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The map label for this gene is ppnK

Identifier: 21672926

GI number: 21672926

Start: 79791

End: 80642

Strand: Direct

Name: ppnK

Synonym: CT0085

Alternate gene names: 21672926

Gene position: 79791-80642 (Clockwise)

Preceding gene: 21672925

Following gene: 21672927

Centisome position: 3.7

GC content: 57.16

Gene sequence:

>852_bases
ATGAAGTTCGCCATCTTCGTGAACACTACCAGGGAGAAAGCGCTGGAGCTGGCCCGAGAGCTGACGGCGTGGCTCGATGC
GCGGTCGATCGATTACGTCTTCGATCCCCAGTCGGCCAAAGCGCTCGGCTGCGGCAAATGGGAGGAGAAGGCAGACCTCA
GCCAGCACTGCGACGCCTTCGTCGCGCTTGGCGGCGATGGCACGCTTCTGCTTGCTTCGCACTATTCCCGCTCCAAGCCG
GTGGTGGGCATCAATGTGGGCGACCTCGGCTTTCTGACCGAGTTCAGTCCGGACGAAATGTGGGTGGCCATGGATCATCT
GGTGAGCGGTAACTACTCGATCCACACGCGCTCGCAGCTCGAAGCCACGCTTGAATCGGGAGAATCGCTGACTTCGCTCA
ACGACGTTATCTTCGAAAAAGGCTCCGCTGCACGGCGGCTGCCAGCCTTCACCATCCTGCTCGACGATGAAATGCTCGGA
TCATACCGTGCCGATGGCATCATCATCGCCACCTCGACCGGTTCGACGGCCTACTCGATGTCCGCAGGCGGCCCGATCAT
CGCCCCGAAATCGAATGTGTTCGTCATTACCCCGATCTGTCCGCACATGTTGACGGTCAGACCGATCGTTATCAGCGACG
ACAAAACCATCAAAATTTCGGTCGATTCGCAATCCGGGGAGTTTCCGCTGAAAATGGATGGCATCCAGAAAAAACTGCTC
GCTCCTGGTGAAGTGGTCACGGTGAAAAAGTCGCCACACCACATCAATCTGGTGGCCAACGAAAAAAGGAACTACTGCGA
AATCCTTCGCAAAAAGCTGCTCTGGAGTCACGAACATCCCACGGGAGAGTGA

Upstream 100 bases:

>100_bases
ACGTAGCCGGAGAAGTTCGCGGCGGCAACTTCGAGGCGCTCGAATCAAAATTCCGCGAAGCGAACGATCTCTATCAACGC
CTTCAGGAGAGGAGCAGCTC

Downstream 100 bases:

>100_bases
CTTCCACAGTCTTTTCCGATACCCTCTCATGATCGATCAAAGAACGAGTTACCCTTCTTCCATGACCCGACACCTTTCCC
CATTCTGGCTGAACCGGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKFAIFVNTTREKALELARELTAWLDARSIDYVFDPQSAKALGCGKWEEKADLSQHCDAFVALGGDGTLLLASHYSRSKP
VVGINVGDLGFLTEFSPDEMWVAMDHLVSGNYSIHTRSQLEATLESGESLTSLNDVIFEKGSAARRLPAFTILLDDEMLG
SYRADGIIIATSTGSTAYSMSAGGPIIAPKSNVFVITPICPHMLTVRPIVISDDKTIKISVDSQSGEFPLKMDGIQKKLL
APGEVVTVKKSPHHINLVANEKRNYCEILRKKLLWSHEHPTGE

Sequences:

>Translated_283_residues
MKFAIFVNTTREKALELARELTAWLDARSIDYVFDPQSAKALGCGKWEEKADLSQHCDAFVALGGDGTLLLASHYSRSKP
VVGINVGDLGFLTEFSPDEMWVAMDHLVSGNYSIHTRSQLEATLESGESLTSLNDVIFEKGSAARRLPAFTILLDDEMLG
SYRADGIIIATSTGSTAYSMSAGGPIIAPKSNVFVITPICPHMLTVRPIVISDDKTIKISVDSQSGEFPLKMDGIQKKLL
APGEVVTVKKSPHHINLVANEKRNYCEILRKKLLWSHEHPTGE
>Mature_283_residues
MKFAIFVNTTREKALELARELTAWLDARSIDYVFDPQSAKALGCGKWEEKADLSQHCDAFVALGGDGTLLLASHYSRSKP
VVGINVGDLGFLTEFSPDEMWVAMDHLVSGNYSIHTRSQLEATLESGESLTSLNDVIFEKGSAARRLPAFTILLDDEMLG
SYRADGIIIATSTGSTAYSMSAGGPIIAPKSNVFVITPICPHMLTVRPIVISDDKTIKISVDSQSGEFPLKMDGIQKKLL
APGEVVTVKKSPHHINLVANEKRNYCEILRKKLLWSHEHPTGE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=268, Percent_Identity=28.3582089552239, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1788968, Length=271, Percent_Identity=32.4723247232472, Blast_Score=143, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320794, Length=244, Percent_Identity=32.3770491803279, Blast_Score=129, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325068, Length=228, Percent_Identity=33.3333333333333, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6322509, Length=242, Percent_Identity=32.2314049586777, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI28573832, Length=239, Percent_Identity=31.7991631799163, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI28573830, Length=239, Percent_Identity=31.7991631799163, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28573826, Length=239, Percent_Identity=31.7991631799163, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28573828, Length=239, Percent_Identity=31.7991631799163, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI161077047, Length=239, Percent_Identity=31.7991631799163, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129957, Length=279, Percent_Identity=27.2401433691756, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24653422, Length=279, Percent_Identity=27.2401433691756, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281363321, Length=279, Percent_Identity=27.2401433691756, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI281363323, Length=279, Percent_Identity=27.2401433691756, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24653424, Length=279, Percent_Identity=27.2401433691756, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_CHLTE (Q8KG83)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_660991.1
- ProteinModelPortal:   Q8KG83
- SMR:   Q8KG83
- GeneID:   1006506
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0085
- NMPDR:   fig|194439.1.peg.85
- TIGR:   CT0085
- HOGENOM:   HBG713904
- OMA:   VANEKRN
- ProtClustDB:   CLSK637151
- BioCyc:   CTEP194439:CT_0085-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30989; Mature: 30989

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAIFVNTTREKALELARELTAWLDARSIDYVFDPQSAKALGCGKWEEKADLSQHCDAF
CEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCHHCCHHHCCCEE
VALGGDGTLLLASHYSRSKPVVGINVGDLGFLTEFSPDEMWVAMDHLVSGNYSIHTRSQL
EEECCCCEEEEEECCCCCCCEEEEECCCCCEEECCCCCCEEEEEHHHHCCCEEEEEHHHH
EATLESGESLTSLNDVIFEKGSAARRLPAFTILLDDEMLGSYRADGIIIATSTGSTAYSM
HHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCHHHCCCCCCCEEEEECCCCCEEEE
SAGGPIIAPKSNVFVITPICPHMLTVRPIVISDDKTIKISVDSQSGEFPLKMDGIQKKLL
CCCCCEECCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCEEECCHHHHHC
APGEVVTVKKSPHHINLVANEKRNYCEILRKKLLWSHEHPTGE
CCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKFAIFVNTTREKALELARELTAWLDARSIDYVFDPQSAKALGCGKWEEKADLSQHCDAF
CEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEECCCCCCHHCCHHHCCCEE
VALGGDGTLLLASHYSRSKPVVGINVGDLGFLTEFSPDEMWVAMDHLVSGNYSIHTRSQL
EEECCCCEEEEEECCCCCCCEEEEECCCCCEEECCCCCCEEEEEHHHHCCCEEEEEHHHH
EATLESGESLTSLNDVIFEKGSAARRLPAFTILLDDEMLGSYRADGIIIATSTGSTAYSM
HHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCHHHCCCCCCCEEEEECCCCCEEEE
SAGGPIIAPKSNVFVITPICPHMLTVRPIVISDDKTIKISVDSQSGEFPLKMDGIQKKLL
CCCCCEECCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCEEECCHHHHHC
APGEVVTVKKSPHHINLVANEKRNYCEILRKKLLWSHEHPTGE
CCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901