Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 21672879

GI number: 21672879

Start: 35348

End: 36778

Strand: Reverse

Name: murF [H]

Synonym: CT0038

Alternate gene names: 21672879

Gene position: 36778-35348 (Counterclockwise)

Preceding gene: 21672880

Following gene: 21672878

Centisome position: 1.71

GC content: 64.36

Gene sequence:

>1431_bases
GTGAAGATGAAAGGAGCGCTGATGTTCAGCGATTTCGAGCGGGCGGGAACCGTCGTGGCCCGTGACGTTGGCGAGGGGTA
CCGGCTCGACGATCCGGTGGTGGTAATCGATTCTCGCAAGGCGGTCGATGGCGCAGTGTTCGTCGCGCTGCCGGGAGAGC
GCACCGACGGGCACCGCTTCGTCGGCGAGGTGTTCGCCAACGGTGCGTCGTGGGCCGTGGTGTCGCGCGAATGGTTCGTC
GAAAAGGGCGCGGAGCATCAGGGCGACGACCGCCGTTTTCTCGTCGCCGACGATCCGGTCAAGGCGTTCCAGCAGCTCGC
CGCCGCCTACCGGGAGCGCTTCGACATTCCGGTAATCGGCATCGGCGGCAGCAACGGCAAGACCACCACCAAGGAGATGC
TTGCCGCCGTGCTCTCGACCAGCTTCAACGTCCTCGTCACGCAGGGCAACTACAACAACCACCTCGGCGTGCCGCTGACG
CTCTTGCAGATGCGCCGCGACACCGAAGTGGCCGTGATCGAGATGGGCATCAACCATCCCGGCGAAATGGAGTTCCTCTC
TTCGCTTGCCAAACCGACACACGGCCTGTTGACCAACATCGGCCACGAGCATCTCGAATTTTTCGGCTCCCTCGACGGGG
TCGCCGATGCCGAGGCGGCGCTCTTCCGCTACCTCGAAGCGCACGGCGGAACAGCGTTCGTCAATCTCGACGATCACCGC
CTCGCCGCTGCAGGAGCGAGTCTGTCACGCAAGACCGGCTACGGAGCGCAGCCGGGAGCGGGCCGCGCGTGGTGGGCCGA
ACAGATCGGCGCGGACCGGGTGGGCCGCGTTTCGTTCACGCTCTGCTCCGAATCCGGCGTTCACCAGCCAGTGGCGATGC
AGTTTGTCGGGCGACACAACGTCATCAACGCCGTAGCCGCCTCTGCGGTCGGCGCGCACTTTGGCCTCGCCCCGGCGCAC
ATCGCCGAAGGACTTGGCACACTCCTGCCCGCGAAGGGCTGGAAGCGCATGGAGCTGTTCGAAGACGGCGGAATCGTGGT
GCTCAACGACACCTACAACGCCAATCCCGACTCGGTGCGTCTGGCGCTCGATACGCTTGCGGCCATCGAGTGCCGGGGCC
GCCGGATTGCGGTGCTTGGCGACATGCTCGAACTCGGCGACAATTCGGCCATTGAACATGAAAGCATCGGCAGGTATATT
CGCCAACTGCCGCTCGATGCCTGCCTCACCCTGGGTGATGCCGCCCAACTCATCTGCGAGCAGGCGGGCGGGCGCTGCCT
CAGGCACTTCGGGACGATGGACGAGCTTCGCGGCTTCCTGTCGGAGTACGTCCAGCCGGGTGACGCGTTGCTCTTCAAGG
GGTCTCGCGGAATGAAGCTTGAACTGGCGGCGGACGATCTTATCAAACAGAATCAACAGCACTCGATCTGA

Upstream 100 bases:

>100_bases
AGCGTATCAGGAGATCAGGGGCGTGAAGCACCACTTTTCCGACCGCGAGTGTCTGGAGGCATGTTTTGCGCAAATGAAAT
GAGTTAACGGAAGTCAGGGA

Downstream 100 bases:

>100_bases
ACCGGTAATGCTCTATTACATTCTTCGATACATCAACGAACTCTACAGTCTTCCCGGCATGGGGGTCATCGAATATCTGA
CCTTCAGGGCCAGCGCGGCG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate-D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKMKGALMFSDFERAGTVVARDVGEGYRLDDPVVVIDSRKAVDGAVFVALPGERTDGHRFVGEVFANGASWAVVSREWFV
EKGAEHQGDDRRFLVADDPVKAFQQLAAAYRERFDIPVIGIGGSNGKTTTKEMLAAVLSTSFNVLVTQGNYNNHLGVPLT
LLQMRRDTEVAVIEMGINHPGEMEFLSSLAKPTHGLLTNIGHEHLEFFGSLDGVADAEAALFRYLEAHGGTAFVNLDDHR
LAAAGASLSRKTGYGAQPGAGRAWWAEQIGADRVGRVSFTLCSESGVHQPVAMQFVGRHNVINAVAASAVGAHFGLAPAH
IAEGLGTLLPAKGWKRMELFEDGGIVVLNDTYNANPDSVRLALDTLAAIECRGRRIAVLGDMLELGDNSAIEHESIGRYI
RQLPLDACLTLGDAAQLICEQAGGRCLRHFGTMDELRGFLSEYVQPGDALLFKGSRGMKLELAADDLIKQNQQHSI

Sequences:

>Translated_476_residues
MKMKGALMFSDFERAGTVVARDVGEGYRLDDPVVVIDSRKAVDGAVFVALPGERTDGHRFVGEVFANGASWAVVSREWFV
EKGAEHQGDDRRFLVADDPVKAFQQLAAAYRERFDIPVIGIGGSNGKTTTKEMLAAVLSTSFNVLVTQGNYNNHLGVPLT
LLQMRRDTEVAVIEMGINHPGEMEFLSSLAKPTHGLLTNIGHEHLEFFGSLDGVADAEAALFRYLEAHGGTAFVNLDDHR
LAAAGASLSRKTGYGAQPGAGRAWWAEQIGADRVGRVSFTLCSESGVHQPVAMQFVGRHNVINAVAASAVGAHFGLAPAH
IAEGLGTLLPAKGWKRMELFEDGGIVVLNDTYNANPDSVRLALDTLAAIECRGRRIAVLGDMLELGDNSAIEHESIGRYI
RQLPLDACLTLGDAAQLICEQAGGRCLRHFGTMDELRGFLSEYVQPGDALLFKGSRGMKLELAADDLIKQNQQHSI
>Mature_476_residues
MKMKGALMFSDFERAGTVVARDVGEGYRLDDPVVVIDSRKAVDGAVFVALPGERTDGHRFVGEVFANGASWAVVSREWFV
EKGAEHQGDDRRFLVADDPVKAFQQLAAAYRERFDIPVIGIGGSNGKTTTKEMLAAVLSTSFNVLVTQGNYNNHLGVPLT
LLQMRRDTEVAVIEMGINHPGEMEFLSSLAKPTHGLLTNIGHEHLEFFGSLDGVADAEAALFRYLEAHGGTAFVNLDDHR
LAAAGASLSRKTGYGAQPGAGRAWWAEQIGADRVGRVSFTLCSESGVHQPVAMQFVGRHNVINAVAASAVGAHFGLAPAH
IAEGLGTLLPAKGWKRMELFEDGGIVVLNDTYNANPDSVRLALDTLAAIECRGRRIAVLGDMLELGDNSAIEHESIGRYI
RQLPLDACLTLGDAAQLICEQAGGRCLRHFGTMDELRGFLSEYVQPGDALLFKGSRGMKLELAADDLIKQNQQHSI

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=432, Percent_Identity=35.4166666666667, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51349; Mature: 51349

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKGALMFSDFERAGTVVARDVGEGYRLDDPVVVIDSRKAVDGAVFVALPGERTDGHRF
CCCCCCEEEHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHH
VGEVFANGASWAVVSREWFVEKGAEHQGDDRRFLVADDPVKAFQQLAAAYRERFDIPVIG
HHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEE
IGGSNGKTTTKEMLAAVLSTSFNVLVTQGNYNNHLGVPLTLLQMRRDTEVAVIEMGINHP
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEECCCCC
GEMEFLSSLAKPTHGLLTNIGHEHLEFFGSLDGVADAEAALFRYLEAHGGTAFVNLDDHR
CHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCH
LAAAGASLSRKTGYGAQPGAGRAWWAEQIGADRVGRVSFTLCSESGVHQPVAMQFVGRHN
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHCCHH
VINAVAASAVGAHFGLAPAHIAEGLGTLLPAKGWKRMELFEDGGIVVLNDTYNANPDSVR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCCCEE
LALDTLAAIECRGRRIAVLGDMLELGDNSAIEHESIGRYIRQLPLDACLTLGDAAQLICE
HHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QAGGRCLRHFGTMDELRGFLSEYVQPGDALLFKGSRGMKLELAADDLIKQNQQHSI
HHCCHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHCCCCCCC
>Mature Secondary Structure
MKMKGALMFSDFERAGTVVARDVGEGYRLDDPVVVIDSRKAVDGAVFVALPGERTDGHRF
CCCCCCEEEHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCHHH
VGEVFANGASWAVVSREWFVEKGAEHQGDDRRFLVADDPVKAFQQLAAAYRERFDIPVIG
HHHHHCCCCCEEEEEHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEE
IGGSNGKTTTKEMLAAVLSTSFNVLVTQGNYNNHLGVPLTLLQMRRDTEVAVIEMGINHP
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCCEEEEEECCCCC
GEMEFLSSLAKPTHGLLTNIGHEHLEFFGSLDGVADAEAALFRYLEAHGGTAFVNLDDHR
CHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCH
LAAAGASLSRKTGYGAQPGAGRAWWAEQIGADRVGRVSFTLCSESGVHQPVAMQFVGRHN
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHCCHH
VINAVAASAVGAHFGLAPAHIAEGLGTLLPAKGWKRMELFEDGGIVVLNDTYNANPDSVR
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCCCCCCCCEE
LALDTLAAIECRGRRIAVLGDMLELGDNSAIEHESIGRYIRQLPLDACLTLGDAAQLICE
HHHHHHHHHHHCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
QAGGRCLRHFGTMDELRGFLSEYVQPGDALLFKGSRGMKLELAADDLIKQNQQHSI
HHCCHHHHHCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]