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The map label for this gene is topA [H]

Identifier: 21672896

GI number: 21672896

Start: 54413

End: 56812

Strand: Direct

Name: topA [H]

Synonym: CT0055

Alternate gene names: 21672896

Gene position: 54413-56812 (Clockwise)

Preceding gene: 21672886

Following gene: 21672901

Centisome position: 2.53

GC content: 58.96

Gene sequence:

>2400_bases
ATGGCTTCAAAAAGCGCAACCCCTTCGGCCAGGAACAGGACCCTCATCGTTGTCGAGTCTCCATCCAAGGCGAAAACCAT
CAACAAATATCTGGGCGACGATTACACGGTTTACGCTTCAGTCGGTCATATCAAGGACTTGCCGAAAAAAGAGATCGGCA
TCGACTTTGACAACCACTACGAACCGCGCTATGAAGTGATCGCTGGCAAGGAGAAGGTGGTGCGGCAGCTCAAGAAGCTC
GCCGGGGAGGCCGACAAGGTGCTGATCGCCACCGACCCTGACCGCGAAGGCGAGGCCATTGCCTGGCACATCTCCAACGA
GATCGACTTCGCGAAGAAACCTGTCTTCCGTGTGCTCTTCAACGAGATCACCAGGAACGCCATCATCGAGGCTATCGGCC
ATCCGCAGCAGATCGACTACCGGCTCGTGCGTTCGCAGCAGACCCGGCAGGCTCTTGACAAGATCGTCGGCTATAAGATC
AGTCCATTCCTCTGGAATGTGGTCTATCGAGGCCTTTCGGCAGGCCGTGTGCAGTCCGTGGTGCTTCGGCTGATCTGTGA
GCGTGAAACGGAGATCGAGGCCTTTACGCCACAGGAGTACTGGACGATCTACGCCGACTTCACCACGGAAAATGGAGAGA
CCTTCCGGACAAAGCTGGTAAAAGTCAATGGCGAGAAACCCGACATTACCTCCGGCGAGCAGGCCGAAGCGATCGCGTCA
GCCATTCAGGGCCGGCTGTTCGCGATTAGTGAGATCGTGCCGAAGGTGATCCAGCGCAAGCAGCCGCTACCGTTTACCAC
CTCGCTTTTGCAGCAGGCGGCCTCGAACCAGCTCGGCTTCGGTTCGCAGAAAACGATGCGCACCGCCCAGCAGCTCTACG
AAGGCATCGACCTGGGCTCGGAGGGTGCTACCGGTCTCATCACCTACATGCGTACCGATTCGACCCGCATCAGCGGCGAG
GCGGTCGCTCAGGCGCACGATTACATCTCGCAGCAGTTCGGCCCGGAGTACAAGGGTTTCGGTGGCCAGGGCAAGGCGGG
CAAGAATGCCCAGGACGCGCACGAAGCGATCCGGCCCACCTCGGTGACTCGCACGCCCGAATCGCTCCGCCGTCACCTGA
CGCCCGACCAGTTCCGCCTTTACGAGCTGATCTGGAAGCGCTTCGTGGCCTCGATGATGGCTCCGGCCAAGATTGAGCAG
ACTCGCGTGGATGTGGATGATCAGAAAAAGGAGTTTGTCTTCCGTGCCACCGGCAACAAGGTGCTCTTTCCCGGTTTTTT
CCGTGTCTACGATGACCAGCAGGAGCTTGACTACGAAGCGCAGAAATCTACCCGTGAAGAGCTCGAAAAGGAGCAGATCG
TCAAGCTGCCCGAGCGACTTTCTGTTGACGAACAACTCAAGCTGGCCGAACTCGACCGCCGCCAGAGCTTTACCCGTCCG
CCTGCCCGCTACACCGAGGCGAGTTTGGTCAAGGAGCTCGACAACTACGGTATTGGCCGCCCATCGACCTACGCCTCGAT
CTTCTCGACCCTTCAGGATCGGCGCTACGTCGAGCTACAGAAGAAGAAGATCATCCCGACCGAACTGGGGCGTGATGTCT
CGGCGATTCTCGTGGCCAACTTCCCCGATCTCTTCAATGTCCGCTTCACGGCGGAGATGGAGGACGAGCTCGACAAGGTC
GCCTCGGGTGATGACGAGTACGAAAAGGTGCTCGACCGCTTCTATCGCCCGCTCGAATCGGTTTTGAGCCACCGCAAAAG
CGATCCGCTCATTCCGCAGAACCGCGACGCCGTGCTCTGCGAAAAGTGCGGCAAGGGGCACATGATTGTCAAGTGGACGA
CCAGCGGCAAGTTCCTCGGCTGCTCGAACTATCCCAAGTGCAAGAACATCAAGGCGATTACCACCAACAAGGCCAAACCG
AAGGAGACTGGTGTGCGCTGCCCGGTGTGCGGTGAAGGGCACATGCTCCTGCGCAACGGTCGGCTTGGCCCGTTTCTTGC
CTGCTCCAACTACCCGAAGTGCAATACCCTGCTCAATCTCAGCAAGCAGCGCCACATCGAGCCGATCAAGACCCCGCCCA
TTACCACCGATCTCGCCTGCCCGAAATGCGGCGCGCCGATGTATCTCAGAACCGGCAAACGAGGGCTGTGGCTGGGCTGC
TCGAAATTCCCTAAATGCCGTGGCAGGCTTTCATGGAGCACGCTCGATCCGGAGACGCAGGCCCGCTGGGAAAAAGCGAT
GAACGAGCATATGGCGGCCCATCCGTCCTTGGCCATCACGATGCTCGACGGCAAGCCAGCACCGATGAATTTGCCCATCG
AAGACATCATCGCCCGCGCGGAGGACGCAGGACTCATTAGCGCCACAAGCGAACAGCCGGAGGCTGAAGCCACGACGTGA

Upstream 100 bases:

>100_bases
TGGCAGGCAAGCCCTATCTTTTCCGTCATTATTTGCAAAGGCATCTCCCGGCTGCGGCGTCGGGTTACCCGCAATCCCAA
AGCAACAGGGATCAATCGCA

Downstream 100 bases:

>100_bases
AGCCTTGGGAGTGACGGCTTTAGAGGTCAAAGAGTACCTGTAGGTCATATAAGTCCTATAGGAGTTATATGAAAACGGCT
GCCAGGAAAGACAGCCGTTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 799; Mature: 798

Protein sequence:

>799_residues
MASKSATPSARNRTLIVVESPSKAKTINKYLGDDYTVYASVGHIKDLPKKEIGIDFDNHYEPRYEVIAGKEKVVRQLKKL
AGEADKVLIATDPDREGEAIAWHISNEIDFAKKPVFRVLFNEITRNAIIEAIGHPQQIDYRLVRSQQTRQALDKIVGYKI
SPFLWNVVYRGLSAGRVQSVVLRLICERETEIEAFTPQEYWTIYADFTTENGETFRTKLVKVNGEKPDITSGEQAEAIAS
AIQGRLFAISEIVPKVIQRKQPLPFTTSLLQQAASNQLGFGSQKTMRTAQQLYEGIDLGSEGATGLITYMRTDSTRISGE
AVAQAHDYISQQFGPEYKGFGGQGKAGKNAQDAHEAIRPTSVTRTPESLRRHLTPDQFRLYELIWKRFVASMMAPAKIEQ
TRVDVDDQKKEFVFRATGNKVLFPGFFRVYDDQQELDYEAQKSTREELEKEQIVKLPERLSVDEQLKLAELDRRQSFTRP
PARYTEASLVKELDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGRDVSAILVANFPDLFNVRFTAEMEDELDKV
ASGDDEYEKVLDRFYRPLESVLSHRKSDPLIPQNRDAVLCEKCGKGHMIVKWTTSGKFLGCSNYPKCKNIKAITTNKAKP
KETGVRCPVCGEGHMLLRNGRLGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPITTDLACPKCGAPMYLRTGKRGLWLGC
SKFPKCRGRLSWSTLDPETQARWEKAMNEHMAAHPSLAITMLDGKPAPMNLPIEDIIARAEDAGLISATSEQPEAEATT

Sequences:

>Translated_799_residues
MASKSATPSARNRTLIVVESPSKAKTINKYLGDDYTVYASVGHIKDLPKKEIGIDFDNHYEPRYEVIAGKEKVVRQLKKL
AGEADKVLIATDPDREGEAIAWHISNEIDFAKKPVFRVLFNEITRNAIIEAIGHPQQIDYRLVRSQQTRQALDKIVGYKI
SPFLWNVVYRGLSAGRVQSVVLRLICERETEIEAFTPQEYWTIYADFTTENGETFRTKLVKVNGEKPDITSGEQAEAIAS
AIQGRLFAISEIVPKVIQRKQPLPFTTSLLQQAASNQLGFGSQKTMRTAQQLYEGIDLGSEGATGLITYMRTDSTRISGE
AVAQAHDYISQQFGPEYKGFGGQGKAGKNAQDAHEAIRPTSVTRTPESLRRHLTPDQFRLYELIWKRFVASMMAPAKIEQ
TRVDVDDQKKEFVFRATGNKVLFPGFFRVYDDQQELDYEAQKSTREELEKEQIVKLPERLSVDEQLKLAELDRRQSFTRP
PARYTEASLVKELDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGRDVSAILVANFPDLFNVRFTAEMEDELDKV
ASGDDEYEKVLDRFYRPLESVLSHRKSDPLIPQNRDAVLCEKCGKGHMIVKWTTSGKFLGCSNYPKCKNIKAITTNKAKP
KETGVRCPVCGEGHMLLRNGRLGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPITTDLACPKCGAPMYLRTGKRGLWLGC
SKFPKCRGRLSWSTLDPETQARWEKAMNEHMAAHPSLAITMLDGKPAPMNLPIEDIIARAEDAGLISATSEQPEAEATT
>Mature_798_residues
ASKSATPSARNRTLIVVESPSKAKTINKYLGDDYTVYASVGHIKDLPKKEIGIDFDNHYEPRYEVIAGKEKVVRQLKKLA
GEADKVLIATDPDREGEAIAWHISNEIDFAKKPVFRVLFNEITRNAIIEAIGHPQQIDYRLVRSQQTRQALDKIVGYKIS
PFLWNVVYRGLSAGRVQSVVLRLICERETEIEAFTPQEYWTIYADFTTENGETFRTKLVKVNGEKPDITSGEQAEAIASA
IQGRLFAISEIVPKVIQRKQPLPFTTSLLQQAASNQLGFGSQKTMRTAQQLYEGIDLGSEGATGLITYMRTDSTRISGEA
VAQAHDYISQQFGPEYKGFGGQGKAGKNAQDAHEAIRPTSVTRTPESLRRHLTPDQFRLYELIWKRFVASMMAPAKIEQT
RVDVDDQKKEFVFRATGNKVLFPGFFRVYDDQQELDYEAQKSTREELEKEQIVKLPERLSVDEQLKLAELDRRQSFTRPP
ARYTEASLVKELDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGRDVSAILVANFPDLFNVRFTAEMEDELDKVA
SGDDEYEKVLDRFYRPLESVLSHRKSDPLIPQNRDAVLCEKCGKGHMIVKWTTSGKFLGCSNYPKCKNIKAITTNKAKPK
ETGVRCPVCGEGHMLLRNGRLGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPITTDLACPKCGAPMYLRTGKRGLWLGCS
KFPKCRGRLSWSTLDPETQARWEKAMNEHMAAHPSLAITMLDGKPAPMNLPIEDIIARAEDAGLISATSEQPEAEATT

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=733, Percent_Identity=23.6016371077763, Blast_Score=130, Evalue=6e-30,
Organism=Homo sapiens, GI10835218, Length=623, Percent_Identity=23.5955056179775, Blast_Score=123, Evalue=9e-28,
Organism=Escherichia coli, GI1787529, Length=817, Percent_Identity=37.8212974296206, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1788061, Length=650, Percent_Identity=23.6923076923077, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI48994931, Length=139, Percent_Identity=32.3741007194245, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32563869, Length=757, Percent_Identity=25.0990752972259, Blast_Score=135, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=542, Percent_Identity=23.9852398523985, Blast_Score=113, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323263, Length=601, Percent_Identity=24.9584026622296, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585251, Length=676, Percent_Identity=23.6686390532544, Blast_Score=131, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 89972; Mature: 89840

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKSATPSARNRTLIVVESPSKAKTINKYLGDDYTVYASVGHIKDLPKKEIGIDFDNHY
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHHCCCCCCCC
EPRYEVIAGKEKVVRQLKKLAGEADKVLIATDPDREGEAIAWHISNEIDFAKKPVFRVLF
CCCHHEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHH
NEITRNAIIEAIGHPQQIDYRLVRSQQTRQALDKIVGYKISPFLWNVVYRGLSAGRVQSV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
VLRLICERETEIEAFTPQEYWTIYADFTTENGETFRTKLVKVNGEKPDITSGEQAEAIAS
HHHHHHCCCCCCCEECCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
AIQGRLFAISEIVPKVIQRKQPLPFTTSLLQQAASNQLGFGSQKTMRTAQQLYEGIDLGS
HHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
EGATGLITYMRTDSTRISGEAVAQAHDYISQQFGPEYKGFGGQGKAGKNAQDAHEAIRPT
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
SVTRTPESLRRHLTPDQFRLYELIWKRFVASMMAPAKIEQTRVDVDDQKKEFVFRATGNK
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCE
VLFPGFFRVYDDQQELDYEAQKSTREELEKEQIVKLPERLSVDEQLKLAELDRRQSFTRP
EECCCHHEEECCHHHHCHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCC
PARYTEASLVKELDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGRDVSAILVAN
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEEC
FPDLFNVRFTAEMEDELDKVASGDDEYEKVLDRFYRPLESVLSHRKSDPLIPQNRDAVLC
CCCHHEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
EKCGKGHMIVKWTTSGKFLGCSNYPKCKNIKAITTNKAKPKETGVRCPVCGEGHMLLRNG
HHCCCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCEEEECC
RLGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPITTDLACPKCGAPMYLRTGKRGLWLGC
CCCCEEEECCCCCHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECC
SKFPKCRGRLSWSTLDPETQARWEKAMNEHMAAHPSLAITMLDGKPAPMNLPIEDIIARA
CCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
EDAGLISATSEQPEAEATT
CCCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
ASKSATPSARNRTLIVVESPSKAKTINKYLGDDYTVYASVGHIKDLPKKEIGIDFDNHY
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCEEEEEECCHHHCCCHHHHCCCCCCCC
EPRYEVIAGKEKVVRQLKKLAGEADKVLIATDPDREGEAIAWHISNEIDFAKKPVFRVLF
CCCHHEECCHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHH
NEITRNAIIEAIGHPQQIDYRLVRSQQTRQALDKIVGYKISPFLWNVVYRGLSAGRVQSV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
VLRLICERETEIEAFTPQEYWTIYADFTTENGETFRTKLVKVNGEKPDITSGEQAEAIAS
HHHHHHCCCCCCCEECCCCEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCHHHHHHHH
AIQGRLFAISEIVPKVIQRKQPLPFTTSLLQQAASNQLGFGSQKTMRTAQQLYEGIDLGS
HHHCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
EGATGLITYMRTDSTRISGEAVAQAHDYISQQFGPEYKGFGGQGKAGKNAQDAHEAIRPT
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCC
SVTRTPESLRRHLTPDQFRLYELIWKRFVASMMAPAKIEQTRVDVDDQKKEFVFRATGNK
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECCCE
VLFPGFFRVYDDQQELDYEAQKSTREELEKEQIVKLPERLSVDEQLKLAELDRRQSFTRP
EECCCHHEEECCHHHHCHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCC
PARYTEASLVKELDNYGIGRPSTYASIFSTLQDRRYVELQKKKIIPTELGRDVSAILVAN
CCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEEC
FPDLFNVRFTAEMEDELDKVASGDDEYEKVLDRFYRPLESVLSHRKSDPLIPQNRDAVLC
CCCHHEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
EKCGKGHMIVKWTTSGKFLGCSNYPKCKNIKAITTNKAKPKETGVRCPVCGEGHMLLRNG
HHCCCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCCCHHCCCCCCCCCCCCEEEECC
RLGPFLACSNYPKCNTLLNLSKQRHIEPIKTPPITTDLACPKCGAPMYLRTGKRGLWLGC
CCCCEEEECCCCCHHHHHHCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECC
SKFPKCRGRLSWSTLDPETQARWEKAMNEHMAAHPSLAITMLDGKPAPMNLPIEDIIARA
CCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHH
EDAGLISATSEQPEAEATT
CCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]