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The map label for this gene is nifS

Identifier: 21672833

GI number: 21672833

Start: 634610

End: 635824

Strand: Direct

Name: nifS

Synonym: BUsg577

Alternate gene names: 21672833

Gene position: 634610-635824 (Clockwise)

Preceding gene: 21672827

Following gene: 21672834

Centisome position: 98.93

GC content: 30.53

Gene sequence:

>1215_bases
GTGAAAAATCCAATTTATTTAGATTATGCGGCTACTACACCAGTAGAATTTAAAGTTGCAAAAAAAATGATGAATTATCT
AACAATAGATGGAATATTTGGTAATCCCGCATCCCGTTCTCATAAATTTGGCTGGGAAGCTGAAGAAGTAATTGACATTG
CTCGTAATGAAATATCAGAGTTAATTGGTTCAGATTCTCGAGAAATTGTATTCACTTCTGGTGCTACTGAAGCAAATAAT
CTAGCTATCAAAGGTATTGCATTTTTTCATAGAAAAAAGGGTAATCATATTATTACAAGTAAAACAGAACACAAGTCTGT
ATTAGATGCATGTAGGTATTTAGAAAGCGTGGGTTTTTCTGTAACTTATCTTACTCCTAAAAATAACGGTATTATTGATT
TAAATGATTTAAAAAAAAATATAAATAAAAACACTATACTTGTTTCAATAATGCATGTAAATAATGAAACTGGCATTATA
CAGGATATAACCAATATATCTAAAATTTGCAGATCCCATGACATTTTTTTTCATGTCGACGCCACTCAAAGTGTAGGTAA
AATAGCTATTAATCTAAAAAATTTATTTGTAGATTTAATGTCTTTTTCAGCTCATAAAATATACGGACCTAAGGGAATTG
GAGGCTTATATGTACGTCGAAAACCACGTGTACGTTTATCAGCTTTATCACATGGAGGAGGACATGAAAGAGGAATGCGC
GCAGGAACTTTACCTGTTCATCAAATTGTTGGCATGGGTGAGTCTTTTAAATTAGCTAGAAAAAAAATAAATGATGATTT
TATTCATTTAACTAATTTAAGAAACGATCTTTGGAATGGTATTAAAAATATTGAAGAGGTTTATTTAAATAGTGATTTAA
AGCAAGGTGCACCTCATATTCTAAATGTTAGCTTTAACTATATTGAAGGTGAGTCATTAATTATGGCACTTAAAGATTTA
GCTATTTCTTCTGGTTCCGCATGTACTTCTTCAAGTTTAGAGCCATCTTATGTTTTAAGAGCTTTAGGAATTAAAGATGA
ATTAGCTCATAGTTCCATTCGTTTTTCAATTGGAAGATTTACAACAAAAGAAGAAATTCAACATACAGTAAAATCAGTAC
ATAAATCAATTCATAGATTACGTGCTCTTTCACCTTTGTGGGAAATGTTTAAATCAGGAGTTGATTTAAATAGTATAGAA
TGGGATCATACCTAA

Upstream 100 bases:

>100_bases
TTTACATTTATTTTTAGATCTAAATATTTTTTAAAATGATAGAATATTACTTTAATCTTTAGTTCTTTTTCTACAATGAT
TATATAAGGTAAATAATTTA

Downstream 100 bases:

>100_bases
AGTAAGAGAATCAAATATATATTAAGAAGGTAGTTAAAATGGCTTATAGCAAAAAAGTTATGGATCATTATGAAAATCCA
CGTAATGTTGGTTCCTTTTC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKNPIYLDYAATTPVEFKVAKKMMNYLTIDGIFGNPASRSHKFGWEAEEVIDIARNEISELIGSDSREIVFTSGATEANN
LAIKGIAFFHRKKGNHIITSKTEHKSVLDACRYLESVGFSVTYLTPKNNGIIDLNDLKKNINKNTILVSIMHVNNETGII
QDITNISKICRSHDIFFHVDATQSVGKIAINLKNLFVDLMSFSAHKIYGPKGIGGLYVRRKPRVRLSALSHGGGHERGMR
AGTLPVHQIVGMGESFKLARKKINDDFIHLTNLRNDLWNGIKNIEEVYLNSDLKQGAPHILNVSFNYIEGESLIMALKDL
AISSGSACTSSSLEPSYVLRALGIKDELAHSSIRFSIGRFTTKEEIQHTVKSVHKSIHRLRALSPLWEMFKSGVDLNSIE
WDHT

Sequences:

>Translated_404_residues
MKNPIYLDYAATTPVEFKVAKKMMNYLTIDGIFGNPASRSHKFGWEAEEVIDIARNEISELIGSDSREIVFTSGATEANN
LAIKGIAFFHRKKGNHIITSKTEHKSVLDACRYLESVGFSVTYLTPKNNGIIDLNDLKKNINKNTILVSIMHVNNETGII
QDITNISKICRSHDIFFHVDATQSVGKIAINLKNLFVDLMSFSAHKIYGPKGIGGLYVRRKPRVRLSALSHGGGHERGMR
AGTLPVHQIVGMGESFKLARKKINDDFIHLTNLRNDLWNGIKNIEEVYLNSDLKQGAPHILNVSFNYIEGESLIMALKDL
AISSGSACTSSSLEPSYVLRALGIKDELAHSSIRFSIGRFTTKEEIQHTVKSVHKSIHRLRALSPLWEMFKSGVDLNSIE
WDHT
>Mature_404_residues
MKNPIYLDYAATTPVEFKVAKKMMNYLTIDGIFGNPASRSHKFGWEAEEVIDIARNEISELIGSDSREIVFTSGATEANN
LAIKGIAFFHRKKGNHIITSKTEHKSVLDACRYLESVGFSVTYLTPKNNGIIDLNDLKKNINKNTILVSIMHVNNETGII
QDITNISKICRSHDIFFHVDATQSVGKIAINLKNLFVDLMSFSAHKIYGPKGIGGLYVRRKPRVRLSALSHGGGHERGMR
AGTLPVHQIVGMGESFKLARKKINDDFIHLTNLRNDLWNGIKNIEEVYLNSDLKQGAPHILNVSFNYIEGESLIMALKDL
AISSGSACTSSSLEPSYVLRALGIKDELAHSSIRFSIGRFTTKEEIQHTVKSVHKSIHRLRALSPLWEMFKSGVDLNSIE
WDHT

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=53.25, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=30.4347826086957, Blast_Score=182, Evalue=3e-46,
Organism=Escherichia coli, GI48994898, Length=403, Percent_Identity=72.2084367245658, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=407, Percent_Identity=23.5872235872236, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787970, Length=195, Percent_Identity=28.7179487179487, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=53.8653366583541, Blast_Score=461, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=33.2307692307692, Blast_Score=137, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=398, Percent_Identity=52.0100502512563, Blast_Score=442, Evalue=1e-125,
Organism=Drosophila melanogaster, GI20129463, Length=401, Percent_Identity=53.1172069825436, Blast_Score=455, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_BUCAP (O51886)

Other databases:

- EMBL:   AF008210
- EMBL:   AE013218
- RefSeq:   NP_660900.1
- ProteinModelPortal:   O51886
- SMR:   O51886
- EnsemblBacteria:   EBBUCT00000000452
- GeneID:   1005620
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg577
- GeneTree:   EBGT00050000007765
- HOGENOM:   HBG635316
- OMA:   RHKPRIR
- ProtClustDB:   PRK14012
- BioCyc:   BAPH198804:BUSG577-MONOMER
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 45144; Mature: 45144

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPIYLDYAATTPVEFKVAKKMMNYLTIDGIFGNPASRSHKFGWEAEEVIDIARNEISE
CCCCEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGSDSREIVFTSGATEANNLAIKGIAFFHRKKGNHIITSKTEHKSVLDACRYLESVGFS
HHCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCE
VTYLTPKNNGIIDLNDLKKNINKNTILVSIMHVNNETGIIQDITNISKICRSHDIFFHVD
EEEEECCCCCEEEHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
ATQSVGKIAINLKNLFVDLMSFSAHKIYGPKGIGGLYVRRKPRVRLSALSHGGGHERGMR
CCCCCCCEEEHHHHHHHHHHHCCHHEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCC
AGTLPVHQIVGMGESFKLARKKINDDFIHLTNLRNDLWNGIKNIEEVYLNSDLKQGAPHI
CCCCHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCEE
LNVSFNYIEGESLIMALKDLAISSGSACTSSSLEPSYVLRALGIKDELAHSSIRFSIGRF
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHEEECCCC
TTKEEIQHTVKSVHKSIHRLRALSPLWEMFKSGVDLNSIEWDHT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKNPIYLDYAATTPVEFKVAKKMMNYLTIDGIFGNPASRSHKFGWEAEEVIDIARNEISE
CCCCEEEEECCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LIGSDSREIVFTSGATEANNLAIKGIAFFHRKKGNHIITSKTEHKSVLDACRYLESVGFS
HHCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHCCCE
VTYLTPKNNGIIDLNDLKKNINKNTILVSIMHVNNETGIIQDITNISKICRSHDIFFHVD
EEEEECCCCCEEEHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEE
ATQSVGKIAINLKNLFVDLMSFSAHKIYGPKGIGGLYVRRKPRVRLSALSHGGGHERGMR
CCCCCCCEEEHHHHHHHHHHHCCHHEECCCCCCCCEEEECCCCEEEEEECCCCCCCCCCC
AGTLPVHQIVGMGESFKLARKKINDDFIHLTNLRNDLWNGIKNIEEVYLNSDLKQGAPHI
CCCCHHHHHHCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCEE
LNVSFNYIEGESLIMALKDLAISSGSACTSSSLEPSYVLRALGIKDELAHSSIRFSIGRF
EEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHEEECCCC
TTKEEIQHTVKSVHKSIHRLRALSPLWEMFKSGVDLNSIEWDHT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9516544; 12089438