_CHLTE (Q8KG23)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661053.1
- ProteinModelPortal:   Q8KG23
- GeneID:   1007062
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0147
- NMPDR:   fig|194439.1.peg.147
- TIGR:   CT0147
- HOGENOM:   HBG335271
- OMA:   SMRIGTN
- ProtClustDB:   CLSK637188
- BioCyc:   CTEP194439:CT_0147-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR017178
- InterPro:   IPR004588
- PIRSF:   PIRSF037336
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE

EC number: =1.17.7.1

Molecular weight: Translated: 82089; Mature: 82089

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNSKASSFRFSFFIHNSAFIILFPPSFSRLLFIPAIGYLQDHLQSLSTSRLMSEERLVS
CCCCCCCCEEEEEEEECCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNIIDYPAPVYSYRRRVTREVPFGTIFLGGYLPIRVESMITAHTMDTAASVEQCRRLYEA
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHC
GCEIIRLTVPTEKDAENLKNIREQLRRDGIDTPLVADIHFSAKAAMKAVEFVENIRINPG
CCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCC
NYATGAKFSSKDYTDDEYRAELDKVREEFTPLVRKARSLGVSMRIGTNHGSLSDRIVSRY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHC
GNSPEGMVEAALEFSRICEDEGYYDQLFSMKSSNVRVMIQAYRLLVARADAELRYAYPLH
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEEEEE
LGVTEAGDGDEGRIKSAMGIGALLEDGLGDTIRVSLTEDPVNEVPVGFAIVKKYNDMLLV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCEEEE
RGDRAHLPVKHVIEHERKSAGHVQLPFEPFSYSRRPSISIDGAGIPVGGDALPGVETAAH
ECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHCC
APITDTESLRDEILARLDPGKPEDAIRSELVSVGVGSAEDISLLKALLDSLGNLREKIVV
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEE
STADTSIVPALLPLCGRVRLDIVEGETLGTGLIESLHDRNAAIEFCFIHEKSSENVPAEV
EECCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCHHH
LVRLAAKLKARGLQRVMLSIVSDAPLYSTRKLALELKKAGLDYPIAVRYRRLDGERSGVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHH
IQSAIQAGTLFCDGIGDLIALETNMPASEEVSLCFNILQAARIRMSKTEFISCPGCGRTY
HHHHHHHCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHH
FELEKTTALIKQRVSHLKGLKIGIMGCIVNGPGEMADADFGYVGSGKGRVSLYVGKECVE
HHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEECHHHHH
ENIPEAEALERLIELIRQNGKWVDPV
CCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVNSKASSFRFSFFIHNSAFIILFPPSFSRLLFIPAIGYLQDHLQSLSTSRLMSEERLVS
CCCCCCCCEEEEEEEECCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNIIDYPAPVYSYRRRVTREVPFGTIFLGGYLPIRVESMITAHTMDTAASVEQCRRLYEA
CCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHC
GCEIIRLTVPTEKDAENLKNIREQLRRDGIDTPLVADIHFSAKAAMKAVEFVENIRINPG
CCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCC
NYATGAKFSSKDYTDDEYRAELDKVREEFTPLVRKARSLGVSMRIGTNHGSLSDRIVSRY
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHC
GNSPEGMVEAALEFSRICEDEGYYDQLFSMKSSNVRVMIQAYRLLVARADAELRYAYPLH
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCEEEEEEEE
LGVTEAGDGDEGRIKSAMGIGALLEDGLGDTIRVSLTEDPVNEVPVGFAIVKKYNDMLLV
EECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHCCCCCEEEE
RGDRAHLPVKHVIEHERKSAGHVQLPFEPFSYSRRPSISIDGAGIPVGGDALPGVETAAH
ECCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHCC
APITDTESLRDEILARLDPGKPEDAIRSELVSVGVGSAEDISLLKALLDSLGNLREKIVV
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEE
STADTSIVPALLPLCGRVRLDIVEGETLGTGLIESLHDRNAAIEFCFIHEKSSENVPAEV
EECCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCHHH
LVRLAAKLKARGLQRVMLSIVSDAPLYSTRKLALELKKAGLDYPIAVRYRRLDGERSGVL
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHH
IQSAIQAGTLFCDGIGDLIALETNMPASEEVSLCFNILQAARIRMSKTEFISCPGCGRTY
HHHHHHHCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHH
FELEKTTALIKQRVSHLKGLKIGIMGCIVNGPGEMADADFGYVGSGKGRVSLYVGKECVE
HHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEECHHHHH
ENIPEAEALERLIELIRQNGKWVDPV
CCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901