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The map label for this gene is amiB [H]

Identifier: 21672895

GI number: 21672895

Start: 52338

End: 53987

Strand: Reverse

Name: amiB [H]

Synonym: CT0054

Alternate gene names: 21672895

Gene position: 53987-52338 (Counterclockwise)

Preceding gene: 21672897

Following gene: 21672894

Centisome position: 2.51

GC content: 57.21

Gene sequence:

>1650_bases
GTGCTCTTGCATGTCACCAATGGCCCTGAACAGGGATATACGATCAAAGTACAGGGAAGTACCCGGCCAGATGGCGTTTT
TCTGGCGGACATCGGTTCCTTTGCCCGCGCCCTTCGCCTCGGCAGCGTATTCGACGGCAAGCAGATGCAGATCGACGAAG
CGTTCGGCGACACAGTAACAAGCTGCATACTCCTGGAAGGCAGCGATTTCGCGGTCATCGCCTCCGCCGCAGGGGACACC
CCGAAACGGGTTTTGCAGCTCGCCTCGGCTCCTGTCATACGACAGGACAAGGTCTATCTGCCGGTTGACCAGGCGTGCCG
GATGTTTACGCTCTGGCTTGGCCGGCAGTTGCGATACAACGCTTCGGAGAACAGAATCGATGCCACGCTCGACAGCCGCA
GGCCGGACGCTTCACTGCAATCCATCGGACGCCTGCCTAATGATGCCCTCCCCCCAAACCCGCCGGAACAGGCCGCGCCG
TCATCGGACAACCGCACCGATGCCCGCGCCGCCGGAGGAAAAACCACGATCGACGACATCAAGATCGAAACCCGTGCAAA
CGGCGCCATTATACGATTTTCAGCTTCCGGCGACATGCGTAATGCCTCTTTTCTCAGGCCGGACAACAGTGGCATCCTCT
ATCTAACCATTGAAAATGCCACTGGAAACCCTGCTCGCCTTGCGAGAAATTATCCGGAAGGGGCAATCAAATCGATCAGC
CCGAAGCTTCTCGACAGCGGGGCCTTGCAGTTCACCATTGCACTCGATATTCCGACCTCTTCGATCAAATCATCGTCGTT
CAAGTATGATGCGGCAAAAAATGATTATGTCATCTCCATCATGAATGATGTCGATGTCGAAGCCATTCATTTGTCGGAAA
AAGAGCGCCGCATCCAGGAGGGGCTGAGCCGTGACGTAGACAAGTGGAAGCTAGACGCAGTGGTCATCGACGCCGGCCAT
GGCGGCAAAGACCCCGGCGCGATCGGTACGCAGGGCACTCACGAAAAGGATGTGGCACTGAATATCGCCCGCGACCTCGG
CATGTTCATCAGGCAGAAGTGGCCCGATGTCAGGGTGATCTACACCCGCAAGGAGGACCGGTTCATTCCACTCAAGGAGC
GCGGCCAGATCGCCAACCGCTACGGCGGCAAGGTGTTCATCAGCATCCACTGCAACTCCGCGCCAAACAACAGCAAGGTG
CGGGGGCCGGAGGTCTATATTCTCGGTCCTCATAAAACGGATGCCGCCCTCAAGGTAGCTATGTTTGAAAACTCGGTCAT
CACTGGCGAGGAGAACTTCGCAGAAAAATACAAAGGCTTTTCCGACGAGTATCTCATCATGAGCAGCATGGCGCAAAGCG
CCTTTGCAACGCAATCGACCGAGCTGGCGCAGGATGTGCTCCAGCGCCTGAGCCGTAACAACAGCAATAATGGCCTCGGC
GTCCGGCAGGCGGGATTCATGGTGCTCTGGACTCCCTCGATGCCAAGCATTCTGGTCGAGACCGGCTACCTGTCCAACCC
CTCCGAAGAAAAGCTCCTGCGCGACCGCAAGGAACAGACCAAGATAGCCTACGCCATTTTCCAGGGGCTGCAGCAGTATC
GCTCACGCTATGAGACCCGGATGACAACGGCGGCGCGTCCGGTCAATTGA

Upstream 100 bases:

>100_bases
AGACTCTTTATCCAGACAATTTATCAGGCCACTGAGCCTCCTGCTGTTTTTTGTGCTTTTTGCCTCCCGCCTCCTGGCCG
GGCCTTCCGGAAAGGAGGCG

Downstream 100 bases:

>100_bases
CGGTTTATCCCAAGCACGGCTCCACTCTTTTCAACGACCCAACAAGTCCCTCCGTGCCATTTGAGCGGAATTTCTTTTTG
GATGATTGTCAACCGTCAGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLLHVTNGPEQGYTIKVQGSTRPDGVFLADIGSFARALRLGSVFDGKQMQIDEAFGDTVTSCILLEGSDFAVIASAAGDT
PKRVLQLASAPVIRQDKVYLPVDQACRMFTLWLGRQLRYNASENRIDATLDSRRPDASLQSIGRLPNDALPPNPPEQAAP
SSDNRTDARAAGGKTTIDDIKIETRANGAIIRFSASGDMRNASFLRPDNSGILYLTIENATGNPARLARNYPEGAIKSIS
PKLLDSGALQFTIALDIPTSSIKSSSFKYDAAKNDYVISIMNDVDVEAIHLSEKERRIQEGLSRDVDKWKLDAVVIDAGH
GGKDPGAIGTQGTHEKDVALNIARDLGMFIRQKWPDVRVIYTRKEDRFIPLKERGQIANRYGGKVFISIHCNSAPNNSKV
RGPEVYILGPHKTDAALKVAMFENSVITGEENFAEKYKGFSDEYLIMSSMAQSAFATQSTELAQDVLQRLSRNNSNNGLG
VRQAGFMVLWTPSMPSILVETGYLSNPSEEKLLRDRKEQTKIAYAIFQGLQQYRSRYETRMTTAARPVN

Sequences:

>Translated_549_residues
MLLHVTNGPEQGYTIKVQGSTRPDGVFLADIGSFARALRLGSVFDGKQMQIDEAFGDTVTSCILLEGSDFAVIASAAGDT
PKRVLQLASAPVIRQDKVYLPVDQACRMFTLWLGRQLRYNASENRIDATLDSRRPDASLQSIGRLPNDALPPNPPEQAAP
SSDNRTDARAAGGKTTIDDIKIETRANGAIIRFSASGDMRNASFLRPDNSGILYLTIENATGNPARLARNYPEGAIKSIS
PKLLDSGALQFTIALDIPTSSIKSSSFKYDAAKNDYVISIMNDVDVEAIHLSEKERRIQEGLSRDVDKWKLDAVVIDAGH
GGKDPGAIGTQGTHEKDVALNIARDLGMFIRQKWPDVRVIYTRKEDRFIPLKERGQIANRYGGKVFISIHCNSAPNNSKV
RGPEVYILGPHKTDAALKVAMFENSVITGEENFAEKYKGFSDEYLIMSSMAQSAFATQSTELAQDVLQRLSRNNSNNGLG
VRQAGFMVLWTPSMPSILVETGYLSNPSEEKLLRDRKEQTKIAYAIFQGLQQYRSRYETRMTTAARPVN
>Mature_549_residues
MLLHVTNGPEQGYTIKVQGSTRPDGVFLADIGSFARALRLGSVFDGKQMQIDEAFGDTVTSCILLEGSDFAVIASAAGDT
PKRVLQLASAPVIRQDKVYLPVDQACRMFTLWLGRQLRYNASENRIDATLDSRRPDASLQSIGRLPNDALPPNPPEQAAP
SSDNRTDARAAGGKTTIDDIKIETRANGAIIRFSASGDMRNASFLRPDNSGILYLTIENATGNPARLARNYPEGAIKSIS
PKLLDSGALQFTIALDIPTSSIKSSSFKYDAAKNDYVISIMNDVDVEAIHLSEKERRIQEGLSRDVDKWKLDAVVIDAGH
GGKDPGAIGTQGTHEKDVALNIARDLGMFIRQKWPDVRVIYTRKEDRFIPLKERGQIANRYGGKVFISIHCNSAPNNSKV
RGPEVYILGPHKTDAALKVAMFENSVITGEENFAEKYKGFSDEYLIMSSMAQSAFATQSTELAQDVLQRLSRNNSNNGLG
VRQAGFMVLWTPSMPSILVETGYLSNPSEEKLLRDRKEQTKIAYAIFQGLQQYRSRYETRMTTAARPVN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=226, Percent_Identity=35.3982300884956, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI87082163, Length=224, Percent_Identity=36.1607142857143, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790611, Length=230, Percent_Identity=33.4782608695652, Blast_Score=116, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 60431; Mature: 60431

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLHVTNGPEQGYTIKVQGSTRPDGVFLADIGSFARALRLGSVFDGKQMQIDEAFGDTVT
CEEEECCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEHHHHCCCEE
SCILLEGSDFAVIASAAGDTPKRVLQLASAPVIRQDKVYLPVDQACRMFTLWLGRQLRYN
EEEEEECCCEEEEEECCCCCHHHHHHHHCCCCEECCEEEECHHHHHHHHHHHHCCCEECC
ASENRIDATLDSRRPDASLQSIGRLPNDALPPNPPEQAAPSSDNRTDARAAGGKTTIDDI
CCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCEEE
KIETRANGAIIRFSASGDMRNASFLRPDNSGILYLTIENATGNPARLARNYPEGAIKSIS
EEEEECCCEEEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHCCCCCHHHHHCC
PKLLDSGALQFTIALDIPTSSIKSSSFKYDAAKNDYVISIMNDVDVEAIHLSEKERRIQE
HHHHCCCCEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEECCCCEEEEECCHHHHHHHH
GLSRDVDKWKLDAVVIDAGHGGKDPGAIGTQGTHEKDVALNIARDLGMFIRQKWPDVRVI
HHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
YTRKEDRFIPLKERGQIANRYGGKVFISIHCNSAPNNSKVRGPEVYILGPHKTDAALKVA
EEECCCCCCCHHHCCCHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEE
MFENSVITGEENFAEKYKGFSDEYLIMSSMAQSAFATQSTELAQDVLQRLSRNNSNNGLG
EECCCEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCE
VRQAGFMVLWTPSMPSILVETGYLSNPSEEKLLRDRKEQTKIAYAIFQGLQQYRSRYETR
EEECCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTTAARPVN
HHCCCCCCC
>Mature Secondary Structure
MLLHVTNGPEQGYTIKVQGSTRPDGVFLADIGSFARALRLGSVFDGKQMQIDEAFGDTVT
CEEEECCCCCCCEEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCEEEHHHHCCCEE
SCILLEGSDFAVIASAAGDTPKRVLQLASAPVIRQDKVYLPVDQACRMFTLWLGRQLRYN
EEEEEECCCEEEEEECCCCCHHHHHHHHCCCCEECCEEEECHHHHHHHHHHHHCCCEECC
ASENRIDATLDSRRPDASLQSIGRLPNDALPPNPPEQAAPSSDNRTDARAAGGKTTIDDI
CCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCHHHCCCCCCCEEE
KIETRANGAIIRFSASGDMRNASFLRPDNSGILYLTIENATGNPARLARNYPEGAIKSIS
EEEEECCCEEEEEECCCCCCCCEEECCCCCCEEEEEEECCCCCHHHHHCCCCCHHHHHCC
PKLLDSGALQFTIALDIPTSSIKSSSFKYDAAKNDYVISIMNDVDVEAIHLSEKERRIQE
HHHHCCCCEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEECCCCEEEEECCHHHHHHHH
GLSRDVDKWKLDAVVIDAGHGGKDPGAIGTQGTHEKDVALNIARDLGMFIRQKWPDVRVI
HHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
YTRKEDRFIPLKERGQIANRYGGKVFISIHCNSAPNNSKVRGPEVYILGPHKTDAALKVA
EEECCCCCCCHHHCCCHHHCCCCEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCEEEEE
MFENSVITGEENFAEKYKGFSDEYLIMSSMAQSAFATQSTELAQDVLQRLSRNNSNNGLG
EECCCEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCE
VRQAGFMVLWTPSMPSILVETGYLSNPSEEKLLRDRKEQTKIAYAIFQGLQQYRSRYETR
EEECCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTTAARPVN
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]