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The map label for this gene is murD

Identifier: 21672877

GI number: 21672877

Start: 32835

End: 34232

Strand: Reverse

Name: murD

Synonym: CT0036

Alternate gene names: 21672877

Gene position: 34232-32835 (Counterclockwise)

Preceding gene: 21672878

Following gene: 21672876

Centisome position: 1.59

GC content: 65.81

Gene sequence:

>1398_bases
GTGAAACCGGAAGAGCTGAAAGGGAAAGTGGCGTCGGTGATCGGCGCCGGCAAAAGCGGTGTATCAGCGGCTGGCCTGCT
CGCGCGGGCGGGGGCGCGGCCATTCTTAAGCGAATTTGGCGCGGTCAGCCCGGAGGCGGCGGCAACACTGCGACAACTTG
GCGTGCCGTTCGAAGAGGGCGGCCATTCGGAGCGGGTCTTCGAGGCTGCGCTGTGCATCGTCAGTCCCGGTATTCCGCAA
ACCGTGCCAGTCATCCGCGAAATGCACGCGCGCGGTATTCCCGTGGTGAGCGAGATCGAGCTGGCGAGCTGGTTCTGTCC
GGCCCGCATCATCGGCATCACCGGCACGGACGGCAAGACCACCACGGCCACGCTGCTCCACCGCATCTGCGCGGCGGAGG
GCGAACGGAAGGGATTCCGAGCCTTCAGCGTGGGTAACATCGGCATTCCGTTTTCGTCGGAGGTCCCCGGCATGACGGCG
GCGGACATCGCCGTGCTCGAACTGAGCAGCTACCAGCTCGAAGCGTGCTTCGACTTTCGGCCCAACATCGCCGTTTTGAC
CAACGTCACGCCCGACCACATGGATCGCTACGGCGGCAGCATCGAAGCCTACGCCACTGCGAAGTATCGCATCCACGCCC
GGCAGGGCGCGGGCGACACCCTCATCTATAATCATGACGATCCGATCCTGCGCGCCCATTTCGACCGTTCCGAACCGTGG
CCCTTCCGCTTGGTGCGCCTCGGTCTGCGGGCCGAAACGCTCGACGTCGCGCCGGGAGATTTCGTCTCCGTCGAAGACGG
CGAGATCGTCGTCCGCGCGTCCGGATCGACGGAGCGGCTCATGCGGGTTGACGAGATCATGAAGCCGGGATTTCGCGGCG
AACACAATCTCTACAACGCGCTCTCCTCCGTCGCAGCGGCGCTGGCCGCTGGTGTTGCGCCGGAGACGATGCGGGGCGTG
CTCGCCGGGTTCGGCGGCGTGGAGCACCGGCAGGAGCTTGCCGGAAACGCCTGCGGCCTCAACTGGATCAACGACTCGAA
GGCCACCAGCGTCAATGCGCTTCGGCAAGCGCTTCAGTCGGTTCCGGCGGGCATGGTGCTCATCGCCGGGGGACGCGACA
AGGGCAACGACTACAGCGCCATCGCCGACCTCGTGCGCGAGAAGGTCGCCTGCATCGTGGCGATTGGCGAGTCGCGCCGG
AAGATCGCCGACGCGTTCCGTGGCGTCACACCGGTCGTCGAGGCCGCTTCGCTCGCGGAGGCGGTCGAGCTGGCTCGCCA
GAACGCCCGTCCCGGCGCGAGCGTGCTCTTTTCGCCAGCCTGTTCGAGCTTTGACATGTTCCGAGACTTCGAGGATCGCG
GGCGCCAGTTCAAGCAACTTGTCCGGGAGCTGACATGA

Upstream 100 bases:

>100_bases
CAGCTGAAGGGATGGGCGGAACAGAAGATCGTGATCCGTTTCTGGATCATTTCAATCCTGCTCTTTCTGACAAGCCTCAT
GACCCTGAAACTGCGATAAC

Downstream 100 bases:

>100_bases
AGCTGAATGACCTTAAACTGAATTTCGATCTGGAGCTTGACGACGCTCCCGCCGAATCGATGGCGTCGCTGCTGCCCTCG
ACCGGACGGGGCGAAAACAT

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKPEELKGKVASVIGAGKSGVSAAGLLARAGARPFLSEFGAVSPEAAATLRQLGVPFEEGGHSERVFEAALCIVSPGIPQ
TVPVIREMHARGIPVVSEIELASWFCPARIIGITGTDGKTTTATLLHRICAAEGERKGFRAFSVGNIGIPFSSEVPGMTA
ADIAVLELSSYQLEACFDFRPNIAVLTNVTPDHMDRYGGSIEAYATAKYRIHARQGAGDTLIYNHDDPILRAHFDRSEPW
PFRLVRLGLRAETLDVAPGDFVSVEDGEIVVRASGSTERLMRVDEIMKPGFRGEHNLYNALSSVAAALAAGVAPETMRGV
LAGFGGVEHRQELAGNACGLNWINDSKATSVNALRQALQSVPAGMVLIAGGRDKGNDYSAIADLVREKVACIVAIGESRR
KIADAFRGVTPVVEAASLAEAVELARQNARPGASVLFSPACSSFDMFRDFEDRGRQFKQLVRELT

Sequences:

>Translated_465_residues
MKPEELKGKVASVIGAGKSGVSAAGLLARAGARPFLSEFGAVSPEAAATLRQLGVPFEEGGHSERVFEAALCIVSPGIPQ
TVPVIREMHARGIPVVSEIELASWFCPARIIGITGTDGKTTTATLLHRICAAEGERKGFRAFSVGNIGIPFSSEVPGMTA
ADIAVLELSSYQLEACFDFRPNIAVLTNVTPDHMDRYGGSIEAYATAKYRIHARQGAGDTLIYNHDDPILRAHFDRSEPW
PFRLVRLGLRAETLDVAPGDFVSVEDGEIVVRASGSTERLMRVDEIMKPGFRGEHNLYNALSSVAAALAAGVAPETMRGV
LAGFGGVEHRQELAGNACGLNWINDSKATSVNALRQALQSVPAGMVLIAGGRDKGNDYSAIADLVREKVACIVAIGESRR
KIADAFRGVTPVVEAASLAEAVELARQNARPGASVLFSPACSSFDMFRDFEDRGRQFKQLVRELT
>Mature_465_residues
MKPEELKGKVASVIGAGKSGVSAAGLLARAGARPFLSEFGAVSPEAAATLRQLGVPFEEGGHSERVFEAALCIVSPGIPQ
TVPVIREMHARGIPVVSEIELASWFCPARIIGITGTDGKTTTATLLHRICAAEGERKGFRAFSVGNIGIPFSSEVPGMTA
ADIAVLELSSYQLEACFDFRPNIAVLTNVTPDHMDRYGGSIEAYATAKYRIHARQGAGDTLIYNHDDPILRAHFDRSEPW
PFRLVRLGLRAETLDVAPGDFVSVEDGEIVVRASGSTERLMRVDEIMKPGFRGEHNLYNALSSVAAALAAGVAPETMRGV
LAGFGGVEHRQELAGNACGLNWINDSKATSVNALRQALQSVPAGMVLIAGGRDKGNDYSAIADLVREKVACIVAIGESRR
KIADAFRGVTPVVEAASLAEAVELARQNARPGASVLFSPACSSFDMFRDFEDRGRQFKQLVRELT

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=465, Percent_Identity=31.6129032258064, Blast_Score=169, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_CHLTE (Q8KGD2)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_660942.1
- ProteinModelPortal:   Q8KGD2
- SMR:   Q8KGD2
- GeneID:   1006454
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0036
- NMPDR:   fig|194439.1.peg.36
- TIGR:   CT0036
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   CLSK637119
- BioCyc:   CTEP194439:CT_0036-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49631; Mature: 49631

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEELKGKVASVIGAGKSGVSAAGLLARAGARPFLSEFGAVSPEAAATLRQLGVPFEEG
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCHHCC
GHSERVFEAALCIVSPGIPQTVPVIREMHARGIPVVSEIELASWFCPARIIGITGTDGKT
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCH
TTATLLHRICAAEGERKGFRAFSVGNIGIPFSSEVPGMTAADIAVLELSSYQLEACFDFR
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEEEECCCEEEEEECCC
PNIAVLTNVTPDHMDRYGGSIEAYATAKYRIHARQGAGDTLIYNHDDPILRAHFDRSEPW
CCEEEEECCCHHHHHHCCCCEEEEEEEEEEEEEECCCCCEEEEECCCCEEEEECCCCCCC
PFRLVRLGLRAETLDVAPGDFVSVEDGEIVVRASGSTERLMRVDEIMKPGFRGEHNLYNA
CHHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHH
LSSVAAALAAGVAPETMRGVLAGFGGVEHRQELAGNACGLNWINDSKATSVNALRQALQS
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
VPAGMVLIAGGRDKGNDYSAIADLVREKVACIVAIGESRRKIADAFRGVTPVVEAASLAE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCHHHHHHHHHHH
AVELARQNARPGASVLFSPACSSFDMFRDFEDRGRQFKQLVRELT
HHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPEELKGKVASVIGAGKSGVSAAGLLARAGARPFLSEFGAVSPEAAATLRQLGVPFEEG
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCHHCC
GHSERVFEAALCIVSPGIPQTVPVIREMHARGIPVVSEIELASWFCPARIIGITGTDGKT
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCH
TTATLLHRICAAEGERKGFRAFSVGNIGIPFSSEVPGMTAADIAVLELSSYQLEACFDFR
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHEEEEEECCCEEEEEECCC
PNIAVLTNVTPDHMDRYGGSIEAYATAKYRIHARQGAGDTLIYNHDDPILRAHFDRSEPW
CCEEEEECCCHHHHHHCCCCEEEEEEEEEEEEEECCCCCEEEEECCCCEEEEECCCCCCC
PFRLVRLGLRAETLDVAPGDFVSVEDGEIVVRASGSTERLMRVDEIMKPGFRGEHNLYNA
CHHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHH
LSSVAAALAAGVAPETMRGVLAGFGGVEHRQELAGNACGLNWINDSKATSVNALRQALQS
HHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
VPAGMVLIAGGRDKGNDYSAIADLVREKVACIVAIGESRRKIADAFRGVTPVVEAASLAE
CCCCEEEEECCCCCCCCHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCHHHHHHHHHHH
AVELARQNARPGASVLFSPACSSFDMFRDFEDRGRQFKQLVRELT
HHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901