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The map label for this gene is hslU

Identifier: 21672818

GI number: 21672818

Start: 613489

End: 614820

Strand: Direct

Name: hslU

Synonym: BUsg558

Alternate gene names: 21672818

Gene position: 613489-614820 (Clockwise)

Preceding gene: 21672817

Following gene: 21672819

Centisome position: 95.64

GC content: 30.48

Gene sequence:

>1332_bases
ATGTCTGAAATGACACCCCCTCAAATTGTTTCTGAACTTAATAAATTTATTATTGGTCAAGAACAAGCAAAAAAAGCTGT
TGCTATTGCATTAAGAAATCGTTGGAGGAGAATGCAATTAAATAACGAGTTACGTCATGAAATTACTCCTAAAAATATCT
TGATGATTGGTCCTACTGGAGTAGGAAAAACAGAAATTGCTAGAAGATTGGCAAAATTAGCAAATTCACCTTTTATAAAA
GTAGAAGCAACTAAATTTACTGAAGTTGGATACGTTGGAAAAGAAGTAGACTCAATTATTCGAGATTTAACCGATGCAGC
TATTAAAATGATTCGAGTAAAAAACATTGAAAAAAACAAACTTCGAGTCGAAGAAATAGTTGAAGAAAGAATACTAGACG
TTCTAGTACCTAGACCGAAAAATAACTGGACAGAAAATGAAAAAAATGAAAGTTTATTAAATACATTGCAAGTATTTCGA
AAAAAATTAAGAGAAGGAATTCTCGACGAAAAAGAAATTGAAATTAATGTATTAGCTAGCACTATGGGAGTTGAAATAAT
GGCGCCTCCTGGTATGGAAGAGTTAACAAGTCAATTGCAATCTCTTTTTCAAAATTTAGGAGGGCATAAAAAAAATACAA
GAAGACTAAAAATAAAAGATGCAGTTTTGTTATTAAAAGAAGAAGAAGCTGCTAAATTAATAAATCAAGAAGAAATTAAA
AAAGAAGCAATTAATGCAGTAGAACAAAATGGTATAGTATTTGTTGATGAAATTGATAAAATATGTAAAAGACGTGATTC
TTCTGGTCCAGATGTTTCTAGAGAAGGTGTGCAAAGAGATTTACTCCCTTTAGTTGAAGGTTGCACTGTATCAACTAAGT
ATGGCATGGTTAAAACAGATCATATTTTATTTATTGCATCAGGTGCTTTTCAAACATCAACACCTTCTGATTTGATTCCC
GAGTTACAAGGACGTCTTCCGATCAAAGTAGAATTACAACCATTAACAATTAATGATTTTGAAAAAATTCTAACTGAACC
AACTGCATCTATTACAGCACAATATAAAGCTCTTATGAAAACAGAAGGTGTATGCATCAATTTTACAAAAGAAGGAATAC
GAAATATTGCAGAAGCAGCTTGGAAAGTTAATGAATCTATGGAAAATATTGGAGCACGTCGTTTACATACAATATTAGAA
AAACTTATGGAAGATATTTCTTTTAATGCTTGTGATAACGTTGGTAAAACTATTGAAATTAACTCAGAATATGTTGGAAA
ACATTTAGATCAACTAATATCTAACGAAGATTTAGGTAGATTTATTTTATAA

Upstream 100 bases:

>100_bases
TCGTAGAAAAATCATTAAATATTGCAGCAAATATTTGTATATATACCAATCATAGTTTTACTATAAAAGAACTATTTTCA
GAAAAATAAGGATTATTTAT

Downstream 100 bases:

>100_bases
TTTTTAAAATTCTAATCTATTTTTTAATAAATTAAAACAGATGAATGTTTTTTAAAAAAATATAAATATTTTTATTATTT
ATTACAAATAGTTGTTGAAA

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSEMTPPQIVSELNKFIIGQEQAKKAVAIALRNRWRRMQLNNELRHEITPKNILMIGPTGVGKTEIARRLAKLANSPFIK
VEATKFTEVGYVGKEVDSIIRDLTDAAIKMIRVKNIEKNKLRVEEIVEERILDVLVPRPKNNWTENEKNESLLNTLQVFR
KKLREGILDEKEIEINVLASTMGVEIMAPPGMEELTSQLQSLFQNLGGHKKNTRRLKIKDAVLLLKEEEAAKLINQEEIK
KEAINAVEQNGIVFVDEIDKICKRRDSSGPDVSREGVQRDLLPLVEGCTVSTKYGMVKTDHILFIASGAFQTSTPSDLIP
ELQGRLPIKVELQPLTINDFEKILTEPTASITAQYKALMKTEGVCINFTKEGIRNIAEAAWKVNESMENIGARRLHTILE
KLMEDISFNACDNVGKTIEINSEYVGKHLDQLISNEDLGRFIL

Sequences:

>Translated_443_residues
MSEMTPPQIVSELNKFIIGQEQAKKAVAIALRNRWRRMQLNNELRHEITPKNILMIGPTGVGKTEIARRLAKLANSPFIK
VEATKFTEVGYVGKEVDSIIRDLTDAAIKMIRVKNIEKNKLRVEEIVEERILDVLVPRPKNNWTENEKNESLLNTLQVFR
KKLREGILDEKEIEINVLASTMGVEIMAPPGMEELTSQLQSLFQNLGGHKKNTRRLKIKDAVLLLKEEEAAKLINQEEIK
KEAINAVEQNGIVFVDEIDKICKRRDSSGPDVSREGVQRDLLPLVEGCTVSTKYGMVKTDHILFIASGAFQTSTPSDLIP
ELQGRLPIKVELQPLTINDFEKILTEPTASITAQYKALMKTEGVCINFTKEGIRNIAEAAWKVNESMENIGARRLHTILE
KLMEDISFNACDNVGKTIEINSEYVGKHLDQLISNEDLGRFIL
>Mature_442_residues
SEMTPPQIVSELNKFIIGQEQAKKAVAIALRNRWRRMQLNNELRHEITPKNILMIGPTGVGKTEIARRLAKLANSPFIKV
EATKFTEVGYVGKEVDSIIRDLTDAAIKMIRVKNIEKNKLRVEEIVEERILDVLVPRPKNNWTENEKNESLLNTLQVFRK
KLREGILDEKEIEINVLASTMGVEIMAPPGMEELTSQLQSLFQNLGGHKKNTRRLKIKDAVLLLKEEEAAKLINQEEIKK
EAINAVEQNGIVFVDEIDKICKRRDSSGPDVSREGVQRDLLPLVEGCTVSTKYGMVKTDHILFIASGAFQTSTPSDLIPE
LQGRLPIKVELQPLTINDFEKILTEPTASITAQYKALMKTEGVCINFTKEGIRNIAEAAWKVNESMENIGARRLHTILEK
LMEDISFNACDNVGKTIEINSEYVGKHLDQLISNEDLGRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=76.7494356659142, Blast_Score=710, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=101, Percent_Identity=46.5346534653465, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=107, Percent_Identity=36.4485981308411, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648289, Length=248, Percent_Identity=27.4193548387097, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648291, Length=248, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_BUCAP (Q9Z617)

Other databases:

- EMBL:   AE013218
- EMBL:   AF108665
- RefSeq:   NP_660885.1
- ProteinModelPortal:   Q9Z617
- SMR:   Q9Z617
- EnsemblBacteria:   EBBUCT00000000184
- GeneID:   1005633
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg558
- GeneTree:   EBGT00050000007932
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   BAPH198804:BUSG558-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 50031; Mature: 49900

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 256-256 BINDING 321-321 BINDING 393-393

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPPQIVSELNKFIIGQEQAKKAVAIALRNRWRRMQLNNELRHEITPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANSPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAIKMIRVKNIEKNK
CCHHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRVEEIVEERILDVLVPRPKNNWTENEKNESLLNTLQVFRKKLREGILDEKEIEINVLAS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEH
TMGVEIMAPPGMEELTSQLQSLFQNLGGHKKNTRRLKIKDAVLLLKEEEAAKLINQEEIK
HHCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHEEEEECHHHHHHHCHHHHH
KEAINAVEQNGIVFVDEIDKICKRRDSSGPDVSREGVQRDLLPLVEGCTVSTKYGMVKTD
HHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCEEEC
HILFIASGAFQTSTPSDLIPELQGRLPIKVELQPLTINDFEKILTEPTASITAQYKALMK
EEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHH
TEGVCINFTKEGIRNIAEAAWKVNESMENIGARRLHTILEKLMEDISFNACDNVGKTIEI
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
NSEYVGKHLDQLISNEDLGRFIL
CHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SEMTPPQIVSELNKFIIGQEQAKKAVAIALRNRWRRMQLNNELRHEITPKNILMIGPTG
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANSPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAIKMIRVKNIEKNK
CCHHHHHHHHHHHHCCCEEEEEECHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LRVEEIVEERILDVLVPRPKNNWTENEKNESLLNTLQVFRKKLREGILDEKEIEINVLAS
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEH
TMGVEIMAPPGMEELTSQLQSLFQNLGGHKKNTRRLKIKDAVLLLKEEEAAKLINQEEIK
HHCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHEEEEECHHHHHHHCHHHHH
KEAINAVEQNGIVFVDEIDKICKRRDSSGPDVSREGVQRDLLPLVEGCTVSTKYGMVKTD
HHHHHHHHHCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCEEEC
HILFIASGAFQTSTPSDLIPELQGRLPIKVELQPLTINDFEKILTEPTASITAQYKALMK
EEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHHH
TEGVCINFTKEGIRNIAEAAWKVNESMENIGARRLHTILEKLMEDISFNACDNVGKTIEI
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
NSEYVGKHLDQLISNEDLGRFIL
CHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438