Click here to switch to the map view.

The map label for this gene is trpS

Identifier: 21672782

GI number: 21672782

Start: 570794

End: 571804

Strand: Reverse

Name: trpS

Synonym: BUsg517

Alternate gene names: 21672782

Gene position: 571804-570794 (Counterclockwise)

Preceding gene: 21672783

Following gene: 21672780

Centisome position: 89.14

GC content: 23.24

Gene sequence:

>1011_bases
ATGATTAATTCTAAACCAATACTATTCAGTGCCATACAGCCTTCCGGAAACTTAACTATTGGAAATTACATAGGAACAAT
GCGTTATTGGTCTTCATTTCAAGATGATTATGAATGTTTATATTGTATTGCTGATTTACATTCTTTAACTACATTAAATA
AAAACTTTTCTTTAAAAAAATCAATTTTAGATACATTAGCATTATATTTAGCTTGTGGAGTCAATCCAAAGAAAAGTATT
ATTTTTATTCAATCACATGTGTATCAACATAGTCAATTAAATTGGATTTTAAGTTGTTTTAGTTCATTTGGTGAACTATC
TCGAATGACACAATTTAAAAATAAAAGAAAAAAAGAAAAAAACGATATTAAAAATATAAATATTGGACTATTAAATTATC
CTGTATTAATGTCATCAGATATTTTATTGTATCAAACAAATTTAGTACCTGTAGGACAAGATCAGAAACAGCATTTAGAA
TTAACTCGTAATATTGCTAATCGTTTTAATTTTTTGTATGGAGATATTTTTACATTACCCAATCCATTAATCAGTAAAAA
TGGATCTAAAATTATGTCTTTATTAGATCCTACAAAAAAAATGTCTAAATCAGATACTAATAAAAATAATGTAATTTTTT
TATTAGAAAATATTTCTTCAGTTTTTTTTAAAATAAAAAATGCTGTTACAGATTCAGAAACTCCGCCTAAAATATACTAT
GATGTAAAAAGAAAATTAGGTATTTCAAATTTATTAGAAATTCTTTCTGCTATTACGAATAAAGAAATTTTTATCTTAGA
AAAAGAATTCGATGGGATAATGTATAGTGAATTTAAAAATATTGTTTTTGATAATATATCTAAATTTTTGCATAAATTGC
AAAAATCTTACTTAATTTATCGAAAAGATGAATCTTACTTAAAAAATATTGCTAAAGAAGGAGCAATTAAAGCGAGATTA
AAATCTGAAAAAATTTTAAAACGTGTATTTTCTGCTGTAGAGATAGATTAA

Upstream 100 bases:

>100_bases
CTGATTATAATGTTATTATAAAAAAGATACGTAAAAAATTAAAAAACGTATATTCAACTTCTATTCATTGATTTTTTTTA
TATTTTTAGGATCATATAAT

Downstream 100 bases:

>100_bases
TTATTTAAATAAACTAGAAATTATTATTTATTCCAGTTTATTTAAAAAATAAAACTTTTTTAATAATATTTTTAACAATG
ATCTTTTTTTTTACTATTTT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MINSKPILFSAIQPSGNLTIGNYIGTMRYWSSFQDDYECLYCIADLHSLTTLNKNFSLKKSILDTLALYLACGVNPKKSI
IFIQSHVYQHSQLNWILSCFSSFGELSRMTQFKNKRKKEKNDIKNINIGLLNYPVLMSSDILLYQTNLVPVGQDQKQHLE
LTRNIANRFNFLYGDIFTLPNPLISKNGSKIMSLLDPTKKMSKSDTNKNNVIFLLENISSVFFKIKNAVTDSETPPKIYY
DVKRKLGISNLLEILSAITNKEIFILEKEFDGIMYSEFKNIVFDNISKFLHKLQKSYLIYRKDESYLKNIAKEGAIKARL
KSEKILKRVFSAVEID

Sequences:

>Translated_336_residues
MINSKPILFSAIQPSGNLTIGNYIGTMRYWSSFQDDYECLYCIADLHSLTTLNKNFSLKKSILDTLALYLACGVNPKKSI
IFIQSHVYQHSQLNWILSCFSSFGELSRMTQFKNKRKKEKNDIKNINIGLLNYPVLMSSDILLYQTNLVPVGQDQKQHLE
LTRNIANRFNFLYGDIFTLPNPLISKNGSKIMSLLDPTKKMSKSDTNKNNVIFLLENISSVFFKIKNAVTDSETPPKIYY
DVKRKLGISNLLEILSAITNKEIFILEKEFDGIMYSEFKNIVFDNISKFLHKLQKSYLIYRKDESYLKNIAKEGAIKARL
KSEKILKRVFSAVEID
>Mature_336_residues
MINSKPILFSAIQPSGNLTIGNYIGTMRYWSSFQDDYECLYCIADLHSLTTLNKNFSLKKSILDTLALYLACGVNPKKSI
IFIQSHVYQHSQLNWILSCFSSFGELSRMTQFKNKRKKEKNDIKNINIGLLNYPVLMSSDILLYQTNLVPVGQDQKQHLE
LTRNIANRFNFLYGDIFTLPNPLISKNGSKIMSLLDPTKKMSKSDTNKNNVIFLLENISSVFFKIKNAVTDSETPPKIYY
DVKRKLGISNLLEILSAITNKEIFILEKEFDGIMYSEFKNIVFDNISKFLHKLQKSYLIYRKDESYLKNIAKEGAIKARL
KSEKILKRVFSAVEID

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=318, Percent_Identity=38.0503144654088, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI41352700, Length=184, Percent_Identity=48.3695652173913, Blast_Score=188, Evalue=8e-48,
Organism=Escherichia coli, GI1789786, Length=330, Percent_Identity=58.1818181818182, Blast_Score=419, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71982800, Length=330, Percent_Identity=31.5151515151515, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71982793, Length=330, Percent_Identity=31.5151515151515, Blast_Score=167, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6320474, Length=342, Percent_Identity=38.3040935672515, Blast_Score=218, Evalue=9e-58,
Organism=Drosophila melanogaster, GI24666151, Length=327, Percent_Identity=35.1681957186544, Blast_Score=214, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_BUCAP (Q8K941)

Other databases:

- EMBL:   AE013218
- RefSeq:   NP_660849.1
- ProteinModelPortal:   Q8K941
- SMR:   Q8K941
- EnsemblBacteria:   EBBUCT00000000162
- GeneID:   1005545
- GenomeReviews:   AE013218_GR
- KEGG:   bas:BUsg517
- GeneTree:   EBGT00050000008055
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- ProtClustDB:   PRK00927
- BioCyc:   BAPH198804:BUSG517-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 38699; Mature: 38699

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 203-203

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSKPILFSAIQPSGNLTIGNYIGTMRYWSSFQDDYECLYCIADLHSLTTLNKNFSLKK
CCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
SILDTLALYLACGVNPKKSIIFIQSHVYQHSQLNWILSCFSSFGELSRMTQFKNKRKKEK
HHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDIKNINIGLLNYPVLMSSDILLYQTNLVPVGQDQKQHLELTRNIANRFNFLYGDIFTLP
HCCCCCEEEEEECCHHHCCCEEEEEECEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NPLISKNGSKIMSLLDPTKKMSKSDTNKNNVIFLLENISSVFFKIKNAVTDSETPPKIYY
CHHHHCCCHHHHHHHCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHH
DVKRKLGISNLLEILSAITNKEIFILEKEFDGIMYSEFKNIVFDNISKFLHKLQKSYLIY
HHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RKDESYLKNIAKEGAIKARLKSEKILKRVFSAVEID
ECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINSKPILFSAIQPSGNLTIGNYIGTMRYWSSFQDDYECLYCIADLHSLTTLNKNFSLKK
CCCCCCEEEEEECCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHH
SILDTLALYLACGVNPKKSIIFIQSHVYQHSQLNWILSCFSSFGELSRMTQFKNKRKKEK
HHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NDIKNINIGLLNYPVLMSSDILLYQTNLVPVGQDQKQHLELTRNIANRFNFLYGDIFTLP
HCCCCCEEEEEECCHHHCCCEEEEEECEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NPLISKNGSKIMSLLDPTKKMSKSDTNKNNVIFLLENISSVFFKIKNAVTDSETPPKIYY
CHHHHCCCHHHHHHHCHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCHHHH
DVKRKLGISNLLEILSAITNKEIFILEKEFDGIMYSEFKNIVFDNISKFLHKLQKSYLIY
HHHHHCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
RKDESYLKNIAKEGAIKARLKSEKILKRVFSAVEID
ECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438